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The map label for this gene is tyrS [H]

Identifier: 192291117

GI number: 192291117

Start: 2978285

End: 2979541

Strand: Reverse

Name: tyrS [H]

Synonym: Rpal_2738

Alternate gene names: 192291117

Gene position: 2979541-2978285 (Counterclockwise)

Preceding gene: 192291119

Following gene: 192291116

Centisome position: 51.87

GC content: 63.09

Gene sequence:

>1257_bases
ATGACCGCTTTTAAGTCTGATTTTATGAATGTTCTCCAAAGTCGGGGATTCATTCATCAGATTTCCGACCCCGACTCGCT
GGACGCGCTCGCGGCGAAGGGCGAAGTGGTCGCGTATGTCGGTTACGACTGCACCGCGGCCTCGCTGCATGTCGGGCATC
TGCTCTCGATCATGATGCTGCATTGGCTGCAGGCGACCGGCAACAAGCCGATCGCACTGATGGGCGGCGGCACCACGCGC
GTCGGCGATCCGTCGGGCCGTGACGAGACCCGTAAGATCCTCACCTACGAGCAGATCGACGCCAACAAGGAGTCGATCAA
GGGCACGTTCTCGAAGTTCATCAAGTTCGGCGACGGTCACAGCGACGCGCTGATGGCCGACAACGCCGAGTGGCTGACCA
AGCTGAACTACATCGAGATGCTGCGGGAGATCGGTCGCCACTTCTCGATCAACCGCATGCTGACGATGGACTCGGTGAAG
CTGCGGCTCGACCGCGAGCAGGAGCTGTCGTTCATCGAATTCAACTACATGATCCTGCAGTCCTACGACTTCGTCGAGCT
GGCGCGGCGCTACAAATGCAATCTGCAGATGGGCGGCTCGGATCAGTGGGGCAACATCGTCACCGGCGTCGATCTCGGCC
GCCGGATGGGCACCCACCAGCTTCACGCGCTGACCTGCCCGCTCCTCACCACCGCCTCGGGCGCCAAGATGGGCAAGACC
GCCGCTGGCGCCGTCTGGCTCAACGGCGACATGCTGGCGCCGTACGACTACTGGCAGTATTGGCGCAACACCGAGGACGC
CGACGTCGGCCGCTTCCTCAAGCTGTTCACGCTGCTGCCGATGGACGAGATCGAGCGGCTATCGAAGCTGCAGGGCGCCG
AGATCAACGACGCCAAGAAGATCCTGGCCACCGAGGCGACCGCGCTGATGCACGGCCGCGAAGCGGCAGACAAGGCGGAA
GCCACAGCCCGCACCACCTTCGAACAGGGTGGCACCGCGCAGGATCTGCCGAGCGTCGAGATCAGCCGCGGCGAGCTCGA
TGCCGGGATCGGCGTGCTGGTGGCGTTCGCGGAAAAGACCGGGCTGGTCGCCTCCAACGGCGAAGCGCGCCGCCAGATCA
AGGCCGGTGGCCTCAAGGTCAATGATGCGCCGGTGATCGACGAGAAGATGACGCTGACCGCGACCGACCTGTCGTCGGAC
GGCGTGATCAAGCTGTCGATGGGCAAGAAGAAGCACGTGCTGCTTCGGCTTGCATAG

Upstream 100 bases:

>100_bases
TTATAATGCCACAGGGTAACGCGCCCGAAAGACATTCCTGGGCGTCTTCGCGCGCGATCTGCGCGATGCGTGAAATAACC
GTGATCAGAGACCGATACAG

Downstream 100 bases:

>100_bases
CCCCATTCGCGGGCGGGCCCTGCTCCTTTCCGGCAAAACCGCGTTCGATGTGAAAAACGCGGACTCGTTCGGGAAGGATC
GTCCTTGGATCACCGTGCCT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTAFKSDFMNVLQSRGFIHQISDPDSLDALAAKGEVVAYVGYDCTAASLHVGHLLSIMMLHWLQATGNKPIALMGGGTTR
VGDPSGRDETRKILTYEQIDANKESIKGTFSKFIKFGDGHSDALMADNAEWLTKLNYIEMLREIGRHFSINRMLTMDSVK
LRLDREQELSFIEFNYMILQSYDFVELARRYKCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTASGAKMGKT
AAGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPMDEIERLSKLQGAEINDAKKILATEATALMHGREAADKAE
ATARTTFEQGGTAQDLPSVEISRGELDAGIGVLVAFAEKTGLVASNGEARRQIKAGGLKVNDAPVIDEKMTLTATDLSSD
GVIKLSMGKKKHVLLRLA

Sequences:

>Translated_418_residues
MTAFKSDFMNVLQSRGFIHQISDPDSLDALAAKGEVVAYVGYDCTAASLHVGHLLSIMMLHWLQATGNKPIALMGGGTTR
VGDPSGRDETRKILTYEQIDANKESIKGTFSKFIKFGDGHSDALMADNAEWLTKLNYIEMLREIGRHFSINRMLTMDSVK
LRLDREQELSFIEFNYMILQSYDFVELARRYKCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTASGAKMGKT
AAGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPMDEIERLSKLQGAEINDAKKILATEATALMHGREAADKAE
ATARTTFEQGGTAQDLPSVEISRGELDAGIGVLVAFAEKTGLVASNGEARRQIKAGGLKVNDAPVIDEKMTLTATDLSSD
GVIKLSMGKKKHVLLRLA
>Mature_417_residues
TAFKSDFMNVLQSRGFIHQISDPDSLDALAAKGEVVAYVGYDCTAASLHVGHLLSIMMLHWLQATGNKPIALMGGGTTRV
GDPSGRDETRKILTYEQIDANKESIKGTFSKFIKFGDGHSDALMADNAEWLTKLNYIEMLREIGRHFSINRMLTMDSVKL
RLDREQELSFIEFNYMILQSYDFVELARRYKCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTASGAKMGKTA
AGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPMDEIERLSKLQGAEINDAKKILATEATALMHGREAADKAEA
TARTTFEQGGTAQDLPSVEISRGELDAGIGVLVAFAEKTGLVASNGEARRQIKAGGLKVNDAPVIDEKMTLTATDLSSDG
VIKLSMGKKKHVLLRLA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=417, Percent_Identity=34.5323741007194, Blast_Score=232, Evalue=5e-61,
Organism=Escherichia coli, GI1787925, Length=426, Percent_Identity=38.2629107981221, Blast_Score=298, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI25145802, Length=314, Percent_Identity=32.8025477707006, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI25145805, Length=250, Percent_Identity=30, Blast_Score=121, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6325160, Length=439, Percent_Identity=30.751708428246, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI20130383, Length=440, Percent_Identity=34.5454545454545, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46021; Mature: 45890

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFKSDFMNVLQSRGFIHQISDPDSLDALAAKGEVVAYVGYDCTAASLHVGHLLSIMML
CCCHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
HWLQATGNKPIALMGGGTTRVGDPSGRDETRKILTYEQIDANKESIKGTFSKFIKFGDGH
HHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHEEEHHHHCCCHHHHHHHHHHHHHCCCCC
SDALMADNAEWLTKLNYIEMLREIGRHFSINRMLTMDSVKLRLDREQELSFIEFNYMILQ
CCCEEECCHHHHHHHHHHHHHHHHHHCEECCEEEEECCEEEEECCCCCCCEEEEEEEEEE
SYDFVELARRYKCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTASGAKMGKT
CCCHHHHHHHHCCEEEECCCCCCCCEEEHHHHHHHCCCCHHHEEECCHHCCCCCCCCCCC
AAGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPMDEIERLSKLQGAEINDAKK
CCCEEEECCCEECCHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHH
ILATEATALMHGREAADKAEATARTTFEQGGTAQDLPSVEISRGELDAGIGVLVAFAEKT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEEHHHC
GLVASNGEARRQIKAGGLKVNDAPVIDEKMTLTATDLSSDGVIKLSMGKKKHVLLRLA
CCEECCCHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
TAFKSDFMNVLQSRGFIHQISDPDSLDALAAKGEVVAYVGYDCTAASLHVGHLLSIMML
CCHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
HWLQATGNKPIALMGGGTTRVGDPSGRDETRKILTYEQIDANKESIKGTFSKFIKFGDGH
HHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHEEEHHHHCCCHHHHHHHHHHHHHCCCCC
SDALMADNAEWLTKLNYIEMLREIGRHFSINRMLTMDSVKLRLDREQELSFIEFNYMILQ
CCCEEECCHHHHHHHHHHHHHHHHHHCEECCEEEEECCEEEEECCCCCCCEEEEEEEEEE
SYDFVELARRYKCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTASGAKMGKT
CCCHHHHHHHHCCEEEECCCCCCCCEEEHHHHHHHCCCCHHHEEECCHHCCCCCCCCCCC
AAGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPMDEIERLSKLQGAEINDAKK
CCCEEEECCCEECCHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHH
ILATEATALMHGREAADKAEATARTTFEQGGTAQDLPSVEISRGELDAGIGVLVAFAEKT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEEHHHC
GLVASNGEARRQIKAGGLKVNDAPVIDEKMTLTATDLSSDGVIKLSMGKKKHVLLRLA
CCEECCCHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA