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The map label for this gene is nifE [H]

Identifier: 192290974

GI number: 192290974

Start: 2800585

End: 2801976

Strand: Reverse

Name: nifE [H]

Synonym: Rpal_2591

Alternate gene names: 192290974

Gene position: 2801976-2800585 (Counterclockwise)

Preceding gene: 192290975

Following gene: 192290973

Centisome position: 48.78

GC content: 67.31

Gene sequence:

>1392_bases
ATGAGCATCACCCCGCTTCAAGTCCGTCGCTCCGGGCCGCCCCCTGTCGTCGCAGACGTCCCCGAAGAAGGCCCGATCGA
ACAGGTCCGCTACGCCTGCGCACTCGGCGCAATGCAGACCGTGGCGGCGATCCCGCGCGCGATCCCGATCACCCATTGCG
GTCCAGGCTGCGCCGACAAGCAATGCGGCGCTCTGGCGTTCAACAATGCCTATCAGGGTGGCGGCTGGGGCGGACTCGGC
GTCGCTCCGAGCACCAACGCTACCCAGCGCGAAGTGATCTTCGGCGGCGAAAACCGGCTGCGCGAACTGGTCACCCACAC
GCTGGACCTGATGGAAGCCGATCTGTTCGTCGTGCTCACCGGCTGCATCCCGGATCTGATCGGCGATGACGTGCCGGCCG
TGGTCAAGGAATTCCAGGCCAAGGGCGCCCCGGTGGTCTATGCCGGCACCGGCGGCTTCCGTGGCAACAACTACACCGGC
CACGAGGTGGTCACCCGCGCGATCATCGATCAGTTCGCCAGCGCGGACGCGGGGCCGCGCGAACACGGCCTCGTCAATCT
GTGGGCACCGCTGCCGTATCAGGACCCGTTCTGGCGCGGCGATCTCGCCGAATTGAAGCGGCTACTGCAAGGCATCGGGC
TCCGCGTCAACGTGCTGTTCGGACCGGGCTCGGCCGGCGTGTCGGAATGGCGCGCGATTCCGCGGGCGCAATTCAATCTG
GTGCTGTCGCCCTGGCTCGGGCTCGCCACCGCACAGCATCTCGAAGAGAAATTCGGACAGCCGTATCTCCATCTCCCGGT
GCCCCCGATCGGCGGCCGGCAGACCAGCGAGTTCCTGCGCCAGGTCGCCGCCTTCGCCGGCATTCCGGACGCGCAGGCCG
GCGCCTTCATCGCTGCGGAAGAGCGCGATTACTACGGCTACATCGAGGCGTTCTCGGAGTTCTATTCGCAGTATCGCTGG
GGCCTGCCCGCGCGGTTCGCCGCCATCGGTGATGCCGCCACCACGCTGTCGATCAGCAGCTTCGCGGTCCGCCAGCTCGG
CCTGGTGCCGGCCGTCGTGGTGATCACCGACAACACCCCGCCGGAGCACCGTGACGCGATCCGTGCGGCATTCGCCAAGC
TCGATCACGACGTCGGCATCGAGCCGGTGTTCGCAGAGGACAGCTACGCGGTGCACGCCGCCATCCGCGGTGCCGATTTC
GGCCGCAAGCCGCCGCTGATCTTCGGGACAACCTGGGAGCGTGACCTCGCCAAGGAGCTGCGCGGCTTCATTCTCGAAGT
CGGCTTCCCGGCCTCCTACGAAGTGGTGCTGTCGCGCGGCTATGCCGGCTATCGCGGCGCGCTCACCCTGATCGAGCGCA
TCTACACCACCGCAATCAGCCGCAGCGCCTGA

Upstream 100 bases:

>100_bases
GCCACACCAGCCTGCCCTACAAGCAATGGTGGCTGGATCAAGCCGATCCGTATCTGCTCGCCCGGCAGAACGCCCCGCAA
GCCGCCGGAAGTCGCGTCTG

Downstream 100 bases:

>100_bases
TCGGCGCGCCGGCTGCGAGCGATACGACCAGACGAGATAACGTGACGATCATCTCCAAGCCGCCGTCCGATCCGATGCAG
CCCACCGGATCACGCTTCCG

Product: Nitrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSITPLQVRRSGPPPVVADVPEEGPIEQVRYACALGAMQTVAAIPRAIPITHCGPGCADKQCGALAFNNAYQGGGWGGLG
VAPSTNATQREVIFGGENRLRELVTHTLDLMEADLFVVLTGCIPDLIGDDVPAVVKEFQAKGAPVVYAGTGGFRGNNYTG
HEVVTRAIIDQFASADAGPREHGLVNLWAPLPYQDPFWRGDLAELKRLLQGIGLRVNVLFGPGSAGVSEWRAIPRAQFNL
VLSPWLGLATAQHLEEKFGQPYLHLPVPPIGGRQTSEFLRQVAAFAGIPDAQAGAFIAAEERDYYGYIEAFSEFYSQYRW
GLPARFAAIGDAATTLSISSFAVRQLGLVPAVVVITDNTPPEHRDAIRAAFAKLDHDVGIEPVFAEDSYAVHAAIRGADF
GRKPPLIFGTTWERDLAKELRGFILEVGFPASYEVVLSRGYAGYRGALTLIERIYTTAISRSA

Sequences:

>Translated_463_residues
MSITPLQVRRSGPPPVVADVPEEGPIEQVRYACALGAMQTVAAIPRAIPITHCGPGCADKQCGALAFNNAYQGGGWGGLG
VAPSTNATQREVIFGGENRLRELVTHTLDLMEADLFVVLTGCIPDLIGDDVPAVVKEFQAKGAPVVYAGTGGFRGNNYTG
HEVVTRAIIDQFASADAGPREHGLVNLWAPLPYQDPFWRGDLAELKRLLQGIGLRVNVLFGPGSAGVSEWRAIPRAQFNL
VLSPWLGLATAQHLEEKFGQPYLHLPVPPIGGRQTSEFLRQVAAFAGIPDAQAGAFIAAEERDYYGYIEAFSEFYSQYRW
GLPARFAAIGDAATTLSISSFAVRQLGLVPAVVVITDNTPPEHRDAIRAAFAKLDHDVGIEPVFAEDSYAVHAAIRGADF
GRKPPLIFGTTWERDLAKELRGFILEVGFPASYEVVLSRGYAGYRGALTLIERIYTTAISRSA
>Mature_462_residues
SITPLQVRRSGPPPVVADVPEEGPIEQVRYACALGAMQTVAAIPRAIPITHCGPGCADKQCGALAFNNAYQGGGWGGLGV
APSTNATQREVIFGGENRLRELVTHTLDLMEADLFVVLTGCIPDLIGDDVPAVVKEFQAKGAPVVYAGTGGFRGNNYTGH
EVVTRAIIDQFASADAGPREHGLVNLWAPLPYQDPFWRGDLAELKRLLQGIGLRVNVLFGPGSAGVSEWRAIPRAQFNLV
LSPWLGLATAQHLEEKFGQPYLHLPVPPIGGRQTSEFLRQVAAFAGIPDAQAGAFIAAEERDYYGYIEAFSEFYSQYRWG
LPARFAAIGDAATTLSISSFAVRQLGLVPAVVVITDNTPPEHRDAIRAAFAKLDHDVGIEPVFAEDSYAVHAAIRGADFG
RKPPLIFGTTWERDLAKELRGFILEVGFPASYEVVLSRGYAGYRGALTLIERIYTTAISRSA

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 49886; Mature: 49755

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITPLQVRRSGPPPVVADVPEEGPIEQVRYACALGAMQTVAAIPRAIPITHCGPGCADK
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
QCGALAFNNAYQGGGWGGLGVAPSTNATQREVIFGGENRLRELVTHTLDLMEADLFVVLT
CCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
GCIPDLIGDDVPAVVKEFQAKGAPVVYAGTGGFRGNNYTGHEVVTRAIIDQFASADAGPR
HHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
EHGLVNLWAPLPYQDPFWRGDLAELKRLLQGIGLRVNVLFGPGSAGVSEWRAIPRAQFNL
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCHHHEE
VLSPWLGLATAQHLEEKFGQPYLHLPVPPIGGRQTSEFLRQVAAFAGIPDAQAGAFIAAE
EECHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
ERDYYGYIEAFSEFYSQYRWGLPARFAAIGDAATTLSISSFAVRQLGLVPAVVVITDNTP
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHEEHHHHHHHHHCCCEEEEEEECCCC
PEHRDAIRAAFAKLDHDVGIEPVFAEDSYAVHAAIRGADFGRKPPLIFGTTWERDLAKEL
CHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHH
RGFILEVGFPASYEVVLSRGYAGYRGALTLIERIYTTAISRSA
HHHEEECCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SITPLQVRRSGPPPVVADVPEEGPIEQVRYACALGAMQTVAAIPRAIPITHCGPGCADK
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
QCGALAFNNAYQGGGWGGLGVAPSTNATQREVIFGGENRLRELVTHTLDLMEADLFVVLT
CCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
GCIPDLIGDDVPAVVKEFQAKGAPVVYAGTGGFRGNNYTGHEVVTRAIIDQFASADAGPR
HHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
EHGLVNLWAPLPYQDPFWRGDLAELKRLLQGIGLRVNVLFGPGSAGVSEWRAIPRAQFNL
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCHHHEE
VLSPWLGLATAQHLEEKFGQPYLHLPVPPIGGRQTSEFLRQVAAFAGIPDAQAGAFIAAE
EECHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
ERDYYGYIEAFSEFYSQYRWGLPARFAAIGDAATTLSISSFAVRQLGLVPAVVVITDNTP
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHEEHHHHHHHHHCCCEEEEEEECCCC
PEHRDAIRAAFAKLDHDVGIEPVFAEDSYAVHAAIRGADFGRKPPLIFGTTWERDLAKEL
CHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHH
RGFILEVGFPASYEVVLSRGYAGYRGALTLIERIYTTAISRSA
HHHEEECCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]