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The map label for this gene is rlmN [H]

Identifier: 193212778

GI number: 193212778

Start: 1241261

End: 1242385

Strand: Direct

Name: rlmN [H]

Synonym: Cpar_1124

Alternate gene names: 193212778

Gene position: 1241261-1242385 (Clockwise)

Preceding gene: 193212773

Following gene: 193212779

Centisome position: 54.22

GC content: 58.13

Gene sequence:

>1125_bases
ATGCCGGAACCGTCTTTTTCTGGTGCATCCAAACCCTTTGACGACGCTCTGCCGAACATCAGGGAACTGAACCGGCAGGA
GCTTGAGCACCTCATGCAGCGCCTCGGTCAACCGGCCTACCGCGCCCGACAGCTGCACCAGTGGCTTTACAGCCATCAGG
CTCTCAGTTTCGAAGACATGACCTCGTTCGGCAAGAAGTTGCGCGAACAACTGGCGGGCTCCTGGGCCATCCGCCCAGCT
ACGCTTGATGCTACTGAAACCGAACCAGCCCAGTGCGCTGCACCCGGCGCTATTCCGACCTCCAAATTTCTCGTAAAGCT
GGATGACGGTGTGCTGATCGAAAGCGTCCTGATCCCTTCGGAAGAGCGCATGACCGCCTGCATCTCCTCGCAGGTCGGCT
GTGCGCTGCGCTGCACCTTCTGCGCCACCGGGCAGATGGGCTTCAAACGTGACCTCGTGGCCCCGGAAATCACCGATCAG
GTGTTCCTGCTCCAGCAGGAAGCCCACCGGCTGTATGGCCGGGGGATCACCAACACTGTATTCATGGGCATGGGCGAGCC
GCTGCTGAACCTCGACAACGTGTTCGAATCGATCTCCACGCTCACCGAGCAGGAGTACCGTTTCAGCATTTCCGAGCGGA
AAATCACCATCTCCACCGTAGGCCTCGTGCCGGAAATCGGCAGGATTGCCACATCAGGGCTCAAGACCAAGCTTGCAATC
TCGCTACACAGCGCAGACCAGGCCACGCGGGAGCGAATGATGCCGGTGGCAGCCGATTACCCGCTGGACGAACTCTCCAG
GGCAATCTCGGCCTACAACACCAAAACCGGCCAGCCGGTCACGCTCGTTTACATGCTGCTCGAGGGAATCAACGACGCCC
CTGAAGACGCGCGAAAACTGGCCCGTTTTGCAAAGCGGGGCTTGTGCAAAATTAATTTGATTGATTATAATGCAATCGTT
AATTTAAAGTTCAGGCCAGGTTACGGCAGCGCAAAATCCATGTTTATCGAGCGTTTGCTGGATGCCGGTCTGCACGTCAC
CGTCAGAAAAAGTCAGGGTGCTACCATCAACGCCGCTTGCGGCCAGTTAGCCACCAGGTCGGGTAACGACGGGGCGAAGC
CGTAG

Upstream 100 bases:

>100_bases
ATTTTCTCGGTTACTTATTACGACTATTACAAATCCGATAACAAGTATACAAAATAAAGTACTTTTTTTATCGGCTCATC
AAAAGAGAAGCAAAAGTTTT

Downstream 100 bases:

>100_bases
GACGCCGCAAAGACATGTCATGCTTGCAACTCTCCGGAGAAACAGCTTCAACCGGAACGCATGAGACCTTCTTAACCGTG
TCCCGCAGTAATTTTTAACA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MPEPSFSGASKPFDDALPNIRELNRQELEHLMQRLGQPAYRARQLHQWLYSHQALSFEDMTSFGKKLREQLAGSWAIRPA
TLDATETEPAQCAAPGAIPTSKFLVKLDDGVLIESVLIPSEERMTACISSQVGCALRCTFCATGQMGFKRDLVAPEITDQ
VFLLQQEAHRLYGRGITNTVFMGMGEPLLNLDNVFESISTLTEQEYRFSISERKITISTVGLVPEIGRIATSGLKTKLAI
SLHSADQATRERMMPVAADYPLDELSRAISAYNTKTGQPVTLVYMLLEGINDAPEDARKLARFAKRGLCKINLIDYNAIV
NLKFRPGYGSAKSMFIERLLDAGLHVTVRKSQGATINAACGQLATRSGNDGAKP

Sequences:

>Translated_374_residues
MPEPSFSGASKPFDDALPNIRELNRQELEHLMQRLGQPAYRARQLHQWLYSHQALSFEDMTSFGKKLREQLAGSWAIRPA
TLDATETEPAQCAAPGAIPTSKFLVKLDDGVLIESVLIPSEERMTACISSQVGCALRCTFCATGQMGFKRDLVAPEITDQ
VFLLQQEAHRLYGRGITNTVFMGMGEPLLNLDNVFESISTLTEQEYRFSISERKITISTVGLVPEIGRIATSGLKTKLAI
SLHSADQATRERMMPVAADYPLDELSRAISAYNTKTGQPVTLVYMLLEGINDAPEDARKLARFAKRGLCKINLIDYNAIV
NLKFRPGYGSAKSMFIERLLDAGLHVTVRKSQGATINAACGQLATRSGNDGAKP
>Mature_373_residues
PEPSFSGASKPFDDALPNIRELNRQELEHLMQRLGQPAYRARQLHQWLYSHQALSFEDMTSFGKKLREQLAGSWAIRPAT
LDATETEPAQCAAPGAIPTSKFLVKLDDGVLIESVLIPSEERMTACISSQVGCALRCTFCATGQMGFKRDLVAPEITDQV
FLLQQEAHRLYGRGITNTVFMGMGEPLLNLDNVFESISTLTEQEYRFSISERKITISTVGLVPEIGRIATSGLKTKLAIS
LHSADQATRERMMPVAADYPLDELSRAISAYNTKTGQPVTLVYMLLEGINDAPEDARKLARFAKRGLCKINLIDYNAIVN
LKFRPGYGSAKSMFIERLLDAGLHVTVRKSQGATINAACGQLATRSGNDGAKP

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=363, Percent_Identity=37.465564738292, Blast_Score=224, Evalue=7e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41158; Mature: 41027

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEPSFSGASKPFDDALPNIRELNRQELEHLMQRLGQPAYRARQLHQWLYSHQALSFEDM
CCCCCCCCCCCCHHHHCCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
TSFGKKLREQLAGSWAIRPATLDATETEPAQCAAPGAIPTSKFLVKLDDGVLIESVLIPS
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCCCCCCCCCEEEEECCCEEEHHEECCC
EERMTACISSQVGCALRCTFCATGQMGFKRDLVAPEITDQVFLLQQEAHRLYGRGITNTV
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
FMGMGEPLLNLDNVFESISTLTEQEYRFSISERKITISTVGLVPEIGRIATSGLKTKLAI
HHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCHHHHHHHHCCCCEEEEE
SLHSADQATRERMMPVAADYPLDELSRAISAYNTKTGQPVTLVYMLLEGINDAPEDARKL
EECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHH
ARFAKRGLCKINLIDYNAIVNLKFRPGYGSAKSMFIERLLDAGLHVTVRKSQGATINAAC
HHHHHCCCEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEHHH
GQLATRSGNDGAKP
HHHHCCCCCCCCCC
>Mature Secondary Structure 
PEPSFSGASKPFDDALPNIRELNRQELEHLMQRLGQPAYRARQLHQWLYSHQALSFEDM
CCCCCCCCCCCHHHHCCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
TSFGKKLREQLAGSWAIRPATLDATETEPAQCAAPGAIPTSKFLVKLDDGVLIESVLIPS
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCCCCCCCCCEEEEECCCEEEHHEECCC
EERMTACISSQVGCALRCTFCATGQMGFKRDLVAPEITDQVFLLQQEAHRLYGRGITNTV
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
FMGMGEPLLNLDNVFESISTLTEQEYRFSISERKITISTVGLVPEIGRIATSGLKTKLAI
HHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCHHHHHHHHCCCCEEEEE
SLHSADQATRERMMPVAADYPLDELSRAISAYNTKTGQPVTLVYMLLEGINDAPEDARKL
EECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHH
ARFAKRGLCKINLIDYNAIVNLKFRPGYGSAKSMFIERLLDAGLHVTVRKSQGATINAAC
HHHHHCCCEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEHHH
GQLATRSGNDGAKP
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]