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The map label for this gene is purA

Identifier: 193211891

GI number: 193211891

Start: 216867

End: 218174

Strand: Direct

Name: purA

Synonym: Cpar_0217

Alternate gene names: 193211891

Gene position: 216867-218174 (Clockwise)

Preceding gene: 193211890

Following gene: 193211892

Centisome position: 9.47

GC content: 60.63

Gene sequence:

>1308_bases
GTGGAAGAACGAACATTCAGCAGGCCGGCGGCGTCTGCAACTGTCCTGGTCGGTACGCAGTTCGGCGACGAGGGCAAGGG
CAAGCTGGTCGATTACCTCTCGGATCAATATGACATTGTAGTCCGCTACCAGGGCGGCGCAAACGCAGGCCACACCATCT
GCTTCGATGGCAAAACCGTGGTGCTTCACCTCATCCCCTCGGGCATTTTCAACAAGGATTGCATCTGCGTGATCGGCAAC
GGCGTGGTGATCGATCCGAACGCGCTGATGGAGGAGATCCAGAAGGTCGAAGAGCTGGGCTACGACGTGAAAGGCCGTCT
GTTCATCAGCCATAACGCGCACCTCATCATGCCGTACCACAAGCGGCTCGATTCGCTGAGCGAGTCCTGCCTCTCCGGCG
ATCAGAAGATCGGCACGACCGGTCGCGGTATCGGCCCCAGCTACGAGGACAAGTTCGCCCGCCACGGCATCCGGGTGGTC
GATCTGCTCAATCCGAAGGTGCTCGAAGAGAAGCTGCGCGACAACATCGAGGCCAAGAACAAGCTGATCTGCAAGGTCTA
CGAAAAAGAGGAAATCGACGTCGACGCGATCATCGGCGAGTACGAGGCGTTCGACAAGGTGATCGATCCCTACGTGACCA
ACACGCAGCTCTACCTGAACCGCCAGATCAAGGCGGGCAAGACCGTGCTGCTCGAAGGCGCGCAGGGTTGCCTGCTCGAC
GTGGATCACGGCACCTACCCGTTCGTCACCTCTTCCAACCCGACCTCCGGCGGTGCGTGCACCGGCTCCGGCGTCGCGCC
GAACCACATCGGCAAGGTGATCGGCGTCTGCAAGGCCTACATGACCCGCGTCGGCAACGGCGATTTCCCGACCGAGCTTG
AGGACGAGACCGGCGAAAAGCTGGGCGAGATCGGCCACGAGTTCGGCGCAACGACCGGGCGCAAGCGCCGTTGCGGCTGG
CTCGATCTTGTGGCGCTGCGCTACTCGCTCACCATCAGCGGCGTGACCGAGCTGGCGCTGACCAAGCTCGACGTGCTCGA
CACTTTCGAGGAGATCAAGGTGTGCACCTCCTACATGCTCGACGGCAAGGAGATTTTCGACTTCCCGACCGAGCACCAGA
CCCTCTGTCGCGTGCAGCCGGTTTACAAGAGCCTCAAGGGCTGGATGGCCTCGAACGCCAATGCGAAGAGCTTCGCTGAG
ATGCATCCAAACGCCCAGGCCTATGTCAACTTTCTCGAAGAGGCGACAGGTGTGCCGGTGACCTTTATCTCTGTAGGCCC
CGGTCGCGACGAGACGGTGTTCAAATAA

Upstream 100 bases:

>100_bases
CGAATGCCAGATTTATTGTAAATTACTCGTCTTTGTCGGGCGCGGGCCGGGACAAGCTTCTGGAGCGCCTCGATCTTTTT
TCTCAGTAAAAGCAAGCGAC

Downstream 100 bases:

>100_bases
GAGATTGGGCTGAAGCCCTGAGTTGTTTTTTGGGATTCCGTGTCCCCGGCTTGAAAGCCGGGGCAATTGAAATGTAAGAG
GGGTTGAATGTCCGTCGGGG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MEERTFSRPAASATVLVGTQFGDEGKGKLVDYLSDQYDIVVRYQGGANAGHTICFDGKTVVLHLIPSGIFNKDCICVIGN
GVVIDPNALMEEIQKVEELGYDVKGRLFISHNAHLIMPYHKRLDSLSESCLSGDQKIGTTGRGIGPSYEDKFARHGIRVV
DLLNPKVLEEKLRDNIEAKNKLICKVYEKEEIDVDAIIGEYEAFDKVIDPYVTNTQLYLNRQIKAGKTVLLEGAQGCLLD
VDHGTYPFVTSSNPTSGGACTGSGVAPNHIGKVIGVCKAYMTRVGNGDFPTELEDETGEKLGEIGHEFGATTGRKRRCGW
LDLVALRYSLTISGVTELALTKLDVLDTFEEIKVCTSYMLDGKEIFDFPTEHQTLCRVQPVYKSLKGWMASNANAKSFAE
MHPNAQAYVNFLEEATGVPVTFISVGPGRDETVFK

Sequences:

>Translated_435_residues
MEERTFSRPAASATVLVGTQFGDEGKGKLVDYLSDQYDIVVRYQGGANAGHTICFDGKTVVLHLIPSGIFNKDCICVIGN
GVVIDPNALMEEIQKVEELGYDVKGRLFISHNAHLIMPYHKRLDSLSESCLSGDQKIGTTGRGIGPSYEDKFARHGIRVV
DLLNPKVLEEKLRDNIEAKNKLICKVYEKEEIDVDAIIGEYEAFDKVIDPYVTNTQLYLNRQIKAGKTVLLEGAQGCLLD
VDHGTYPFVTSSNPTSGGACTGSGVAPNHIGKVIGVCKAYMTRVGNGDFPTELEDETGEKLGEIGHEFGATTGRKRRCGW
LDLVALRYSLTISGVTELALTKLDVLDTFEEIKVCTSYMLDGKEIFDFPTEHQTLCRVQPVYKSLKGWMASNANAKSFAE
MHPNAQAYVNFLEEATGVPVTFISVGPGRDETVFK
>Mature_435_residues
MEERTFSRPAASATVLVGTQFGDEGKGKLVDYLSDQYDIVVRYQGGANAGHTICFDGKTVVLHLIPSGIFNKDCICVIGN
GVVIDPNALMEEIQKVEELGYDVKGRLFISHNAHLIMPYHKRLDSLSESCLSGDQKIGTTGRGIGPSYEDKFARHGIRVV
DLLNPKVLEEKLRDNIEAKNKLICKVYEKEEIDVDAIIGEYEAFDKVIDPYVTNTQLYLNRQIKAGKTVLLEGAQGCLLD
VDHGTYPFVTSSNPTSGGACTGSGVAPNHIGKVIGVCKAYMTRVGNGDFPTELEDETGEKLGEIGHEFGATTGRKRRCGW
LDLVALRYSLTISGVTELALTKLDVLDTFEEIKVCTSYMLDGKEIFDFPTEHQTLCRVQPVYKSLKGWMASNANAKSFAE
MHPNAQAYVNFLEEATGVPVTFISVGPGRDETVFK

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=45.3271028037383, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI34577063, Length=441, Percent_Identity=44.8979591836735, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=44.6700507614213, Blast_Score=326, Evalue=3e-89,
Organism=Escherichia coli, GI1790620, Length=421, Percent_Identity=47.5059382422803, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=44.6759259259259, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=44.6759259259259, Blast_Score=359, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=45.2655889145497, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=44.3127962085308, Blast_Score=354, Evalue=5e-98,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_CHLP8 (B3QQZ0)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001997844.1
- GeneID:   6419125
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_0217
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47738; Mature: 47738

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 23-23 ACT_SITE 51-51 BINDING 140-140 BINDING 154-154 BINDING 235-235 BINDING 250-250 BINDING 314-314 BINDING 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERTFSRPAASATVLVGTQFGDEGKGKLVDYLSDQYDIVVRYQGGANAGHTICFDGKTV
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEEECCCEE
VLHLIPSGIFNKDCICVIGNGVVIDPNALMEEIQKVEELGYDVKGRLFISHNAHLIMPYH
EEEEECCCCCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHH
KRLDSLSESCLSGDQKIGTTGRGIGPSYEDKFARHGIRVVDLLNPKVLEEKLRDNIEAKN
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCC
KLICKVYEKEEIDVDAIIGEYEAFDKVIDPYVTNTQLYLNRQIKAGKTVLLEGAQGCLLD
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCEEE
VDHGTYPFVTSSNPTSGGACTGSGVAPNHIGKVIGVCKAYMTRVGNGDFPTELEDETGEK
CCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LGEIGHEFGATTGRKRRCGWLDLVALRYSLTISGVTELALTKLDVLDTFEEIKVCTSYML
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGKEIFDFPTEHQTLCRVQPVYKSLKGWMASNANAKSFAEMHPNAQAYVNFLEEATGVPV
CCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCE
TFISVGPGRDETVFK
EEEEECCCCCCCCCC
>Mature Secondary Structure
MEERTFSRPAASATVLVGTQFGDEGKGKLVDYLSDQYDIVVRYQGGANAGHTICFDGKTV
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEEECCCEE
VLHLIPSGIFNKDCICVIGNGVVIDPNALMEEIQKVEELGYDVKGRLFISHNAHLIMPYH
EEEEECCCCCCCCEEEEECCCEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHH
KRLDSLSESCLSGDQKIGTTGRGIGPSYEDKFARHGIRVVDLLNPKVLEEKLRDNIEAKN
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCC
KLICKVYEKEEIDVDAIIGEYEAFDKVIDPYVTNTQLYLNRQIKAGKTVLLEGAQGCLLD
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCEEE
VDHGTYPFVTSSNPTSGGACTGSGVAPNHIGKVIGVCKAYMTRVGNGDFPTELEDETGEK
CCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LGEIGHEFGATTGRKRRCGWLDLVALRYSLTISGVTELALTKLDVLDTFEEIKVCTSYML
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGKEIFDFPTEHQTLCRVQPVYKSLKGWMASNANAKSFAEMHPNAQAYVNFLEEATGVPV
CCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCE
TFISVGPGRDETVFK
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA