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The map label for this gene is mdeA [H]

Identifier: 193212288

GI number: 193212288

Start: 665029

End: 666318

Strand: Direct

Name: mdeA [H]

Synonym: Cpar_0621

Alternate gene names: 193212288

Gene position: 665029-666318 (Clockwise)

Preceding gene: 193212285

Following gene: 193212289

Centisome position: 29.05

GC content: 60.7

Gene sequence:

>1290_bases
ATGACCAAGGACAACAGCTACCGGTTCGAGACTTTGCAGGTCCACGCCGGTCAGGAGCCCGATCCGGTGACCGGATCGCG
AGCGGTGCCGATTTACCAGACCACGTCGTATGTGTTCGAGAATGCCGAGCACGGGGCCGATCTGTTCGCGCTTCGCAAGC
CGGGCAACATCTACACGCGCCTGATGAACCCGACCACCGATGTGCTCGAAAAGCGCATGGCAGCGCTCGAAGGGGGCAAG
GCGGCCCTCGGCGTGGCGAGCGGCCACTCGGCGCAGTTCATCACCATCGCCACCATCTGTCAGGCGGGTGACAACATCGT
TTCGTCAAGCTCTCTGTACGGCGGCACCTACAACCAGTTCAAGGTGGCGTTCAAGCGTCTCGGTATCGAGGTGAAGTTCG
TGGACGTCAACGATCCGGAGGCGTTCAGGCGTGCTATCGACGAGAACACCAAGGCGCTGTACATGGAGTCGATCGGCAAC
CCGGCGTTCAACGTGCCCGATTTTGACGCGGTGGCCGCAATCGCGCGCGAGAACGGGATTCCGCTTATTGTCGATAATAC
CTTCGGCTGCGCGGGTTATCTCTGTCGCCCGATCGATCACGGCGCGTCCATCGTCGTCGAGTCGGCTACGAAATGGATCG
GCGGGCACGGTACCTCGATGGGTGGTATCATCGTCGATGCGGGCACGTTCGACTGGGGCAATGGCAAGTTTCCGCTCTTC
ACGGAGCCTTCGGAAGGGTACCACGGTCTGAAGTTTTACGAAGCGGTCGGCGATCTGGCCTTCATCGCGCGGGCGCGGGT
CGAGGGGCTGCGGGACTTCGGCCCGGCGATCAGCCCGTTCAACTCCTTCATGCTGTTGCAGGGCCTCGAAACCCTCTCGC
TCCGCGTTGAGCGTCACCTCGACAACACGCTCGCATTGGCCCGCTGGCTCCAGTCGCACGACGCGGTCGCTTGGGTGAAC
TATCCGGGTCTCGAAAGCCATCCGACCCACGAGCTGGCCAAAAAATACCTCACGCACGGTTTCGGTTGCGTGCTTACCTT
CGGTGTGAAGGGTGGCTACGAAAAAGCGGTGAAATTCATCGACAGCGTGAAGCTGGCCAGCCACCTGGCCAACGTTGGCG
ACGCCAAGACGCTGGTGATTCATCCCGCTTCGACGACGCATCAGCAGCTCAGCGCCGAAGAGCAGAAGTCGGCGGGCGTG
ACTGAGGACATGGTTCGCGTGTCGGTCGGCATCGAGCATATCGATGACATCAAGGCTGATTTCAGCCAGGCATTCGAGAA
TTTAGCATGA

Upstream 100 bases:

>100_bases
CCCCGTTGAGTTCATACTTCTCTTCATCGCTTCCCCTCGGTATAACCGATCCCTCACTTACCCCTGCTGCGCGAGAGCTT
CAAACCATCGATTTTTAGCC

Downstream 100 bases:

>100_bases
CCCAGATGGAGTATATACCGATCATTTCCGACAGTACCAGCTCCTTCAAATCATACGATCCTTTCCCGCTCGAACTGGGT
GGAGAGCTGCCGGAATTGAA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTKDNSYRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKPGNIYTRLMNPTTDVLEKRMAALEGGK
AALGVASGHSAQFITIATICQAGDNIVSSSSLYGGTYNQFKVAFKRLGIEVKFVDVNDPEAFRRAIDENTKALYMESIGN
PAFNVPDFDAVAAIARENGIPLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLF
TEPSEGYHGLKFYEAVGDLAFIARARVEGLRDFGPAISPFNSFMLLQGLETLSLRVERHLDNTLALARWLQSHDAVAWVN
YPGLESHPTHELAKKYLTHGFGCVLTFGVKGGYEKAVKFIDSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQKSAGV
TEDMVRVSVGIEHIDDIKADFSQAFENLA

Sequences:

>Translated_429_residues
MTKDNSYRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKPGNIYTRLMNPTTDVLEKRMAALEGGK
AALGVASGHSAQFITIATICQAGDNIVSSSSLYGGTYNQFKVAFKRLGIEVKFVDVNDPEAFRRAIDENTKALYMESIGN
PAFNVPDFDAVAAIARENGIPLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLF
TEPSEGYHGLKFYEAVGDLAFIARARVEGLRDFGPAISPFNSFMLLQGLETLSLRVERHLDNTLALARWLQSHDAVAWVN
YPGLESHPTHELAKKYLTHGFGCVLTFGVKGGYEKAVKFIDSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQKSAGV
TEDMVRVSVGIEHIDDIKADFSQAFENLA
>Mature_428_residues
TKDNSYRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKPGNIYTRLMNPTTDVLEKRMAALEGGKA
ALGVASGHSAQFITIATICQAGDNIVSSSSLYGGTYNQFKVAFKRLGIEVKFVDVNDPEAFRRAIDENTKALYMESIGNP
AFNVPDFDAVAAIARENGIPLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLFT
EPSEGYHGLKFYEAVGDLAFIARARVEGLRDFGPAISPFNSFMLLQGLETLSLRVERHLDNTLALARWLQSHDAVAWVNY
PGLESHPTHELAKKYLTHGFGCVLTFGVKGGYEKAVKFIDSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQKSAGVT
EDMVRVSVGIEHIDDIKADFSQAFENLA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=406, Percent_Identity=33.0049261083744, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI299473759, Length=406, Percent_Identity=29.5566502463054, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI25453487, Length=405, Percent_Identity=29.1358024691358, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1790375, Length=418, Percent_Identity=30.3827751196172, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1789383, Length=429, Percent_Identity=24.009324009324, Blast_Score=118, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=34.2789598108747, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17560056, Length=427, Percent_Identity=34.192037470726, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI71980708, Length=431, Percent_Identity=30.6264501160093, Blast_Score=197, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=59.2165898617512, Blast_Score=484, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6319307, Length=442, Percent_Identity=32.8054298642534, Blast_Score=189, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=443, Percent_Identity=27.0880361173815, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=353, Percent_Identity=26.9121813031161, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19922554, Length=433, Percent_Identity=32.7944572748268, Blast_Score=205, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46565; Mature: 46434

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDNSYRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKPGNIYTR
CCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCEEEEECCCCEEEE
LMNPTTDVLEKRMAALEGGKAALGVASGHSAQFITIATICQAGDNIVSSSSLYGGTYNQF
ECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCHHHH
KVAFKRLGIEVKFVDVNDPEAFRRAIDENTKALYMESIGNPAFNVPDFDAVAAIARENGI
HHHHHHHCCEEEEEECCCHHHHHHHHCCCCCEEEEHHCCCCCCCCCCHHHHHHHHHCCCC
PLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLF
EEEEECCCCCCHHHCCCCCCCCEEEEECHHHHHCCCCCCCCCEEEECCEEECCCCCCCEE
TEPSEGYHGLKFYEAVGDLAFIARARVEGLRDFGPAISPFNSFMLLQGLETLSLRVERHL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DNTLALARWLQSHDAVAWVNYPGLESHPTHELAKKYLTHGFGCVLTFGVKGGYEKAVKFI
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
DSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQKSAGVTEDMVRVSVGIEHIDDIKAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
FSQAFENLA
HHHHHHHCC
>Mature Secondary Structure 
TKDNSYRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKPGNIYTR
CCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCEEEEECCCCEEEE
LMNPTTDVLEKRMAALEGGKAALGVASGHSAQFITIATICQAGDNIVSSSSLYGGTYNQF
ECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCHHHH
KVAFKRLGIEVKFVDVNDPEAFRRAIDENTKALYMESIGNPAFNVPDFDAVAAIARENGI
HHHHHHHCCEEEEEECCCHHHHHHHHCCCCCEEEEHHCCCCCCCCCCHHHHHHHHHCCCC
PLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLF
EEEEECCCCCCHHHCCCCCCCCEEEEECHHHHHCCCCCCCCCEEEECCEEECCCCCCCEE
TEPSEGYHGLKFYEAVGDLAFIARARVEGLRDFGPAISPFNSFMLLQGLETLSLRVERHL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DNTLALARWLQSHDAVAWVNYPGLESHPTHELAKKYLTHGFGCVLTFGVKGGYEKAVKFI
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
DSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQKSAGVTEDMVRVSVGIEHIDDIKAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
FSQAFENLA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]