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The map label for this gene is cobB [H]

Identifier: 192290757

GI number: 192290757

Start: 2557871

End: 2559232

Strand: Reverse

Name: cobB [H]

Synonym: Rpal_2373

Alternate gene names: 192290757

Gene position: 2559232-2557871 (Counterclockwise)

Preceding gene: 192290758

Following gene: 192290756

Centisome position: 44.55

GC content: 69.24

Gene sequence:

>1362_bases
GTGATCGTGACTGATCACATCCTCCCAACAGCGCCGAACGGCCGACCGCCTGGGCTGATCATCGCGGCGCCATCGACCAA
TAGCGGCAAGACCACGGTGACGCTGGCGCTGCACGCGGCGCTGCGCCGCCGCAGCCTCACCGTGCAGCCCTACAAATGCG
GCCCCGACTATATCGATCCGGCGTTCCACGAGATCGCGGCCGGCCGGCCCTGCTACAATCTGGATTCCTGGGCGTTCGGC
CAGGACCGCATCACCTCCCTGCTCGATGCCGCGGCCGGCGCCGATCTCTGCCTCGCCGAAGGCGTGATGGGCCTGTTCGA
CGGCGGCAGCGCCCGTGGCGCATGGGGTAACGGCGCGACCGCCGATATCGCCGCGTTCACCGGATGGCCGGTAGTGCTGG
TGATCGACGTCGCCGGCCAGGCGCAGTCGGCGGCCGCGCTGGCGCTCGGCTTCGCCCGCTATCGCAGCGATGTCCGCATC
GCCGGCGTCATTCTCAACAACGTTGCCAGCACACGCCATGAAGCGCTGGTCCGCGATGGCTTCGCCGCGCAGGATATCCC
GGTGCTCGGAGGCTTCGAGCGGCAGCGCGACCTCACGCTGAAGAACCGCCATCTCGGCCTGGTGCAGGCGCGCGAGACCG
CGAGACTCGACGTCCGTCTGCAGCAACTCGCTGATAGTGCCGTGCGCGGTATCGATCTCGACGCGCTGATCCGCGCCGCG
CAACCGGCGCGCCCGATCGCATCGACGATCCGGCTGCTGACGCCACCCGGCCAGCGTATCGCGCTGGCGCATGACGATGC
GTTCTCGTTCATCTATCCGCACCTCACCGCCGGCTGGCGTGCCGCCGGAGCTGAGATCGTGCCGTTCTCGCCGCTGAACG
ATGAAGGCCCCGATCCATCCTGCGACGCCGCGTGGCTGCCGGGCGGCTATCCCGAACTCCACGCCGGTCGGCTCGCGGCC
GCCACGCGGTTCAAGGCGGCGCTCGGCGCTTTTGCCGAGACACGCCCGGTGCACGGCGAGTGCGGCGGCTACATGACGCT
GGGCGCCGGCCTGATCGACGCCGACGGCACGCGCCATGCGATGGCTGGACTGCTCGGCCTCGAAACCGACTTCGCCAGTC
GCAAGCTGCATCTCGGCTACCGCCTCGCGACCTTGCATGCTGACGCCGCCGGCAAAGCCGCGGGCAGTGTGCTGCGCGGC
CACGAATATCACTATGCGCGCGTCGTCGCCGTGGCGGACGAACCGCTCGCCGAGATCCGTGATCCCGCCGGCCAACTTAC
CGATGAGACCGGCAGCCGCCGCGGGCACGTCACCGGCACGTTCTTCCATCTGATCGATGTCGCCGAGGAGGCGATCGCCT
GA

Upstream 100 bases:

>100_bases
ATCCTGGTCGGCCCGTCACTCGCCGCCACCGACTTCCGCGAGAGCTCGCTCTACGCACCCGACTACGATCGCCGTTATCG
CCGCTCGGCGGAGGACGGCC

Downstream 100 bases:

>100_bases
TGTCCAAGTTGATTGAAAGCGCTCCGGCAGCCGGCCGCATCACGCTGGTCGGCGCCGGCCCGGGTGATGCCGACCTGCTG
ACGCTGCGCGCGATCAAGCG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MIVTDHILPTAPNGRPPGLIIAAPSTNSGKTTVTLALHAALRRRSLTVQPYKCGPDYIDPAFHEIAAGRPCYNLDSWAFG
QDRITSLLDAAAGADLCLAEGVMGLFDGGSARGAWGNGATADIAAFTGWPVVLVIDVAGQAQSAAALALGFARYRSDVRI
AGVILNNVASTRHEALVRDGFAAQDIPVLGGFERQRDLTLKNRHLGLVQARETARLDVRLQQLADSAVRGIDLDALIRAA
QPARPIASTIRLLTPPGQRIALAHDDAFSFIYPHLTAGWRAAGAEIVPFSPLNDEGPDPSCDAAWLPGGYPELHAGRLAA
ATRFKAALGAFAETRPVHGECGGYMTLGAGLIDADGTRHAMAGLLGLETDFASRKLHLGYRLATLHADAAGKAAGSVLRG
HEYHYARVVAVADEPLAEIRDPAGQLTDETGSRRGHVTGTFFHLIDVAEEAIA

Sequences:

>Translated_453_residues
MIVTDHILPTAPNGRPPGLIIAAPSTNSGKTTVTLALHAALRRRSLTVQPYKCGPDYIDPAFHEIAAGRPCYNLDSWAFG
QDRITSLLDAAAGADLCLAEGVMGLFDGGSARGAWGNGATADIAAFTGWPVVLVIDVAGQAQSAAALALGFARYRSDVRI
AGVILNNVASTRHEALVRDGFAAQDIPVLGGFERQRDLTLKNRHLGLVQARETARLDVRLQQLADSAVRGIDLDALIRAA
QPARPIASTIRLLTPPGQRIALAHDDAFSFIYPHLTAGWRAAGAEIVPFSPLNDEGPDPSCDAAWLPGGYPELHAGRLAA
ATRFKAALGAFAETRPVHGECGGYMTLGAGLIDADGTRHAMAGLLGLETDFASRKLHLGYRLATLHADAAGKAAGSVLRG
HEYHYARVVAVADEPLAEIRDPAGQLTDETGSRRGHVTGTFFHLIDVAEEAIA
>Mature_453_residues
MIVTDHILPTAPNGRPPGLIIAAPSTNSGKTTVTLALHAALRRRSLTVQPYKCGPDYIDPAFHEIAAGRPCYNLDSWAFG
QDRITSLLDAAAGADLCLAEGVMGLFDGGSARGAWGNGATADIAAFTGWPVVLVIDVAGQAQSAAALALGFARYRSDVRI
AGVILNNVASTRHEALVRDGFAAQDIPVLGGFERQRDLTLKNRHLGLVQARETARLDVRLQQLADSAVRGIDLDALIRAA
QPARPIASTIRLLTPPGQRIALAHDDAFSFIYPHLTAGWRAAGAEIVPFSPLNDEGPDPSCDAAWLPGGYPELHAGRLAA
ATRFKAALGAFAETRPVHGECGGYMTLGAGLIDADGTRHAMAGLLGLETDFASRKLHLGYRLATLHADAAGKAAGSVLRG
HEYHYARVVAVADEPLAEIRDPAGQLTDETGSRRGHVTGTFFHLIDVAEEAIA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 47762; Mature: 47762

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTDHILPTAPNGRPPGLIIAAPSTNSGKTTVTLALHAALRRRSLTVQPYKCGPDYIDP
CEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHCCCEECCCCCCCCCCCH
AFHEIAAGRPCYNLDSWAFGQDRITSLLDAAAGADLCLAEGVMGLFDGGSARGAWGNGAT
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHHCCCCCCCCCCCCCCCC
ADIAAFTGWPVVLVIDVAGQAQSAAALALGFARYRSDVRIAGVILNNVASTRHEALVRDG
CEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCC
FAAQDIPVLGGFERQRDLTLKNRHLGLVQARETARLDVRLQQLADSAVRGIDLDALIRAA
CCCCCCCCCCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHC
QPARPIASTIRLLTPPGQRIALAHDDAFSFIYPHLTAGWRAAGAEIVPFSPLNDEGPDPS
CCCCHHHHHHEEECCCCCEEEEECCCCHHHHHHHHCCCCHHCCCEEEECCCCCCCCCCCC
CDAAWLPGGYPELHAGRLAAATRFKAALGAFAETRPVHGECGGYMTLGAGLIDADGTRHA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCCCHHH
MAGLLGLETDFASRKLHLGYRLATLHADAAGKAAGSVLRGHEYHYARVVAVADEPLAEIR
HHHHHHCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHCCCCEEEEEEEEECCCCHHHHC
DPAGQLTDETGSRRGHVTGTFFHLIDVAEEAIA
CCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIVTDHILPTAPNGRPPGLIIAAPSTNSGKTTVTLALHAALRRRSLTVQPYKCGPDYIDP
CEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHCCCEECCCCCCCCCCCH
AFHEIAAGRPCYNLDSWAFGQDRITSLLDAAAGADLCLAEGVMGLFDGGSARGAWGNGAT
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHHCCCCCCCCCCCCCCCC
ADIAAFTGWPVVLVIDVAGQAQSAAALALGFARYRSDVRIAGVILNNVASTRHEALVRDG
CEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCC
FAAQDIPVLGGFERQRDLTLKNRHLGLVQARETARLDVRLQQLADSAVRGIDLDALIRAA
CCCCCCCCCCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHC
QPARPIASTIRLLTPPGQRIALAHDDAFSFIYPHLTAGWRAAGAEIVPFSPLNDEGPDPS
CCCCHHHHHHEEECCCCCEEEEECCCCHHHHHHHHCCCCHHCCCEEEECCCCCCCCCCCC
CDAAWLPGGYPELHAGRLAAATRFKAALGAFAETRPVHGECGGYMTLGAGLIDADGTRHA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCCCHHH
MAGLLGLETDFASRKLHLGYRLATLHADAAGKAAGSVLRGHEYHYARVVAVADEPLAEIR
HHHHHHCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHCCCCEEEEEEEEECCCCHHHHC
DPAGQLTDETGSRRGHVTGTFFHLIDVAEEAIA
CCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]