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The map label for this gene is pcm [H]

Identifier: 192291553

GI number: 192291553

Start: 3426252

End: 3426905

Strand: Reverse

Name: pcm [H]

Synonym: Rpal_3179

Alternate gene names: 192291553

Gene position: 3426905-3426252 (Counterclockwise)

Preceding gene: 192291555

Following gene: 192291552

Centisome position: 59.66

GC content: 70.03

Gene sequence:

>654_bases
ATGCCGAGCCCCGCGGCGCCGCCGCCCGAAAAGATGATGTTCCAGCTCAGCCTGCGGCGGCGCGGCATCAGCGATCAGGC
GGTGCTGCGGACGATGGAGGCGGTGCCGCGCGACCAGTTCGTCGACCCCAATTATCGCGACGGCGCCTGGCGCGACACCG
CGCTGCCGATCGCCTGCGGCCAGACCATCAGTCAGCCGTTCGTGGTCGCCTTCATGACCGAGCAGCTCGAGCTGCGTCCG
CGCGACCGCGTGCTCGAGATCGGCACCGGCTCCGGCTACCACGCGGCGGTGCTGTCGCGGCTGGCGGCGGACGTGCTCAG
CTTCGAGCGCTTCAAGACGCTGGCCGACCGCGCCCGCAAACGACTCGCCGAGCTGGGGTGCAGCAATGTCGAGGTGGTGT
TCGGAGACGGATTCGATCTGCCGGAGACCGCCGGCACATTTGATCGCATCCTGATCACTGCCGCCGTCCCGGAGCTGCCC
GCGCGGCTGCTCGAGCGACTCGATCCTGGCGGTGTTCTGATCGCACCGGTCGGGCCGCCGAACGGTCGGCAGACACTGCT
GCGTGTGCGCCGCACGCCGAACGGCGACGAGCACAAGGCCCTGGGGGAGGTCCGTTTCGTGCCCGCGCTCCGCGGCATCG
CACGCGAGCTTTGA

Upstream 100 bases:

>100_bases
CGCACTTGCAAGGTTTCCTTCGGCACGCCAACACTCTGCTGAGAATCGCGCCAATTGGAACCCGACCGGGAAAGGCGCAC
GACCGATCGCGAGACCCGAA

Downstream 100 bases:

>100_bases
GCTAGCTGGATTTTTCGATTTCTCAGCGTCAACAAGCGGTTTCTTTGCAAGGTTAAACGCTTGTTTACTGGCGCGGTGTT
TACTCAATTTACCAATCGTA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MPSPAAPPPEKMMFQLSLRRRGISDQAVLRTMEAVPRDQFVDPNYRDGAWRDTALPIACGQTISQPFVVAFMTEQLELRP
RDRVLEIGTGSGYHAAVLSRLAADVLSFERFKTLADRARKRLAELGCSNVEVVFGDGFDLPETAGTFDRILITAAVPELP
ARLLERLDPGGVLIAPVGPPNGRQTLLRVRRTPNGDEHKALGEVRFVPALRGIAREL

Sequences:

>Translated_217_residues
MPSPAAPPPEKMMFQLSLRRRGISDQAVLRTMEAVPRDQFVDPNYRDGAWRDTALPIACGQTISQPFVVAFMTEQLELRP
RDRVLEIGTGSGYHAAVLSRLAADVLSFERFKTLADRARKRLAELGCSNVEVVFGDGFDLPETAGTFDRILITAAVPELP
ARLLERLDPGGVLIAPVGPPNGRQTLLRVRRTPNGDEHKALGEVRFVPALRGIAREL
>Mature_216_residues
PSPAAPPPEKMMFQLSLRRRGISDQAVLRTMEAVPRDQFVDPNYRDGAWRDTALPIACGQTISQPFVVAFMTEQLELRPR
DRVLEIGTGSGYHAAVLSRLAADVLSFERFKTLADRARKRLAELGCSNVEVVFGDGFDLPETAGTFDRILITAAVPELPA
RLLERLDPGGVLIAPVGPPNGRQTLLRVRRTPNGDEHKALGEVRFVPALRGIAREL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=204, Percent_Identity=30.3921568627451, Blast_Score=77, Evalue=8e-15,
Organism=Escherichia coli, GI1789100, Length=196, Percent_Identity=48.9795918367347, Blast_Score=177, Evalue=6e-46,
Organism=Drosophila melanogaster, GI17981723, Length=202, Percent_Identity=31.6831683168317, Blast_Score=80, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23892; Mature: 23761

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPAAPPPEKMMFQLSLRRRGISDQAVLRTMEAVPRDQFVDPNYRDGAWRDTALPIACG
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCC
QTISQPFVVAFMTEQLELRPRDRVLEIGTGSGYHAAVLSRLAADVLSFERFKTLADRARK
CCCCCCEEEEEEHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAELGCSNVEVVFGDGFDLPETAGTFDRILITAAVPELPARLLERLDPGGVLIAPVGPP
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEECCCC
NGRQTLLRVRRTPNGDEHKALGEVRFVPALRGIAREL
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSPAAPPPEKMMFQLSLRRRGISDQAVLRTMEAVPRDQFVDPNYRDGAWRDTALPIACG
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCC
QTISQPFVVAFMTEQLELRPRDRVLEIGTGSGYHAAVLSRLAADVLSFERFKTLADRARK
CCCCCCEEEEEEHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAELGCSNVEVVFGDGFDLPETAGTFDRILITAAVPELPARLLERLDPGGVLIAPVGPP
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEECCCC
NGRQTLLRVRRTPNGDEHKALGEVRFVPALRGIAREL
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA