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The map label for this gene is cysG [H]

Identifier: 192290756

GI number: 192290756

Start: 2557122

End: 2557871

Strand: Reverse

Name: cysG [H]

Synonym: Rpal_2372

Alternate gene names: 192290756

Gene position: 2557871-2557122 (Counterclockwise)

Preceding gene: 192290757

Following gene: 192290754

Centisome position: 44.53

GC content: 70.13

Gene sequence:

>750_bases
ATGTCCAAGTTGATTGAAAGCGCTCCGGCAGCCGGCCGCATCACGCTGGTCGGCGCCGGCCCGGGTGATGCCGACCTGCT
GACGCTGCGCGCGATCAAGCGCCTGCAGGCCGCCGACGTCGTGCTGTATGACGACCTCGCCGGCGAAGACTTCATGGCCT
ATCTGCATCCCGATGCCGAGCTCGTCGCGGTCGGCAAGCGCGCCGGCCATCCCTCGCCGCGCCAGGACGAAGTCAGCCGC
CTCGCGATCAGCTACGCGCTCCGCGGCAAGCATGTGGTGCGGCTGAAGGCCGGCGATCCGACGCTGTTCGCCCGCGCCGA
CGAGGAGCTGCGCGCCGCGCGCGATGCCGGCATCGCCGTCGAGATCGTCCCCGGTATCACCACCGCGACTGCAGCCGCAG
CCGCGATCGGCGCGTCGCTGACCAAGCGCGGCGTCGCGCGCCGGGTGCAGTTCGTCACCGGCCATGATGTCGACGGCCGC
CTGCCGCGCGATCTCGATCTCGCAGCGCTGGCCGACGCGACGGCCACGACCTGCGTCTATATGGGCAAGGCCACCTTCCC
CGCCCTTGTGGCGCAACTGATCGCACGCGGCCTGAGCGCGGCGACGCCGGCCGTGATGGTCGAAAGCCTCGGCACAGACG
CGACCAACGTGCAGCGAGGCAGCGTCAGCGAGATCGCCGCCCGCCTTACCGCCGATCGGCCATCCGGCCCCTGCATGATC
CTGTACGGTGCTGCATTGGAAGAGAGCTGA

Upstream 100 bases:

>100_bases
ATCCCGCCGGCCAACTTACCGATGAGACCGGCAGCCGCCGCGGGCACGTCACCGGCACGTTCTTCCATCTGATCGATGTC
GCCGAGGAGGCGATCGCCTG

Downstream 100 bases:

>100_bases
GCGCCAGCGCGCGACCGGCGATGACGACTCAGCTAACTACCGCGTCTCGGCGGCCGCAACGCCTCGCCTCTGGCTGATCT
GCTCGATCGCATCGGATAGC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSKLIESAPAAGRITLVGAGPGDADLLTLRAIKRLQAADVVLYDDLAGEDFMAYLHPDAELVAVGKRAGHPSPRQDEVSR
LAISYALRGKHVVRLKAGDPTLFARADEELRAARDAGIAVEIVPGITTATAAAAAIGASLTKRGVARRVQFVTGHDVDGR
LPRDLDLAALADATATTCVYMGKATFPALVAQLIARGLSAATPAVMVESLGTDATNVQRGSVSEIAARLTADRPSGPCMI
LYGAALEES

Sequences:

>Translated_249_residues
MSKLIESAPAAGRITLVGAGPGDADLLTLRAIKRLQAADVVLYDDLAGEDFMAYLHPDAELVAVGKRAGHPSPRQDEVSR
LAISYALRGKHVVRLKAGDPTLFARADEELRAARDAGIAVEIVPGITTATAAAAAIGASLTKRGVARRVQFVTGHDVDGR
LPRDLDLAALADATATTCVYMGKATFPALVAQLIARGLSAATPAVMVESLGTDATNVQRGSVSEIAARLTADRPSGPCMI
LYGAALEES
>Mature_248_residues
SKLIESAPAAGRITLVGAGPGDADLLTLRAIKRLQAADVVLYDDLAGEDFMAYLHPDAELVAVGKRAGHPSPRQDEVSRL
AISYALRGKHVVRLKAGDPTLFARADEELRAARDAGIAVEIVPGITTATAAAAAIGASLTKRGVARRVQFVTGHDVDGRL
PRDLDLAALADATATTCVYMGKATFPALVAQLIARGLSAATPAVMVESLGTDATNVQRGSVSEIAARLTADRPSGPCMIL
YGAALEES

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=233, Percent_Identity=40.343347639485, Blast_Score=159, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322922, Length=203, Percent_Identity=30.0492610837438, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 25822; Mature: 25691

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLIESAPAAGRITLVGAGPGDADLLTLRAIKRLQAADVVLYDDLAGEDFMAYLHPDAE
CCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCE
LVAVGKRAGHPSPRQDEVSRLAISYALRGKHVVRLKAGDPTLFARADEELRAARDAGIAV
EEEECHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHHHHCCCEEE
EIVPGITTATAAAAAIGASLTKRGVARRVQFVTGHDVDGRLPRDLDLAALADATATTCVY
EEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHCCHHHEEEE
MGKATFPALVAQLIARGLSAATPAVMVESLGTDATNVQRGSVSEIAARLTADRPSGPCMI
ECCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEE
LYGAALEES
EEECCCCCC
>Mature Secondary Structure 
SKLIESAPAAGRITLVGAGPGDADLLTLRAIKRLQAADVVLYDDLAGEDFMAYLHPDAE
CCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCE
LVAVGKRAGHPSPRQDEVSRLAISYALRGKHVVRLKAGDPTLFARADEELRAARDAGIAV
EEEECHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHHHHCCCEEE
EIVPGITTATAAAAAIGASLTKRGVARRVQFVTGHDVDGRLPRDLDLAALADATATTCVY
EEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHCCHHHEEEE
MGKATFPALVAQLIARGLSAATPAVMVESLGTDATNVQRGSVSEIAARLTADRPSGPCMI
ECCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEE
LYGAALEES
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]