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The map label for this gene is hemB [H]

Identifier: 192291366

GI number: 192291366

Start: 3258666

End: 3259718

Strand: Reverse

Name: hemB [H]

Synonym: Rpal_2988

Alternate gene names: 192291366

Gene position: 3259718-3258666 (Counterclockwise)

Preceding gene: 192291374

Following gene: 192291365

Centisome position: 56.75

GC content: 67.14

Gene sequence:

>1053_bases
ATGGCGATCAAATTCGGCCGTCCCATCGAGATTCGTGACCCCTCGACCCGGCCCGCCGCTCCGCCGCTCGATCTCACCAT
CCGTCCGCGCCGCAACCGCAAGACCGAGTGGGCTCGCCGTCTGGTGCGCGAGAATGTCCTCACCACCGACGACCTGATCT
GGCCGATGTTCGTGGTCGACGGCACCAACCAGCGGGTTCAGGTCGCCTCGATGCCGGGCGTCGAACGACTCAGCGTCGAC
CAGATCGTTCGCGACGCCGAACGCGCCGCCAAGCTCGACATCCCGTGCATCGCGCTGTTCCCATTCACCGAGCCGTCGCT
GCGCGACGAGCACGGCACCGAAGCCGCCAACCCGGAAAACCTGATCTGCAAGACCGTCCGCGCGATCAAGAAGGAATTCC
CGCAGATCGGCGTGCTGTGCGACGTCGCGCTCGACCCCTACACCAGCCACGGCCATGACGGCCTGATCGAGGACGGCCGC
ATCCTCAACGACGAGACCGTCGCGGTGCTGGTGCAGCAGTCGCTGGTGCAGGCGCAGGCCGGCTGCGACGTGATCGCACC
GTCGGACATGATGGATGGCCGCATCGGCGCGATCCGCGAAGCGCTCGATGCCGCCGGCTACCTCGACGTTCAGATCATGG
CCTATGCGGCCAAGTATGCGTCCGCCTTCTACGGCCCGTTCCGCGACGCGATCGGCTCGGCCAAGGCGCTCACCGGCGAC
AAGCGCACCTACCAGATGGATCCGGCCAACACCGACGAGGCGATCCGCGAGGTCGAGCTCGATCTCGCCGAGGGCGCCGA
CATGGTCATGGTCAAGCCGGGCATGCCGTATCTCGACATCGTCCGCCGCGTGAAGGAGACCTTCGGCGTGCCGACCTTCG
CCTATCAGGTGTCGGGCGAGTACGCGATGATCGCCGCGGCAGCCAACAATGGCTGGCTGGACGGCGACCGGGCGATGATG
GAGAGCCTGCTCGCCTTCAAGCGCGCCGGCGCCAACGGCGTGCTGACCTACTTCGCCCCGGCGGCGGCCGAAAAGCTGCG
GCAGCAGGGCTAA

Upstream 100 bases:

>100_bases
GGAACACTCTGATTGCGCCAAATCAACAGTGCGGTGACGCGGGGGTGCCAAAATGCTACTTTCCATGAACACAGCAGAGC
CTGCAGGGAGAGTTGGCACC

Downstream 100 bases:

>100_bases
ATCTCCCTCCTCGCTTGTTTCAACCCTCCATCGATTCGCCGGTCTCCGGCGGATCAATGGAGGGTTTGTAATGTCGCGCC
AGCCTTGCAAGGCGCCGCTG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAIKFGRPIEIRDPSTRPAAPPLDLTIRPRRNRKTEWARRLVRENVLTTDDLIWPMFVVDGTNQRVQVASMPGVERLSVD
QIVRDAERAAKLDIPCIALFPFTEPSLRDEHGTEAANPENLICKTVRAIKKEFPQIGVLCDVALDPYTSHGHDGLIEDGR
ILNDETVAVLVQQSLVQAQAGCDVIAPSDMMDGRIGAIREALDAAGYLDVQIMAYAAKYASAFYGPFRDAIGSAKALTGD
KRTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFGVPTFAYQVSGEYAMIAAAANNGWLDGDRAMM
ESLLAFKRAGANGVLTYFAPAAAEKLRQQG

Sequences:

>Translated_350_residues
MAIKFGRPIEIRDPSTRPAAPPLDLTIRPRRNRKTEWARRLVRENVLTTDDLIWPMFVVDGTNQRVQVASMPGVERLSVD
QIVRDAERAAKLDIPCIALFPFTEPSLRDEHGTEAANPENLICKTVRAIKKEFPQIGVLCDVALDPYTSHGHDGLIEDGR
ILNDETVAVLVQQSLVQAQAGCDVIAPSDMMDGRIGAIREALDAAGYLDVQIMAYAAKYASAFYGPFRDAIGSAKALTGD
KRTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFGVPTFAYQVSGEYAMIAAAANNGWLDGDRAMM
ESLLAFKRAGANGVLTYFAPAAAEKLRQQG
>Mature_349_residues
AIKFGRPIEIRDPSTRPAAPPLDLTIRPRRNRKTEWARRLVRENVLTTDDLIWPMFVVDGTNQRVQVASMPGVERLSVDQ
IVRDAERAAKLDIPCIALFPFTEPSLRDEHGTEAANPENLICKTVRAIKKEFPQIGVLCDVALDPYTSHGHDGLIEDGRI
LNDETVAVLVQQSLVQAQAGCDVIAPSDMMDGRIGAIREALDAAGYLDVQIMAYAAKYASAFYGPFRDAIGSAKALTGDK
RTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFGVPTFAYQVSGEYAMIAAAANNGWLDGDRAMME
SLLAFKRAGANGVLTYFAPAAAEKLRQQG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=43.1818181818182, Blast_Score=248, Evalue=5e-66,
Organism=Escherichia coli, GI87081728, Length=322, Percent_Identity=45.9627329192547, Blast_Score=265, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6321398, Length=316, Percent_Identity=37.9746835443038, Blast_Score=202, Evalue=8e-53,
Organism=Drosophila melanogaster, GI21358291, Length=291, Percent_Identity=38.8316151202749, Blast_Score=234, Evalue=8e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38416; Mature: 38285

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKFGRPIEIRDPSTRPAAPPLDLTIRPRRNRKTEWARRLVRENVLTTDDLIWPMFVVD
CCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEC
GTNQRVQVASMPGVERLSVDQIVRDAERAAKLDIPCIALFPFTEPSLRDEHGTEAANPEN
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHH
LICKTVRAIKKEFPQIGVLCDVALDPYTSHGHDGLIEDGRILNDETVAVLVQQSLVQAQA
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHC
GCDVIAPSDMMDGRIGAIREALDAAGYLDVQIMAYAAKYASAFYGPFRDAIGSAKALTGD
CCCEECCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
KRTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFGVPTFAYQVSGE
CCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCC
YAMIAAAANNGWLDGDRAMMESLLAFKRAGANGVLTYFAPAAAEKLRQQG
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCC
>Mature Secondary Structure 
AIKFGRPIEIRDPSTRPAAPPLDLTIRPRRNRKTEWARRLVRENVLTTDDLIWPMFVVD
CCCCCCCEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEC
GTNQRVQVASMPGVERLSVDQIVRDAERAAKLDIPCIALFPFTEPSLRDEHGTEAANPEN
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHH
LICKTVRAIKKEFPQIGVLCDVALDPYTSHGHDGLIEDGRILNDETVAVLVQQSLVQAQA
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHC
GCDVIAPSDMMDGRIGAIREALDAAGYLDVQIMAYAAKYASAFYGPFRDAIGSAKALTGD
CCCEECCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
KRTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFGVPTFAYQVSGE
CCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCC
YAMIAAAANNGWLDGDRAMMESLLAFKRAGANGVLTYFAPAAAEKLRQQG
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]