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The map label for this gene is nifH [H]

Identifier: 192290022

GI number: 192290022

Start: 1737371

End: 1738198

Strand: Reverse

Name: nifH [H]

Synonym: Rpal_1623

Alternate gene names: 192290022

Gene position: 1738198-1737371 (Counterclockwise)

Preceding gene: 192290023

Following gene: 192290021

Centisome position: 30.26

GC content: 61.47

Gene sequence:

>828_bases
ATGACCCGCAAGGTGGCGATCTACGGTAAGGGTGGCATCGGCAAGTCGACGACCACTCAGAATACGGCGGCGGCGCTCGC
TTTCTATCACGACAAGAACGTCTTCATTCACGGCTGCGATCCAAAGGCGGACTCGACCCGCCTGATCCTCGGCGGCAAGC
CGCAGGACACCGTGATGGACACGCTGCGCACGCTCGGCGCAGAGAAGGTCACGCTCGAAAACGTCGTGAAGAGCGGCTTC
AAGGACATCCGCTGCGTCGAGTCCGGCGGGCCCGAGCCCGGCGTCGGCTGCGCCGGCCGCGGCGTCATCACCGCGATCGA
CCTGATGGAAGCCAACAAGGCCTACACCGACGATCTCGATTTCATTTTCTTCGACGTGCTCGGCGACGTCGTGTGCGGCG
GCTTCGCGATGCCGATCCGCGACGGCAAGGCACAGGAAGTCTACATCGTGGCCTCCGGCGAGATGATGGCGATCTACGCC
GCCAACAACATCTGCAAGGGCCTCGTCAAATACGCCAAGCAGAGCGGCGTGCGACTCGGCGGCATCATCTGCAACAGCCG
CAAGGTCGACGGCGAGCGCGAATTCCTCGAAGAATTCACCGCGGCGATCGGCACCAAGATGATTCACTTCGTGCCGCGCG
ACAACATCGTGCAGAAGGCCGAATTCAACAAGAAGACCGTCACCGAATTCGATCCCGCCGCCGAGCAGGCCCATGAATAC
AAGCAGCTCGGCGCCGCCATCATCGACAACAAGGACTTCGTGATTCCGAAGCCTCTGACGATGGATCAGCTCGAAGCGAT
GGTCGTGAAGTACGGCCTGATGGACTGA

Upstream 100 bases:

>100_bases
TTGTCCAATGCGCACGCATTGGCACGTCCGTTGCTCTTAAGGATGGCGAGGCGATGGATCACTCATCGTCCCGATCAATT
CATCGCAGGAGCTTCGGACC

Downstream 100 bases:

>100_bases
CCTGCGCTCCCCGCGCCTGACGGCGTACTTCCCGCACGAGGACAATACAATGCCATATCACGAGTTCGACTGTTCGAAAT
GTCTGCCCGAACGCCAGAAG

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein 2; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTRKVAIYGKGGIGKSTTTQNTAAALAFYHDKNVFIHGCDPKADSTRLILGGKPQDTVMDTLRTLGAEKVTLENVVKSGF
KDIRCVESGGPEPGVGCAGRGVITAIDLMEANKAYTDDLDFIFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAIYA
ANNICKGLVKYAKQSGVRLGGIICNSRKVDGEREFLEEFTAAIGTKMIHFVPRDNIVQKAEFNKKTVTEFDPAAEQAHEY
KQLGAAIIDNKDFVIPKPLTMDQLEAMVVKYGLMD

Sequences:

>Translated_275_residues
MTRKVAIYGKGGIGKSTTTQNTAAALAFYHDKNVFIHGCDPKADSTRLILGGKPQDTVMDTLRTLGAEKVTLENVVKSGF
KDIRCVESGGPEPGVGCAGRGVITAIDLMEANKAYTDDLDFIFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAIYA
ANNICKGLVKYAKQSGVRLGGIICNSRKVDGEREFLEEFTAAIGTKMIHFVPRDNIVQKAEFNKKTVTEFDPAAEQAHEY
KQLGAAIIDNKDFVIPKPLTMDQLEAMVVKYGLMD
>Mature_274_residues
TRKVAIYGKGGIGKSTTTQNTAAALAFYHDKNVFIHGCDPKADSTRLILGGKPQDTVMDTLRTLGAEKVTLENVVKSGFK
DIRCVESGGPEPGVGCAGRGVITAIDLMEANKAYTDDLDFIFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAIYAA
NNICKGLVKYAKQSGVRLGGIICNSRKVDGEREFLEEFTAAIGTKMIHFVPRDNIVQKAEFNKKTVTEFDPAAEQAHEYK
QLGAAIIDNKDFVIPKPLTMDQLEAMVVKYGLMD

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein (component 2) and a component 1 which is either a molybdenum-iron protein, a vanadium-iron, or an iron-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 29833; Mature: 29702

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKVAIYGKGGIGKSTTTQNTAAALAFYHDKNVFIHGCDPKADSTRLILGGKPQDTVMD
CCCEEEEEECCCCCCCCCCCCHHEEEEEEECCCEEEECCCCCCCCCEEEECCCCHHHHHH
TLRTLGAEKVTLENVVKSGFKDIRCVESGGPEPGVGCAGRGVITAIDLMEANKAYTDDLD
HHHHCCCCHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCHH
FIFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLG
HHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCEEE
GIICNSRKVDGEREFLEEFTAAIGTKMIHFVPRDNIVQKAEFNKKTVTEFDPAAEQAHEY
EEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCCCCCCCCHHHHHHHHH
KQLGAAIIDNKDFVIPKPLTMDQLEAMVVKYGLMD
HHHCHHEECCCCEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRKVAIYGKGGIGKSTTTQNTAAALAFYHDKNVFIHGCDPKADSTRLILGGKPQDTVMD
CCEEEEEECCCCCCCCCCCCHHEEEEEEECCCEEEECCCCCCCCCEEEECCCCHHHHHH
TLRTLGAEKVTLENVVKSGFKDIRCVESGGPEPGVGCAGRGVITAIDLMEANKAYTDDLD
HHHHCCCCHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCHH
FIFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLG
HHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCEEE
GIICNSRKVDGEREFLEEFTAAIGTKMIHFVPRDNIVQKAEFNKKTVTEFDPAAEQAHEY
EEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCCCCCCCCHHHHHHHHH
KQLGAAIIDNKDFVIPKPLTMDQLEAMVVKYGLMD
HHHCHHEECCCCEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332060 [H]