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The map label for this gene is pleD [H]

Identifier: 193214078

GI number: 193214078

Start: 412740

End: 414560

Strand: Direct

Name: pleD [H]

Synonym: Ctha_0359

Alternate gene names: 193214078

Gene position: 412740-414560 (Clockwise)

Preceding gene: 193214077

Following gene: 193214080

Centisome position: 12.53

GC content: 44.92

Gene sequence:

>1821_bases
ATGCCAAACGGAATTCAAGAACTGGAGAGCAAACTTCAACAGGCCACAAACGATGAGGAAAAAATAGAACTGCTCAGTGA
ACTTGCCTGGCACTTGGTCAATATCAACCCAAAGCGAGGCGGAGAACTTAGCCAAGAAGCCTATTTATTAGCCCAAACCA
TTGGCTATACTTACGGCATTGCCAAAAGCCTTCGTGCGCAGGCGGCTTGCCTTTCGTTTCTTTCTCAATATCGTGCCGCG
TTGGAAAAAGCAGAGCTAGCCGTTGGCCTATTTCGGCAGCTTAAGGACACGCCCGGCTTGGCACTGGCCAAAATCATTTT
GGCCGAATGCGATACAAAACTGGGCTTGTTCAAAGAAGCCATAGAAATGTATACAAAAGCCTATAAGCTTTATGAAATAC
TCGGCGAAAGATGTTCCCAAACGACCATTTTAACCAGCATGGGAAAAATTTTTCAAAATATCGGCGATTATGAAAAAGCG
GTGGATTATCACTTAAAAGCGCTTTCGCTTGATCCCGATAAAGACAGCCTAAATACAGCAGGCGTTTTATCAAATTTAGC
TGATGTGTATTTTGCGTTAGGAAAGTATAAAACCAGCCTGGACTATCTCACTCGCGCGATTCATATCTATAAATTACAAG
AGCACTTATATGGCATTACCAAATGTTTAGGCAAAATTGGTGATATTTACCTAACCCTTAACCAACTCGATAAAGCAGAA
GAATTTTACACCAAAAGTTTCGGCTGCTCCTACAACTTCCAGGATAAAGAAGGCCAAGCCTCGTGCTTGATTAGCTTTGG
CAAGCTTTATATCCAAAAATGCAATTATGAAAAAGCTGAAAAAAGCTTTCGGGCTGGCTATGAAGCTGCGCAAAAAATAT
CCCACAAACACTTTATGGCAGAAGCCAAGCTGGGCTTGGGGCGCGTTCGGAAAATGGAGGAAAAATGGGACGAGGCGATC
GAACATTTTAACTACGCCTTGACTTTGGGCAAGGAATTGAATTTGCACAACATCGTTTTTGAATCGCATCGATATTTGTG
CCTGGTGTTTGAAAAATTGGAGGACTACAAAGCAGCGCTTGAGCATCACAAGCTTTTTTATGCCGTTAAAACCAAAATTT
TCAGCGAAGAAGCTGAGCGAAAAACCAAAAATCTTTTGCTTCAGCACGAACTGGAAGAGTTCAAACGAGAAGCTGAAATT
TATCGGCTAAAAAATGTTGAACTCGTTTCCGCCAATGAAAATCTGGAAGTCACCACGCTGGCACTCAAAGAAGCCAATGC
GGAAAATCAACTTTTGCTGGAACGCTTGCGCGAACAAGCCGAAACGCTGGAGCGGCTTGTCACCTACGATGGCCTCACCG
GACTTGCAAATCGCCGCCATTTTGACGAGCAGTTCGAGTATGAATTTCGCCGCACACGCCGTTATAAACGGCCTCTTTCA
ATCGCGATGTCGGATATTGATTTTTTCAAAAAAGTCAACGACAACTTCTCGCACCAAGTTGGCGATGAAGTGCTCAAAAA
AGTTGCCAGCATTTTTAGAGAAAATGTGCGCGCAGGCGATTTGGTGGCGCGCTACGGCGGCGAAGAGTTCGTGTTTATTT
TTACCGAAACCAATAAAGAAAGCGCCATTCGAGCGGCTGAAAAAATTCGCAAAGCCATTGAAGACTACGACTGGAGCAAC
GTCCATGCCGGATTGAAAGTGACCATTAGCATAGGCGTTGCTGAGGACGCGGGCTTCGACGGCTGCGATGAACTGATTGC
CGCCGCCGATAGCAAACTCTACGAGGCCAAGCGCAATGGCCGAAATCAAGTGCGCGCTTAA

Upstream 100 bases:

>100_bases
CATATTTACATTCATTAACCCTAACACCTCAATCGATTAAGCAATTAGACGACTGAGAGAACGTTTCATTCTTTCATTAA
TGCAGTGAAGTCTATCATTT

Downstream 100 bases:

>100_bases
CGCTTATCTGATTCTTGTTCGTGACAATAACGCGCGAAAAATTCAGCCTTCCAAGTTTGAAAATCCCCCTTTAAAATATG
TGCTCTGGCTGTTTTGGTTA

Product: diguanylate cyclase/serine/threonine protein kinase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MPNGIQELESKLQQATNDEEKIELLSELAWHLVNINPKRGGELSQEAYLLAQTIGYTYGIAKSLRAQAACLSFLSQYRAA
LEKAELAVGLFRQLKDTPGLALAKIILAECDTKLGLFKEAIEMYTKAYKLYEILGERCSQTTILTSMGKIFQNIGDYEKA
VDYHLKALSLDPDKDSLNTAGVLSNLADVYFALGKYKTSLDYLTRAIHIYKLQEHLYGITKCLGKIGDIYLTLNQLDKAE
EFYTKSFGCSYNFQDKEGQASCLISFGKLYIQKCNYEKAEKSFRAGYEAAQKISHKHFMAEAKLGLGRVRKMEEKWDEAI
EHFNYALTLGKELNLHNIVFESHRYLCLVFEKLEDYKAALEHHKLFYAVKTKIFSEEAERKTKNLLLQHELEEFKREAEI
YRLKNVELVSANENLEVTTLALKEANAENQLLLERLREQAETLERLVTYDGLTGLANRRHFDEQFEYEFRRTRRYKRPLS
IAMSDIDFFKKVNDNFSHQVGDEVLKKVASIFRENVRAGDLVARYGGEEFVFIFTETNKESAIRAAEKIRKAIEDYDWSN
VHAGLKVTISIGVAEDAGFDGCDELIAAADSKLYEAKRNGRNQVRA

Sequences:

>Translated_606_residues
MPNGIQELESKLQQATNDEEKIELLSELAWHLVNINPKRGGELSQEAYLLAQTIGYTYGIAKSLRAQAACLSFLSQYRAA
LEKAELAVGLFRQLKDTPGLALAKIILAECDTKLGLFKEAIEMYTKAYKLYEILGERCSQTTILTSMGKIFQNIGDYEKA
VDYHLKALSLDPDKDSLNTAGVLSNLADVYFALGKYKTSLDYLTRAIHIYKLQEHLYGITKCLGKIGDIYLTLNQLDKAE
EFYTKSFGCSYNFQDKEGQASCLISFGKLYIQKCNYEKAEKSFRAGYEAAQKISHKHFMAEAKLGLGRVRKMEEKWDEAI
EHFNYALTLGKELNLHNIVFESHRYLCLVFEKLEDYKAALEHHKLFYAVKTKIFSEEAERKTKNLLLQHELEEFKREAEI
YRLKNVELVSANENLEVTTLALKEANAENQLLLERLREQAETLERLVTYDGLTGLANRRHFDEQFEYEFRRTRRYKRPLS
IAMSDIDFFKKVNDNFSHQVGDEVLKKVASIFRENVRAGDLVARYGGEEFVFIFTETNKESAIRAAEKIRKAIEDYDWSN
VHAGLKVTISIGVAEDAGFDGCDELIAAADSKLYEAKRNGRNQVRA
>Mature_605_residues
PNGIQELESKLQQATNDEEKIELLSELAWHLVNINPKRGGELSQEAYLLAQTIGYTYGIAKSLRAQAACLSFLSQYRAAL
EKAELAVGLFRQLKDTPGLALAKIILAECDTKLGLFKEAIEMYTKAYKLYEILGERCSQTTILTSMGKIFQNIGDYEKAV
DYHLKALSLDPDKDSLNTAGVLSNLADVYFALGKYKTSLDYLTRAIHIYKLQEHLYGITKCLGKIGDIYLTLNQLDKAEE
FYTKSFGCSYNFQDKEGQASCLISFGKLYIQKCNYEKAEKSFRAGYEAAQKISHKHFMAEAKLGLGRVRKMEEKWDEAIE
HFNYALTLGKELNLHNIVFESHRYLCLVFEKLEDYKAALEHHKLFYAVKTKIFSEEAERKTKNLLLQHELEEFKREAEIY
RLKNVELVSANENLEVTTLALKEANAENQLLLERLREQAETLERLVTYDGLTGLANRRHFDEQFEYEFRRTRRYKRPLSI
AMSDIDFFKKVNDNFSHQVGDEVLKKVASIFRENVRAGDLVARYGGEEFVFIFTETNKESAIRAAEKIRKAIEDYDWSNV
HAGLKVTISIGVAEDAGFDGCDELIAAADSKLYEAKRNGRNQVRA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Homo sapiens, GI224028289, Length=333, Percent_Identity=20.7207207207207, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI145693134, Length=195, Percent_Identity=39.4871794871795, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=34.7058823529412, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=39.3063583815029, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=40.8805031446541, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1787816, Length=154, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788381, Length=196, Percent_Identity=31.6326530612245, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=34.375, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788085, Length=176, Percent_Identity=32.9545454545455, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=32.0512820512821, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=30.635838150289, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=26.7857142857143, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=150, Percent_Identity=25.3333333333333, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 69337; Mature: 69206

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50005 TPR L=RR ; PS50293 TPR_REGION ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNGIQELESKLQQATNDEEKIELLSELAWHLVNINPKRGGELSQEAYLLAQTIGYTYGI
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
AKSLRAQAACLSFLSQYRAALEKAELAVGLFRQLKDTPGLALAKIILAECDTKLGLFKEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHH
IEMYTKAYKLYEILGERCSQTTILTSMGKIFQNIGDYEKAVDYHLKALSLDPDKDSLNTA
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHEEECCCCCCCCHHH
GVLSNLADVYFALGKYKTSLDYLTRAIHIYKLQEHLYGITKCLGKIGDIYLTLNQLDKAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHH
EFYTKSFGCSYNFQDKEGQASCLISFGKLYIQKCNYEKAEKSFRAGYEAAQKISHKHFMA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EAKLGLGRVRKMEEKWDEAIEHFNYALTLGKELNLHNIVFESHRYLCLVFEKLEDYKAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
EHHKLFYAVKTKIFSEEAERKTKNLLLQHELEEFKREAEIYRLKNVELVSANENLEVTTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEE
ALKEANAENQLLLERLREQAETLERLVTYDGLTGLANRRHFDEQFEYEFRRTRRYKRPLS
EEHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCHH
IAMSDIDFFKKVNDNFSHQVGDEVLKKVASIFRENVRAGDLVARYGGEEFVFIFTETNKE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEEECCHH
SAIRAAEKIRKAIEDYDWSNVHAGLKVTISIGVAEDAGFDGCDELIAAADSKLYEAKRNG
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
RNQVRA
CCCCCC
>Mature Secondary Structure 
PNGIQELESKLQQATNDEEKIELLSELAWHLVNINPKRGGELSQEAYLLAQTIGYTYGI
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH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CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA