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The map label for this gene is tilS [H]

Identifier: 193212239

GI number: 193212239

Start: 615721

End: 616725

Strand: Direct

Name: tilS [H]

Synonym: Cpar_0572

Alternate gene names: 193212239

Gene position: 615721-616725 (Clockwise)

Preceding gene: 193212232

Following gene: 193212245

Centisome position: 26.9

GC content: 59.2

Gene sequence:

>1005_bases
ATGAATCGCACAGAAAAACAATTTCTTGAAAACATCCGGCGGCAGGGGATTGCCGGTGACGGCGAAGCGGTGCTCGTAGC
CGTTTCGGGTGGCCCGGATTCGATGGCGCTGCTGCACCTGCTGGTTGCAGCCGCTCCGGTGCTGCACCTGCGGCTTGGCG
TTGCGCACTGCAATTTCGGTCTGCGCGGCGAAGAGAGCGATGGCGATGAGGCGTTCGTCCGGTATGAGTGCGCTGGACTG
GGTCTGGATTTTCACGTGCGGCGATTCGACACGAAAGCCGTTGCTACGACGTGGAAAAAATCAATCGAGGAGACAGCCCG
CCTGCTGCGTTACGATTTCTTCGAGGAGTTGTGCCGGGAGAAAGGATACGAGCTTATCGCCACAGGTCATCACAGCGGCG
ACAATGCCGAAACCATGCTGTTCAATCTGTTTCGCGGCACGGCTGCTTCAGGGCTCCGAGGGATCAGGATGCGGCAGGGC
AGACTTATTCGCCCGCTGATTCCGTTCAGCCGTCAGGAGATCGTGAGCTACCTCGATGAGAAGAGCATCGCCTGGCGAAC
TGATCGCACCAATGCGGGGCTGGAGCACGATCGCAACTACATCCGTAACCGGGTGATTCCGGTTATCGAAGAGCGATTCA
GCCTCAAGCTTACGGCGTCCCTGCAACGGGTGGCGGAGCATGCTGGCGAGCTCGAAGAGTTCATGGAGCGGCATATCGAC
CGGTTGATCGAAGAGCGTCCTGGCCTCGATCCGGCAGGTGGCAAGCTGCATGTCGGCTCGCTGTTGGAGCTTTCGCGGTT
TGAACGGAAGGCGTTGATCAAGCGCGTGTTGCAGATGCGGGGACTGCCTGTTGAGAGCCGGGTACTGAGCCGGATTGCCG
GTCTGCTTGAGTCGCAGCCAGGCCGGCGTGTTCATGCCGGGCCTGGCGTTGATGTGGTCTGGAAGGATGGATTTCTGCGT
TTCGAACCGGTTATGCCGGACGATTCATCGAGCGGCAAGCATTAG

Upstream 100 bases:

>100_bases
TCGTTGGTGCGAGAAGAGTGTTATTGCAAACGGGAGGCGGGAGGTGCTATCTTGAACGAGCGAAACGAATTCTGTAAGCT
CGCATTGATAGAACGATTTA

Downstream 100 bases:

>100_bases
CGTCGTCACTCCTTGTGAGAGGTTTTTTTGAAGGCCTCCAGCGGAAGGTCGGCTGTGATGATGACCGATTCGAGGTTCTG
CTGCACTTTCATGTGGTGGA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MNRTEKQFLENIRRQGIAGDGEAVLVAVSGGPDSMALLHLLVAAAPVLHLRLGVAHCNFGLRGEESDGDEAFVRYECAGL
GLDFHVRRFDTKAVATTWKKSIEETARLLRYDFFEELCREKGYELIATGHHSGDNAETMLFNLFRGTAASGLRGIRMRQG
RLIRPLIPFSRQEIVSYLDEKSIAWRTDRTNAGLEHDRNYIRNRVIPVIEERFSLKLTASLQRVAEHAGELEEFMERHID
RLIEERPGLDPAGGKLHVGSLLELSRFERKALIKRVLQMRGLPVESRVLSRIAGLLESQPGRRVHAGPGVDVVWKDGFLR
FEPVMPDDSSSGKH

Sequences:

>Translated_334_residues
MNRTEKQFLENIRRQGIAGDGEAVLVAVSGGPDSMALLHLLVAAAPVLHLRLGVAHCNFGLRGEESDGDEAFVRYECAGL
GLDFHVRRFDTKAVATTWKKSIEETARLLRYDFFEELCREKGYELIATGHHSGDNAETMLFNLFRGTAASGLRGIRMRQG
RLIRPLIPFSRQEIVSYLDEKSIAWRTDRTNAGLEHDRNYIRNRVIPVIEERFSLKLTASLQRVAEHAGELEEFMERHID
RLIEERPGLDPAGGKLHVGSLLELSRFERKALIKRVLQMRGLPVESRVLSRIAGLLESQPGRRVHAGPGVDVVWKDGFLR
FEPVMPDDSSSGKH
>Mature_334_residues
MNRTEKQFLENIRRQGIAGDGEAVLVAVSGGPDSMALLHLLVAAAPVLHLRLGVAHCNFGLRGEESDGDEAFVRYECAGL
GLDFHVRRFDTKAVATTWKKSIEETARLLRYDFFEELCREKGYELIATGHHSGDNAETMLFNLFRGTAASGLRGIRMRQG
RLIRPLIPFSRQEIVSYLDEKSIAWRTDRTNAGLEHDRNYIRNRVIPVIEERFSLKLTASLQRVAEHAGELEEFMERHID
RLIEERPGLDPAGGKLHVGSLLELSRFERKALIKRVLQMRGLPVESRVLSRIAGLLESQPGRRVHAGPGVDVVWKDGFLR
FEPVMPDDSSSGKH

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=271, Percent_Identity=29.1512915129151, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 37627; Mature: 37627

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTEKQFLENIRRQGIAGDGEAVLVAVSGGPDSMALLHLLVAAAPVLHLRLGVAHCNFG
CCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LRGEESDGDEAFVRYECAGLGLDFHVRRFDTKAVATTWKKSIEETARLLRYDFFEELCRE
CCCCCCCCCCEEEEEEEECCCCEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGYELIATGHHSGDNAETMLFNLFRGTAASGLRGIRMRQGRLIRPLIPFSRQEIVSYLDE
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
KSIAWRTDRTNAGLEHDRNYIRNRVIPVIEERFSLKLTASLQRVAEHAGELEEFMERHID
CCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLIEERPGLDPAGGKLHVGSLLELSRFERKALIKRVLQMRGLPVESRVLSRIAGLLESQP
HHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GRRVHAGPGVDVVWKDGFLRFEPVMPDDSSSGKH
CCEEECCCCCEEEECCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNRTEKQFLENIRRQGIAGDGEAVLVAVSGGPDSMALLHLLVAAAPVLHLRLGVAHCNFG
CCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LRGEESDGDEAFVRYECAGLGLDFHVRRFDTKAVATTWKKSIEETARLLRYDFFEELCRE
CCCCCCCCCCEEEEEEEECCCCEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGYELIATGHHSGDNAETMLFNLFRGTAASGLRGIRMRQGRLIRPLIPFSRQEIVSYLDE
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
KSIAWRTDRTNAGLEHDRNYIRNRVIPVIEERFSLKLTASLQRVAEHAGELEEFMERHID
CCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLIEERPGLDPAGGKLHVGSLLELSRFERKALIKRVLQMRGLPVESRVLSRIAGLLESQP
HHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GRRVHAGPGVDVVWKDGFLRFEPVMPDDSSSGKH
CCEEECCCCCEEEECCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10976061; 12093901 [H]