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The map label for this gene is metB [H]

Identifier: 192291274

GI number: 192291274

Start: 3159052

End: 3160260

Strand: Reverse

Name: metB [H]

Synonym: Rpal_2896

Alternate gene names: 192291274

Gene position: 3160260-3159052 (Counterclockwise)

Preceding gene: 192291275

Following gene: 192291273

Centisome position: 55.02

GC content: 65.67

Gene sequence:

>1209_bases
ATGACCAAAGCAGCGAAGCAATGGGGCCCGGCTACCTGGGCCCTGTATATGGGTGAGCAACACCGCCAGGTCGGAGATCC
GGTGACGCCCGGCCCTGCTCTCAGTGCGAGCTTTCTCACCGCGCCCAACGCAGTCGGCTTCTCTGCCAACGACCTCGGTG
CCGCCGCGCCGCATTTCTACACAAGATGGAGCAATCCCACCGTCGAGCTGCTGGAGGCGCGGCTGGCGGCGCTCGATGCC
GGCGAAGCCGCGGTGACATTCGCGTCCGGCATGGCCGCCGTGGCCGCGTTGTTTTCCGACCGCCTCCATGCCGGCGACCA
TCTTGTGCTGTCCGACGTCTGCTACGCCGGCGTCGCCGAACTCGCACATGATGAGCTCACTCGCAATGGTATCGCGGTTA
CGACCGTGGATTCGTCGAGGCCCGAGGCGATTGCGGCTGCGCTTCGTCCCAACACCAGGCTGGTTCATATCGAAACGCCG
GCCAATCCGATCCTGCGCCTCGCCGACATCGCCGCTATCGCTCCGCTCGTGCACGCGGCCGGCGCCGAATTGTCGGTCGA
CGCAACGATCGCGACTCCGCTCGGTCTCCAGCCACTCGAACTCGGCGCCGACTACGTCGTCCATTCGCTGACCAAATACA
TCTGCGGTCACGGCGACACGCTCGGCGGCGCGGTGATCGGACGAGCCGACGCGATCGCGCGACTGCGCCGCGGCAGCCTG
ATCCACCTCGGCGGCTGTTTGGCGCCGTTCTCGGCGTGGTTGATCCTACGGGGTCTCGAAACACTCGCGCCGCGGATGCG
GCTGCACGAGGCCAACGCAAGAGCCGTCGAAGCGCTTCTCGCCAACCATCCGAAGGTGAAGGCAGTCTACTGGCCCGGGT
CGCCTCGCCATCCGCAGCACGAGCTGGCGCAACGAACCATGCGTTGCTTCTCAGGCCTGCTCTCGTTCACAGTGAATGGC
GACGGACCGGCATTGGCCGAGCAACTCGCCGAGCGGATGCGCGTCGTCTCCTACGCGGTCTCGCTCGGCAAGACCAAGAG
CCTGCTGGTCTACATCCCGACCGAGGACATTTTGCGCTCGTCGTTCCGACTGACCGACGACGCGGCGGCGAGTTATCGCG
CCTGGGCCGGCGACGGTGTCTTTCGCCTGTCAGTCGGCATCGAGGATTCCGCCGATATCATCGCGGATCTCGAGACCGCA
CTCGGATGA

Upstream 100 bases:

>100_bases
TCGTCCGATGGAGCCGTGACGAACCGACAGGACAGGCATGATCGCAGCGAGCTCCAATCCCCGCTATGGTCGCTGAAGAC
ATGCGACACGGGATGCAACG

Downstream 100 bases:

>100_bases
AGCGGCTCCCGCTGCGGGCGACCGGGCCGGCTGATCTGGAGACTCTCGACCTCGAGGTCAGCGTCGCTCGAGCAACGGCG
ATAGGCCGGTAGCCCCTTCG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTKAAKQWGPATWALYMGEQHRQVGDPVTPGPALSASFLTAPNAVGFSANDLGAAAPHFYTRWSNPTVELLEARLAALDA
GEAAVTFASGMAAVAALFSDRLHAGDHLVLSDVCYAGVAELAHDELTRNGIAVTTVDSSRPEAIAAALRPNTRLVHIETP
ANPILRLADIAAIAPLVHAAGAELSVDATIATPLGLQPLELGADYVVHSLTKYICGHGDTLGGAVIGRADAIARLRRGSL
IHLGGCLAPFSAWLILRGLETLAPRMRLHEANARAVEALLANHPKVKAVYWPGSPRHPQHELAQRTMRCFSGLLSFTVNG
DGPALAEQLAERMRVVSYAVSLGKTKSLLVYIPTEDILRSSFRLTDDAAASYRAWAGDGVFRLSVGIEDSADIIADLETA
LG

Sequences:

>Translated_402_residues
MTKAAKQWGPATWALYMGEQHRQVGDPVTPGPALSASFLTAPNAVGFSANDLGAAAPHFYTRWSNPTVELLEARLAALDA
GEAAVTFASGMAAVAALFSDRLHAGDHLVLSDVCYAGVAELAHDELTRNGIAVTTVDSSRPEAIAAALRPNTRLVHIETP
ANPILRLADIAAIAPLVHAAGAELSVDATIATPLGLQPLELGADYVVHSLTKYICGHGDTLGGAVIGRADAIARLRRGSL
IHLGGCLAPFSAWLILRGLETLAPRMRLHEANARAVEALLANHPKVKAVYWPGSPRHPQHELAQRTMRCFSGLLSFTVNG
DGPALAEQLAERMRVVSYAVSLGKTKSLLVYIPTEDILRSSFRLTDDAAASYRAWAGDGVFRLSVGIEDSADIIADLETA
LG
>Mature_401_residues
TKAAKQWGPATWALYMGEQHRQVGDPVTPGPALSASFLTAPNAVGFSANDLGAAAPHFYTRWSNPTVELLEARLAALDAG
EAAVTFASGMAAVAALFSDRLHAGDHLVLSDVCYAGVAELAHDELTRNGIAVTTVDSSRPEAIAAALRPNTRLVHIETPA
NPILRLADIAAIAPLVHAAGAELSVDATIATPLGLQPLELGADYVVHSLTKYICGHGDTLGGAVIGRADAIARLRRGSLI
HLGGCLAPFSAWLILRGLETLAPRMRLHEANARAVEALLANHPKVKAVYWPGSPRHPQHELAQRTMRCFSGLLSFTVNGD
GPALAEQLAERMRVVSYAVSLGKTKSLLVYIPTEDILRSSFRLTDDAAASYRAWAGDGVFRLSVGIEDSADIIADLETAL
G

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=343, Percent_Identity=34.9854227405248, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI299473759, Length=343, Percent_Identity=32.0699708454811, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI25453487, Length=342, Percent_Identity=29.5321637426901, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1790375, Length=394, Percent_Identity=35.5329949238579, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789383, Length=347, Percent_Identity=28.5302593659942, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=357, Percent_Identity=34.1736694677871, Blast_Score=221, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17538055, Length=385, Percent_Identity=34.8051948051948, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=347, Percent_Identity=34.5821325648415, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319307, Length=405, Percent_Identity=33.0864197530864, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6323333, Length=397, Percent_Identity=28.9672544080605, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=298, Percent_Identity=29.8657718120805, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=356, Percent_Identity=28.0898876404494, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321254, Length=412, Percent_Identity=25.7281553398058, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922554, Length=352, Percent_Identity=36.9318181818182, Blast_Score=218, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 42488; Mature: 42357

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAAKQWGPATWALYMGEQHRQVGDPVTPGPALSASFLTAPNAVGFSANDLGAAAPHFY
CCCCCHHCCCEEEEEEECCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCE
TRWSNPTVELLEARLAALDAGEAAVTFASGMAAVAALFSDRLHAGDHLVLSDVCYAGVAE
ECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
LAHDELTRNGIAVTTVDSSRPEAIAAALRPNTRLVHIETPANPILRLADIAAIAPLVHAA
HHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHC
GAELSVDATIATPLGLQPLELGADYVVHSLTKYICGHGDTLGGAVIGRADAIARLRRGSL
CCCEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCE
IHLGGCLAPFSAWLILRGLETLAPRMRLHEANARAVEALLANHPKVKAVYWPGSPRHPQH
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEECCCCCCCCHH
ELAQRTMRCFSGLLSFTVNGDGPALAEQLAERMRVVSYAVSLGKTKSLLVYIPTEDILRS
HHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
SFRLTDDAAASYRAWAGDGVFRLSVGIEDSADIIADLETALG
HCCCCCHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TKAAKQWGPATWALYMGEQHRQVGDPVTPGPALSASFLTAPNAVGFSANDLGAAAPHFY
CCCCHHCCCEEEEEEECCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCE
TRWSNPTVELLEARLAALDAGEAAVTFASGMAAVAALFSDRLHAGDHLVLSDVCYAGVAE
ECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
LAHDELTRNGIAVTTVDSSRPEAIAAALRPNTRLVHIETPANPILRLADIAAIAPLVHAA
HHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHC
GAELSVDATIATPLGLQPLELGADYVVHSLTKYICGHGDTLGGAVIGRADAIARLRRGSL
CCCEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCE
IHLGGCLAPFSAWLILRGLETLAPRMRLHEANARAVEALLANHPKVKAVYWPGSPRHPQH
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEECCCCCCCCHH
ELAQRTMRCFSGLLSFTVNGDGPALAEQLAERMRVVSYAVSLGKTKSLLVYIPTEDILRS
HHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
SFRLTDDAAASYRAWAGDGVFRLSVGIEDSADIIADLETALG
HCCCCCHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]