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The map label for this gene is asnB [H]

Identifier: 193214080

GI number: 193214080

Start: 415873

End: 417798

Strand: Direct

Name: asnB [H]

Synonym: Ctha_0361

Alternate gene names: 193214080

Gene position: 415873-417798 (Clockwise)

Preceding gene: 193214078

Following gene: 193214081

Centisome position: 12.63

GC content: 48.39

Gene sequence:

>1926_bases
ATGTGTGGGATTGCCGGCATCATTACTGGTGGAAACCCGGAGATTTTGCAAAAAATGGTGGACATTCAGGCGCATCGCGG
CCCTGACGACGCCGGCTTGCAATGGTTCGAATCTGCTAACGCCGGACTCGCACAACGCCGGCTTTCCATTTTAGATTTGA
CGAACGCTGGGCATCAGCCCATGAAAAATGCTCGTGGGAACCGCTGGATTACCTTCAACGGCGAAATTTATAACTACCGC
GAACTCCGCGCAGAACTGCAAACCAAAGGCTATCAGTTCAAATCCAATTCCGATACGGAAGTCATTTTAGCCGCCTACGA
CGAATGGGGCGCTTCAGCTGTGAATCGTTTCAACGGCATGTTTGCTTTTGCGATTTACGACACCGATACACGCGAGCTTT
TTTTAGCAAGAGATCATTTGGGCATCAAACCGCTTTATTACGCCCAACTTGGCGAAACGCTTCTTTTTGCCTCCGAAGCA
AAAGCGCTTTTCGAATATCCGGCGATGAAAAAAGAAATCGAACCCGACGCGGTTGTGAGCGCAATCATGCTGCTTTGGGT
GCCGGAGCCGAAAACGGGCTTCAAGAATGTCTTCAAACTGCCAGCCGGACATTACGCGATTTTTAAAAATGGCAAGCTTT
CTATTCAGGAATATTGGGACATACCGATTCCTGAAACCACGCCAACCTTAACGCCTGCGTTGGAAGCCGAAAGCACAGCC
AAACTGGTTGAATTGCTGGAGCAAACCATCGCGCGGCAAATGATTTCGGATGTGCCGGTAGGCGCGTTTTTGTCGGGCGG
ACTGGATTCATCGCTTATTGTCGCGCTCATGCGCAAGGCGGCGCCCGGCGCGAAGCTTTCGACTTACACCATCGCCTTTA
CTGAAGCCGATAAAAAAATGGAGGCCATGCCCGACGACGCTATGTATGCTCGGCGCGTCGCCAAAGAATTCGGCACGGAG
CATCACGAGCTTTGCATCGAGCCGGATAGCAATAAGCATTTGCAAAAAATCCTCTGGCATTTGGACGATCCCGTCGCCGA
TGGCGCGGCCATCAACACGTATTTGATTTCCAAACAAGCAAAAGACAACGGCACAACCGTGCTTCTGAATGGCATGGGCG
GCGACGAAGTTTTTGGCGGCTATCGCAAACACTTTGCCTCATTAATGATTGAGCGTTATCAAAAAATCCCGACGCCGCTT
CGCAAAGGCCTGATTGAACCGACAGTCAACGCCATGCCGGTGGCAATTGGCGGCAAGGGCATTATTCTAACGCGCTGGGC
AAAACGCTTCCTTGGTAGCGCAAGCATATCACCATTAAACGCTTATATTTATGGATTTAATTATCATAAACCTGATGGCT
TAAAACAAATTCTCGCCGAGCATTTGTTCCAAACGGATTTTGAAAATCTTTATCCAGTTCGCCGCTATTTTGAACTGGCA
GAACGCGTCAAGCATTTGCCGCTTCTGCAAAAAATGACTTATCTCGATACGAAGCTTTTTTTAACCGGCATCAATCTGCT
TTATTCCGACAAAGCGATGATGGCCGCTTCGGTGGAAGGTCGCCCGCCGCTGATTGATAAAGAAATTGTAGAATTTGCTG
CTTATTTACCGTCGGAGCTAAAAATCAAAGGCCGCGAACAAAAATATCTCTTAAAGAAAGCTGCTGAATCCTACCTCCCT
AACGACATCATTTATAGACCAAAAGCCCCTTTTGGCACGCCGCTTCGTGCATGGATGAAAACCGGTTTGAAATCGCAAAT
GGAAACCTTATTTTCCAATAACGATTTTCCCCATAATCACTATCTCCAAAATGCTTACGTACTGAATTTGCTGGAAGCTC
ATGCAAGCGGGAAAACCGACAACGCACACGCACTTTGGGGCGCTTTTGGCGTGGCAAATTGGATTAACCAAAATGAACCT
GCTTAG

Upstream 100 bases:

>100_bases
AAAGAGTTTAAAATACTTATATTCAACGGCAACAAAAAGCTGGAACAAGCGAGAAAATTTTCTTTTCAGAAACATTCATT
TGTAGAAAGAGCGCGTTACT

Downstream 100 bases:

>100_bases
CTTGATTTTTTTCAAAGCTTTTCACGTAATTGATTAAAAAAACATGGCTACTTCGAAAGAAAGCATTGAAAAAATTCAGA
ACGCTGTTCTCCAAACAGAA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MCGIAGIITGGNPEILQKMVDIQAHRGPDDAGLQWFESANAGLAQRRLSILDLTNAGHQPMKNARGNRWITFNGEIYNYR
ELRAELQTKGYQFKSNSDTEVILAAYDEWGASAVNRFNGMFAFAIYDTDTRELFLARDHLGIKPLYYAQLGETLLFASEA
KALFEYPAMKKEIEPDAVVSAIMLLWVPEPKTGFKNVFKLPAGHYAIFKNGKLSIQEYWDIPIPETTPTLTPALEAESTA
KLVELLEQTIARQMISDVPVGAFLSGGLDSSLIVALMRKAAPGAKLSTYTIAFTEADKKMEAMPDDAMYARRVAKEFGTE
HHELCIEPDSNKHLQKILWHLDDPVADGAAINTYLISKQAKDNGTTVLLNGMGGDEVFGGYRKHFASLMIERYQKIPTPL
RKGLIEPTVNAMPVAIGGKGIILTRWAKRFLGSASISPLNAYIYGFNYHKPDGLKQILAEHLFQTDFENLYPVRRYFELA
ERVKHLPLLQKMTYLDTKLFLTGINLLYSDKAMMAASVEGRPPLIDKEIVEFAAYLPSELKIKGREQKYLLKKAAESYLP
NDIIYRPKAPFGTPLRAWMKTGLKSQMETLFSNNDFPHNHYLQNAYVLNLLEAHASGKTDNAHALWGAFGVANWINQNEP
A

Sequences:

>Translated_641_residues
MCGIAGIITGGNPEILQKMVDIQAHRGPDDAGLQWFESANAGLAQRRLSILDLTNAGHQPMKNARGNRWITFNGEIYNYR
ELRAELQTKGYQFKSNSDTEVILAAYDEWGASAVNRFNGMFAFAIYDTDTRELFLARDHLGIKPLYYAQLGETLLFASEA
KALFEYPAMKKEIEPDAVVSAIMLLWVPEPKTGFKNVFKLPAGHYAIFKNGKLSIQEYWDIPIPETTPTLTPALEAESTA
KLVELLEQTIARQMISDVPVGAFLSGGLDSSLIVALMRKAAPGAKLSTYTIAFTEADKKMEAMPDDAMYARRVAKEFGTE
HHELCIEPDSNKHLQKILWHLDDPVADGAAINTYLISKQAKDNGTTVLLNGMGGDEVFGGYRKHFASLMIERYQKIPTPL
RKGLIEPTVNAMPVAIGGKGIILTRWAKRFLGSASISPLNAYIYGFNYHKPDGLKQILAEHLFQTDFENLYPVRRYFELA
ERVKHLPLLQKMTYLDTKLFLTGINLLYSDKAMMAASVEGRPPLIDKEIVEFAAYLPSELKIKGREQKYLLKKAAESYLP
NDIIYRPKAPFGTPLRAWMKTGLKSQMETLFSNNDFPHNHYLQNAYVLNLLEAHASGKTDNAHALWGAFGVANWINQNEP
A
>Mature_641_residues
MCGIAGIITGGNPEILQKMVDIQAHRGPDDAGLQWFESANAGLAQRRLSILDLTNAGHQPMKNARGNRWITFNGEIYNYR
ELRAELQTKGYQFKSNSDTEVILAAYDEWGASAVNRFNGMFAFAIYDTDTRELFLARDHLGIKPLYYAQLGETLLFASEA
KALFEYPAMKKEIEPDAVVSAIMLLWVPEPKTGFKNVFKLPAGHYAIFKNGKLSIQEYWDIPIPETTPTLTPALEAESTA
KLVELLEQTIARQMISDVPVGAFLSGGLDSSLIVALMRKAAPGAKLSTYTIAFTEADKKMEAMPDDAMYARRVAKEFGTE
HHELCIEPDSNKHLQKILWHLDDPVADGAAINTYLISKQAKDNGTTVLLNGMGGDEVFGGYRKHFASLMIERYQKIPTPL
RKGLIEPTVNAMPVAIGGKGIILTRWAKRFLGSASISPLNAYIYGFNYHKPDGLKQILAEHLFQTDFENLYPVRRYFELA
ERVKHLPLLQKMTYLDTKLFLTGINLLYSDKAMMAASVEGRPPLIDKEIVEFAAYLPSELKIKGREQKYLLKKAAESYLP
NDIIYRPKAPFGTPLRAWMKTGLKSQMETLFSNNDFPHNHYLQNAYVLNLLEAHASGKTDNAHALWGAFGVANWINQNEP
A

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=407, Percent_Identity=28.5012285012285, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI168229252, Length=407, Percent_Identity=28.5012285012285, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI168229250, Length=407, Percent_Identity=28.5012285012285, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI296010848, Length=383, Percent_Identity=28.9817232375979, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI296010852, Length=314, Percent_Identity=28.9808917197452, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI296010850, Length=314, Percent_Identity=28.9808917197452, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1786889, Length=407, Percent_Identity=34.1523341523341, Blast_Score=182, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI71993933, Length=409, Percent_Identity=28.8508557457213, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI25147557, Length=395, Percent_Identity=29.1139240506329, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI25147560, Length=336, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321563, Length=417, Percent_Identity=29.0167865707434, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325403, Length=418, Percent_Identity=28.9473684210526, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45553209, Length=404, Percent_Identity=30.9405940594059, Blast_Score=160, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71780; Mature: 71780

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIITGGNPEILQKMVDIQAHRGPDDAGLQWFESANAGLAQRRLSILDLTNAGHQP
CCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHEEECCCCCCC
MKNARGNRWITFNGEIYNYRELRAELQTKGYQFKSNSDTEVILAAYDEWGASAVNRFNGM
HHHCCCCEEEEECCCEECHHHHHHHHHHCCEEECCCCCCEEEEEECCHHCHHHHHHCCCE
FAFAIYDTDTRELFLARDHLGIKPLYYAQLGETLLFASEAKALFEYPAMKKEIEPDAVVS
EEEEEEECCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHCCCHHHHHH
AIMLLWVPEPKTGFKNVFKLPAGHYAIFKNGKLSIQEYWDIPIPETTPTLTPALEAESTA
HHHHHCCCCCCCCHHHHHHCCCCCEEEEECCCEEEHEECCCCCCCCCCCCCCCCCCHHHH
KLVELLEQTIARQMISDVPVGAFLSGGLDSSLIVALMRKAAPGAKLSTYTIAFTEADKKM
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCHHH
EAMPDDAMYARRVAKEFGTEHHELCIEPDSNKHLQKILWHLDDPVADGAAINTYLISKQA
HCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCEEEHEEEECCC
KDNGTTVLLNGMGGDEVFGGYRKHFASLMIERYQKIPTPLRKGLIEPTVNAMPVAIGGKG
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEECCCC
IILTRWAKRFLGSASISPLNAYIYGFNYHKPDGLKQILAEHLFQTDFENLYPVRRYFELA
EEHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERVKHLPLLQKMTYLDTKLFLTGINLLYSDKAMMAASVEGRPPLIDKEIVEFAAYLPSEL
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCHHHHHHHHHHCCCCE
KIKGREQKYLLKKAAESYLPNDIIYRPKAPFGTPLRAWMKTGLKSQMETLFSNNDFPHNH
EECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
YLQNAYVLNLLEAHASGKTDNAHALWGAFGVANWINQNEPA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCGIAGIITGGNPEILQKMVDIQAHRGPDDAGLQWFESANAGLAQRRLSILDLTNAGHQP
CCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHEEECCCCCCC
MKNARGNRWITFNGEIYNYRELRAELQTKGYQFKSNSDTEVILAAYDEWGASAVNRFNGM
HHHCCCCEEEEECCCEECHHHHHHHHHHCCEEECCCCCCEEEEEECCHHCHHHHHHCCCE
FAFAIYDTDTRELFLARDHLGIKPLYYAQLGETLLFASEAKALFEYPAMKKEIEPDAVVS
EEEEEEECCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHCCCHHHHHH
AIMLLWVPEPKTGFKNVFKLPAGHYAIFKNGKLSIQEYWDIPIPETTPTLTPALEAESTA
HHHHHCCCCCCCCHHHHHHCCCCCEEEEECCCEEEHEECCCCCCCCCCCCCCCCCCHHHH
KLVELLEQTIARQMISDVPVGAFLSGGLDSSLIVALMRKAAPGAKLSTYTIAFTEADKKM
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCHHH
EAMPDDAMYARRVAKEFGTEHHELCIEPDSNKHLQKILWHLDDPVADGAAINTYLISKQA
HCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCEEEHEEEECCC
KDNGTTVLLNGMGGDEVFGGYRKHFASLMIERYQKIPTPLRKGLIEPTVNAMPVAIGGKG
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEECCCC
IILTRWAKRFLGSASISPLNAYIYGFNYHKPDGLKQILAEHLFQTDFENLYPVRRYFELA
EEHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERVKHLPLLQKMTYLDTKLFLTGINLLYSDKAMMAASVEGRPPLIDKEIVEFAAYLPSEL
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCHHHHHHHHHHCCCCE
KIKGREQKYLLKKAAESYLPNDIIYRPKAPFGTPLRAWMKTGLKSQMETLFSNNDFPHNH
EECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHH
YLQNAYVLNLLEAHASGKTDNAHALWGAFGVANWINQNEPA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]