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The map label for this gene is ygcM [C]

Identifier: 193214205

GI number: 193214205

Start: 568906

End: 569367

Strand: Direct

Name: ygcM [C]

Synonym: Ctha_0486

Alternate gene names: 193214205

Gene position: 568906-569367 (Clockwise)

Preceding gene: 193214204

Following gene: 193214206

Centisome position: 17.27

GC content: 41.13

Gene sequence:

>462_bases
GTGAATACTGAATTCAACCGCATATTGAAAGAAAAACACACGGTTTTTGTAACTCGAAAAGTACATTTTAGTGCCGCTCA
TCGTTTGTTCAATCCTGAATTTTCGGACGCCGAAAATGAGCTGATCTACGATAGATGCAACAACATTTATGGCCATGGAC
ACAATTACGAACTGGAGGTAACGCTAAAAGGCCCGGTTGATAAAGCCACCGGCTACGTGTTTGATTTGAAAAAGCTTGAC
AAAATTCTTTCAGAAGAAATTCTGTCGAAAGTCGATCACAAACATCTCAACTTTGATGTGGAGATGCTAAAAGACATGAT
TCCCTCTGCCGAAGTGCTCTCAATTGTATTCTGGAATGCGATCCAGAACCGCTTAAGTGAATTAGGCTATACGCAAGTCG
CTTTATATGAGGTAAAGCTTTTCGAATCGGAACGGAACTTTGTCCGGTATCGTGGCGATTAA

Upstream 100 bases:

>100_bases
TTTTCGACATAGCCGATTGTTTTTTTATCAATAAAGGATAGGGTTTGTTATTTTCCGCCTGACGCTATAAAAAAAGATTC
TAAAAGCAAATCACGCAGAT

Downstream 100 bases:

>100_bases
TCATCTTGGAACAACTTTGAACATAACTGACATTTAAAACCTTATGAAGCACGCTGAAAAATCCCTTACCGATGCCCGTA
ACGGACATAGAACCGCAACC

Product: hypothetical protein

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; Queuosine Biosynthesis Protein QueD; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MNTEFNRILKEKHTVFVTRKVHFSAAHRLFNPEFSDAENELIYDRCNNIYGHGHNYELEVTLKGPVDKATGYVFDLKKLD
KILSEEILSKVDHKHLNFDVEMLKDMIPSAEVLSIVFWNAIQNRLSELGYTQVALYEVKLFESERNFVRYRGD

Sequences:

>Translated_153_residues
MNTEFNRILKEKHTVFVTRKVHFSAAHRLFNPEFSDAENELIYDRCNNIYGHGHNYELEVTLKGPVDKATGYVFDLKKLD
KILSEEILSKVDHKHLNFDVEMLKDMIPSAEVLSIVFWNAIQNRLSELGYTQVALYEVKLFESERNFVRYRGD
>Mature_153_residues
MNTEFNRILKEKHTVFVTRKVHFSAAHRLFNPEFSDAENELIYDRCNNIYGHGHNYELEVTLKGPVDKATGYVFDLKKLD
KILSEEILSKVDHKHLNFDVEMLKDMIPSAEVLSIVFWNAIQNRLSELGYTQVALYEVKLFESERNFVRYRGD

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=137, Percent_Identity=40.1459854014599, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI115532226, Length=138, Percent_Identity=39.8550724637681, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115532224, Length=138, Percent_Identity=39.8550724637681, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19550074, Length=144, Percent_Identity=38.8888888888889, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24585378, Length=144, Percent_Identity=38.8888888888889, Blast_Score=126, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 17986; Mature: 17986

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00987 PTPS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEFNRILKEKHTVFVTRKVHFSAAHRLFNPEFSDAENELIYDRCNNIYGHGHNYELEV
CCCHHHHHHHHCCEEEEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEE
TLKGPVDKATGYVFDLKKLDKILSEEILSKVDHKHLNFDVEMLKDMIPSAEVLSIVFWNA
EECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
IQNRLSELGYTQVALYEVKLFESERNFVRYRGD
HHHHHHHCCCHHHEEEHEEEECCCCCEEEECCC
>Mature Secondary Structure
MNTEFNRILKEKHTVFVTRKVHFSAAHRLFNPEFSDAENELIYDRCNNIYGHGHNYELEV
CCCHHHHHHHHCCEEEEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEE
TLKGPVDKATGYVFDLKKLDKILSEEILSKVDHKHLNFDVEMLKDMIPSAEVLSIVFWNA
EECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
IQNRLSELGYTQVALYEVKLFESERNFVRYRGD
HHHHHHHCCCHHHEEEHEEEECCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA