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The map label for this gene is shiA [H]

Identifier: 192290969

GI number: 192290969

Start: 2795057

End: 2796364

Strand: Reverse

Name: shiA [H]

Synonym: Rpal_2586

Alternate gene names: 192290969

Gene position: 2796364-2795057 (Counterclockwise)

Preceding gene: 192290971

Following gene: 192290968

Centisome position: 48.68

GC content: 63.84

Gene sequence:

>1308_bases
ATGGCTGAGGTTTTACAGGACCAACGCGCGGTCGGCGGAAACCAGAAATCCCGCCGAGCGGCGTGGGCCGCTCTGATCGG
CACGGTGCTGGAGTGGTACGACTTCATCATCTACGGAACGGCCGCAGCCGTCGTCCTGAATAAGGTCTTCTTCCCCTCGA
CTGACCCGTACGTCGGAACGCTCGCCGTGTTTGCGACCTACGCGGTCGGCTTCCTCGCCCGCCCCTTGGGCGGCCTGTAT
CTCGGCTACCTCGGCGACCGATACGGCAGAAAGCGCGTGCTCGTGCTCACGCTCGTGATCATGGGCGCGGCCACGTTCCT
GCTGGGCTGTCTGCCGTCCTACGCGCAAATTGGAATCTGGGCGCCGATCCTGCTTGTCGGATTGCGCCTGCTGCAGGGAT
TCGGCGCAGGCGGTGAATACGCCGGCGCGGTGGTGCTGTCCGTCGAACACGCTCAGGAGGGCAAGCGCGGCGCTGCAGGC
GCCTGGGCTCCGATGGGCTTTGCGCTGGCAACCTTGCTCGCCAACGCCGTGTTCTTCTCCGTCATTGCCTCGATGCCCGA
AGACCAGTTCCTGCAGTGGGGTTGGCGCATTCCCTTCCTGTTCGGCGGCGTGTGCGTGCTGGTCGGCTACTTCATTCGCC
GCGGCGTCGAGGAGCCGCCGGTCTATGAACGTGCCGTCGAGCAGGCGAAGACCGAGAAGATCGGCCTGTTCACGTCGATC
AAACGCAACCCGCGCAGCTTCGTCATCGTGCTTCTGACGCGGATGGGAGAGAACGGCTTTGCCTATCTCTATCCGGTGTT
CTGCGTCTCCTATGTGATCACGACGCTCGGGCAGCCACGCTCGACCGCGCTGTGGGCCGTCATTATCGCATCGATGGTCC
AGGTCGCCACCATCCCGTTCTTCGGCGTGCTGTCCGACCGGATTGGCCGCAAGACGGTGTATATTGCGGGCACGCTGCTT
TCGATGGCGTGGCTGGTGCCGTTCTTCCTGCTGCTCGACACCGGCGCCACCGCCGCCATCATCCTGGGGTTTGGCATCGG
CCTCGGCATTGCGTATCCGGCGATGCTTGCGCCGCAGGCTTCCTGGTACGCCGAACTGTTCGACACCCAGTTCCGGCTCT
CAGGCTTCGCCTTCTCCCGGGAGCTCGGCTCATTGCTCGCCGGCGGCGTCGCTCCATTCATCGCCACGGCGCTCGTCGCA
TGGGCCCATCATTGGTGGCCGATCGTCGCCTACATGTGGGCGCTCGGCATCATCACTTTGATCGGCCTTAAGCTTGGGCC
GGAAACCGTCAGCAAGAAACTCGGATAA

Upstream 100 bases:

>100_bases
GCCACTGGACTCTACTGCCATACCTGTAATATATCAGCATATATCATCGATATATCAATGGTCGATGAGGCTATAATTCG
TTGAAAACGGGGAAACATCC

Downstream 100 bases:

>100_bases
GTGGAAGAGCGTTCCGGCGCGCCGAGTCGGAACGCTCATTGCTCCCGCATCTTTGGAGATTGGCCGATGAACGAACTCAG
AAGCAACTTCGAGCCGGGTT

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAEVLQDQRAVGGNQKSRRAAWAALIGTVLEWYDFIIYGTAAAVVLNKVFFPSTDPYVGTLAVFATYAVGFLARPLGGLY
LGYLGDRYGRKRVLVLTLVIMGAATFLLGCLPSYAQIGIWAPILLVGLRLLQGFGAGGEYAGAVVLSVEHAQEGKRGAAG
AWAPMGFALATLLANAVFFSVIASMPEDQFLQWGWRIPFLFGGVCVLVGYFIRRGVEEPPVYERAVEQAKTEKIGLFTSI
KRNPRSFVIVLLTRMGENGFAYLYPVFCVSYVITTLGQPRSTALWAVIIASMVQVATIPFFGVLSDRIGRKTVYIAGTLL
SMAWLVPFFLLLDTGATAAIILGFGIGLGIAYPAMLAPQASWYAELFDTQFRLSGFAFSRELGSLLAGGVAPFIATALVA
WAHHWWPIVAYMWALGIITLIGLKLGPETVSKKLG

Sequences:

>Translated_435_residues
MAEVLQDQRAVGGNQKSRRAAWAALIGTVLEWYDFIIYGTAAAVVLNKVFFPSTDPYVGTLAVFATYAVGFLARPLGGLY
LGYLGDRYGRKRVLVLTLVIMGAATFLLGCLPSYAQIGIWAPILLVGLRLLQGFGAGGEYAGAVVLSVEHAQEGKRGAAG
AWAPMGFALATLLANAVFFSVIASMPEDQFLQWGWRIPFLFGGVCVLVGYFIRRGVEEPPVYERAVEQAKTEKIGLFTSI
KRNPRSFVIVLLTRMGENGFAYLYPVFCVSYVITTLGQPRSTALWAVIIASMVQVATIPFFGVLSDRIGRKTVYIAGTLL
SMAWLVPFFLLLDTGATAAIILGFGIGLGIAYPAMLAPQASWYAELFDTQFRLSGFAFSRELGSLLAGGVAPFIATALVA
WAHHWWPIVAYMWALGIITLIGLKLGPETVSKKLG
>Mature_434_residues
AEVLQDQRAVGGNQKSRRAAWAALIGTVLEWYDFIIYGTAAAVVLNKVFFPSTDPYVGTLAVFATYAVGFLARPLGGLYL
GYLGDRYGRKRVLVLTLVIMGAATFLLGCLPSYAQIGIWAPILLVGLRLLQGFGAGGEYAGAVVLSVEHAQEGKRGAAGA
WAPMGFALATLLANAVFFSVIASMPEDQFLQWGWRIPFLFGGVCVLVGYFIRRGVEEPPVYERAVEQAKTEKIGLFTSIK
RNPRSFVIVLLTRMGENGFAYLYPVFCVSYVITTLGQPRSTALWAVIIASMVQVATIPFFGVLSDRIGRKTVYIAGTLLS
MAWLVPFFLLLDTGATAAIILGFGIGLGIAYPAMLAPQASWYAELFDTQFRLSGFAFSRELGSLLAGGVAPFIATALVAW
AHHWWPIVAYMWALGIITLIGLKLGPETVSKKLG

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=410, Percent_Identity=40.7317073170732, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1790550, Length=431, Percent_Identity=34.5707656612529, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789941, Length=441, Percent_Identity=34.6938775510204, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788942, Length=403, Percent_Identity=31.0173697270471, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87082404, Length=398, Percent_Identity=25.6281407035176, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082231, Length=443, Percent_Identity=23.2505643340858, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47052; Mature: 46921

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCHHHHHHCCC
>Mature Secondary Structure 
AEVLQDQRAVGGNQKSRRAAWAALIGTVLEWYDFIIYGTAAAVVLNKVFFPSTDPYVGT
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH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HHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]