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The map label for this gene is anfA [H]

Identifier: 192290023

GI number: 192290023

Start: 1738722

End: 1740407

Strand: Reverse

Name: anfA [H]

Synonym: Rpal_1624

Alternate gene names: 192290023

Gene position: 1740407-1738722 (Counterclockwise)

Preceding gene: 192290024

Following gene: 192290022

Centisome position: 30.3

GC content: 63.82

Gene sequence:

>1686_bases
ATGCCGATGGCCCAAAATTCCCGCCGAGCTCCTGCCGCCCCCATCGCGCCCGAGCCCCCCAAACGCCTCGCGACGCTGGA
CCTCAGCGGCGAACCGGAAGGTTTCGTCGACGAATTCACCCACTGCTTCACCGGCGAATGCCGGGTCAATGTGTTGCAGA
TCCTTTTTCGCATCAATCAGGTGCTGACGCAGAACGCTGACCTCGCCACGCTGGTGTCGATCATTCTCGACGGCATGCGC
CAGCAGATGCGGATGCAGCGCGGCGTGATGATGCTGTACGATCGCCACTCCGACGCGATCTTCATCCACGACAGCTTCGG
ACTGACCGAGGAGGAGCGCGGCCGCGGCATCTACGCGCCCGGCGAAGGAATCACCGGCAAGGTGGTCGAGACCGGCAAGC
CGATCATCATCCCGCGGGTGATCGACAGCCCGGACTTTCTCGATCGCACCCGCGCCCACAACAAGGGGCGCAAGCAGGAC
AAGCTGGCGTTCTTCTGCGTGCCGATCGTGCTCGCCCAGAAGGTGCTCGGCACCATCTGCGCCGAGCGCGTCTATATGAA
TCAGCGGCTGCTGAATCAGGATGCCGAACTGCTGGCGATGGTCGCCTCGCTGATCGCACCGGCGGTCGAGCTGTATCTGA
TCGAGAACGTCGACAAGGTGCGGCTGGAGACCGAGAACAGGCGGCTGAAAAGCGAGCTGAAGCAGCGCTTTCGCCCGGCC
AACATCATCGGCAACTCCAAGCCGATGCAGGAAGTCTACGCCATGGTGCACAAGGTGGCCTCGACCAAGGCCACCGTGCT
GCTGCTCGGCGAAAGCGGCGTCGGCAAGGAGCTGGTGGCGAGCGCGCTGCACTACAACAGCCCAGTGGCCGACGGCCCGT
TCATCAAGGCGAACTGCGCTGCGCTGCCCGAAGCGCTGGCGGAGAGCGAGCTGTTCGGCCACGAGCGCGGCGCGTTCACC
AGCGCGATCGCCACTCACAAGGGTTACTTCGAACAGGCATCTGGCGGCACGATCTTTCTCGACGAGGTCGGCGAATTAAG
TCTACCGACGCAGGCCAAGCTGCTGCGCGTACTGCAGGAACGGACGTTCGAGCGCGTCGGCGGCGCCAAGCCGGTCAAAG
TCGATGTCCGGATCATCGCCGCCACCAACCGCAATCTCGCCGAGATGGTCGCTGAGGGCACCTTCCGCGAAGATCTGTTC
TATCGCCTCAACGTCTTCCCGATCACCATCCCGCCGCTGCGCGATCGCGGCTCGGACGTGATCACCCTCGCGGACCACTT
CGTCACCACCTATTCCGCCGAAATCGGCAAACCGATCAAACGGATCTCGACGCCTGCGATCAACATGCTGATGAGCTATC
ACTGGCCCGGCAACGTCCGCGAGCTGGAGAACGTGATCGAGCGATCGGTGATCCTGGCGGAGGAAGGCGTGATCCACGGC
TACGATCTGCCGCCGTCGCTGCAGACGCCGACCGAAACCGGGACCGGCTTCAGTGGCACGCTCGAAGACCGCGTCACGGC
AGTCGAATACGAGATGATCGTCGAGGCGCTCAAAGCCTCGAATGGCAATGTCGGCCAGGCCGCCACCACGCTCGGCCTGA
CGCGGCGAATGCTCGGCCTGCGGATGGAGCGCCACGGACTGACCTACAAGACATTCCGCACCGCAGGGCTGCGCCCGCGG
AACTGA

Upstream 100 bases:

>100_bases
GCTGGTGCGCTACGAGCTGTCCAGCGGCGAGCTGCTGCTGCAACTCAGCGGCATCAGCACTTCTACGATCACGCTGACGA
TCCTGCCGGTGGAGTGAGCG

Downstream 100 bases:

>100_bases
CGTCGTCTCGCCATTCGCTTCGGAATCTCAGACGGCGGCTCTTACGAGCCGCCGTTTTGCGTTGGCGGAGAGCTCCCGCG
CAATCAACCGCCGGGTAAAT

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MPMAQNSRRAPAAPIAPEPPKRLATLDLSGEPEGFVDEFTHCFTGECRVNVLQILFRINQVLTQNADLATLVSIILDGMR
QQMRMQRGVMMLYDRHSDAIFIHDSFGLTEEERGRGIYAPGEGITGKVVETGKPIIIPRVIDSPDFLDRTRAHNKGRKQD
KLAFFCVPIVLAQKVLGTICAERVYMNQRLLNQDAELLAMVASLIAPAVELYLIENVDKVRLETENRRLKSELKQRFRPA
NIIGNSKPMQEVYAMVHKVASTKATVLLLGESGVGKELVASALHYNSPVADGPFIKANCAALPEALAESELFGHERGAFT
SAIATHKGYFEQASGGTIFLDEVGELSLPTQAKLLRVLQERTFERVGGAKPVKVDVRIIAATNRNLAEMVAEGTFREDLF
YRLNVFPITIPPLRDRGSDVITLADHFVTTYSAEIGKPIKRISTPAINMLMSYHWPGNVRELENVIERSVILAEEGVIHG
YDLPPSLQTPTETGTGFSGTLEDRVTAVEYEMIVEALKASNGNVGQAATTLGLTRRMLGLRMERHGLTYKTFRTAGLRPR
N

Sequences:

>Translated_561_residues
MPMAQNSRRAPAAPIAPEPPKRLATLDLSGEPEGFVDEFTHCFTGECRVNVLQILFRINQVLTQNADLATLVSIILDGMR
QQMRMQRGVMMLYDRHSDAIFIHDSFGLTEEERGRGIYAPGEGITGKVVETGKPIIIPRVIDSPDFLDRTRAHNKGRKQD
KLAFFCVPIVLAQKVLGTICAERVYMNQRLLNQDAELLAMVASLIAPAVELYLIENVDKVRLETENRRLKSELKQRFRPA
NIIGNSKPMQEVYAMVHKVASTKATVLLLGESGVGKELVASALHYNSPVADGPFIKANCAALPEALAESELFGHERGAFT
SAIATHKGYFEQASGGTIFLDEVGELSLPTQAKLLRVLQERTFERVGGAKPVKVDVRIIAATNRNLAEMVAEGTFREDLF
YRLNVFPITIPPLRDRGSDVITLADHFVTTYSAEIGKPIKRISTPAINMLMSYHWPGNVRELENVIERSVILAEEGVIHG
YDLPPSLQTPTETGTGFSGTLEDRVTAVEYEMIVEALKASNGNVGQAATTLGLTRRMLGLRMERHGLTYKTFRTAGLRPR
N
>Mature_560_residues
PMAQNSRRAPAAPIAPEPPKRLATLDLSGEPEGFVDEFTHCFTGECRVNVLQILFRINQVLTQNADLATLVSIILDGMRQ
QMRMQRGVMMLYDRHSDAIFIHDSFGLTEEERGRGIYAPGEGITGKVVETGKPIIIPRVIDSPDFLDRTRAHNKGRKQDK
LAFFCVPIVLAQKVLGTICAERVYMNQRLLNQDAELLAMVASLIAPAVELYLIENVDKVRLETENRRLKSELKQRFRPAN
IIGNSKPMQEVYAMVHKVASTKATVLLLGESGVGKELVASALHYNSPVADGPFIKANCAALPEALAESELFGHERGAFTS
AIATHKGYFEQASGGTIFLDEVGELSLPTQAKLLRVLQERTFERVGGAKPVKVDVRIIAATNRNLAEMVAEGTFREDLFY
RLNVFPITIPPLRDRGSDVITLADHFVTTYSAEIGKPIKRISTPAINMLMSYHWPGNVRELENVIERSVILAEEGVIHGY
DLPPSLQTPTETGTGFSGTLEDRVTAVEYEMIVEALKASNGNVGQAATTLGLTRRMLGLRMERHGLTYKTFRTAGLRPRN

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=332, Percent_Identity=49.0963855421687, Blast_Score=328, Evalue=7e-91,
Organism=Escherichia coli, GI87082117, Length=378, Percent_Identity=44.4444444444444, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1789087, Length=403, Percent_Identity=43.1761786600496, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1790437, Length=303, Percent_Identity=45.5445544554455, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1790299, Length=330, Percent_Identity=42.4242424242424, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI87082152, Length=463, Percent_Identity=36.2850971922246, Blast_Score=245, Evalue=6e-66,
Organism=Escherichia coli, GI1788905, Length=320, Percent_Identity=42.8125, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=40.7523510971787, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=41.7956656346749, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1787583, Length=319, Percent_Identity=38.5579937304075, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1786524, Length=330, Percent_Identity=38.4848484848485, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=33, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789828, Length=277, Percent_Identity=34.6570397111913, Blast_Score=149, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 62077; Mature: 61945

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMAQNSRRAPAAPIAPEPPKRLATLDLSGEPEGFVDEFTHCFTGECRVNVLQILFRINQ
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
VLTQNADLATLVSIILDGMRQQMRMQRGVMMLYDRHSDAIFIHDSFGLTEEERGRGIYAP
HHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCHHHCCCEEECC
GEGITGKVVETGKPIIIPRVIDSPDFLDRTRAHNKGRKQDKLAFFCVPIVLAQKVLGTIC
CCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AERVYMNQRLLNQDAELLAMVASLIAPAVELYLIENVDKVRLETENRRLKSELKQRFRPA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEHHHHHHHHHHHHHCCCC
NIIGNSKPMQEVYAMVHKVASTKATVLLLGESGVGKELVASALHYNSPVADGPFIKANCA
CCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEECCHH
ALPEALAESELFGHERGAFTSAIATHKGYFEQASGGTIFLDEVGELSLPTQAKLLRVLQE
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
RTFERVGGAKPVKVDVRIIAATNRNLAEMVAEGTFREDLFYRLNVFPITIPPLRDRGSDV
HHHHHHCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHEEEEEEEEEECCCCCCCCCCE
ITLADHFVTTYSAEIGKPIKRISTPAINMLMSYHWPGNVRELENVIERSVILAEEGVIHG
EEEHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCEEEC
YDLPPSLQTPTETGTGFSGTLEDRVTAVEYEMIVEALKASNGNVGQAATTLGLTRRMLGL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RMERHGLTYKTFRTAGLRPRN
HHHHCCCEEEHHHHCCCCCCC
>Mature Secondary Structure 
PMAQNSRRAPAAPIAPEPPKRLATLDLSGEPEGFVDEFTHCFTGECRVNVLQILFRINQ
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
VLTQNADLATLVSIILDGMRQQMRMQRGVMMLYDRHSDAIFIHDSFGLTEEERGRGIYAP
HHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCHHHCCCEEECC
GEGITGKVVETGKPIIIPRVIDSPDFLDRTRAHNKGRKQDKLAFFCVPIVLAQKVLGTIC
CCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AERVYMNQRLLNQDAELLAMVASLIAPAVELYLIENVDKVRLETENRRLKSELKQRFRPA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEHHHHHHHHHHHHHCCCC
NIIGNSKPMQEVYAMVHKVASTKATVLLLGESGVGKELVASALHYNSPVADGPFIKANCA
CCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEECCHH
ALPEALAESELFGHERGAFTSAIATHKGYFEQASGGTIFLDEVGELSLPTQAKLLRVLQE
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
RTFERVGGAKPVKVDVRIIAATNRNLAEMVAEGTFREDLFYRLNVFPITIPPLRDRGSDV
HHHHHHCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHEEEEEEEEEECCCCCCCCCCE
ITLADHFVTTYSAEIGKPIKRISTPAINMLMSYHWPGNVRELENVIERSVILAEEGVIHG
EEEHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCEEEC
YDLPPSLQTPTETGTGFSGTLEDRVTAVEYEMIVEALKASNGNVGQAATTLGLTRRMLGL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RMERHGLTYKTFRTAGLRPRN
HHHHCCCEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]