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The map label for this gene is amiF [H]

Identifier: 192290526

GI number: 192290526

Start: 2303657

End: 2304679

Strand: Reverse

Name: amiF [H]

Synonym: Rpal_2134

Alternate gene names: 192290526

Gene position: 2304679-2303657 (Counterclockwise)

Preceding gene: 192290527

Following gene: 192290510

Centisome position: 40.12

GC content: 62.27

Gene sequence:

>1023_bases
ATGGCAAGTACCGGAAGCGTAAGCGCGTGGGACGAAGCCCTGCTCGTTGCGATGATCCAATATCCCGTGCCGGTGGTCAG
CGGGCCGGAGGATATCCAAACCCAGGTCAAGCAGATCTGCCGCGCGGTCGACACCACCAAAGCCGGCTACCCCGATCTCG
ATCTGATCGTGTTCCCCGAATATTCGACTCAGGGTCTCAACACCGCGATCTGGACCTACGACGAGATGCTGCTGACGGTG
GATTCGCCGGAGATCGGCGCGTTCAAGCAGGCGTGCAAGCGCAACAAGGTCTGGGGCGTGTTCTCGCTGATGGAGCGCAA
CGACGATCCGTCGCTGCCGCCGTTCAACACCGCGATCATCATCAACGCCGACGGCGAACTGGCGCTGCATTATCGCAAGC
TGCAGCCGTGGGTGCCGATCGAGCCGTGGTCGCCGGGTAATTACGGCATGCCGGTGTGCGACGGCCCGAAGGGCTCGAAA
CTCGCGGTCTGCATCTGCCACGACGGCATGTTCCCGGAGCTGGCGCGCGAGGCCGCCTACAAGGGCGCCAACGTCTACAT
CCGGATCTCCGGCTATTCGACCCAGGTCAATGATCAGTGGATCTGGACCAACCGGACCAACGCCTGGCAGAACCTGATGT
ACACCATGTCGGTCAATCTGGCCGGCTACGACGGCGTGTTCTATTACTTCGGCGAAGGCACGGTGTGCAATTACGACGGC
AACGTCATCCAGCAGGGCCACCGCAATCCCTGGGAGATCGTCACCGCCGAACTGTTCCCCCGCCTCGTCGACAAGGCGCG
CGAGAACTGGGCGCTGGAGAACAACATCTTCAACCTCGGCTGCCGCGCCTATGTCGGCAAGCCCGGCGGCGAAAAGGCGA
ACTACCTGACCTGGGTGCGCGATCTCGCCGAAGGCAACTACAAGCTTCCCTGGGACGACAAGATCAAGGTCCGCGACGGC
TGGAAGTATTATCCGGACGGCGTCAAGCTCGGCCCGATGCCGAAGCCGATCGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
CTGCTCAAGATTACGAAGCCCGTTACGTAGAACAGCGTATTCGCCACCGGCCGCTGCAGTTCCATAGACGTGACGTTCTA
ACGTTACATGGAGCCAAGTT

Downstream 100 bases:

>100_bases
CTCCCACCGATCTCGTCATCCCAAGGGAGACCCGGCCGCCACGGCGACCGGGTCCTTGAGTGAGGAGCGGGCTTGAATGC
CCGAGCGCCCTCGAACACTT

Product: formamidase

Products: NA

Alternate protein names: Formamide amidohydrolase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MASTGSVSAWDEALLVAMIQYPVPVVSGPEDIQTQVKQICRAVDTTKAGYPDLDLIVFPEYSTQGLNTAIWTYDEMLLTV
DSPEIGAFKQACKRNKVWGVFSLMERNDDPSLPPFNTAIIINADGELALHYRKLQPWVPIEPWSPGNYGMPVCDGPKGSK
LAVCICHDGMFPELAREAAYKGANVYIRISGYSTQVNDQWIWTNRTNAWQNLMYTMSVNLAGYDGVFYYFGEGTVCNYDG
NVIQQGHRNPWEIVTAELFPRLVDKARENWALENNIFNLGCRAYVGKPGGEKANYLTWVRDLAEGNYKLPWDDKIKVRDG
WKYYPDGVKLGPMPKPIAAE

Sequences:

>Translated_340_residues
MASTGSVSAWDEALLVAMIQYPVPVVSGPEDIQTQVKQICRAVDTTKAGYPDLDLIVFPEYSTQGLNTAIWTYDEMLLTV
DSPEIGAFKQACKRNKVWGVFSLMERNDDPSLPPFNTAIIINADGELALHYRKLQPWVPIEPWSPGNYGMPVCDGPKGSK
LAVCICHDGMFPELAREAAYKGANVYIRISGYSTQVNDQWIWTNRTNAWQNLMYTMSVNLAGYDGVFYYFGEGTVCNYDG
NVIQQGHRNPWEIVTAELFPRLVDKARENWALENNIFNLGCRAYVGKPGGEKANYLTWVRDLAEGNYKLPWDDKIKVRDG
WKYYPDGVKLGPMPKPIAAE
>Mature_339_residues
ASTGSVSAWDEALLVAMIQYPVPVVSGPEDIQTQVKQICRAVDTTKAGYPDLDLIVFPEYSTQGLNTAIWTYDEMLLTVD
SPEIGAFKQACKRNKVWGVFSLMERNDDPSLPPFNTAIIINADGELALHYRKLQPWVPIEPWSPGNYGMPVCDGPKGSKL
AVCICHDGMFPELAREAAYKGANVYIRISGYSTQVNDQWIWTNRTNAWQNLMYTMSVNLAGYDGVFYYFGEGTVCNYDGN
VIQQGHRNPWEIVTAELFPRLVDKARENWALENNIFNLGCRAYVGKPGGEKANYLTWVRDLAEGNYKLPWDDKIKVRDGW
KYYPDGVKLGPMPKPIAAE

Specific function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323383, Length=282, Percent_Identity=25.531914893617, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022843
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.49 [H]

Molecular weight: Translated: 38231; Mature: 38100

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTGSVSAWDEALLVAMIQYPVPVVSGPEDIQTQVKQICRAVDTTKAGYPDLDLIVFPE
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
YSTQGLNTAIWTYDEMLLTVDSPEIGAFKQACKRNKVWGVFSLMERNDDPSLPPFNTAII
CCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCEEEE
INADGELALHYRKLQPWVPIEPWSPGNYGMPVCDGPKGSKLAVCICHDGMFPELAREAAY
EECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHC
KGANVYIRISGYSTQVNDQWIWTNRTNAWQNLMYTMSVNLAGYDGVFYYFGEGTVCNYDG
CCCEEEEEEECCEEECCCEEEEECCCCHHHEEEEEEEEEEECCCCEEEEECCCEEEECCC
NVIQQGHRNPWEIVTAELFPRLVDKARENWALENNIFNLGCRAYVGKPGGEKANYLTWVR
CHHHCCCCCHHHEEEHHHHHHHHHHHHHCCEECCCEEEECEEEEECCCCCCCCHHHHHHH
DLAEGNYKLPWDDKIKVRDGWKYYPDGVKLGPMPKPIAAE
HHHCCCEECCCCCEEEEECCCEECCCCEEECCCCCCCCCC
>Mature Secondary Structure 
ASTGSVSAWDEALLVAMIQYPVPVVSGPEDIQTQVKQICRAVDTTKAGYPDLDLIVFPE
CCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
YSTQGLNTAIWTYDEMLLTVDSPEIGAFKQACKRNKVWGVFSLMERNDDPSLPPFNTAII
CCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCEEEE
INADGELALHYRKLQPWVPIEPWSPGNYGMPVCDGPKGSKLAVCICHDGMFPELAREAAY
EECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHC
KGANVYIRISGYSTQVNDQWIWTNRTNAWQNLMYTMSVNLAGYDGVFYYFGEGTVCNYDG
CCCEEEEEEECCEEECCCEEEEECCCCHHHEEEEEEEEEEECCCCEEEEECCCEEEECCC
NVIQQGHRNPWEIVTAELFPRLVDKARENWALENNIFNLGCRAYVGKPGGEKANYLTWVR
CHHHCCCCCHHHEEEHHHHHHHHHHHHHCCEECCCEEEECEEEEECCCCCCCCHHHHHHH
DLAEGNYKLPWDDKIKVRDGWKYYPDGVKLGPMPKPIAAE
HHHCCCEECCCCCEEEEECCCEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]