Click here to switch to the map view.

The map label for this gene is dut

Identifier: 193212411

GI number: 193212411

Start: 805521

End: 805973

Strand: Direct

Name: dut

Synonym: Cpar_0747

Alternate gene names: 193212411

Gene position: 805521-805973 (Clockwise)

Preceding gene: 193212408

Following gene: 193212417

Centisome position: 35.19

GC content: 59.6

Gene sequence:

>453_bases
ATGATCAAAGTAAAAATAGTTCGCCTGAAAGAAAAGGCAAGCCTGCCGGCTTATGCCACTGCTCACGCTGCGGGAATGGA
TGTTTCCGCCTGCCTCGATGCTCCAGTCACCCTCGAACCCTCATCGTCCGCGCTTATTCCCACCGGCCTCGCCATCGAGC
TGCCCGAGGGATACGAGGCGCAGCTTCGCCCGAGGAGCGGGCTGGCACTTCGCCACCTCATCTCGCTGCCCAATTCGCCT
GCAACCATCGATGCTGATTATCGCGGCGAAGTCGGGGTTATTCTCATCAATCACGGACGCGAGCCGTTTACGGTCAATCA
CGGCGACCGCATCGCGCAGATGGTCGTCTCAAAGGTTGATCGCGTCGCGTTCGAGGAGGTCGATTCACTGTCCGATACGG
AGCGCGGGGAAGGGGGATTCGGTCACACCGGCGTGGCCTCGAAAGCCGAGTAA

Upstream 100 bases:

>100_bases
TAAACAGGTTCGATGGGTGCGCTCAAGGTAGGGTTTGTTTATATTGCGGCTGAGTAACCATCAGGCCCTTCCGGGCTTCA
AGCAACTTCAGCCATAGTCG

Downstream 100 bases:

>100_bases
GCGATCCGGTTGATCTGACGGATCTGACTGATCGGGTGCAACGATTTCTCTGTTGCCAATTCCCAGTAGGGGGGCGCGAA
CCACCCGCATAAGGTTACGC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MIKVKIVRLKEKASLPAYATAHAAGMDVSACLDAPVTLEPSSSALIPTGLAIELPEGYEAQLRPRSGLALRHLISLPNSP
ATIDADYRGEVGVILINHGREPFTVNHGDRIAQMVVSKVDRVAFEEVDSLSDTERGEGGFGHTGVASKAE

Sequences:

>Translated_150_residues
MIKVKIVRLKEKASLPAYATAHAAGMDVSACLDAPVTLEPSSSALIPTGLAIELPEGYEAQLRPRSGLALRHLISLPNSP
ATIDADYRGEVGVILINHGREPFTVNHGDRIAQMVVSKVDRVAFEEVDSLSDTERGEGGFGHTGVASKAE
>Mature_150_residues
MIKVKIVRLKEKASLPAYATAHAAGMDVSACLDAPVTLEPSSSALIPTGLAIELPEGYEAQLRPRSGLALRHLISLPNSP
ATIDADYRGEVGVILINHGREPFTVNHGDRIAQMVVSKVDRVAFEEVDSLSDTERGEGGFGHTGVASKAE

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=143, Percent_Identity=44.0559440559441, Blast_Score=124, Evalue=3e-29,
Organism=Homo sapiens, GI4503423, Length=143, Percent_Identity=44.0559440559441, Blast_Score=124, Evalue=4e-29,
Organism=Homo sapiens, GI70906441, Length=143, Percent_Identity=44.0559440559441, Blast_Score=123, Evalue=7e-29,
Organism=Escherichia coli, GI1790071, Length=146, Percent_Identity=43.8356164383562, Blast_Score=123, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71988561, Length=143, Percent_Identity=41.958041958042, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6319729, Length=144, Percent_Identity=36.8055555555556, Blast_Score=97, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19921126, Length=151, Percent_Identity=40.3973509933775, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24583610, Length=151, Percent_Identity=40.3973509933775, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_CHLP8 (B3QML1)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001998364.1
- GeneID:   6419674
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0747
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- ProtClustDB:   CLSK637750
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15914; Mature: 15914

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: BINDING 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVKIVRLKEKASLPAYATAHAAGMDVSACLDAPVTLEPSSSALIPTGLAIELPEGYEA
CEEEEEEEEECCCCCCCEEEHHCCCCCHHHHCCCCEEECCCCCEECCCCEEEECCCCCCE
QLRPRSGLALRHLISLPNSPATIDADYRGEVGVILINHGREPFTVNHGDRIAQMVVSKVD
EECCCCCHHHHHHHHCCCCCCEEECCCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHH
RVAFEEVDSLSDTERGEGGFGHTGVASKAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKVKIVRLKEKASLPAYATAHAAGMDVSACLDAPVTLEPSSSALIPTGLAIELPEGYEA
CEEEEEEEEECCCCCCCEEEHHCCCCCHHHHCCCCEEECCCCCEECCCCEEEECCCCCCE
QLRPRSGLALRHLISLPNSPATIDADYRGEVGVILINHGREPFTVNHGDRIAQMVVSKVD
EECCCCCHHHHHHHHCCCCCCEEECCCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHH
RVAFEEVDSLSDTERGEGGFGHTGVASKAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA