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The map label for this gene is relA [H]

Identifier: 193214224

GI number: 193214224

Start: 597081

End: 599315

Strand: Direct

Name: relA [H]

Synonym: Ctha_0505

Alternate gene names: 193214224

Gene position: 597081-599315 (Clockwise)

Preceding gene: 193214220

Following gene: 193214226

Centisome position: 18.13

GC content: 46.04

Gene sequence:

>2235_bases
ATGTTAGCGCAAATTGAGGAAGATCATTATGTAAAGCTGAACGAAATTTTGTCCTTATGTAAAGAAAAGCTTTACCATTA
CGATGAAGCCTTGATTCGCAAGGCGTTCTTTTTATGCTACCGGGCGCACAGCGAAGAAAAGCGCGCGTCAGGCGAGCCTT
TCTTTTTTCACCCCGTTGAAGTGGCAAAAATTCTTTTGACGGAAATTCCGCTCGATAGCGTTTCCGTAGCCGCTGCCCTG
CTGCACGACGTGGTCGAAGATACCGACTACACCTACGAGGATTTACAAGACGAGTTTGGCGATGAAATTGCGAGCATTGT
CGCCGGCTTAACCAAAATTTCCGGCATTTTTGATAATCGGGAAATTAAAGAAGCCGAAAGCTTTCGGAAAATGTTGCTTT
CGGTGATTCACGACATTCGCGTCATTCTTATCAAATTCTGCGATCGCCTTCATAACATGCGCACGCTCGATGCGCTTCCG
CCGCATCGCCAAATCCGCATTGCCATCGAAACGCGCGACATTTACGCGCCATTGGCTCACCGCTTTGGCGTTGGGCGCAT
TAAAATTGAGCTCGAAAATTTAGCGCTAAAATATATCGACCGCGAAGCCTACGACGAGATCGTTCAAAAGCTGCAAATGA
CACGCCCCGAACGCGAGCGTTATTTGGAATCTGTGATGTCGCCGATTCGCAAAAAACTCAACGAGCTGAATTTTGAGTAC
AACATGTCGGGACGCCCAAAACATGTTTTTTCCATTTACAATAAAATCCAGCAGCGAGGCAAATCCTTTGAGGACATTTA
CGACATTTATGGCATTCGCATCATTTTGCATTCCGAGACGAACGCAGATTGCTACAATGTGTATAGCCGCATTACGGAGC
TTTTTTCGCCCGTGCCCGATAGATTCAAGGATTTTATTAGCGTGCCGAAACACAACGGCTACGCGTCGCTCCACACCACG
ATTGTTGGCCCTGGCGGACGCATGATTGAGGTGCAAATTCGCACCGAAAAAATGAACGAAATTGCTGAAACCGGTATCGC
GGCGCACTGGCGCTATAAGGAAAAAACCAAAGAGCGCGACAAGGCTATCGACGAACTCATCAAGTGGGCTCGCGAATTTA
TTGAAAATTCGTCGATGTCTTCCACTGTGATGGACTCCGACTCAGCAACCAGCTTCATGGAAGGCTTTCGGATGCACCTT
TACCAGGATGAAATTTATGTCTTCACCCCAAAAGGAGACATGAAAACCATGCCGGTTAATGCCACACCTGTTGATTTTGC
GTTTGAAATTCACACGGAAGTCGGAATGCGCTGCATTGGCGCCAAGGTCAATGGAAAGATCGTCTCGCTCAATTCCAAAC
TAAAATCCGGCGATCAGGTTGAAATTATCACCTCTAAAAATCAAACGCCAAAAGCAGATTGGGAAAAATTTGTCGTTACA
CACAAGGCCAAAATTAAAATTAAGCAGGCGCTCAATGAAGAACGGCGAACTGAAATTGAGAAAGGACGCGGCATTTGGAA
TCGCGTATTGACAAAAACGAAAAGCATTATCGCCCTCACCGAGCGCGATTTGCTAAAAGCGATCAAAGAACACGGATTCC
ACTTTAACACGCCCGCCGATTTTTATAGAGGGCTGGCTACGGATAAAATCAACGCCGAGGAGGTTTTAAATAACCTGAAT
GAAAAGAAAAAATCTCCGCCTCAAAAAGAATTGACACCGCTAACTGGCGAGGCGCAAGCAAACACGCTGAAAAAGCTCCG
CGAACCGTCAATCAACCAAAAAAACAAAGATGCCGTCAGAATCGAAGGCCTGCCAGGCATCGCCTTCGAGTATGCGCGCT
GTTGCAACCCAGTTCCCGGCGACGACATTATTGGGCTTTTATCCGTTGGCAGCAAAGCGGTGAAAATTCACAGAAGAAAT
TGCCGAAGTCTTACATCCGATAAGCTCGCCACCATGAAGCTCCTGGATGCATGTTGGGAAAAAGACAACAACGGGCAGTT
TTTAGCTGGGCTAAAAATTCTTGGCGAGGATGTGATTGGCATGACAAATAAAATCACAGAGGTCATTTTAAAATCGGATA
TTAACATTCGATCGATTTCGTTAGACGCGAGCGATTCTTTATTTGAAGGCAATGTGCTGCTTTATGTCAAAGATGTGGCC
ACATTAAATCGTTTGGCTGAAAAAATAAAGCGCATCAAAGGAATTTTGAGCGTAGGCCGGCTTTCGAACGAGTAA

Upstream 100 bases:

>100_bases
AAAACTGATACGATAGGCAAGCATTTGCGTGTTCTGAGCCGTGCAATAATCTGGGCGGTGCAAGAAGGTTGTTTGAAATT
ATCAATGTTTGGAGGAGATT

Downstream 100 bases:

>100_bases
TGGGAAATGCTCATTCATGTTCGCCAATGATCAAATAATCCTTGATTTTCTCATACATGCCTGGGCTAATGCCGCGCACA
TTTTCGAGTTCGGAGATTGT

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MLAQIEEDHYVKLNEILSLCKEKLYHYDEALIRKAFFLCYRAHSEEKRASGEPFFFHPVEVAKILLTEIPLDSVSVAAAL
LHDVVEDTDYTYEDLQDEFGDEIASIVAGLTKISGIFDNREIKEAESFRKMLLSVIHDIRVILIKFCDRLHNMRTLDALP
PHRQIRIAIETRDIYAPLAHRFGVGRIKIELENLALKYIDREAYDEIVQKLQMTRPERERYLESVMSPIRKKLNELNFEY
NMSGRPKHVFSIYNKIQQRGKSFEDIYDIYGIRIILHSETNADCYNVYSRITELFSPVPDRFKDFISVPKHNGYASLHTT
IVGPGGRMIEVQIRTEKMNEIAETGIAAHWRYKEKTKERDKAIDELIKWAREFIENSSMSSTVMDSDSATSFMEGFRMHL
YQDEIYVFTPKGDMKTMPVNATPVDFAFEIHTEVGMRCIGAKVNGKIVSLNSKLKSGDQVEIITSKNQTPKADWEKFVVT
HKAKIKIKQALNEERRTEIEKGRGIWNRVLTKTKSIIALTERDLLKAIKEHGFHFNTPADFYRGLATDKINAEEVLNNLN
EKKKSPPQKELTPLTGEAQANTLKKLREPSINQKNKDAVRIEGLPGIAFEYARCCNPVPGDDIIGLLSVGSKAVKIHRRN
CRSLTSDKLATMKLLDACWEKDNNGQFLAGLKILGEDVIGMTNKITEVILKSDINIRSISLDASDSLFEGNVLLYVKDVA
TLNRLAEKIKRIKGILSVGRLSNE

Sequences:

>Translated_744_residues
MLAQIEEDHYVKLNEILSLCKEKLYHYDEALIRKAFFLCYRAHSEEKRASGEPFFFHPVEVAKILLTEIPLDSVSVAAAL
LHDVVEDTDYTYEDLQDEFGDEIASIVAGLTKISGIFDNREIKEAESFRKMLLSVIHDIRVILIKFCDRLHNMRTLDALP
PHRQIRIAIETRDIYAPLAHRFGVGRIKIELENLALKYIDREAYDEIVQKLQMTRPERERYLESVMSPIRKKLNELNFEY
NMSGRPKHVFSIYNKIQQRGKSFEDIYDIYGIRIILHSETNADCYNVYSRITELFSPVPDRFKDFISVPKHNGYASLHTT
IVGPGGRMIEVQIRTEKMNEIAETGIAAHWRYKEKTKERDKAIDELIKWAREFIENSSMSSTVMDSDSATSFMEGFRMHL
YQDEIYVFTPKGDMKTMPVNATPVDFAFEIHTEVGMRCIGAKVNGKIVSLNSKLKSGDQVEIITSKNQTPKADWEKFVVT
HKAKIKIKQALNEERRTEIEKGRGIWNRVLTKTKSIIALTERDLLKAIKEHGFHFNTPADFYRGLATDKINAEEVLNNLN
EKKKSPPQKELTPLTGEAQANTLKKLREPSINQKNKDAVRIEGLPGIAFEYARCCNPVPGDDIIGLLSVGSKAVKIHRRN
CRSLTSDKLATMKLLDACWEKDNNGQFLAGLKILGEDVIGMTNKITEVILKSDINIRSISLDASDSLFEGNVLLYVKDVA
TLNRLAEKIKRIKGILSVGRLSNE
>Mature_744_residues
MLAQIEEDHYVKLNEILSLCKEKLYHYDEALIRKAFFLCYRAHSEEKRASGEPFFFHPVEVAKILLTEIPLDSVSVAAAL
LHDVVEDTDYTYEDLQDEFGDEIASIVAGLTKISGIFDNREIKEAESFRKMLLSVIHDIRVILIKFCDRLHNMRTLDALP
PHRQIRIAIETRDIYAPLAHRFGVGRIKIELENLALKYIDREAYDEIVQKLQMTRPERERYLESVMSPIRKKLNELNFEY
NMSGRPKHVFSIYNKIQQRGKSFEDIYDIYGIRIILHSETNADCYNVYSRITELFSPVPDRFKDFISVPKHNGYASLHTT
IVGPGGRMIEVQIRTEKMNEIAETGIAAHWRYKEKTKERDKAIDELIKWAREFIENSSMSSTVMDSDSATSFMEGFRMHL
YQDEIYVFTPKGDMKTMPVNATPVDFAFEIHTEVGMRCIGAKVNGKIVSLNSKLKSGDQVEIITSKNQTPKADWEKFVVT
HKAKIKIKQALNEERRTEIEKGRGIWNRVLTKTKSIIALTERDLLKAIKEHGFHFNTPADFYRGLATDKINAEEVLNNLN
EKKKSPPQKELTPLTGEAQANTLKKLREPSINQKNKDAVRIEGLPGIAFEYARCCNPVPGDDIIGLLSVGSKAVKIHRRN
CRSLTSDKLATMKLLDACWEKDNNGQFLAGLKILGEDVIGMTNKITEVILKSDINIRSISLDASDSLFEGNVLLYVKDVA
TLNRLAEKIKRIKGILSVGRLSNE

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=715, Percent_Identity=35.2447552447552, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1789147, Length=727, Percent_Identity=28.8858321870702, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25143118, Length=137, Percent_Identity=35.036496350365, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 85051; Mature: 85051

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQIEEDHYVKLNEILSLCKEKLYHYDEALIRKAFFLCYRAHSEEKRASGEPFFFHPVE
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEECHHH
VAKILLTEIPLDSVSVAAALLHDVVEDTDYTYEDLQDEFGDEIASIVAGLTKISGIFDNR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EIKEAESFRKMLLSVIHDIRVILIKFCDRLHNMRTLDALPPHRQIRIAIETRDIYAPLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECHHHHHHHH
RFGVGRIKIELENLALKYIDREAYDEIVQKLQMTRPERERYLESVMSPIRKKLNELNFEY
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEE
NMSGRPKHVFSIYNKIQQRGKSFEDIYDIYGIRIILHSETNADCYNVYSRITELFSPVPD
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHCCCCH
RFKDFISVPKHNGYASLHTTIVGPGGRMIEVQIRTEKMNEIAETGIAAHWRYKEKTKERD
HHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHCCCHHHEEHHHHHHHHH
KAIDELIKWAREFIENSSMSSTVMDSDSATSFMEGFRMHLYQDEIYVFTPKGDMKTMPVN
HHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHEEECCEEEECCCCCCEECCCC
ATPVDFAFEIHTEVGMRCIGAKVNGKIVSLNSKLKSGDQVEIITSKNQTPKADWEKFVVT
CCCCCEEEEEEHHHCCEEECCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHH
HKAKIKIKQALNEERRTEIEKGRGIWNRVLTKTKSIIALTERDLLKAIKEHGFHFNTPAD
HHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
FYRGLATDKINAEEVLNNLNEKKKSPPQKELTPLTGEAQANTLKKLREPSINQKNKDAVR
HHCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEE
IEGLPGIAFEYARCCNPVPGDDIIGLLSVGSKAVKIHRRNCRSLTSDKLATMKLLDACWE
ECCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDNNGQFLAGLKILGEDVIGMTNKITEVILKSDINIRSISLDASDSLFEGNVLLYVKDVA
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCEEEEEHHHH
TLNRLAEKIKRIKGILSVGRLSNE
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLAQIEEDHYVKLNEILSLCKEKLYHYDEALIRKAFFLCYRAHSEEKRASGEPFFFHPVE
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEECHHH
VAKILLTEIPLDSVSVAAALLHDVVEDTDYTYEDLQDEFGDEIASIVAGLTKISGIFDNR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EIKEAESFRKMLLSVIHDIRVILIKFCDRLHNMRTLDALPPHRQIRIAIETRDIYAPLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECHHHHHHHH
RFGVGRIKIELENLALKYIDREAYDEIVQKLQMTRPERERYLESVMSPIRKKLNELNFEY
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEE
NMSGRPKHVFSIYNKIQQRGKSFEDIYDIYGIRIILHSETNADCYNVYSRITELFSPVPD
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHCCCCH
RFKDFISVPKHNGYASLHTTIVGPGGRMIEVQIRTEKMNEIAETGIAAHWRYKEKTKERD
HHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHCCCHHHEEHHHHHHHHH
KAIDELIKWAREFIENSSMSSTVMDSDSATSFMEGFRMHLYQDEIYVFTPKGDMKTMPVN
HHHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHEEECCEEEECCCCCCEECCCC
ATPVDFAFEIHTEVGMRCIGAKVNGKIVSLNSKLKSGDQVEIITSKNQTPKADWEKFVVT
CCCCCEEEEEEHHHCCEEECCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHH
HKAKIKIKQALNEERRTEIEKGRGIWNRVLTKTKSIIALTERDLLKAIKEHGFHFNTPAD
HHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
FYRGLATDKINAEEVLNNLNEKKKSPPQKELTPLTGEAQANTLKKLREPSINQKNKDAVR
HHCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEE
IEGLPGIAFEYARCCNPVPGDDIIGLLSVGSKAVKIHRRNCRSLTSDKLATMKLLDACWE
ECCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDNNGQFLAGLKILGEDVIGMTNKITEVILKSDINIRSISLDASDSLFEGNVLLYVKDVA
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCCEEEEEHHHH
TLNRLAEKIKRIKGILSVGRLSNE
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]