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The map label for this gene is hipO [H]

Identifier: 192291297

GI number: 192291297

Start: 3186819

End: 3187985

Strand: Reverse

Name: hipO [H]

Synonym: Rpal_2919

Alternate gene names: 192291297

Gene position: 3187985-3186819 (Counterclockwise)

Preceding gene: 192291298

Following gene: 192291296

Centisome position: 55.5

GC content: 63.24

Gene sequence:

>1167_bases
ATGACCATCGATCCCCGCATCACCACCATCGCGGACGAAGCCATCGTTTGGCGCCGAGACATCCACGCTCATCCGGAAAT
TATGTACGACCTCGACCGCACCTCGGCCTTGGTCGCAGATCTGCTGCGTCAGTTCGGGTGCGACGAGGTGGTAACGGGGC
TGGGACGGACCGGCGTTGTTGGCCTGGTACACGGTCGGGGTCGTAGCGCCAATCGCGCCATTGGCCTGCGCGCCGACATG
GATGCGCTGCCCATCGAGGAGGACACCGGCGTTGCCTACGCATCGCAGACGCCCGGCCGAATGCACGCTTGCGGCCATGA
TGGTCACACCGCAATGTTGCTCGGCGCAGCCAAGCATCTGGCCGCCACGCGGGACTTCAACGGCTCGGTGGCATTCATCT
TCCAGCCGGCCGAGGAAGGCGGCGCCGGCGCCAAGGCGATGATCGAGGACGGTCTCTTCAGCCGTTTCCCGATCGATGAG
GTCTATGGCATGCACAACAAACCCGGCCTTCCGATCGGGCAGTTCGCCACCCGCGCAGGCGCGGTCATGGCATCGACCGA
CCGCGTCGAAATCGAGATCGAAGGCATCGGAGGTCACGCCGCGCGGCCGCAGCATTCGATCGATCCGGTTGTCGTCGGGG
CACAGATTGTCACCGCGCTGCAAACGATCGTATCTCGAACGCTCGATCCGCTTTCTTCTGCGGTTGTTTCGATCACCAAC
TTCCATGCCGGCTCGGCGTTCAACGTCATCCCGCAGAAGGCGACTCTGGTCGGCACCGCGCGTGCGCTTGATGGCAAGGT
TCGTGACCGGCTGGAGGCCCGCATTGCGGAGCTCTCAACGCTGATTGCCCAGGCTCACGGGGCGACTGCCCGGGTCACTT
ACGAGCGCGGCTATCCGGTGACCCGCAACCACGCTGAACAGACCGCTTTTGCCGCGGATACGGCCGTTGCAATCACCGGC
AACGACCAGGTCGATCGCGATCTTGCTCCAATGATGGGCGCAGAGGACTTTTCCTACATGCTCGAACAGCGCCCGGGTGC
ACTGATCTTCCTCGGCAATGGCGACAGCGCCGGCCTGCATCATCCGCGCTACGATTTTGCCGATGCTGCGATTCCTTATG
GCATCGCCTACTGGTCCAAGCTTGTGGAGCGCGCGCTGCCTTTGTGA

Upstream 100 bases:

>100_bases
TGAAAGGCGCGCCACCGACGACGCTCGATATCAAGCTGTCGCACGGCGCGCCGCTCGAATTCATCGGCTGACTTGTCCGA
TCAAAGGAGATCATCACAGC

Downstream 100 bases:

>100_bases
GCAGTGCGTTTCTACGCTCGATGAAAAAATAGACCTGCGGCGAGAGCAATCGGTGGAACGGAATCACCCAGAGACCGGAA
AACTCAGCATGGTTTGAACG

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MTIDPRITTIADEAIVWRRDIHAHPEIMYDLDRTSALVADLLRQFGCDEVVTGLGRTGVVGLVHGRGRSANRAIGLRADM
DALPIEEDTGVAYASQTPGRMHACGHDGHTAMLLGAAKHLAATRDFNGSVAFIFQPAEEGGAGAKAMIEDGLFSRFPIDE
VYGMHNKPGLPIGQFATRAGAVMASTDRVEIEIEGIGGHAARPQHSIDPVVVGAQIVTALQTIVSRTLDPLSSAVVSITN
FHAGSAFNVIPQKATLVGTARALDGKVRDRLEARIAELSTLIAQAHGATARVTYERGYPVTRNHAEQTAFAADTAVAITG
NDQVDRDLAPMMGAEDFSYMLEQRPGALIFLGNGDSAGLHHPRYDFADAAIPYGIAYWSKLVERALPL

Sequences:

>Translated_388_residues
MTIDPRITTIADEAIVWRRDIHAHPEIMYDLDRTSALVADLLRQFGCDEVVTGLGRTGVVGLVHGRGRSANRAIGLRADM
DALPIEEDTGVAYASQTPGRMHACGHDGHTAMLLGAAKHLAATRDFNGSVAFIFQPAEEGGAGAKAMIEDGLFSRFPIDE
VYGMHNKPGLPIGQFATRAGAVMASTDRVEIEIEGIGGHAARPQHSIDPVVVGAQIVTALQTIVSRTLDPLSSAVVSITN
FHAGSAFNVIPQKATLVGTARALDGKVRDRLEARIAELSTLIAQAHGATARVTYERGYPVTRNHAEQTAFAADTAVAITG
NDQVDRDLAPMMGAEDFSYMLEQRPGALIFLGNGDSAGLHHPRYDFADAAIPYGIAYWSKLVERALPL
>Mature_387_residues
TIDPRITTIADEAIVWRRDIHAHPEIMYDLDRTSALVADLLRQFGCDEVVTGLGRTGVVGLVHGRGRSANRAIGLRADMD
ALPIEEDTGVAYASQTPGRMHACGHDGHTAMLLGAAKHLAATRDFNGSVAFIFQPAEEGGAGAKAMIEDGLFSRFPIDEV
YGMHNKPGLPIGQFATRAGAVMASTDRVEIEIEGIGGHAARPQHSIDPVVVGAQIVTALQTIVSRTLDPLSSAVVSITNF
HAGSAFNVIPQKATLVGTARALDGKVRDRLEARIAELSTLIAQAHGATARVTYERGYPVTRNHAEQTAFAADTAVAITGN
DQVDRDLAPMMGAEDFSYMLEQRPGALIFLGNGDSAGLHHPRYDFADAAIPYGIAYWSKLVERALPL

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=431, Percent_Identity=27.1461716937355, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41421; Mature: 41290

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDPRITTIADEAIVWRRDIHAHPEIMYDLDRTSALVADLLRQFGCDEVVTGLGRTGVV
CCCCCCEEEEHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHCCCCCCEE
GLVHGRGRSANRAIGLRADMDALPIEEDTGVAYASQTPGRMHACGHDGHTAMLLGAAKHL
EEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCEEECCCCCCEEEEEHHHHHH
AATRDFNGSVAFIFQPAEEGGAGAKAMIEDGLFSRFPIDEVYGMHNKPGLPIGQFATRAG
HHCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHHHHHCC
AVMASTDRVEIEIEGIGGHAARPQHSIDPVVVGAQIVTALQTIVSRTLDPLSSAVVSITN
CEEEECCEEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FHAGSAFNVIPQKATLVGTARALDGKVRDRLEARIAELSTLIAQAHGATARVTYERGYPV
CCCCCEEEECCCCEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
TRNHAEQTAFAADTAVAITGNDQVDRDLAPMMGAEDFSYMLEQRPGALIFLGNGDSAGLH
CCCCHHHHHHHCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCC
HPRYDFADAAIPYGIAYWSKLVERALPL
CCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIDPRITTIADEAIVWRRDIHAHPEIMYDLDRTSALVADLLRQFGCDEVVTGLGRTGVV
CCCCCEEEEHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHCCCCCCEE
GLVHGRGRSANRAIGLRADMDALPIEEDTGVAYASQTPGRMHACGHDGHTAMLLGAAKHL
EEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCEEECCCCCCEEEEEHHHHHH
AATRDFNGSVAFIFQPAEEGGAGAKAMIEDGLFSRFPIDEVYGMHNKPGLPIGQFATRAG
HHCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHHHHHCC
AVMASTDRVEIEIEGIGGHAARPQHSIDPVVVGAQIVTALQTIVSRTLDPLSSAVVSITN
CEEEECCEEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FHAGSAFNVIPQKATLVGTARALDGKVRDRLEARIAELSTLIAQAHGATARVTYERGYPV
CCCCCEEEECCCCEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
TRNHAEQTAFAADTAVAITGNDQVDRDLAPMMGAEDFSYMLEQRPGALIFLGNGDSAGLH
CCCCHHHHHHHCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCC
HPRYDFADAAIPYGIAYWSKLVERALPL
CCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]