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The map label for this gene is fieF [H]

Identifier: 192290540

GI number: 192290540

Start: 2326729

End: 2328111

Strand: Reverse

Name: fieF [H]

Synonym: Rpal_2149

Alternate gene names: 192290540

Gene position: 2328111-2326729 (Counterclockwise)

Preceding gene: 192290541

Following gene: 192290539

Centisome position: 40.53

GC content: 67.1

Gene sequence:

>1383_bases
ATGAACGACGTCGCCAGCACCAAGTCGCGAGTGGCCGCGGTCTCCGTGGTGGCGAGTGCCACCATGGCCGCGGCGAAATT
CGCCGTCGGCATCGCCATCGGTTCGCTGGCGCTGATCTCCGAAGCGCTTCACAGCTCGATCGATCTGGTCGCCACCATCA
TCACCTGGTTCGTGGTGCGGATCTCCGATCGCCCCGCCGACGACGCGCATCACTACGGTCACGGCAAGATCGAGAGCCTG
TCGGCGCTCGGCGTGATCGCGATGCTGTATGTGCTGGCCGGCGGCATCCTGGTCGAAGCCTACAGCCGGCTGCGCGAGGG
CGCGGCGCCGCCGACCTTCTCGTATCTGGCCTTCGTCATCCTCGGCGTCGATATCGTCGTCAATCTGTGGCGCGCCTGGG
CCCTGCACCGCACCGCGGTCGCCACCAAGAGCCAGGCGCTCGCCGCCGACGCGCTGCATTTCGCCTCCGACGTGCTCGGC
TCGTTCGCGGTGATCGCTGGCCTCGCGCTCGCCGCCGCTGGATTCCATTGGGGTGACGCCGCGGCCGCGATCGGCGTCGC
GGTGATGATCTCGCTGCTCGGGCTGCGGCTCGGCCGCTCCACGATTCAGACCCTGCTCGATCGCGCCCCCGAAGGCGTGT
CGGAGAAGGCGCGCGAAGCGATCGGCGCCGTACCGGGTGTGGTCGGCGTCGAGCGGCTGCGCGCCCGCATGGTCGGACCG
ACGCACTTCATCGACGCCCTGGTCGAAGTGCCCCGCACCTATCCGATCGACCGCATCGACGAGATCAAGCGCAAGGCGCA
GGAATCGATGTCCGAGATCTTCGGCGACGCCGACCTCACCTTCACGGCGATTCCGGTGGCGCGCGACAATGAGAGCGTGC
GCGAGCGCATCATGGTGATCGCGCGCAATTCCGGCCTCGCCGTCCACCATGTCACCGTCCACGATCTCGGCGGCCACAAG
CTGATCGTCAGCATCGATCTCGAAGTCGACGGCAACATGCCGCTGATCGAAGCGCACGATATCGCACACAAGCTCGAGGA
TCACATCCGCGAGGACTTCGGTGCCGACGTCGAAGTCGACACCCACATCGAGCCGCTGGAGCCGGAGCTGCCGCAGGGCT
CGGACGCCGCGCCCGCGCGCGTCGAAGAGATTCGGCTGGCGCTACGCCGGCTCACCCACGATGGCGCGATCCACGATGTG
CACAGCGTCCGCGTGCGCGACACCGATGCCGGCGAGATCGTCAACTTCCACTGCTACGCCGATCCGTCGATGAGCGTGAT
CTCGGTGCACGACCACGTCGACGAAATCGAGCGCAAGCTGCGCGCCAAGTTCCCCTCGGTAAAGCGCGTGATCAGTCACG
CCGAACCGCCGAACGCCGACTGA

Upstream 100 bases:

>100_bases
ACTGAGACTAATTCGCAGCTACTGCAAACCGGCCCGTTACCACCAGGGTAACGGGTCGCGCGCCTTTCCGGCGTCGCACG
AGGCTGCTAAATCCCCCGCC

Downstream 100 bases:

>100_bases
GCTGAAAGCGCCGCAACAAACTTTGCGGCCGTTCTCCTTTCGGACTCGACTCACGAAAAAATGTCGGCGCGATTCACCTT
TGGATAAGAATTTTTCCGAC

Product: cation diffusion facilitator family transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNDVASTKSRVAAVSVVASATMAAAKFAVGIAIGSLALISEALHSSIDLVATIITWFVVRISDRPADDAHHYGHGKIESL
SALGVIAMLYVLAGGILVEAYSRLREGAAPPTFSYLAFVILGVDIVVNLWRAWALHRTAVATKSQALAADALHFASDVLG
SFAVIAGLALAAAGFHWGDAAAAIGVAVMISLLGLRLGRSTIQTLLDRAPEGVSEKAREAIGAVPGVVGVERLRARMVGP
THFIDALVEVPRTYPIDRIDEIKRKAQESMSEIFGDADLTFTAIPVARDNESVRERIMVIARNSGLAVHHVTVHDLGGHK
LIVSIDLEVDGNMPLIEAHDIAHKLEDHIREDFGADVEVDTHIEPLEPELPQGSDAAPARVEEIRLALRRLTHDGAIHDV
HSVRVRDTDAGEIVNFHCYADPSMSVISVHDHVDEIERKLRAKFPSVKRVISHAEPPNAD

Sequences:

>Translated_460_residues
MNDVASTKSRVAAVSVVASATMAAAKFAVGIAIGSLALISEALHSSIDLVATIITWFVVRISDRPADDAHHYGHGKIESL
SALGVIAMLYVLAGGILVEAYSRLREGAAPPTFSYLAFVILGVDIVVNLWRAWALHRTAVATKSQALAADALHFASDVLG
SFAVIAGLALAAAGFHWGDAAAAIGVAVMISLLGLRLGRSTIQTLLDRAPEGVSEKAREAIGAVPGVVGVERLRARMVGP
THFIDALVEVPRTYPIDRIDEIKRKAQESMSEIFGDADLTFTAIPVARDNESVRERIMVIARNSGLAVHHVTVHDLGGHK
LIVSIDLEVDGNMPLIEAHDIAHKLEDHIREDFGADVEVDTHIEPLEPELPQGSDAAPARVEEIRLALRRLTHDGAIHDV
HSVRVRDTDAGEIVNFHCYADPSMSVISVHDHVDEIERKLRAKFPSVKRVISHAEPPNAD
>Mature_460_residues
MNDVASTKSRVAAVSVVASATMAAAKFAVGIAIGSLALISEALHSSIDLVATIITWFVVRISDRPADDAHHYGHGKIESL
SALGVIAMLYVLAGGILVEAYSRLREGAAPPTFSYLAFVILGVDIVVNLWRAWALHRTAVATKSQALAADALHFASDVLG
SFAVIAGLALAAAGFHWGDAAAAIGVAVMISLLGLRLGRSTIQTLLDRAPEGVSEKAREAIGAVPGVVGVERLRARMVGP
THFIDALVEVPRTYPIDRIDEIKRKAQESMSEIFGDADLTFTAIPVARDNESVRERIMVIARNSGLAVHHVTVHDLGGHK
LIVSIDLEVDGNMPLIEAHDIAHKLEDHIREDFGADVEVDTHIEPLEPELPQGSDAAPARVEEIRLALRRLTHDGAIHDV
HSVRVRDTDAGEIVNFHCYADPSMSVISVHDHVDEIERKLRAKFPSVKRVISHAEPPNAD

Specific function: Cation-efflux transporter that may have a role in detoxification [H]

COG id: COG0053

COG function: function code P; Predicted Co/Zn/Cd cation transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. FieF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790349, Length=261, Percent_Identity=30.6513409961686, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002524 [H]

Pfam domain/function: PF01545 Cation_efflux [H]

EC number: NA

Molecular weight: Translated: 49409; Mature: 49409

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVASTKSRVAAVSVVASATMAAAKFAVGIAIGSLALISEALHSSIDLVATIITWFVVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISDRPADDAHHYGHGKIESLSALGVIAMLYVLAGGILVEAYSRLREGAAPPTFSYLAFVI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LGVDIVVNLWRAWALHRTAVATKSQALAADALHFASDVLGSFAVIAGLALAAAGFHWGDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AAAIGVAVMISLLGLRLGRSTIQTLLDRAPEGVSEKAREAIGAVPGVVGVERLRARMVGP
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCH
THFIDALVEVPRTYPIDRIDEIKRKAQESMSEIFGDADLTFTAIPVARDNESVRERIMVI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHEEEE
ARNSGLAVHHVTVHDLGGHKLIVSIDLEVDGNMPLIEAHDIAHKLEDHIREDFGADVEVD
ECCCCEEEEEEEEEECCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCEEC
THIEPLEPELPQGSDAAPARVEEIRLALRRLTHDGAIHDVHSVRVRDTDAGEIVNFHCYA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCCEEEEEEEC
DPSMSVISVHDHVDEIERKLRAKFPSVKRVISHAEPPNAD
CCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNDVASTKSRVAAVSVVASATMAAAKFAVGIAIGSLALISEALHSSIDLVATIITWFVVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISDRPADDAHHYGHGKIESLSALGVIAMLYVLAGGILVEAYSRLREGAAPPTFSYLAFVI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LGVDIVVNLWRAWALHRTAVATKSQALAADALHFASDVLGSFAVIAGLALAAAGFHWGDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AAAIGVAVMISLLGLRLGRSTIQTLLDRAPEGVSEKAREAIGAVPGVVGVERLRARMVGP
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCH
THFIDALVEVPRTYPIDRIDEIKRKAQESMSEIFGDADLTFTAIPVARDNESVRERIMVI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHEEEE
ARNSGLAVHHVTVHDLGGHKLIVSIDLEVDGNMPLIEAHDIAHKLEDHIREDFGADVEVD
ECCCCEEEEEEEEEECCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCEEC
THIEPLEPELPQGSDAAPARVEEIRLALRRLTHDGAIHDVHSVRVRDTDAGEIVNFHCYA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCCEEEEEEEC
DPSMSVISVHDHVDEIERKLRAKFPSVKRVISHAEPPNAD
CCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA