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The map label for this gene is paaF [C]

Identifier: 192290305

GI number: 192290305

Start: 2061890

End: 2062672

Strand: Reverse

Name: paaF [C]

Synonym: Rpal_1912

Alternate gene names: 192290305

Gene position: 2062672-2061890 (Counterclockwise)

Preceding gene: 192290310

Following gene: 192290296

Centisome position: 35.91

GC content: 68.58

Gene sequence:

>783_bases
ATGTGCGACAGCGACACTGCCAGCGCGCCGGTGCTGACCATCGACGGTCCTCGCGCCACCATCCGACTGAACCGCCCCAA
GCACCTCAACCGGCTGCAGCCGGACGACCTCGCCACCCTGCTCGATCAATTCGCGCGGCTCGATGCCGATCCGGCGGTGC
GGGTGCTGGTGCTCACCGGCACCGGCCGGGCGTTCTCGGCGGGCTTCGATCTCGGCGAGGTCGCCGAACGCGCCACCGCG
GAGGTCGAGGCCGAATCCCCCGGGTCGGCGTTCGAAGTCGTGGTCAACCGGCTCGAGGATCTGCGGGTGCCGACGATCTG
CCGACTCAACGGCGGCGTCTATGGCGGCTCGACTGATCTGGCCCTGGCCTGTGATTTCCGCATCGGCGTCGACACCGCCG
AGATGTTCATGCCTGCCGCAAGGCTCGGGCTGCATTACTATCCGGGCGGCATCAAGCGCTACGTCTCCCGCCTCGGCATC
GACAACGCCAAGCGGCTGTTCCTCACCGCGCAGCGGATCGAAGCGGCCGAGATGCTGCGGATCGGCTATCTCACCGCGAT
GGTGCCGATGGACCGGCTCGACCGCGAGGTCGACGCCTTGGCGACGACGCTGGCCGGCAACGCGCCGAACGCGGTCGCCG
GCATGAAGCGCGCCATCAACGAATTCGCCCGCGCCGCGTTCGACGAACAAGCCGCCGACACCCGCGCCCGCGCCTCGATG
CGCAGCGACGAGATCAAGGAAGGCATCGCGGCGTTCACGGAAAAGCGTGCGCCGCGGTTTTGA

Upstream 100 bases:

>100_bases
GCCCGGCCTATTTTCCCATCCGCCGCGGCGTTGTCCGCGGTCTTTGCAGGGCCCGGCGCCGTTCCGGCAGCCGCCCGCTT
CCGATCAGGAGACCTGACCA

Downstream 100 bases:

>100_bases
AACTCATGCTTCGCAGACTCTCCGTCGTCATTCCGGGCGCGAAGCCGCGCAAATCCTATCCGCGAGTCATTCCGGGGCGC
CGCGAAGCGGCGAACCCGGA

Product: Enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MCDSDTASAPVLTIDGPRATIRLNRPKHLNRLQPDDLATLLDQFARLDADPAVRVLVLTGTGRAFSAGFDLGEVAERATA
EVEAESPGSAFEVVVNRLEDLRVPTICRLNGGVYGGSTDLALACDFRIGVDTAEMFMPAARLGLHYYPGGIKRYVSRLGI
DNAKRLFLTAQRIEAAEMLRIGYLTAMVPMDRLDREVDALATTLAGNAPNAVAGMKRAINEFARAAFDEQAADTRARASM
RSDEIKEGIAAFTEKRAPRF

Sequences:

>Translated_260_residues
MCDSDTASAPVLTIDGPRATIRLNRPKHLNRLQPDDLATLLDQFARLDADPAVRVLVLTGTGRAFSAGFDLGEVAERATA
EVEAESPGSAFEVVVNRLEDLRVPTICRLNGGVYGGSTDLALACDFRIGVDTAEMFMPAARLGLHYYPGGIKRYVSRLGI
DNAKRLFLTAQRIEAAEMLRIGYLTAMVPMDRLDREVDALATTLAGNAPNAVAGMKRAINEFARAAFDEQAADTRARASM
RSDEIKEGIAAFTEKRAPRF
>Mature_260_residues
MCDSDTASAPVLTIDGPRATIRLNRPKHLNRLQPDDLATLLDQFARLDADPAVRVLVLTGTGRAFSAGFDLGEVAERATA
EVEAESPGSAFEVVVNRLEDLRVPTICRLNGGVYGGSTDLALACDFRIGVDTAEMFMPAARLGLHYYPGGIKRYVSRLGI
DNAKRLFLTAQRIEAAEMLRIGYLTAMVPMDRLDREVDALATTLAGNAPNAVAGMKRAINEFARAAFDEQAADTRARASM
RSDEIKEGIAAFTEKRAPRF

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=30.8641975308642, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=26.5384615384615, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI37594469, Length=193, Percent_Identity=26.4248704663212, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI4502327, Length=245, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=242, Percent_Identity=28.5123966942149, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=30.3846153846154, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=28.1853281853282, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787660, Length=245, Percent_Identity=31.4285714285714, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=25.7028112449799, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=29.5454545454545, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=31.1475409836066, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=29.0983606557377, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=26.7716535433071, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=246, Percent_Identity=24.7967479674797, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=204, Percent_Identity=28.4313725490196, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=171, Percent_Identity=26.9005847953216, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=223, Percent_Identity=27.8026905829596, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=30.2788844621514, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=30.2788844621514, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24650670, Length=187, Percent_Identity=32.0855614973262, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357171, Length=242, Percent_Identity=26.0330578512397, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=27.6264591439689, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19920382, Length=229, Percent_Identity=24.8908296943231, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28204; Mature: 28204

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCDSDTASAPVLTIDGPRATIRLNRPKHLNRLQPDDLATLLDQFARLDADPAVRVLVLTG
CCCCCCCCCCEEEECCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEC
TGRAFSAGFDLGEVAERATAEVEAESPGSAFEVVVNRLEDLRVPTICRLNGGVYGGSTDL
CCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEECCCCCE
ALACDFRIGVDTAEMFMPAARLGLHYYPGGIKRYVSRLGIDNAKRLFLTAQRIEAAEMLR
EEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IGYLTAMVPMDRLDREVDALATTLAGNAPNAVAGMKRAINEFARAAFDEQAADTRARASM
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSDEIKEGIAAFTEKRAPRF
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCDSDTASAPVLTIDGPRATIRLNRPKHLNRLQPDDLATLLDQFARLDADPAVRVLVLTG
CCCCCCCCCCEEEECCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEC
TGRAFSAGFDLGEVAERATAEVEAESPGSAFEVVVNRLEDLRVPTICRLNGGVYGGSTDL
CCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEECCCCCE
ALACDFRIGVDTAEMFMPAARLGLHYYPGGIKRYVSRLGIDNAKRLFLTAQRIEAAEMLR
EEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IGYLTAMVPMDRLDREVDALATTLAGNAPNAVAGMKRAINEFARAAFDEQAADTRARASM
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSDEIKEGIAAFTEKRAPRF
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA