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The map label for this gene is nifU [H]

Identifier: 193211916

GI number: 193211916

Start: 252294

End: 252905

Strand: Direct

Name: nifU [H]

Synonym: Cpar_0242

Alternate gene names: 193211916

Gene position: 252294-252905 (Clockwise)

Preceding gene: 193211915

Following gene: 193211917

Centisome position: 11.02

GC content: 56.54

Gene sequence:

>612_bases
ATGCTGCAATCAGGTGAATGGGCATATACGGAAAAGCTCAAGGAGCATTTCGAAAGTCCGAAAAATATTCTTCAGGGTAA
CGACACCAGTGAGTTCGACGGTGTCGGCATGGAGGGCAACCTCCAGTGTGGTGACCAGATGATGGTGGTCATCAAGGTGG
ACAAGGAGACCGAAAAGATCAACGACTGCCAGTGGAAAACCTACGGCTGTGCCAGCGCCATCGCCAGCACCTCCGTGCTG
TCCGAAATGGTCAAGGGCATGACCCTCGAAGAGGCGTTCGCCGTGTCGCCGAAGGATGTGACCAAGGAGCTCGGTGGTCT
GCCGGAGAACAAGATCCACTGCTCGGTGCTTGGCGACAAGGCGTTGCGTGCGGCGATCAACGACTACTATACGCGCAATG
GCATGGAGGATCGCGTTAAAAAGGAGCAGGCCAAGACCATCTGCCAGTGCATGAGCATCACCGACCACGACATCGAAGAG
GCGGTGCTCGAAGGCGCTCGCAGCTACTACGAGCTTCAGGAGCACACCAAGCTCGGCACCGTGTGCGGGCAGTGCAAGGA
TGAGGCCGAAGCCGAAATGCAGAAATTCATCCACCTGCACTTCGGAGCCTGA

Upstream 100 bases:

>100_bases
CCACCGTCGAAGAGGTCGAATACATGCTTGACGTCCTGCCGGGCGTCATCGAACGAATCAGAAACATGTCAACAGCTTAT
ATCTAATAAAGGAGGCGCTC

Downstream 100 bases:

>100_bases
CTGATTCTGTCGGAATCAGCATCGATTTTCAGTTCCTTTGACGGAGAGCGTGCTTCGTGATGCACCCGGTTTTCCGGGCA
ATTCACGAGGCGCGCCTCTT

Product: nitrogen-fixing NifU domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MLQSGEWAYTEKLKEHFESPKNILQGNDTSEFDGVGMEGNLQCGDQMMVVIKVDKETEKINDCQWKTYGCASAIASTSVL
SEMVKGMTLEEAFAVSPKDVTKELGGLPENKIHCSVLGDKALRAAINDYYTRNGMEDRVKKEQAKTICQCMSITDHDIEE
AVLEGARSYYELQEHTKLGTVCGQCKDEAEAEMQKFIHLHFGA

Sequences:

>Translated_203_residues
MLQSGEWAYTEKLKEHFESPKNILQGNDTSEFDGVGMEGNLQCGDQMMVVIKVDKETEKINDCQWKTYGCASAIASTSVL
SEMVKGMTLEEAFAVSPKDVTKELGGLPENKIHCSVLGDKALRAAINDYYTRNGMEDRVKKEQAKTICQCMSITDHDIEE
AVLEGARSYYELQEHTKLGTVCGQCKDEAEAEMQKFIHLHFGA
>Mature_203_residues
MLQSGEWAYTEKLKEHFESPKNILQGNDTSEFDGVGMEGNLQCGDQMMVVIKVDKETEKINDCQWKTYGCASAIASTSVL
SEMVKGMTLEEAFAVSPKDVTKELGGLPENKIHCSVLGDKALRAAINDYYTRNGMEDRVKKEQAKTICQCMSITDHDIEE
AVLEGARSYYELQEHTKLGTVCGQCKDEAEAEMQKFIHLHFGA

Specific function: Involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=128, Percent_Identity=44.53125, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI24307953, Length=115, Percent_Identity=46.9565217391304, Blast_Score=103, Evalue=8e-23,
Organism=Escherichia coli, GI1788878, Length=125, Percent_Identity=47.2, Blast_Score=112, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543474, Length=123, Percent_Identity=42.2764227642276, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6325122, Length=124, Percent_Identity=41.9354838709677, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324800, Length=132, Percent_Identity=36.3636363636364, Blast_Score=102, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21355597, Length=124, Percent_Identity=43.5483870967742, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 22644; Mature: 22644

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQSGEWAYTEKLKEHFESPKNILQGNDTSEFDGVGMEGNLQCGDQMMVVIKVDKETEKI
CCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHH
NDCQWKTYGCASAIASTSVLSEMVKGMTLEEAFAVSPKDVTKELGGLPENKIHCSVLGDK
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCCCEEEEEHHHH
ALRAAINDYYTRNGMEDRVKKEQAKTICQCMSITDHDIEEAVLEGARSYYELQEHTKLGT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VCGQCKDEAEAEMQKFIHLHFGA
HHHHCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLQSGEWAYTEKLKEHFESPKNILQGNDTSEFDGVGMEGNLQCGDQMMVVIKVDKETEKI
CCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHH
NDCQWKTYGCASAIASTSVLSEMVKGMTLEEAFAVSPKDVTKELGGLPENKIHCSVLGDK
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCCCEEEEEHHHH
ALRAAINDYYTRNGMEDRVKKEQAKTICQCMSITDHDIEEAVLEGARSYYELQEHTKLGT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VCGQCKDEAEAEMQKFIHLHFGA
HHHHCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672; 7947754; 10819462 [H]