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The map label for this gene is nifE [H]

Identifier: 192290975

GI number: 192290975

Start: 2801976

End: 2803511

Strand: Reverse

Name: nifE [H]

Synonym: Rpal_2592

Alternate gene names: 192290975

Gene position: 2803511-2801976 (Counterclockwise)

Preceding gene: 192290976

Following gene: 192290974

Centisome position: 48.81

GC content: 66.34

Gene sequence:

>1536_bases
ATGGCCATTAATCTTCGTAGTCCGTCCGCCGAGACCCGCGAACAGCGTCTTGGTACCATCACCTCATGGGATGGCCCCGC
CTCCCGCCTTGCCAACGAGTCCGCCTTCTCCCGCGGCGATTGCGGCCCCGGATGCGGCGGCAAGGGCCAGCGCATCTGCG
AATTGGAGTCGCCGTTCTCGCAGGGCTCGACCTGCTCCGAGCAGATCGCCGAGAGCCAGGCCAGCAACGTCCGCGACGCA
GTACTGGTGCAGCATTCGCCGATCGGCTGCGCAGCCGGTCATATCAACAGCAACGCGTTCTTCCGCAACGGCCTGATGCG
CCGCGGCCTGGCTCCGCGCAACGTCACCGGCCTGTCGAGCAATCTGATCGAACGCGACATGATCTATGGCGGCGCCGAGA
AGCTGCGCGCCACGATCAAGGCCGCGGTCGAGCGACACCAGCCCAAGGCGGTGTTCGTCGCCACCTCCTGTGCCACCGGC
ATCATCGGCGACGACGTCGAGAGCGTGGTGCGCGACTGCGAGGACGAGCTCGGTGTCCCGGTGGTGGCGATGTATTGCGA
GGGCTTCAAGTCCAAGCATTGGAGCTCAGGCTTCGACGCGATCCAGCACGGCGTGCTGCGCCATGTCGTCAAGCCCAAGC
CGGGCCCGCGGCAGGACAACCTCGTCAATATCATCGTGCTGTGGGGCAGCGACGTGTTCACGCCGATGCTGGCGGAACTG
GGCCTCGAAGCCAACAACATCCTGACCGTCGCCTCGGTCGACGAGATCGCGCGCGCCTCGGAAGCCGCCGCATCGGCGAC
CTTCTGCTACTCGGTCGGTGGCTATCTCGGCGCCGCGCTGGAGGAGCAATACGGCGTGCAGGAGATCAAGGCGCCGCAGC
CTTACGGCTTCGACGGCACCGACGCATGGCTGCGCGCGCTGGCCAAGGCCACCGGCCGGGAAGAACGCGCCGAAGCCTAT
ATCGCCCGCGAGCACGCCCGCGTCCGACCGCAAATCGAGCGGTTGCGCGAGCGCCTCAAGGGCGTGCGCGGCTACGTCGC
GATGGGAGCGGCCTATGCCCACGGCGTCATCGGGGTGCTGCGCGAACTCGGCGTCGAGGTGCCCGGCTCGCTCGTGTTCC
ATCACGACCCGGTCTATGACAGCCACGACGTCCGCCAGGATACGCTCGGCCACATGATCGACGCCTATGGCGATGTCGAG
CACTTCAGCATCTCCAATCGCCAGCCGTATCAGTTCTACAATCTGCTGAAGCGCTCCGATCCGGATTTCATCATCATCCG
CCACGGGGGCCTCGCCGGCCTCGCCTCGCGCCTCGGCGTGCCGGCGATCGCGCTCGGCGATGCGTCGACCGCGATCGGCT
ATCAGGGCATGATCGATCTCGGCGAGGAAATCGTCGATGCGCTGGCGCAGCGCAAATTCCATCAGGACCTCGCCGGCCAC
ACCAGCCTGCCCTACAAGCAATGGTGGCTGGATCAAGCCGATCCGTATCTGCTCGCCCGGCAGAACGCCCCGCAAGCCGC
CGGAAGTCGCGTCTGA

Upstream 100 bases:

>100_bases
CTAGCGCGCTTTCGCGCTTGCGAACGAGGTTTCATCATGGCCATCTTCGCGCGCAACTGATCATCCTCACAGTTTCTTTC
TTTGAAGGCGTTGCGCGCCG

Downstream 100 bases:

>100_bases
TGAGCATCACCCCGCTTCAAGTCCGTCGCTCCGGGCCGCCCCCTGTCGTCGCAGACGTCCCCGAAGAAGGCCCGATCGAA
CAGGTCCGCTACGCCTGCGC

Product: Nitrogenase

Products: oxidized ferredoxin; NH3; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MAINLRSPSAETREQRLGTITSWDGPASRLANESAFSRGDCGPGCGGKGQRICELESPFSQGSTCSEQIAESQASNVRDA
VLVQHSPIGCAAGHINSNAFFRNGLMRRGLAPRNVTGLSSNLIERDMIYGGAEKLRATIKAAVERHQPKAVFVATSCATG
IIGDDVESVVRDCEDELGVPVVAMYCEGFKSKHWSSGFDAIQHGVLRHVVKPKPGPRQDNLVNIIVLWGSDVFTPMLAEL
GLEANNILTVASVDEIARASEAAASATFCYSVGGYLGAALEEQYGVQEIKAPQPYGFDGTDAWLRALAKATGREERAEAY
IAREHARVRPQIERLRERLKGVRGYVAMGAAYAHGVIGVLRELGVEVPGSLVFHHDPVYDSHDVRQDTLGHMIDAYGDVE
HFSISNRQPYQFYNLLKRSDPDFIIIRHGGLAGLASRLGVPAIALGDASTAIGYQGMIDLGEEIVDALAQRKFHQDLAGH
TSLPYKQWWLDQADPYLLARQNAPQAAGSRV

Sequences:

>Translated_511_residues
MAINLRSPSAETREQRLGTITSWDGPASRLANESAFSRGDCGPGCGGKGQRICELESPFSQGSTCSEQIAESQASNVRDA
VLVQHSPIGCAAGHINSNAFFRNGLMRRGLAPRNVTGLSSNLIERDMIYGGAEKLRATIKAAVERHQPKAVFVATSCATG
IIGDDVESVVRDCEDELGVPVVAMYCEGFKSKHWSSGFDAIQHGVLRHVVKPKPGPRQDNLVNIIVLWGSDVFTPMLAEL
GLEANNILTVASVDEIARASEAAASATFCYSVGGYLGAALEEQYGVQEIKAPQPYGFDGTDAWLRALAKATGREERAEAY
IAREHARVRPQIERLRERLKGVRGYVAMGAAYAHGVIGVLRELGVEVPGSLVFHHDPVYDSHDVRQDTLGHMIDAYGDVE
HFSISNRQPYQFYNLLKRSDPDFIIIRHGGLAGLASRLGVPAIALGDASTAIGYQGMIDLGEEIVDALAQRKFHQDLAGH
TSLPYKQWWLDQADPYLLARQNAPQAAGSRV
>Mature_510_residues
AINLRSPSAETREQRLGTITSWDGPASRLANESAFSRGDCGPGCGGKGQRICELESPFSQGSTCSEQIAESQASNVRDAV
LVQHSPIGCAAGHINSNAFFRNGLMRRGLAPRNVTGLSSNLIERDMIYGGAEKLRATIKAAVERHQPKAVFVATSCATGI
IGDDVESVVRDCEDELGVPVVAMYCEGFKSKHWSSGFDAIQHGVLRHVVKPKPGPRQDNLVNIIVLWGSDVFTPMLAELG
LEANNILTVASVDEIARASEAAASATFCYSVGGYLGAALEEQYGVQEIKAPQPYGFDGTDAWLRALAKATGREERAEAYI
AREHARVRPQIERLRERLKGVRGYVAMGAAYAHGVIGVLRELGVEVPGSLVFHHDPVYDSHDVRQDTLGHMIDAYGDVEH
FSISNRQPYQFYNLLKRSDPDFIIIRHGGLAGLASRLGVPAIALGDASTAIGYQGMIDLGEEIVDALAQRKFHQDLAGHT
SLPYKQWWLDQADPYLLARQNAPQAAGSRV

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: 1.18.6.1

Molecular weight: Translated: 55375; Mature: 55244

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINLRSPSAETREQRLGTITSWDGPASRLANESAFSRGDCGPGCGGKGQRICELESPFS
CEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCC
QGSTCSEQIAESQASNVRDAVLVQHSPIGCAAGHINSNAFFRNGLMRRGLAPRNVTGLSS
CCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHCCCCCCCCCCCHH
NLIERDMIYGGAEKLRATIKAAVERHQPKAVFVATSCATGIIGDDVESVVRDCEDELGVP
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCC
VVAMYCEGFKSKHWSSGFDAIQHGVLRHVVKPKPGPRQDNLVNIIVLWGSDVFTPMLAEL
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHH
GLEANNILTVASVDEIARASEAAASATFCYSVGGYLGAALEEQYGVQEIKAPQPYGFDGT
CCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCH
DAWLRALAKATGREERAEAYIAREHARVRPQIERLRERLKGVRGYVAMGAAYAHGVIGVL
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELGVEVPGSLVFHHDPVYDSHDVRQDTLGHMIDAYGDVEHFSISNRQPYQFYNLLKRSD
HHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCC
PDFIIIRHGGLAGLASRLGVPAIALGDASTAIGYQGMIDLGEEIVDALAQRKFHQDLAGH
CCEEEEECCCHHHHHHHCCCCEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
TSLPYKQWWLDQADPYLLARQNAPQAAGSRV
CCCCHHHHHCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AINLRSPSAETREQRLGTITSWDGPASRLANESAFSRGDCGPGCGGKGQRICELESPFS
EECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCC
QGSTCSEQIAESQASNVRDAVLVQHSPIGCAAGHINSNAFFRNGLMRRGLAPRNVTGLSS
CCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHCCCCCCCCCCCHH
NLIERDMIYGGAEKLRATIKAAVERHQPKAVFVATSCATGIIGDDVESVVRDCEDELGVP
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCC
VVAMYCEGFKSKHWSSGFDAIQHGVLRHVVKPKPGPRQDNLVNIIVLWGSDVFTPMLAEL
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHH
GLEANNILTVASVDEIARASEAAASATFCYSVGGYLGAALEEQYGVQEIKAPQPYGFDGT
CCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCH
DAWLRALAKATGREERAEAYIAREHARVRPQIERLRERLKGVRGYVAMGAAYAHGVIGVL
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELGVEVPGSLVFHHDPVYDSHDVRQDTLGHMIDAYGDVEHFSISNRQPYQFYNLLKRSD
HHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCC
PDFIIIRHGGLAGLASRLGVPAIALGDASTAIGYQGMIDLGEEIVDALAQRKFHQDLAGH
CCEEEEECCCHHHHHHHCCCCEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
TSLPYKQWWLDQADPYLLARQNAPQAAGSRV
CCCCHHHHHCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; H+; N2; ATP

Specific reaction: 3 reduced ferredoxin + 6 H+ + N2 + n ATP = 3 oxidized ferredoxin + 2 NH3 + n ADP + n phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309 [H]