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The map label for this gene is nodW [H]

Identifier: 192290784

GI number: 192290784

Start: 2588806

End: 2589438

Strand: Reverse

Name: nodW [H]

Synonym: Rpal_2400

Alternate gene names: 192290784

Gene position: 2589438-2588806 (Counterclockwise)

Preceding gene: 192290786

Following gene: 192290783

Centisome position: 45.08

GC content: 63.03

Gene sequence:

>633_bases
ATGGCTTCCCTTCCCAGTCCCCTGATCGTGGTCGTCGATGACGACGCCGGCATCAGAGAAGCGCTGGAAGGCCTGTTCAG
GTCCGTCGGCCATCGGGTGGCACTGTTCGGATCGGCGCAAGACTTGCTGACGACGGGTGTTCCGCTCGATGCCAACTGCC
TGATCCTCGATGTCCGCCTGCCCGGCACCAGCGGTCTGGACCTGCAGGACATGCTGTCCCGGCTGGAAGTCCGGGTCCCG
ATCATTTTCATGACCGGCTACGGCGATGTGCCGATGACGGTGCGGGGGATGAAGGCCGGTGCCGTCGACTTCCTAACCAA
GCCGTTCCGCGACCAGGAGATGCTGGACGCGGTGTCAGCGGCGGTCGAGCTCGATCGCCGGCGTCGCGAGGCGGAACACA
AGCGCAACGAGATCGTGCAGCGCTTTGATGCCCTCACCCCGCGTGAAAAGCAGATCTTCGAAATGGTCTCGGCCGGATCG
ATGAACAAGAACGTCGCCGCGGATCTCGGACTGAGCGAGATCACAGTGAAGATTCATCGCGGCCGCGTGATGAAGAAGAT
GGGCGCACGGTCACTCGCCGATTTGGTGCGAATGGCCGAAGTTCTCGCGCCGCGCGCTGGGGAAGCGACGTAA

Upstream 100 bases:

>100_bases
GCTGCCGTCAGAGCCGTCATTTGGCGCTGAAACTGGATTGTCCGCCAAAGCTTGGTTAGATTTCGGCGGCGCGGAATCGC
AGAGGACGCAGACCCTTTTG

Downstream 100 bases:

>100_bases
AACCATCGTATGACTTATATTGCAGAAACTGGTGCCGCACAGTTTCTGAGCGTCGGATCGTACTGCATCACTCTTGAGGA
TGGCAGAAATGACCATCGGC

Product: LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MASLPSPLIVVVDDDAGIREALEGLFRSVGHRVALFGSAQDLLTTGVPLDANCLILDVRLPGTSGLDLQDMLSRLEVRVP
IIFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSAAVELDRRRREAEHKRNEIVQRFDALTPREKQIFEMVSAGS
MNKNVAADLGLSEITVKIHRGRVMKKMGARSLADLVRMAEVLAPRAGEAT

Sequences:

>Translated_210_residues
MASLPSPLIVVVDDDAGIREALEGLFRSVGHRVALFGSAQDLLTTGVPLDANCLILDVRLPGTSGLDLQDMLSRLEVRVP
IIFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSAAVELDRRRREAEHKRNEIVQRFDALTPREKQIFEMVSAGS
MNKNVAADLGLSEITVKIHRGRVMKKMGARSLADLVRMAEVLAPRAGEAT
>Mature_209_residues
ASLPSPLIVVVDDDAGIREALEGLFRSVGHRVALFGSAQDLLTTGVPLDANCLILDVRLPGTSGLDLQDMLSRLEVRVPI
IFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSAAVELDRRRREAEHKRNEIVQRFDALTPREKQIFEMVSAGSM
NKNVAADLGLSEITVKIHRGRVMKKMGARSLADLVRMAEVLAPRAGEAT

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=166, Percent_Identity=32.5301204819277, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788550, Length=126, Percent_Identity=29.3650793650794, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=30.2325581395349, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788222, Length=205, Percent_Identity=25.3658536585366, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1790863, Length=111, Percent_Identity=30.6306306306306, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1790437, Length=175, Percent_Identity=29.1428571428571, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22970; Mature: 22839

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLPSPLIVVVDDDAGIREALEGLFRSVGHRVALFGSAQDLLTTGVPLDANCLILDVRL
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCCCCEEEEEEEC
PGTSGLDLQDMLSRLEVRVPIIFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSA
CCCCCCCHHHHHHHHHEEEEEEEEECCCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHH
AVELDRRRREAEHKRNEIVQRFDALTPREKQIFEMVSAGSMNKNVAADLGLSEITVKIHR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHCCHHHEEEEHHH
GRVMKKMGARSLADLVRMAEVLAPRAGEAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ASLPSPLIVVVDDDAGIREALEGLFRSVGHRVALFGSAQDLLTTGVPLDANCLILDVRL
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCCCCEEEEEEEC
PGTSGLDLQDMLSRLEVRVPIIFMTGYGDVPMTVRGMKAGAVDFLTKPFRDQEMLDAVSA
CCCCCCCHHHHHHHHHEEEEEEEEECCCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHH
AVELDRRRREAEHKRNEIVQRFDALTPREKQIFEMVSAGSMNKNVAADLGLSEITVKIHR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHCCHHHEEEEHHH
GRVMKKMGARSLADLVRMAEVLAPRAGEAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]