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The map label for this gene is gph [H]

Identifier: 192290584

GI number: 192290584

Start: 2376296

End: 2376973

Strand: Reverse

Name: gph [H]

Synonym: Rpal_2193

Alternate gene names: 192290584

Gene position: 2376973-2376296 (Counterclockwise)

Preceding gene: 192290591

Following gene: 192290583

Centisome position: 41.38

GC content: 66.52

Gene sequence:

>678_bases
ATGACCGCATCTCCCATTGTTGTCTTCGATCTCGACGGCACGCTGATCGACACCGCGCCCGACCTGATCAACGCCCTGAA
TTTCATCCTCGTTCGCGAAGGCATGCCGGCCGTTCCGATGGCCGTCGCGCGCAACATGATCGGACAAGGCGCTCGCCGCC
TGCTCGAACGTGGCCTCGAACTCGACGGCCGTGTGATCGCGCAGGACGACGTCAATCGCCTGACGGTCGATTTCATCGAC
TATTACGCCGCCCACATCGCCGACGAGTCGCGCCCGTTCGAAGGGCTGGAAGCGACGCTCGACGAGCTGTCGGAGAGCGG
CTACCGGTTTGCGGTCTGCACCAACAAGCTCGAATGGCTGTCGAAGCTGCTGCTCGACCGGCTCGGGCTCAGCCCGCGCT
TTGCGGCGATCTGCGGCGCGGACACTTTCGGTGTTGCCAAGCCTGACCCGGCGATCCTGCGCGAGACCGTGGCGAAGGCC
GGCGGCGACCTGTCGGCGGCCATCATGGTCGGCGACGCGGGACCGGATGTCGGTGTCGCACGCCGGGCCGGCGTGCCGGT
GATCGGCGTCGAATTCGGCTACACCGAAGTGCCGATCGCCGAGCTCCAGCCGGACCTGTTGGTCGGCCATATGCGCGAGT
TGCCGCATGCGGTCGGTCGGCTGCTGCCGCGACGCTGA

Upstream 100 bases:

>100_bases
GCATATCTAGCGGCGTTTGGGCGCGGCGAACAGCACGGATTCTGGGGTCGAGCATCGCCAAACGGTTGCAGAAACCCGCA
GAGAAGGTTAGCGGTCGAAC

Downstream 100 bases:

>100_bases
CCCGAAGGCGATACCTTTGAAATTGTGGCGAAATTCAGTTCGCTAACAGGTCGCGTTAACTAACTCTTAAGTTTGTTGGA
CGCGCAGTTGCCAGCGTTCC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTASPIVVFDLDGTLIDTAPDLINALNFILVREGMPAVPMAVARNMIGQGARRLLERGLELDGRVIAQDDVNRLTVDFID
YYAAHIADESRPFEGLEATLDELSESGYRFAVCTNKLEWLSKLLLDRLGLSPRFAAICGADTFGVAKPDPAILRETVAKA
GGDLSAAIMVGDAGPDVGVARRAGVPVIGVEFGYTEVPIAELQPDLLVGHMRELPHAVGRLLPRR

Sequences:

>Translated_225_residues
MTASPIVVFDLDGTLIDTAPDLINALNFILVREGMPAVPMAVARNMIGQGARRLLERGLELDGRVIAQDDVNRLTVDFID
YYAAHIADESRPFEGLEATLDELSESGYRFAVCTNKLEWLSKLLLDRLGLSPRFAAICGADTFGVAKPDPAILRETVAKA
GGDLSAAIMVGDAGPDVGVARRAGVPVIGVEFGYTEVPIAELQPDLLVGHMRELPHAVGRLLPRR
>Mature_224_residues
TASPIVVFDLDGTLIDTAPDLINALNFILVREGMPAVPMAVARNMIGQGARRLLERGLELDGRVIAQDDVNRLTVDFIDY
YAAHIADESRPFEGLEATLDELSESGYRFAVCTNKLEWLSKLLLDRLGLSPRFAAICGADTFGVAKPDPAILRETVAKAG
GDLSAAIMVGDAGPDVGVARRAGVPVIGVEFGYTEVPIAELQPDLLVGHMRELPHAVGRLLPRR

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=232, Percent_Identity=29.3103448275862, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24216; Mature: 24085

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPIVVFDLDGTLIDTAPDLINALNFILVREGMPAVPMAVARNMIGQGARRLLERGLE
CCCCCEEEEECCCCEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
LDGRVIAQDDVNRLTVDFIDYYAAHIADESRPFEGLEATLDELSESGYRFAVCTNKLEWL
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHH
SKLLLDRLGLSPRFAAICGADTFGVAKPDPAILRETVAKAGGDLSAAIMVGDAGPDVGVA
HHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHH
RRAGVPVIGVEFGYTEVPIAELQPDLLVGHMRELPHAVGRLLPRR
HHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TASPIVVFDLDGTLIDTAPDLINALNFILVREGMPAVPMAVARNMIGQGARRLLERGLE
CCCCEEEEECCCCEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
LDGRVIAQDDVNRLTVDFIDYYAAHIADESRPFEGLEATLDELSESGYRFAVCTNKLEWL
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHH
SKLLLDRLGLSPRFAAICGADTFGVAKPDPAILRETVAKAGGDLSAAIMVGDAGPDVGVA
HHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHH
RRAGVPVIGVEFGYTEVPIAELQPDLLVGHMRELPHAVGRLLPRR
HHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]