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The map label for this gene is ispE

Identifier: 193214438

GI number: 193214438

Start: 846500

End: 847357

Strand: Direct

Name: ispE

Synonym: Ctha_0721

Alternate gene names: 193214438

Gene position: 846500-847357 (Clockwise)

Preceding gene: 193214437

Following gene: 193214439

Centisome position: 25.7

GC content: 49.42

Gene sequence:

>858_bases
ATGTCAAAAACAGTCAATGCTTACGCCAAAATTAATTTGGCGCTTTTCATCACGGGCAAATTGCCCAATGGCTACCACAC
ACTGGAAACCATTTTTGCCCCGATTAATTGGTATGACCGCCTGACTTTTGAGCCTTCCGAAACAATTGAAATGGCCTGCA
CAAATGCTGAACTTCCCACCGACGATTCAAATCTTTGCATAAAGGCCGCCAAACGCTTGCAAACCGAAAGCGGCTCGCAA
AAAGGCGTGAAAATTTCGCTTGAGAAAAATGTGCCTTTTGGCGCGGGGCTTGGCGGCGGCAGCAGCGACGCGGCAGCAAC
TTTAAACGCATTGAATGAACTTTGGGAACTTTCGCTTCCAAGCGAAACGCTCCATAAGCTCGCCACCGAACTTGGCGCGG
ATGTCCCTTATTTTCTTGAAATGCCCGCACTCGCTCTTGGCACGGGAATTGGCGAAGAACTGACCGATTTGCAAGTCGCG
TTTCCATTTTCCATCGTAACGGTTTTTCCTGAAACAGCGATTTCAACCGCGTGGGCTTATCAAAATTTCAAGCAGAATTT
TGACCGACTGTTGCCCGACGCCGCAACCGAAATTCAGATTGTTTGCGAAACCGGAGACTTGGGGCGAATGCAGCAATTCG
AGAACGATTTTGAATCGATCGTCTATGAAAATTATGCAGAGGTGAAAAAATTGCGCGATGACTTCGTTGAAGCTGGCAGC
GGATTCACGCGGCTTTCCGGCAGCGGGTCGGCGGTGTTCGGCGTTTTTGCGGATGACGAAAAAGCGACGGCGTGCTACGA
GGCAATGAGAAAACGCTACCCAACCTCGCTGACGCCAAAGTCCTTCAAAATGAAGTGA

Upstream 100 bases:

>100_bases
ATTTCCCTCACTTAACTCTGATGATTGTTTTGTATTTTAATTTTCAAAATTTCCCTGGCATCAGCATTGCCAATAATTTT
TCTGAAGAAAAAAATTTGCC

Downstream 100 bases:

>100_bases
CCCGAATGAGCGCGAAAAATCAGGTTTTTGAGAAATTGTTGGCGGCCAAAGCGCGAAAAGGCGCAGGGTTTTTGCTTTTA
ATCGACCCCGATAAATATGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSKTVNAYAKINLALFITGKLPNGYHTLETIFAPINWYDRLTFEPSETIEMACTNAELPTDDSNLCIKAAKRLQTESGSQ
KGVKISLEKNVPFGAGLGGGSSDAAATLNALNELWELSLPSETLHKLATELGADVPYFLEMPALALGTGIGEELTDLQVA
FPFSIVTVFPETAISTAWAYQNFKQNFDRLLPDAATEIQIVCETGDLGRMQQFENDFESIVYENYAEVKKLRDDFVEAGS
GFTRLSGSGSAVFGVFADDEKATACYEAMRKRYPTSLTPKSFKMK

Sequences:

>Translated_285_residues
MSKTVNAYAKINLALFITGKLPNGYHTLETIFAPINWYDRLTFEPSETIEMACTNAELPTDDSNLCIKAAKRLQTESGSQ
KGVKISLEKNVPFGAGLGGGSSDAAATLNALNELWELSLPSETLHKLATELGADVPYFLEMPALALGTGIGEELTDLQVA
FPFSIVTVFPETAISTAWAYQNFKQNFDRLLPDAATEIQIVCETGDLGRMQQFENDFESIVYENYAEVKKLRDDFVEAGS
GFTRLSGSGSAVFGVFADDEKATACYEAMRKRYPTSLTPKSFKMK
>Mature_284_residues
SKTVNAYAKINLALFITGKLPNGYHTLETIFAPINWYDRLTFEPSETIEMACTNAELPTDDSNLCIKAAKRLQTESGSQK
GVKISLEKNVPFGAGLGGGSSDAAATLNALNELWELSLPSETLHKLATELGADVPYFLEMPALALGTGIGEELTDLQVAF
PFSIVTVFPETAISTAWAYQNFKQNFDRLLPDAATEIQIVCETGDLGRMQQFENDFESIVYENYAEVKKLRDDFVEAGSG
FTRLSGSGSAVFGVFADDEKATACYEAMRKRYPTSLTPKSFKMK

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=266, Percent_Identity=33.4586466165414, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CHLT3 (B3QW77)

Other databases:

- EMBL:   CP001100
- RefSeq:   YP_001995637.1
- ProteinModelPortal:   B3QW77
- GeneID:   6421766
- GenomeReviews:   CP001100_GR
- KEGG:   cts:Ctha_0721
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK14616
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31226; Mature: 31094

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTVNAYAKINLALFITGKLPNGYHTLETIFAPINWYDRLTFEPSETIEMACTNAELPT
CCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHCCCHHHCEECCCCCCEEEEECCCCCCC
DDSNLCIKAAKRLQTESGSQKGVKISLEKNVPFGAGLGGGSSDAAATLNALNELWELSLP
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SETLHKLATELGADVPYFLEMPALALGTGIGEELTDLQVAFPFSIVTVFPETAISTAWAY
HHHHHHHHHHHCCCCCHHHHCCHHHHHCCCCCHHHHEEEECCEEEEEECCHHHHHHHHHH
QNFKQNFDRLLPDAATEIQIVCETGDLGRMQQFENDFESIVYENYAEVKKLRDDFVEAGS
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GFTRLSGSGSAVFGVFADDEKATACYEAMRKRYPTSLTPKSFKMK
CEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SKTVNAYAKINLALFITGKLPNGYHTLETIFAPINWYDRLTFEPSETIEMACTNAELPT
CCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHCCCHHHCEECCCCCCEEEEECCCCCCC
DDSNLCIKAAKRLQTESGSQKGVKISLEKNVPFGAGLGGGSSDAAATLNALNELWELSLP
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SETLHKLATELGADVPYFLEMPALALGTGIGEELTDLQVAFPFSIVTVFPETAISTAWAY
HHHHHHHHHHHCCCCCHHHHCCHHHHHCCCCCHHHHEEEECCEEEEEECCHHHHHHHHHH
QNFKQNFDRLLPDAATEIQIVCETGDLGRMQQFENDFESIVYENYAEVKKLRDDFVEAGS
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GFTRLSGSGSAVFGVFADDEKATACYEAMRKRYPTSLTPKSFKMK
CEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA