Click here to switch to the map view.

The map label for this gene is actII-2 [H]

Identifier: 192290527

GI number: 192290527

Start: 2305533

End: 2306999

Strand: Reverse

Name: actII-2 [H]

Synonym: Rpal_2136

Alternate gene names: 192290527

Gene position: 2306999-2305533 (Counterclockwise)

Preceding gene: 192290529

Following gene: 192290526

Centisome position: 40.16

GC content: 63.53

Gene sequence:

>1467_bases
ATGACCGTCGATCACCCACCTGCGACATCGCATGTCCGGCAGACCGCCGCCAAAACACGCCCCTCGGCGCTGGCTCTGAC
CATCCTGTTGCTGGGCGGGTTCATCACCGTCTTCGACCTGTTTGTGGTCAACATTGCGATCCCCTCGATGCAGGCCGATC
TTGGTGTGAGCTTTGCCGAAATCGGCTTCGTCATCGCCGGATATGAACTGGCCTTCGGCGTGCTGCTGATCGCGGGCGGG
CGGCTCGGCGACCGCCATGGCAGACGTCGCCTGTTCGCACTCGGCATGGCCGGATTTGCGATCACATCGTTCCTCTGCGG
CATCGCGCCCGACGCCACCAGCCTGATCGTTGCCCGCTTTCTGCAAGGCGCGACAGGCGCGATCCTGTTTCCGCAGGTCT
ATGCGCTGCTACGTGTCATGTACGACGATGCTGGCCGCCGCCGGGCATTCGGACTTCTCGGCATGACATTGGGGCTGGCG
GCCATCGTCGGACAGGTCTTCGGCGGCCTGATCGTTGAAAGCGACCTGTTCGACCTCGGCTGGCGTATCATCTTCCTCAT
CAATCTACCGATAGGCATCATCGCGCTGCTATCGGCGCGGATCATCCCGGAATCGCGCTCTTCCGACGCGATGGGCGTCG
ATTGGCCCGGGATCGGACTGGCGACAGCGGGATTGCTGCTCCTGCTGTTGCCTCTGCTCGAAGGCCCGAATCTCGGCTGG
CCGCTCTGGACATGGGCCAGCATGGCAGCCTCGGTCGTGCTGCTCACGGCGTTTCTCGTCTGGGAGCATCTGGTTGCAAG
GCGCGGCGGCGATCCCGCCATTGATCTGACCTTGTTCCGCAACACCGGCTTCTCGGTGGGGTCTGTGGTAGTGCTGCTGA
TCTATTCGACAGCGACATCGCTGTTCCTATGCTTCGCCCTGCTGGTGCAGTCCGGCCTCGGTCTGACGCCGTTCGCGGCC
GGCACGCTGTTCGCGCCGGCGAGCGTCGGCTTCGTGGTGGCATCGCTGGTCGCACCGAAGCTGGTGGCGCGTTTCGGAAA
CATCGCAATCGCCGGTGGCACGCTGGTCTATGCAGCCGGCATCGGCTGGCTGATCGTGACCGCCGCAGGTCTCGGCGACG
GAGCTGGAATATTCGCGCTGCTGCCGCCGCTGGTGCTGTTCGGCTTCGGACAGGGCCTGTCGATGACGCCGCTGCTCAAT
CTGGTCATCGGCTATGTCGAGGAAAGTCATGCCGGAATGGCGGCCGGCTTTATTTCCACCATGCAACAGGTCGGCGGAGC
CTTCGGCGTTTCCGTTGTTGGCATATTCTTCGTGGGGATTCTCGGTAAGGATGCCGGCGAAGGCGTCTCCCAGGCAGGGC
GCTACGCGGACGCCTTCTCCGGCGCAATGATCTACAATCTCGCCGCCGTCATCGTCGCTGCCGCGCTCATTGCCTTGATC
GTGCGCAAGAGCCCCGCCCGTCAGTAG

Upstream 100 bases:

>100_bases
AGCCGATGATCCAGCGTATCACTCATAGTAATAGTATAATTTGCTCCATAGTAATAGATTAATGCGCGTGCGAAGCGACT
TCAAGCACATTGGAGTTGCC

Downstream 100 bases:

>100_bases
AGGGCGAGGAATCTGCGTCGGCGGTCTTCAAGGCAACCGCCGAACAAGATGCTCGTCCCCGCCCCGCGCCGTGCTTGACA
CCTCGCCGGCCCGCACATCG

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTVDHPPATSHVRQTAAKTRPSALALTILLLGGFITVFDLFVVNIAIPSMQADLGVSFAEIGFVIAGYELAFGVLLIAGG
RLGDRHGRRRLFALGMAGFAITSFLCGIAPDATSLIVARFLQGATGAILFPQVYALLRVMYDDAGRRRAFGLLGMTLGLA
AIVGQVFGGLIVESDLFDLGWRIIFLINLPIGIIALLSARIIPESRSSDAMGVDWPGIGLATAGLLLLLLPLLEGPNLGW
PLWTWASMAASVVLLTAFLVWEHLVARRGGDPAIDLTLFRNTGFSVGSVVVLLIYSTATSLFLCFALLVQSGLGLTPFAA
GTLFAPASVGFVVASLVAPKLVARFGNIAIAGGTLVYAAGIGWLIVTAAGLGDGAGIFALLPPLVLFGFGQGLSMTPLLN
LVIGYVEESHAGMAAGFISTMQQVGGAFGVSVVGIFFVGILGKDAGEGVSQAGRYADAFSGAMIYNLAAVIVAAALIALI
VRKSPARQ

Sequences:

>Translated_488_residues
MTVDHPPATSHVRQTAAKTRPSALALTILLLGGFITVFDLFVVNIAIPSMQADLGVSFAEIGFVIAGYELAFGVLLIAGG
RLGDRHGRRRLFALGMAGFAITSFLCGIAPDATSLIVARFLQGATGAILFPQVYALLRVMYDDAGRRRAFGLLGMTLGLA
AIVGQVFGGLIVESDLFDLGWRIIFLINLPIGIIALLSARIIPESRSSDAMGVDWPGIGLATAGLLLLLLPLLEGPNLGW
PLWTWASMAASVVLLTAFLVWEHLVARRGGDPAIDLTLFRNTGFSVGSVVVLLIYSTATSLFLCFALLVQSGLGLTPFAA
GTLFAPASVGFVVASLVAPKLVARFGNIAIAGGTLVYAAGIGWLIVTAAGLGDGAGIFALLPPLVLFGFGQGLSMTPLLN
LVIGYVEESHAGMAAGFISTMQQVGGAFGVSVVGIFFVGILGKDAGEGVSQAGRYADAFSGAMIYNLAAVIVAAALIALI
VRKSPARQ
>Mature_487_residues
TVDHPPATSHVRQTAAKTRPSALALTILLLGGFITVFDLFVVNIAIPSMQADLGVSFAEIGFVIAGYELAFGVLLIAGGR
LGDRHGRRRLFALGMAGFAITSFLCGIAPDATSLIVARFLQGATGAILFPQVYALLRVMYDDAGRRRAFGLLGMTLGLAA
IVGQVFGGLIVESDLFDLGWRIIFLINLPIGIIALLSARIIPESRSSDAMGVDWPGIGLATAGLLLLLLPLLEGPNLGWP
LWTWASMAASVVLLTAFLVWEHLVARRGGDPAIDLTLFRNTGFSVGSVVVLLIYSTATSLFLCFALLVQSGLGLTPFAAG
TLFAPASVGFVVASLVAPKLVARFGNIAIAGGTLVYAAGIGWLIVTAAGLGDGAGIFALLPPLVLFGFGQGLSMTPLLNL
VIGYVEESHAGMAAGFISTMQQVGGAFGVSVVGIFFVGILGKDAGEGVSQAGRYADAFSGAMIYNLAAVIVAAALIALIV
RKSPARQ

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=443, Percent_Identity=27.765237020316, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789042, Length=418, Percent_Identity=27.0334928229665, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=27.7227722772277, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081983, Length=417, Percent_Identity=26.378896882494, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788392, Length=462, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790146, Length=179, Percent_Identity=27.9329608938547, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50590; Mature: 50458

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDHPPATSHVRQTAAKTRPSALALTILLLGGFITVFDLFVVNIAIPSMQADLGVSFAE
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHH
IGFVIAGYELAFGVLLIAGGRLGDRHGRRRLFALGMAGFAITSFLCGIAPDATSLIVARF
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LQGATGAILFPQVYALLRVMYDDAGRRRAFGLLGMTLGLAAIVGQVFGGLIVESDLFDLG
HCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
WRIIFLINLPIGIIALLSARIIPESRSSDAMGVDWPGIGLATAGLLLLLLPLLEGPNLGW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
PLWTWASMAASVVLLTAFLVWEHLVARRGGDPAIDLTLFRNTGFSVGSVVVLLIYSTATS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LFLCFALLVQSGLGLTPFAAGTLFAPASVGFVVASLVAPKLVARFGNIAIAGGTLVYAAG
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHH
IGWLIVTAAGLGDGAGIFALLPPLVLFGFGQGLSMTPLLNLVIGYVEESHAGMAAGFIST
HHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
MQQVGGAFGVSVVGIFFVGILGKDAGEGVSQAGRYADAFSGAMIYNLAAVIVAAALIALI
HHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VRKSPARQ
HHCCCCCC
>Mature Secondary Structure 
TVDHPPATSHVRQTAAKTRPSALALTILLLGGFITVFDLFVVNIAIPSMQADLGVSFAE
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHH
IGFVIAGYELAFGVLLIAGGRLGDRHGRRRLFALGMAGFAITSFLCGIAPDATSLIVARF
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LQGATGAILFPQVYALLRVMYDDAGRRRAFGLLGMTLGLAAIVGQVFGGLIVESDLFDLG
HCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
WRIIFLINLPIGIIALLSARIIPESRSSDAMGVDWPGIGLATAGLLLLLLPLLEGPNLGW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
PLWTWASMAASVVLLTAFLVWEHLVARRGGDPAIDLTLFRNTGFSVGSVVVLLIYSTATS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LFLCFALLVQSGLGLTPFAAGTLFAPASVGFVVASLVAPKLVARFGNIAIAGGTLVYAAG
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHH
IGWLIVTAAGLGDGAGIFALLPPLVLFGFGQGLSMTPLLNLVIGYVEESHAGMAAGFIST
HHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
MQQVGGAFGVSVVGIFFVGILGKDAGEGVSQAGRYADAFSGAMIYNLAAVIVAAALIALI
HHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VRKSPARQ
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]