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The map label for this gene is pleD [H]

Identifier: 192291133

GI number: 192291133

Start: 2995318

End: 2996385

Strand: Reverse

Name: pleD [H]

Synonym: Rpal_2754

Alternate gene names: 192291133

Gene position: 2996385-2995318 (Counterclockwise)

Preceding gene: 192291134

Following gene: 192291119

Centisome position: 52.17

GC content: 65.07

Gene sequence:

>1068_bases
GTGGCCGCGCTGCAGGACGACCATGACCGCACCATGGCCTATGCCGAGGTGGCCCTCTCCCAGATCAAGACCCTCCGCCA
AGGCGCCGTGCCGCGCAATTACGAGATCTGGTACGTCTACGCCACCGGCCATAATCCCGAGCTGAACAAGGCGATCAACG
ACACGCTGGCGGAGCGCGGCAACCTCACCGACGCCGACCTCGATCAGTTGCACGATACCTACCTCACGCAAGGCCGCACC
ACCGACCGGATCGACCGGATCGGCGCCCGCGTCGCCAACGAGATCGACGACATCATGGCGCTGCTCGCCGAGACGCTCGG
CAACACCGCCAGCTTCGGCGACGATCTGCGCGGCGAAAGCCAGAAGCTGTCGCTGGCGACCGACCACGATCAGATCAAAG
CGATCGTCGAGCGTCTGGTCGTGACCACCCGCGAGATGCAGCAGGCCAACAGCGCGCTCGAAGAGCGGCTGTCGACCTCC
AAGCAGGAGATCGCCAATCTCCAGGTCAGCCTCGATGCGATCCGCGCCGAGAGCCTCACCGATCCCCTCACCGGCCTCGG
CAACCGCAAGCATTTCGACCGCACCGTCCAGGAGGCGATCCGCAACGCCGGCGCCAGCCAGCAGCCACTGTCGCTGCTGA
TGATGGACATCGATCACTTCAAGTCATTCAACGACACCTACGGCCATCTCACCGGCGACCAGGTGCTGCGGCTCGTCGGC
ATGACGCTGAAGCAGAGCATCAAGGGCCAGGACTTCACCGCCCGCTACGGCGGCGAGGAATTCGCCGTGGTGCTGCCGAA
CACGCCGATGCGCCAGGCGATCATCGTCGCCGACAACATCCGCCGGCTGGTGATGTCGAAGGAGCTGAAGAAGAAGTCGA
CCGGCGAGATCCTCGGCCGGGTGACGCTATCGGCCGGCGTGTCGACGCTGAAGCCGAATGACGATGCCGAAGCGCTGATC
GAGCGCGCCGACGCCTGCCTCTATGCCGCCAAACGCAACGGCCGCAACCGGGTGATCTGCGAGACAGATCCGGAATTTGC
CGGCATCACGCAGGCCGCCGTTGCCTGA

Upstream 100 bases:

>100_bases
GATGGGCGATTGGGCGGTGTTTACGAGGCCTTCACCACCCGCGCCGTAACCTTACACCGTTTCAAACAAGCGTTGAAAAC
GCCTGATCGGAGGGTGGCTC

Downstream 100 bases:

>100_bases
CGTCGGCGTCAGCGCGCAGCCCGGCTGCGCTTCGCTGCTGCTGCCTTCGTGGTCTTCGCCTTCTTCGCCGAAGCTGCCGT
CTTCACGGCTGTCTTGACCT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MAALQDDHDRTMAYAEVALSQIKTLRQGAVPRNYEIWYVYATGHNPELNKAINDTLAERGNLTDADLDQLHDTYLTQGRT
TDRIDRIGARVANEIDDIMALLAETLGNTASFGDDLRGESQKLSLATDHDQIKAIVERLVVTTREMQQANSALEERLSTS
KQEIANLQVSLDAIRAESLTDPLTGLGNRKHFDRTVQEAIRNAGASQQPLSLLMMDIDHFKSFNDTYGHLTGDQVLRLVG
MTLKQSIKGQDFTARYGGEEFAVVLPNTPMRQAIIVADNIRRLVMSKELKKKSTGEILGRVTLSAGVSTLKPNDDAEALI
ERADACLYAAKRNGRNRVICETDPEFAGITQAAVA

Sequences:

>Translated_355_residues
MAALQDDHDRTMAYAEVALSQIKTLRQGAVPRNYEIWYVYATGHNPELNKAINDTLAERGNLTDADLDQLHDTYLTQGRT
TDRIDRIGARVANEIDDIMALLAETLGNTASFGDDLRGESQKLSLATDHDQIKAIVERLVVTTREMQQANSALEERLSTS
KQEIANLQVSLDAIRAESLTDPLTGLGNRKHFDRTVQEAIRNAGASQQPLSLLMMDIDHFKSFNDTYGHLTGDQVLRLVG
MTLKQSIKGQDFTARYGGEEFAVVLPNTPMRQAIIVADNIRRLVMSKELKKKSTGEILGRVTLSAGVSTLKPNDDAEALI
ERADACLYAAKRNGRNRVICETDPEFAGITQAAVA
>Mature_354_residues
AALQDDHDRTMAYAEVALSQIKTLRQGAVPRNYEIWYVYATGHNPELNKAINDTLAERGNLTDADLDQLHDTYLTQGRTT
DRIDRIGARVANEIDDIMALLAETLGNTASFGDDLRGESQKLSLATDHDQIKAIVERLVVTTREMQQANSALEERLSTSK
QEIANLQVSLDAIRAESLTDPLTGLGNRKHFDRTVQEAIRNAGASQQPLSLLMMDIDHFKSFNDTYGHLTGDQVLRLVGM
TLKQSIKGQDFTARYGGEEFAVVLPNTPMRQAIIVADNIRRLVMSKELKKKSTGEILGRVTLSAGVSTLKPNDDAEALIE
RADACLYAAKRNGRNRVICETDPEFAGITQAAVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=39.6341463414634, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=39.622641509434, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082007, Length=182, Percent_Identity=34.6153846153846, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787816, Length=204, Percent_Identity=34.8039215686275, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=37.8698224852071, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=33.9393939393939, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787802, Length=199, Percent_Identity=33.1658291457286, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=31.4465408805031, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=29.1925465838509, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788956, Length=185, Percent_Identity=31.8918918918919, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787056, Length=171, Percent_Identity=32.1637426900585, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39170; Mature: 39038

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALQDDHDRTMAYAEVALSQIKTLRQGAVPRNYEIWYVYATGHNPELNKAINDTLAERG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCC
NLTDADLDQLHDTYLTQGRTTDRIDRIGARVANEIDDIMALLAETLGNTASFGDDLRGES
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
QKLSLATDHDQIKAIVERLVVTTREMQQANSALEERLSTSKQEIANLQVSLDAIRAESLT
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPLTGLGNRKHFDRTVQEAIRNAGASQQPLSLLMMDIDHFKSFNDTYGHLTGDQVLRLVG
CHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
MTLKQSIKGQDFTARYGGEEFAVVLPNTPMRQAIIVADNIRRLVMSKELKKKSTGEILGR
HHHHHHCCCCCCEEECCCCEEEEECCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VTLSAGVSTLKPNDDAEALIERADACLYAAKRNGRNRVICETDPEFAGITQAAVA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHCCHHHHCC
>Mature Secondary Structure 
AALQDDHDRTMAYAEVALSQIKTLRQGAVPRNYEIWYVYATGHNPELNKAINDTLAERG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCC
NLTDADLDQLHDTYLTQGRTTDRIDRIGARVANEIDDIMALLAETLGNTASFGDDLRGES
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
QKLSLATDHDQIKAIVERLVVTTREMQQANSALEERLSTSKQEIANLQVSLDAIRAESLT
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPLTGLGNRKHFDRTVQEAIRNAGASQQPLSLLMMDIDHFKSFNDTYGHLTGDQVLRLVG
CHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
MTLKQSIKGQDFTARYGGEEFAVVLPNTPMRQAIIVADNIRRLVMSKELKKKSTGEILGR
HHHHHHCCCCCCEEECCCCEEEEECCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VTLSAGVSTLKPNDDAEALIERADACLYAAKRNGRNRVICETDPEFAGITQAAVA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA