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The map label for this gene is hyfR [H]

Identifier: 192290786

GI number: 192290786

Start: 2590325

End: 2592250

Strand: Reverse

Name: hyfR [H]

Synonym: Rpal_2402

Alternate gene names: 192290786

Gene position: 2592250-2590325 (Counterclockwise)

Preceding gene: 192290791

Following gene: 192290784

Centisome position: 45.13

GC content: 65.58

Gene sequence:

>1926_bases
ATGGACCTCTCGTCCCCCTCTTCTTTCACCGCCACGATCGATGCGCCGCTGCGCGCCGCCGTGTTCGACAGCGCAGTCGA
AGCTGTACTGCTGGTCGACCCGACGCTCGACCAGATCGTCGACGCCAACAACGCCGCCAGTGCCCTGCTCGGCCACGATC
GCGACACTTTGCTGCAGACCAGGTTCAGCGCGTTGCACAGCGCCCAGCTTCCTGCGCTCCTGGTGTTTACCCAGGCGGTG
TTCGCCAAGGGTACCTATTGGACGCACGCGCTGACGCCGCTGCATGCCGATGGCACGCAGCTTCGATTGGAATACGCCGG
CAAGCTTCTGCCGTATCGCGGTCGCTCGCTATTGCTGTTGACGATGAGCGATCTGGAGCAGCGCCGGCGACGCTATGTCG
ACGCCGTCGCCGACGATTACATGCGCGACGGGTTGCCTGCATGGCAGCGCGTCGAGCGGGTATTTCAGGACATCGAACGC
GAGAACCAGCTGATTTTGCGGGCGGCTGGCGAAGGCATCTACGGCGTCAACGCCGAAGGAAAGACCACCTTCGTTAATCC
TGCCGCGGAGCGGATGCTGGGCTGGTCGGCCGAAGAGCTGGTCGGCAAAGAGATGCATGCCATGGTGCATCACAGCCATC
ATGACGGCCGGCACTATCCCGGCCAGCAGTGCCCAATCTACGCGGCGTTTCGCGACGGCGCGGTGCACACCGTCGACGGC
GAAGTGTTCTGGCACAAGGACGGCACACCGGTCTGGGTCGAATACACCTCCACACCGATCCACGACCGCAGCGGCGGCGT
GGTCGGCGCCGTCGTGGTGTTTCGCGATGTCAGCCAGCGCCACGAGGCCGACGAAAAGCTGCACGCTGCGCTGGCCGAAG
TCGACCGCCTGCGCGAGCGGCTGCAGCTCGAAAACGACTATCTGCAGGAAGAAATCCGGATCGAGACCAATCCGCGCGGC
ATCATCGGACAGAGCGAAGCGATCCAGACCACGCTGCGGCAGGTGAAGCTGGTGGCGCCGACCGCCGCGACCGTGCTGAT
CACCGGCGAATCCGGCACCGGCAAGGAACTGATCGCGCGCGCGATCCACGAAGCCAGTACGCGCCGCGACCGACCGCTGA
TCAGGGTCAACTGCGCCGCGATCCCGAGGGAGTTGTTCGAGAGCGAGTTCTTCGGTCACGCCCGCGGCGCCTTCACCGGT
GCGGTGCGCGACCGCATCGGGCGGTTCGAGCTCGCTGACGGCGGCACGCTGTTCCTCGACGAGGTCGGGGAGATCCCGCT
GGAGCTGCAGAGCAAACTGCTGCGCGTGCTGCAGGAGGGCAATTTCGAGCGCGTCGGTGAGGAGCGCACCCGCAATGTCG
ATGTCCGCCTGATCGCCGCCACCAACCGCGACCTGAAGCGGGAGGTGCAGCGCGGCCATTTCCGCGAGGACCTGTATTTC
CGACTCAGCGTATTTCCGATCGAGTCGGTGCCGCTACGCGATCGCCGCGAGGACATTCCGCTGCTGGCGCAGCACTTCCT
CGCCAATGAAGCACGCGAACTGAAAACCGAGCTGCGGTTGTCGCAGGGCGATGTCCGTCGCCTGATGCGCTACGACTGGC
CCGGCAATGTCCGCGAACTGCAGAACGTGATCGAGCGCGCCACCATCCTGGCGCAGAACGGACGGCTGCGGCTCGACCTG
CCGGAAACGGCCGGTAGTCATGCCTCGGCCACCAGCGGCCGTCAGAAGCCGGACGCCCGGCCCGTGGTGATGACCGTGGC
TGATCTCCGCAACCTGGAGCGCGACAACATCATCACGGCGCTACGCGCCTGCAAAGGCAAGGTGTTCGGGGACGACGGCG
CGGCGGCGATGCTCGACATGAAACCGACCACGCTTGCGTCGCGGATCAAAGCGCTGGGCATCACCTCGCCGCGCGCCCAG
GCGTGA

Upstream 100 bases:

>100_bases
GCCAGATGCGACGATATGGGCGGTTTCGGCGGATTGCTGAATACGAGATCTCGTAATATACGGAATTTCGTAGTCGAGTT
ACGAAAACTCGGAGCGTCCG

Downstream 100 bases:

>100_bases
GCCCGGCGCTTCGGACAACGCCGCGACGACGACTGGAACTTACAGCGAAGCGACCCGGGGCTCATCGTCTCGCCGCCCGG
CGGAGCTCGGCCGGTCGTGC

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MDLSSPSSFTATIDAPLRAAVFDSAVEAVLLVDPTLDQIVDANNAASALLGHDRDTLLQTRFSALHSAQLPALLVFTQAV
FAKGTYWTHALTPLHADGTQLRLEYAGKLLPYRGRSLLLLTMSDLEQRRRRYVDAVADDYMRDGLPAWQRVERVFQDIER
ENQLILRAAGEGIYGVNAEGKTTFVNPAAERMLGWSAEELVGKEMHAMVHHSHHDGRHYPGQQCPIYAAFRDGAVHTVDG
EVFWHKDGTPVWVEYTSTPIHDRSGGVVGAVVVFRDVSQRHEADEKLHAALAEVDRLRERLQLENDYLQEEIRIETNPRG
IIGQSEAIQTTLRQVKLVAPTAATVLITGESGTGKELIARAIHEASTRRDRPLIRVNCAAIPRELFESEFFGHARGAFTG
AVRDRIGRFELADGGTLFLDEVGEIPLELQSKLLRVLQEGNFERVGEERTRNVDVRLIAATNRDLKREVQRGHFREDLYF
RLSVFPIESVPLRDRREDIPLLAQHFLANEARELKTELRLSQGDVRRLMRYDWPGNVRELQNVIERATILAQNGRLRLDL
PETAGSHASATSGRQKPDARPVVMTVADLRNLERDNIITALRACKGKVFGDDGAAAMLDMKPTTLASRIKALGITSPRAQ
A

Sequences:

>Translated_641_residues
MDLSSPSSFTATIDAPLRAAVFDSAVEAVLLVDPTLDQIVDANNAASALLGHDRDTLLQTRFSALHSAQLPALLVFTQAV
FAKGTYWTHALTPLHADGTQLRLEYAGKLLPYRGRSLLLLTMSDLEQRRRRYVDAVADDYMRDGLPAWQRVERVFQDIER
ENQLILRAAGEGIYGVNAEGKTTFVNPAAERMLGWSAEELVGKEMHAMVHHSHHDGRHYPGQQCPIYAAFRDGAVHTVDG
EVFWHKDGTPVWVEYTSTPIHDRSGGVVGAVVVFRDVSQRHEADEKLHAALAEVDRLRERLQLENDYLQEEIRIETNPRG
IIGQSEAIQTTLRQVKLVAPTAATVLITGESGTGKELIARAIHEASTRRDRPLIRVNCAAIPRELFESEFFGHARGAFTG
AVRDRIGRFELADGGTLFLDEVGEIPLELQSKLLRVLQEGNFERVGEERTRNVDVRLIAATNRDLKREVQRGHFREDLYF
RLSVFPIESVPLRDRREDIPLLAQHFLANEARELKTELRLSQGDVRRLMRYDWPGNVRELQNVIERATILAQNGRLRLDL
PETAGSHASATSGRQKPDARPVVMTVADLRNLERDNIITALRACKGKVFGDDGAAAMLDMKPTTLASRIKALGITSPRAQ
A
>Mature_641_residues
MDLSSPSSFTATIDAPLRAAVFDSAVEAVLLVDPTLDQIVDANNAASALLGHDRDTLLQTRFSALHSAQLPALLVFTQAV
FAKGTYWTHALTPLHADGTQLRLEYAGKLLPYRGRSLLLLTMSDLEQRRRRYVDAVADDYMRDGLPAWQRVERVFQDIER
ENQLILRAAGEGIYGVNAEGKTTFVNPAAERMLGWSAEELVGKEMHAMVHHSHHDGRHYPGQQCPIYAAFRDGAVHTVDG
EVFWHKDGTPVWVEYTSTPIHDRSGGVVGAVVVFRDVSQRHEADEKLHAALAEVDRLRERLQLENDYLQEEIRIETNPRG
IIGQSEAIQTTLRQVKLVAPTAATVLITGESGTGKELIARAIHEASTRRDRPLIRVNCAAIPRELFESEFFGHARGAFTG
AVRDRIGRFELADGGTLFLDEVGEIPLELQSKLLRVLQEGNFERVGEERTRNVDVRLIAATNRDLKREVQRGHFREDLYF
RLSVFPIESVPLRDRREDIPLLAQHFLANEARELKTELRLSQGDVRRLMRYDWPGNVRELQNVIERATILAQNGRLRLDL
PETAGSHASATSGRQKPDARPVVMTVADLRNLERDNIITALRACKGKVFGDDGAAAMLDMKPTTLASRIKALGITSPRAQ
A

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=343, Percent_Identity=51.8950437317784, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1789087, Length=347, Percent_Identity=51.5850144092219, Blast_Score=315, Evalue=7e-87,
Organism=Escherichia coli, GI1790437, Length=290, Percent_Identity=49.3103448275862, Blast_Score=258, Evalue=1e-69,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=45.4819277108434, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=52.9661016949153, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1788550, Length=231, Percent_Identity=54.5454545454545, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788905, Length=235, Percent_Identity=51.4893617021277, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1789233, Length=238, Percent_Identity=44.1176470588235, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87081872, Length=233, Percent_Identity=48.068669527897, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=45.4918032786885, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787583, Length=228, Percent_Identity=44.7368421052632, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87081858, Length=296, Percent_Identity=35.8108108108108, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1789828, Length=253, Percent_Identity=35.9683794466403, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 71624; Mature: 71624

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSSPSSFTATIDAPLRAAVFDSAVEAVLLVDPTLDQIVDANNAASALLGHDRDTLLQT
CCCCCCCCEEEEECCCHHHHHHHHHHCEEEEECCCHHHHHCCCCHHHHHHCCCHHHHHHH
RFSALHSAQLPALLVFTQAVFAKGTYWTHALTPLHADGTQLRLEYAGKLLPYRGRSLLLL
HHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCEEECCCCEEEEECCCCCCCCCCCEEEEE
TMSDLEQRRRRYVDAVADDYMRDGLPAWQRVERVFQDIERENQLILRAAGEGIYGVNAEG
EHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCC
KTTFVNPAAERMLGWSAEELVGKEMHAMVHHSHHDGRHYPGQQCPIYAAFRDGAVHTVDG
CEEEECHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEECC
EVFWHKDGTPVWVEYTSTPIHDRSGGVVGAVVVFRDVSQRHEADEKLHAALAEVDRLRER
EEEEECCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLENDYLQEEIRIETNPRGIIGQSEAIQTTLRQVKLVAPTAATVLITGESGTGKELIAR
HHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEECCCCCCHHHHHH
AIHEASTRRDRPLIRVNCAAIPRELFESEFFGHARGAFTGAVRDRIGRFELADGGTLFLD
HHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCEEEEC
EVGEIPLELQSKLLRVLQEGNFERVGEERTRNVDVRLIAATNRDLKREVQRGHFREDLYF
CCCCCCHHHHHHHHHHHHCCCHHHHCHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCEEE
RLSVFPIESVPLRDRREDIPLLAQHFLANEARELKTELRLSQGDVRRLMRYDWPGNVREL
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHH
QNVIERATILAQNGRLRLDLPETAGSHASATSGRQKPDARPVVMTVADLRNLERDNIITA
HHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCHHHHHHH
LRACKGKVFGDDGAAAMLDMKPTTLASRIKALGITSPRAQA
HHHHCCCEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDLSSPSSFTATIDAPLRAAVFDSAVEAVLLVDPTLDQIVDANNAASALLGHDRDTLLQT
CCCCCCCCEEEEECCCHHHHHHHHHHCEEEEECCCHHHHHCCCCHHHHHHCCCHHHHHHH
RFSALHSAQLPALLVFTQAVFAKGTYWTHALTPLHADGTQLRLEYAGKLLPYRGRSLLLL
HHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCEEECCCCEEEEECCCCCCCCCCCEEEEE
TMSDLEQRRRRYVDAVADDYMRDGLPAWQRVERVFQDIERENQLILRAAGEGIYGVNAEG
EHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCC
KTTFVNPAAERMLGWSAEELVGKEMHAMVHHSHHDGRHYPGQQCPIYAAFRDGAVHTVDG
CEEEECHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEECC
EVFWHKDGTPVWVEYTSTPIHDRSGGVVGAVVVFRDVSQRHEADEKLHAALAEVDRLRER
EEEEECCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLENDYLQEEIRIETNPRGIIGQSEAIQTTLRQVKLVAPTAATVLITGESGTGKELIAR
HHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEECCCCCCHHHHHH
AIHEASTRRDRPLIRVNCAAIPRELFESEFFGHARGAFTGAVRDRIGRFELADGGTLFLD
HHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCEEEEC
EVGEIPLELQSKLLRVLQEGNFERVGEERTRNVDVRLIAATNRDLKREVQRGHFREDLYF
CCCCCCHHHHHHHHHHHHCCCHHHHCHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCEEE
RLSVFPIESVPLRDRREDIPLLAQHFLANEARELKTELRLSQGDVRRLMRYDWPGNVREL
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHH
QNVIERATILAQNGRLRLDLPETAGSHASATSGRQKPDARPVVMTVADLRNLERDNIITA
HHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCHHHHHHH
LRACKGKVFGDDGAAAMLDMKPTTLASRIKALGITSPRAQA
HHHHCCCEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]