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The map label for this gene is ilvD [H]

Identifier: 192290968

GI number: 192290968

Start: 2793311

End: 2794990

Strand: Reverse

Name: ilvD [H]

Synonym: Rpal_2585

Alternate gene names: 192290968

Gene position: 2794990-2793311 (Counterclockwise)

Preceding gene: 192290969

Following gene: 192290967

Centisome position: 48.66

GC content: 63.45

Gene sequence:

>1680_bases
ATGAACGAACTCAGAAGCAACTTCGAGCCGGGTTCGACCCGGTGGGCCGTGCGTCGCGCGCAATGGCTGGCGATGGGCAT
CAAGGAGGAGGATTTCCACAAGCCCAAGATCGCGGTGGTCAATTCATCCAGCGGTCTCTCGGTCTGTTACCAGCACCTGG
ACGATATCTCCCGGAAGGTGCAGCAGGCGATCCGCGATGCAGGTGGTCTCCCATTTGAAATCCGCACCGTTGCCCCGTCT
GATTTCGTCACCTCCGCAGGCCGCAAGGCGCGCTATCTGATGCCGTCTCGCGATCTCCTTGTGAACGACGTCGAGGTCCA
GGTCGAAGGCGCGGAATTGGACGGCATGGTTCTACTCGCCTCCTGCGACAAGACCACGCCCGGCCAGTTGATGGCCGCTG
GTCGCTTGAACATCCCGTCGATCGTCCTGGCCTGCGGATATCAGCTCGGCGGCCATTGCGGCGGCAAAGACGTCGATATC
GAAGACCTCTACAACGGAGTCGGCACCTACAAATCGGGCCAGATCAGCTTGCAGGATCTGACCGACATGTCCTGCGCCGC
AATCAAGGGACCAGGCGTTTGCGCCGGTCTCGCAACCGCCAATTCCATGCACTGCCTCGCAGAGGCACTCGGCATGGCCC
TGCCCGGCAATGCGCCGATCCGCGCTGGAGGCGAGCGGCTGTCCGACTACGCGGTGCAGGTCGGCCAGGCCATCGTGCGG
CTCATTCAGCAAGACCTGAGGCCGCGGCAGATCCTGACCAAGGCGTCGATCCAGAACGGAATTCGTATCGCGCAAGCGCT
GGGGCTTTCGGTCAACACGGTGCGGCATCTGACGGCGATCGCCATCGAAAGCCGCATCGAAATGGACGTCGTGGCAGAAT
ACGAGGCTCTGTCGAAGGACGCGGTTCAGATCACGCAAGTGCGTCCCAACGGTCCGCATCGGATCGAGATGCTGGACGCT
GCAGGTGGCGTGCGAGGCGCAATGAAGCAACTCGCCAGCAAACTCGATCTCTCCACGATGACGGTCACCGGAAAGACGCT
CGAGGAAAATCTCGCCGAGGCGCCCGCACCGGACGAACGCGTGATCCGGCCGCTGTCCAACCCGTTCCGCAACGAGCCCG
GGCTCATCATCATTCGCGGCAATCTCGCGCCGGACGGCGCGGTGGTGAAGCTGTCCGCCGTCCCGACCTCCGTCCGCCGG
TTCGAGGGCGTGGCGAAGGTTTTCGAAGACGAGGATAAGGCGATCGCCGGCCTCAGCGATGGCTCAATTCACAAGGGACA
GGCCATCATCCTCCGGCACATGGGCCCGAAGGGCGGCCCGGGCACGGTGTTCGCGTGCTCGTTCATGGCGGCTCTGGTCG
GCGCCGGCCTGTCGAGCGGCGTCGCGGTTCTGACCGACGGTGAGTTGTCGGGTCTCAACTCCGGCATCACTGTTGGCCAG
ATCATGCCGGAGGCTGCCGAAGGTGGCCCGCTCGCCGTGGTGGCTGACGGCGACCGTATCGCGATCGACCTGACCGCCGA
AACAATCGAGCTGATGATCACCGAAAAGGAACTCGCGACCAGACTAGCCGAATGGAAACCGCGGACGTTGCCGGCCGAAT
ATGGCTGGCTCGCGCAATACGCCCAGCTCGTCCAGCCGCTCTCGAAGGGAGCCGTACTCGGCGATCGAAGCCGTCGCTGA

Upstream 100 bases:

>100_bases
TGGGCCGGAAACCGTCAGCAAGAAACTCGGATAAGTGGAAGAGCGTTCCGGCGCGCCGAGTCGGAACGCTCATTGCTCCC
GCATCTTTGGAGATTGGCCG

Downstream 100 bases:

>100_bases
TAGCGCAGCCGCAGCCGCGCCCCCGAGCCGCTAGTCATAAGCGAGGTTATTTTGATGGACTCCACATGCGTCAACATCCC
GGCTTGGGAAATCCGGCACC

Product: Dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MNELRSNFEPGSTRWAVRRAQWLAMGIKEEDFHKPKIAVVNSSSGLSVCYQHLDDISRKVQQAIRDAGGLPFEIRTVAPS
DFVTSAGRKARYLMPSRDLLVNDVEVQVEGAELDGMVLLASCDKTTPGQLMAAGRLNIPSIVLACGYQLGGHCGGKDVDI
EDLYNGVGTYKSGQISLQDLTDMSCAAIKGPGVCAGLATANSMHCLAEALGMALPGNAPIRAGGERLSDYAVQVGQAIVR
LIQQDLRPRQILTKASIQNGIRIAQALGLSVNTVRHLTAIAIESRIEMDVVAEYEALSKDAVQITQVRPNGPHRIEMLDA
AGGVRGAMKQLASKLDLSTMTVTGKTLEENLAEAPAPDERVIRPLSNPFRNEPGLIIIRGNLAPDGAVVKLSAVPTSVRR
FEGVAKVFEDEDKAIAGLSDGSIHKGQAIILRHMGPKGGPGTVFACSFMAALVGAGLSSGVAVLTDGELSGLNSGITVGQ
IMPEAAEGGPLAVVADGDRIAIDLTAETIELMITEKELATRLAEWKPRTLPAEYGWLAQYAQLVQPLSKGAVLGDRSRR

Sequences:

>Translated_559_residues
MNELRSNFEPGSTRWAVRRAQWLAMGIKEEDFHKPKIAVVNSSSGLSVCYQHLDDISRKVQQAIRDAGGLPFEIRTVAPS
DFVTSAGRKARYLMPSRDLLVNDVEVQVEGAELDGMVLLASCDKTTPGQLMAAGRLNIPSIVLACGYQLGGHCGGKDVDI
EDLYNGVGTYKSGQISLQDLTDMSCAAIKGPGVCAGLATANSMHCLAEALGMALPGNAPIRAGGERLSDYAVQVGQAIVR
LIQQDLRPRQILTKASIQNGIRIAQALGLSVNTVRHLTAIAIESRIEMDVVAEYEALSKDAVQITQVRPNGPHRIEMLDA
AGGVRGAMKQLASKLDLSTMTVTGKTLEENLAEAPAPDERVIRPLSNPFRNEPGLIIIRGNLAPDGAVVKLSAVPTSVRR
FEGVAKVFEDEDKAIAGLSDGSIHKGQAIILRHMGPKGGPGTVFACSFMAALVGAGLSSGVAVLTDGELSGLNSGITVGQ
IMPEAAEGGPLAVVADGDRIAIDLTAETIELMITEKELATRLAEWKPRTLPAEYGWLAQYAQLVQPLSKGAVLGDRSRR
>Mature_559_residues
MNELRSNFEPGSTRWAVRRAQWLAMGIKEEDFHKPKIAVVNSSSGLSVCYQHLDDISRKVQQAIRDAGGLPFEIRTVAPS
DFVTSAGRKARYLMPSRDLLVNDVEVQVEGAELDGMVLLASCDKTTPGQLMAAGRLNIPSIVLACGYQLGGHCGGKDVDI
EDLYNGVGTYKSGQISLQDLTDMSCAAIKGPGVCAGLATANSMHCLAEALGMALPGNAPIRAGGERLSDYAVQVGQAIVR
LIQQDLRPRQILTKASIQNGIRIAQALGLSVNTVRHLTAIAIESRIEMDVVAEYEALSKDAVQITQVRPNGPHRIEMLDA
AGGVRGAMKQLASKLDLSTMTVTGKTLEENLAEAPAPDERVIRPLSNPFRNEPGLIIIRGNLAPDGAVVKLSAVPTSVRR
FEGVAKVFEDEDKAIAGLSDGSIHKGQAIILRHMGPKGGPGTVFACSFMAALVGAGLSSGVAVLTDGELSGLNSGITVGQ
IMPEAAEGGPLAVVADGDRIAIDLTAETIELMITEKELATRLAEWKPRTLPAEYGWLAQYAQLVQPLSKGAVLGDRSRR

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=618, Percent_Identity=32.8478964401294, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI2367371, Length=514, Percent_Identity=28.2101167315175, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788157, Length=517, Percent_Identity=27.0793036750484, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1786464, Length=487, Percent_Identity=27.5154004106776, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322476, Length=548, Percent_Identity=35.7664233576642, Blast_Score=316, Evalue=7e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59335; Mature: 59335

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELRSNFEPGSTRWAVRRAQWLAMGIKEEDFHKPKIAVVNSSSGLSVCYQHLDDISRKV
CCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
QQAIRDAGGLPFEIRTVAPSDFVTSAGRKARYLMPSRDLLVNDVEVQVEGAELDGMVLLA
HHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEECCCCCEEEEEEEEEEECCCCCCEEEEE
SCDKTTPGQLMAAGRLNIPSIVLACGYQLGGHCGGKDVDIEDLYNGVGTYKSGQISLQDL
ECCCCCCCCEEECCCCCCCHHHHHHCHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHH
TDMSCAAIKGPGVCAGLATANSMHCLAEALGMALPGNAPIRAGGERLSDYAVQVGQAIVR
CCCCHHEECCCCCEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LIQQDLRPRQILTKASIQNGIRIAQALGLSVNTVRHLTAIAIESRIEMDVVAEYEALSKD
HHHHHCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
AVQITQVRPNGPHRIEMLDAAGGVRGAMKQLASKLDLSTMTVTGKTLEENLAEAPAPDER
CEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCHHH
VIRPLSNPFRNEPGLIIIRGNLAPDGAVVKLSAVPTSVRRFEGVAKVFEDEDKAIAGLSD
HHHHHCCCCCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCC
GSIHKGQAIILRHMGPKGGPGTVFACSFMAALVGAGLSSGVAVLTDGELSGLNSGITVGQ
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHH
IMPEAAEGGPLAVVADGDRIAIDLTAETIELMITEKELATRLAEWKPRTLPAEYGWLAQY
CCCCCCCCCCEEEEECCCEEEEEEEEEEEEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHH
AQLVQPLSKGAVLGDRSRR
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNELRSNFEPGSTRWAVRRAQWLAMGIKEEDFHKPKIAVVNSSSGLSVCYQHLDDISRKV
CCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
QQAIRDAGGLPFEIRTVAPSDFVTSAGRKARYLMPSRDLLVNDVEVQVEGAELDGMVLLA
HHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEECCCCCEEEEEEEEEEECCCCCCEEEEE
SCDKTTPGQLMAAGRLNIPSIVLACGYQLGGHCGGKDVDIEDLYNGVGTYKSGQISLQDL
ECCCCCCCCEEECCCCCCCHHHHHHCHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHH
TDMSCAAIKGPGVCAGLATANSMHCLAEALGMALPGNAPIRAGGERLSDYAVQVGQAIVR
CCCCHHEECCCCCEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LIQQDLRPRQILTKASIQNGIRIAQALGLSVNTVRHLTAIAIESRIEMDVVAEYEALSKD
HHHHHCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
AVQITQVRPNGPHRIEMLDAAGGVRGAMKQLASKLDLSTMTVTGKTLEENLAEAPAPDER
CEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCHHH
VIRPLSNPFRNEPGLIIIRGNLAPDGAVVKLSAVPTSVRRFEGVAKVFEDEDKAIAGLSD
HHHHHCCCCCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCC
GSIHKGQAIILRHMGPKGGPGTVFACSFMAALVGAGLSSGVAVLTDGELSGLNSGITVGQ
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHH
IMPEAAEGGPLAVVADGDRIAIDLTAETIELMITEKELATRLAEWKPRTLPAEYGWLAQY
CCCCCCCCCCEEEEECCCEEEEEEEEEEEEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHH
AQLVQPLSKGAVLGDRSRR
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA