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The map label for this gene is nodV [H]

Identifier: 192289927

GI number: 192289927

Start: 1604215

End: 1605057

Strand: Reverse

Name: nodV [H]

Synonym: Rpal_1523

Alternate gene names: 192289927

Gene position: 1605057-1604215 (Counterclockwise)

Preceding gene: 192289928

Following gene: 192289912

Centisome position: 27.94

GC content: 63.58

Gene sequence:

>843_bases
ATGCGAGAACCGCCAGCTTCGGCCCCCTGCACGTCCCGCACCCCTCAGATGTGCTGGTCCGGCGAAAATTCGGCCCTGAG
CGACCTCCGCTTGTCACCTGACAGCCGCAGTTGCAATGGCATCGGCGATGCGGTGAAGCCTGTGAATGCGACCAGGTCCG
ACGAAATGGCGGCCGTTGGACATTATTCCGCGGTGATGTCGCGGGAGCTCCATCATCCACTCACCAGCCTGGTGTTCGAT
GCGGAGGCGGCGCTTCGCTGGCTCGACCGGCCGCAAGCCGAGCTCGGGGAAGTCATCCGGGGTCTGGAACGCATGCGTGA
CGCGGCGCTTCGTGCCGTCGCCATAGCACGGTCCTTGCAAGCGCTGGCTGTTCACGCCGCACCGTCATTTGCGCCCGTTC
TGATCGAGGATCTGCTTGAAGCCATGCTGAGGAACGTCGCCAGGGATATCGAACGGCATCGGATCGCGGTCGAAACCGAG
CTCCCTGCGGTACGCAGTTTGGTGAGAGCCGATCCCGTGCAAATCCAACAGGTCCTGCTCAACCTCATCGGCAACGCAAT
CGACGCGATGGCCGTCACCGAAGGCAGAGACCGACGCCTGTCGATCGCTATCCGGCATGTCGGCAGCGCGGTCCATTGCA
GCATCAGCGATACTGGAACAGGAATGGAAGCGGACACCGTGGCTCGCGTGTTCGAGCCGCTCTTCTCGACCCGTCCGGCC
AAGAAAGGCCTCGGCCTGACGATCGCGCGCGCCATCATCGAAGCGCATGGCGGCAAGCTCGAGGCGCGCTCCCGGCCTGG
GATCGGCAGCACGCTCTCGTTTCATCTCGATGTGACACGCTGA

Upstream 100 bases:

>100_bases
CGTTCAAGACGCAAGGTTCTGTGCCGAGCATCATCACGATGGAGGAAAGGCTGCCGGCGGAGACGTCGGCGCGCCGCTCC
GATTTCGAAGGAATGCAAGG

Downstream 100 bases:

>100_bases
CCTCTGCACCACCGAAGGTCCTGGAGGCAGCCAAAAGTTGGCGACCAAACGTCTTCGGCCGGGAAGTGCTTCAACAGACT
AGCAGCCGGCGGAGCACCAA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MREPPASAPCTSRTPQMCWSGENSALSDLRLSPDSRSCNGIGDAVKPVNATRSDEMAAVGHYSAVMSRELHHPLTSLVFD
AEAALRWLDRPQAELGEVIRGLERMRDAALRAVAIARSLQALAVHAAPSFAPVLIEDLLEAMLRNVARDIERHRIAVETE
LPAVRSLVRADPVQIQQVLLNLIGNAIDAMAVTEGRDRRLSIAIRHVGSAVHCSISDTGTGMEADTVARVFEPLFSTRPA
KKGLGLTIARAIIEAHGGKLEARSRPGIGSTLSFHLDVTR

Sequences:

>Translated_280_residues
MREPPASAPCTSRTPQMCWSGENSALSDLRLSPDSRSCNGIGDAVKPVNATRSDEMAAVGHYSAVMSRELHHPLTSLVFD
AEAALRWLDRPQAELGEVIRGLERMRDAALRAVAIARSLQALAVHAAPSFAPVLIEDLLEAMLRNVARDIERHRIAVETE
LPAVRSLVRADPVQIQQVLLNLIGNAIDAMAVTEGRDRRLSIAIRHVGSAVHCSISDTGTGMEADTVARVFEPLFSTRPA
KKGLGLTIARAIIEAHGGKLEARSRPGIGSTLSFHLDVTR
>Mature_280_residues
MREPPASAPCTSRTPQMCWSGENSALSDLRLSPDSRSCNGIGDAVKPVNATRSDEMAAVGHYSAVMSRELHHPLTSLVFD
AEAALRWLDRPQAELGEVIRGLERMRDAALRAVAIARSLQALAVHAAPSFAPVLIEDLLEAMLRNVARDIERHRIAVETE
LPAVRSLVRADPVQIQQVLLNLIGNAIDAMAVTEGRDRRLSIAIRHVGSAVHCSISDTGTGMEADTVARVFEPLFSTRPA
KKGLGLTIARAIIEAHGGKLEARSRPGIGSTLSFHLDVTR

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process. NodV may function as a membrane-associated protein kinase that phosphorylates nodW in response to

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=28.5087719298246, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=26.1802575107296, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790300, Length=134, Percent_Identity=35.0746268656716, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 30222; Mature: 30222

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREPPASAPCTSRTPQMCWSGENSALSDLRLSPDSRSCNGIGDAVKPVNATRSDEMAAVG
CCCCCCCCCCCCCCCHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHH
HYSAVMSRELHHPLTSLVFDAEAALRWLDRPQAELGEVIRGLERMRDAALRAVAIARSLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAVHAAPSFAPVLIEDLLEAMLRNVARDIERHRIAVETELPAVRSLVRADPVQIQQVLL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCHHHHHHHHH
NLIGNAIDAMAVTEGRDRRLSIAIRHVGSAVHCSISDTGTGMEADTVARVFEPLFSTRPA
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
KKGLGLTIARAIIEAHGGKLEARSRPGIGSTLSFHLDVTR
CCCCHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MREPPASAPCTSRTPQMCWSGENSALSDLRLSPDSRSCNGIGDAVKPVNATRSDEMAAVG
CCCCCCCCCCCCCCCHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHH
HYSAVMSRELHHPLTSLVFDAEAALRWLDRPQAELGEVIRGLERMRDAALRAVAIARSLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAVHAAPSFAPVLIEDLLEAMLRNVARDIERHRIAVETELPAVRSLVRADPVQIQQVLL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCHHHHHHHHH
NLIGNAIDAMAVTEGRDRRLSIAIRHVGSAVHCSISDTGTGMEADTVARVFEPLFSTRPA
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
KKGLGLTIARAIIEAHGGKLEARSRPGIGSTLSFHLDVTR
CCCCHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]