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The map label for this gene is entE [H]

Identifier: 193214087

GI number: 193214087

Start: 423706

End: 424983

Strand: Direct

Name: entE [H]

Synonym: Ctha_0368

Alternate gene names: 193214087

Gene position: 423706-424983 (Clockwise)

Preceding gene: 193214086

Following gene: 193214088

Centisome position: 12.87

GC content: 45.07

Gene sequence:

>1278_bases
GTGAATCTACAACCCAATAATCGGTTTATTGTTGACTCGCAAGTCTTTTCCCCTTCAACTTTAGGCGGCAATACCAAAGC
TCTTCTACAACGACATCAATTACACGGCAAGCGTGTTGCAATCATCTCCGATGATATTCATGCTTTGCTGGATGCCATTA
TCTGTTTTTATCAAGAAAAAGCAGATGGCTTAGTCCTTTTCAGAGAAAACATAGAGGACGCATTAAAATCGGTTATAACG
GCTCAAGGCTATACGATTTTATTTCTCGGAAAAAATCAGCAGGAGCTTGTTCAGCCCGCTCAAGCCTCATCTTGCGAAGC
GCGCCCAGGCTATATCTCCATTCTAACTTCGGGGACAACCGGCACGCCGAAACTCATTCCACACACTTGGGATTCGTTAT
TTACATTAAAACACAACACAGGGGTGCAGGCGCTGAGTTGGCTATTTCCTTATACACCAGGGACTTACGCTTGGTATCAG
ATGGTTACCTTATGGATGTTTGTTGAAAATCAAACAATTGTGTCGGCAGCGGAGGTTTTGCCTGAATTGCTATGGGACGT
CGGTGTGCAAAATCAAGTGAATGCCGTGAGTGCAACTCCGACATTTTGGCGATATTTGCTACTACAAAAAGGCACGGATG
AGCTATCAGCATTAAGTATGAAACAGCTTACGCTGGGAGGAGAGCCGATCGACCGAGTGATTTTAAACCAACTGAAAGAA
ATCTATCCGGCAGCAAAACTCACCCATATTTATGCCTCTTCCGAAGCCGGTGCATCTATTGTTGTAAATGACGGCCAAGC
CGGATTTCCCTCTTCTTGGCTCGATGAAAGTGACACCAAAAATAAATCCATCAAGATTGTTGATAACAAATTGTTCATAA
AATCTCCTCATCGCTCCGTTGCCGTCGCAGATTGGTTGGACACGGGAGATGTGGTGGAAATTAAAGATGAGCGCGTGTTT
ATCGTGGGCAGGGAAGGCAGTGAGTATATCAATGTCGGCGGACAGAAAGTCTATTCCAAACTGGTTCAAGATGTCTTGCT
CCAGCATCCAAAAGTCGCGTGGTGCCGGGTCTATGGGAGAAAAGCTCCGATTGTCGGGGAAATGGTTTGCGCCGAAATCG
TCATGAAAAAACAAGTTGCCGGCGAGCCGATAGGTGATTTGGAAAAAGAACTTACGGCGCATTGCAACCGCCATGCGCTT
TCTGCAAACATGATTCCGCGTTTATGGCGGTTCTTAGAGAAAATTCCGGTCGGAAAAAATTTTAAAAGCAAAGTATAA

Upstream 100 bases:

>100_bases
ATCGGAAGGGAATTAAACTTCGATGCTGTCGTTTTGCGAGGAATTCATACCGTAAAAGATGTGTGTAATTTTATTGAAAA
ATCAATTTCTCTAACGAATC

Downstream 100 bases:

>100_bases
AGATGAGCGAACTTCAGAGCGTTATCGTCAGCGGAGGAAGCAGAGGCTTAGGGCTGTCATTCGTAAAACAGTGTTTGGAA
AAAGGCGTAAAGGTCGCAAC

Product: AMP-binding domain-containing protein

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNLQPNNRFIVDSQVFSPSTLGGNTKALLQRHQLHGKRVAIISDDIHALLDAIICFYQEKADGLVLFRENIEDALKSVIT
AQGYTILFLGKNQQELVQPAQASSCEARPGYISILTSGTTGTPKLIPHTWDSLFTLKHNTGVQALSWLFPYTPGTYAWYQ
MVTLWMFVENQTIVSAAEVLPELLWDVGVQNQVNAVSATPTFWRYLLLQKGTDELSALSMKQLTLGGEPIDRVILNQLKE
IYPAAKLTHIYASSEAGASIVVNDGQAGFPSSWLDESDTKNKSIKIVDNKLFIKSPHRSVAVADWLDTGDVVEIKDERVF
IVGREGSEYINVGGQKVYSKLVQDVLLQHPKVAWCRVYGRKAPIVGEMVCAEIVMKKQVAGEPIGDLEKELTAHCNRHAL
SANMIPRLWRFLEKIPVGKNFKSKV

Sequences:

>Translated_425_residues
MNLQPNNRFIVDSQVFSPSTLGGNTKALLQRHQLHGKRVAIISDDIHALLDAIICFYQEKADGLVLFRENIEDALKSVIT
AQGYTILFLGKNQQELVQPAQASSCEARPGYISILTSGTTGTPKLIPHTWDSLFTLKHNTGVQALSWLFPYTPGTYAWYQ
MVTLWMFVENQTIVSAAEVLPELLWDVGVQNQVNAVSATPTFWRYLLLQKGTDELSALSMKQLTLGGEPIDRVILNQLKE
IYPAAKLTHIYASSEAGASIVVNDGQAGFPSSWLDESDTKNKSIKIVDNKLFIKSPHRSVAVADWLDTGDVVEIKDERVF
IVGREGSEYINVGGQKVYSKLVQDVLLQHPKVAWCRVYGRKAPIVGEMVCAEIVMKKQVAGEPIGDLEKELTAHCNRHAL
SANMIPRLWRFLEKIPVGKNFKSKV
>Mature_425_residues
MNLQPNNRFIVDSQVFSPSTLGGNTKALLQRHQLHGKRVAIISDDIHALLDAIICFYQEKADGLVLFRENIEDALKSVIT
AQGYTILFLGKNQQELVQPAQASSCEARPGYISILTSGTTGTPKLIPHTWDSLFTLKHNTGVQALSWLFPYTPGTYAWYQ
MVTLWMFVENQTIVSAAEVLPELLWDVGVQNQVNAVSATPTFWRYLLLQKGTDELSALSMKQLTLGGEPIDRVILNQLKE
IYPAAKLTHIYASSEAGASIVVNDGQAGFPSSWLDESDTKNKSIKIVDNKLFIKSPHRSVAVADWLDTGDVVEIKDERVF
IVGREGSEYINVGGQKVYSKLVQDVLLQHPKVAWCRVYGRKAPIVGEMVCAEIVMKKQVAGEPIGDLEKELTAHCNRHAL
SANMIPRLWRFLEKIPVGKNFKSKV

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786810, Length=298, Percent_Identity=24.496644295302, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 47318; Mature: 47318

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQPNNRFIVDSQVFSPSTLGGNTKALLQRHQLHGKRVAIISDDIHALLDAIICFYQEK
CCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCC
ADGLVLFRENIEDALKSVITAQGYTILFLGKNQQELVQPAQASSCEARPGYISILTSGTT
CCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCCCEEEEEECCCC
GTPKLIPHTWDSLFTLKHNTGVQALSWLFPYTPGTYAWYQMVTLWMFVENQTIVSAAEVL
CCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
PELLWDVGVQNQVNAVSATPTFWRYLLLQKGTDELSALSMKQLTLGGEPIDRVILNQLKE
HHHHHHCCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
IYPAAKLTHIYASSEAGASIVVNDGQAGFPSSWLDESDTKNKSIKIVDNKLFIKSPHRSV
HCCHHHHEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECEEEEECCCCCE
AVADWLDTGDVVEIKDERVFIVGREGSEYINVGGQKVYSKLVQDVLLQHPKVAWCRVYGR
EEEECCCCCCEEEEECCEEEEEECCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECC
KAPIVGEMVCAEIVMKKQVAGEPIGDLEKELTAHCNRHALSANMIPRLWRFLEKIPVGKN
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FKSKV
CCCCC
>Mature Secondary Structure
MNLQPNNRFIVDSQVFSPSTLGGNTKALLQRHQLHGKRVAIISDDIHALLDAIICFYQEK
CCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCC
ADGLVLFRENIEDALKSVITAQGYTILFLGKNQQELVQPAQASSCEARPGYISILTSGTT
CCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCCCEEEEEECCCC
GTPKLIPHTWDSLFTLKHNTGVQALSWLFPYTPGTYAWYQMVTLWMFVENQTIVSAAEVL
CCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
PELLWDVGVQNQVNAVSATPTFWRYLLLQKGTDELSALSMKQLTLGGEPIDRVILNQLKE
HHHHHHCCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
IYPAAKLTHIYASSEAGASIVVNDGQAGFPSSWLDESDTKNKSIKIVDNKLFIKSPHRSV
HCCHHHHEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECEEEEECCCCCE
AVADWLDTGDVVEIKDERVFIVGREGSEYINVGGQKVYSKLVQDVLLQHPKVAWCRVYGR
EEEECCCCCCEEEEECCEEEEEECCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECC
KAPIVGEMVCAEIVMKKQVAGEPIGDLEKELTAHCNRHALSANMIPRLWRFLEKIPVGKN
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FKSKV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]