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The map label for this gene is pleD [H]

Identifier: 193212648

GI number: 193212648

Start: 1097997

End: 1099286

Strand: Direct

Name: pleD [H]

Synonym: Cpar_0993

Alternate gene names: 193212648

Gene position: 1097997-1099286 (Clockwise)

Preceding gene: 193212647

Following gene: 193212649

Centisome position: 47.96

GC content: 55.66

Gene sequence:

>1290_bases
ATGAAACAGGAATACGAAACTTCCGGCAGAGGCTTGCTCCGACTGCCCATTATCACTGGTATTGCAGCGCTGCTGCTGGC
TTTCGGCGGCACGGCATGGTTCGGGGCTTACCAGACAGAGCATATCCGGGCTATTTCGCTCAAGCAGGATCTTCGCATGA
AGCTGGTCGAGTGCCGCACAACGGTTGCTTCGATCGAACAGCAGTTGGGGAGTGAGCAGCTCTCGCTTGCCCAGCGCGAG
GAGATTCAGTCGAGATTGCGCATCGAATGCGCCCGCTTTGCGGATGTGAAAAAGAATTCAGGCATCAAGCCGGAAAGCGA
GCTGGGGGAGTGGCTGATGAAAAGAAAGCCCATTACCAGCATTGTCAATCGGGAAACGCTTCAGCTTATCGTGGTCGATC
TGGACGAAATGATCGCGGCCATGACCAGCCAGATCGCTGAAGAGAACGATATGGTCGGCCTGAGCCTGATCTACTACCTC
GTATTCATCACCATTCTGCTGCTCTTTATCGTGCTTGCAGGCGGTCGCCTCCTCGCGTCAAACTATCGCCACTCGATCAT
TCCGCTCCACCAGCTTGCGCAACGCCTGATGCAGTTGAACCGCGATCTGCCCGAAAGCCTGCACGACACGGCCGAAGCCG
CCGAGGATATGCTGACCGGCCCCAACCCCTCCGCCGAAATGCGCTCCGTCACCAGCTCGGTAGCTTCGCTCTGCCACGAC
ATCGAGGCCAAAAACAAGAAGCTCGACGAAATTTACATTCGCGATGAAAAGACTGGCCTCTACAACTACCGCCACTTTAA
GGAGCACCTCATCATCGATGTGGAGCGGGCCAAGCGGTTTGGCGCCAGTATTGCGCTGGCCATGATCGACATCGACTACT
TCAAGATGTACAACGATCTGCACGGCCACATCGAGGGCGATAAGGTGCTTGTCAGGATCGCCGAAATCATCCACCAGGAG
TGCCGCGCGACCGACATTCCGTCACGGTTCGGTGGTGAGGAGTTCGCGCTGCTGTTCCCGAAAACCGATCGCCAGACAGC
GCTGGAGATTGCCGAACGGCTGCAAAGCATCATCAGCGCCGAACCCTTCGAGCACGAGCACACCCAGCCGGAAGGCCAGT
TGACCGTAAGCATCGGTGTGGCTGCGCTGCCTGATGATGCAACCGACTGGTATTCGTTGGTTAATAATGCCGATAAAGCT
CTTTACAAAGCAAAGTCTTTGGGCCGCAACAGAGTTTTAGGATTTACGAGGGAATTCTGTCAGGAACAACCGCCAGACAG
ACAGTGTTGA

Upstream 100 bases:

>100_bases
TTTGGCGCATGGCTCGCCAAGGAGGGGCAGAAGCTTTTCGAGCGCGGCGACATGGCTCCATACCAGCAGCTCGTGCGCAC
GATCATTATCAAATGAATGG

Downstream 100 bases:

>100_bases
CTTTAAACAGGGCAAAATGAGTTGATCGGTACAATTAACGTTTGAAAGGTTTGGATACCATGCTTGAAAAACTCATATCA
TTCGGGCCGTTTATAGCCAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKQEYETSGRGLLRLPIITGIAALLLAFGGTAWFGAYQTEHIRAISLKQDLRMKLVECRTTVASIEQQLGSEQLSLAQRE
EIQSRLRIECARFADVKKNSGIKPESELGEWLMKRKPITSIVNRETLQLIVVDLDEMIAAMTSQIAEENDMVGLSLIYYL
VFITILLLFIVLAGGRLLASNYRHSIIPLHQLAQRLMQLNRDLPESLHDTAEAAEDMLTGPNPSAEMRSVTSSVASLCHD
IEAKNKKLDEIYIRDEKTGLYNYRHFKEHLIIDVERAKRFGASIALAMIDIDYFKMYNDLHGHIEGDKVLVRIAEIIHQE
CRATDIPSRFGGEEFALLFPKTDRQTALEIAERLQSIISAEPFEHEHTQPEGQLTVSIGVAALPDDATDWYSLVNNADKA
LYKAKSLGRNRVLGFTREFCQEQPPDRQC

Sequences:

>Translated_429_residues
MKQEYETSGRGLLRLPIITGIAALLLAFGGTAWFGAYQTEHIRAISLKQDLRMKLVECRTTVASIEQQLGSEQLSLAQRE
EIQSRLRIECARFADVKKNSGIKPESELGEWLMKRKPITSIVNRETLQLIVVDLDEMIAAMTSQIAEENDMVGLSLIYYL
VFITILLLFIVLAGGRLLASNYRHSIIPLHQLAQRLMQLNRDLPESLHDTAEAAEDMLTGPNPSAEMRSVTSSVASLCHD
IEAKNKKLDEIYIRDEKTGLYNYRHFKEHLIIDVERAKRFGASIALAMIDIDYFKMYNDLHGHIEGDKVLVRIAEIIHQE
CRATDIPSRFGGEEFALLFPKTDRQTALEIAERLQSIISAEPFEHEHTQPEGQLTVSIGVAALPDDATDWYSLVNNADKA
LYKAKSLGRNRVLGFTREFCQEQPPDRQC
>Mature_429_residues
MKQEYETSGRGLLRLPIITGIAALLLAFGGTAWFGAYQTEHIRAISLKQDLRMKLVECRTTVASIEQQLGSEQLSLAQRE
EIQSRLRIECARFADVKKNSGIKPESELGEWLMKRKPITSIVNRETLQLIVVDLDEMIAAMTSQIAEENDMVGLSLIYYL
VFITILLLFIVLAGGRLLASNYRHSIIPLHQLAQRLMQLNRDLPESLHDTAEAAEDMLTGPNPSAEMRSVTSSVASLCHD
IEAKNKKLDEIYIRDEKTGLYNYRHFKEHLIIDVERAKRFGASIALAMIDIDYFKMYNDLHGHIEGDKVLVRIAEIIHQE
CRATDIPSRFGGEEFALLFPKTDRQTALEIAERLQSIISAEPFEHEHTQPEGQLTVSIGVAALPDDATDWYSLVNNADKA
LYKAKSLGRNRVLGFTREFCQEQPPDRQC

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=33.7349397590361, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081881, Length=195, Percent_Identity=36.4102564102564, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=31.8918918918919, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788085, Length=189, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=32.9113924050633, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787802, Length=132, Percent_Identity=37.8787878787879, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=32.2784810126582, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87082007, Length=135, Percent_Identity=34.8148148148148, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787816, Length=134, Percent_Identity=32.8358208955224, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=28.9308176100629, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=30.952380952381, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87081977, Length=151, Percent_Identity=29.8013245033113, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788956, Length=226, Percent_Identity=27.8761061946903, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 48512; Mature: 48512

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEYETSGRGLLRLPIITGIAALLLAFGGTAWFGAYQTEHIRAISLKQDLRMKLVECRT
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TVASIEQQLGSEQLSLAQREEIQSRLRIECARFADVKKNSGIKPESELGEWLMKRKPITS
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHH
IVNRETLQLIVVDLDEMIAAMTSQIAEENDMVGLSLIYYLVFITILLLFIVLAGGRLLAS
HHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
NYRHSIIPLHQLAQRLMQLNRDLPESLHDTAEAAEDMLTGPNPSAEMRSVTSSVASLCHD
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
IEAKNKKLDEIYIRDEKTGLYNYRHFKEHLIIDVERAKRFGASIALAMIDIDYFKMYNDL
HHHCCCCCCCEEEECCCCCCHHHHHHHHHHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHH
HGHIEGDKVLVRIAEIIHQECRATDIPSRFGGEEFALLFPKTDRQTALEIAERLQSIISA
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
EPFEHEHTQPEGQLTVSIGVAALPDDATDWYSLVNNADKALYKAKSLGRNRVLGFTREFC
CCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QEQPPDRQC
CCCCCCCCC
>Mature Secondary Structure
MKQEYETSGRGLLRLPIITGIAALLLAFGGTAWFGAYQTEHIRAISLKQDLRMKLVECRT
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TVASIEQQLGSEQLSLAQREEIQSRLRIECARFADVKKNSGIKPESELGEWLMKRKPITS
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHH
IVNRETLQLIVVDLDEMIAAMTSQIAEENDMVGLSLIYYLVFITILLLFIVLAGGRLLAS
HHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
NYRHSIIPLHQLAQRLMQLNRDLPESLHDTAEAAEDMLTGPNPSAEMRSVTSSVASLCHD
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
IEAKNKKLDEIYIRDEKTGLYNYRHFKEHLIIDVERAKRFGASIALAMIDIDYFKMYNDL
HHHCCCCCCCEEEECCCCCCHHHHHHHHHHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHH
HGHIEGDKVLVRIAEIIHQECRATDIPSRFGGEEFALLFPKTDRQTALEIAERLQSIISA
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
EPFEHEHTQPEGQLTVSIGVAALPDDATDWYSLVNNADKALYKAKSLGRNRVLGFTREFC
CCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QEQPPDRQC
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA