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The map label for this gene is ispB [H]

Identifier: 192289642

GI number: 192289642

Start: 1304398

End: 1305408

Strand: Reverse

Name: ispB [H]

Synonym: Rpal_1232

Alternate gene names: 192289642

Gene position: 1305408-1304398 (Counterclockwise)

Preceding gene: 192289651

Following gene: 192289638

Centisome position: 22.73

GC content: 65.97

Gene sequence:

>1011_bases
GTGGCCGTTATCGTACCCTTCGAGGGCCCCTCGACCGCCTCGATCGACCAACTGGTCGAACTCGTTGCGGCGGACATGGA
GCGCGTCAACGCCACCATCCTGTCGCGGACCGGCTCCGACGTCACCATGATTCCGGAAGTCGCCAACCATCTGATCTCTT
CCGGCGGCAAGCGGCTGCGGCCGATGCTGACTTTGGCGATGGCGAATCTCACCGGTTACACCGGCGACGGCCACATCAAG
CTCGCTGCCGCAGTCGAATTCATGCACACCGCGACGCTGCTGCACGACGACGTCGTCGACGAGAGCGAGATGCGCCGCGG
CAAGAAGTCGGCGCGGATGCTGTGGGGTAACGAGGCCAGCGTGCTGGTCGGCGACTTCCTGCTCGGCCAGGCGTTCCGGA
TGATGGTCGAGGTCGGCTCGCTGCGCGCACTCGACATCCTGTCGTCGGCGTCCGCCACCATCGCCGAAGGCGAGGTGATG
CAGCTTGCCGCCGCCAAGAACACCGCAACCACCGAAGACGAATATCTCGCGGTGATCCGCGGCAAGACCGCCGAACTGTT
CGCCGCCGCCTGCGAAGTCGGCCCGGCGATTGCCAACCGGCCGAAGCCGGAGCAGTCGGCCTGCCGCTCGTTCGGCATGA
ACATCGGCATCGCCTTCCAGCTGATCGACGACGTGCTCGACTACGGCGGCAAGGCCGCCAAGCTCGGCAAGAACGTCGGC
GACGACTTCCGTGAGGGCAAGATCACCCTGCCGGTTGTGCTGGCGTTCCGCCGCGGCAACGACGCCGAGCGCGAGTTCTG
GATCAAGTCGCTGGAGCGCGGCGAGATTTCGGATGCCGACCTCGATCAGGCGATCAAGCTGATGACCAAGCACCGCGCAC
TCGACGACACCATCCAGCGTGCCCATCACTACGGCGCGATGGCGGTCGACGCGCTGGCGCTGTTCCCCTCCTCGCCGATG
AAGACGGCGCTGGAGCAGGTGGTCGCGTTCTGCCTCGCCCGCTCACACTGA

Upstream 100 bases:

>100_bases
CGCGGAATGGTCGAGCCGGCCGGCGCCTCGATGACTGGACCAAGGCGCGGATGGCGCGAGCAGCTTCGCCGCATCCGTCC
CCGGAATTTGGAGAGCCAGC

Downstream 100 bases:

>100_bases
GCGGACGCAGCTCTTCCTCAAACAAGCAACGTCATTCCGGGGGCGCTCGCAAAGCGAGCGAACCCGGAATGCCGAGATGT
TCTGAACTGCGAGATCCCGG

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MAVIVPFEGPSTASIDQLVELVAADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLRPMLTLAMANLTGYTGDGHIK
LAAAVEFMHTATLLHDDVVDESEMRRGKKSARMLWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSSASATIAEGEVM
QLAAAKNTATTEDEYLAVIRGKTAELFAAACEVGPAIANRPKPEQSACRSFGMNIGIAFQLIDDVLDYGGKAAKLGKNVG
DDFREGKITLPVVLAFRRGNDAEREFWIKSLERGEISDADLDQAIKLMTKHRALDDTIQRAHHYGAMAVDALALFPSSPM
KTALEQVVAFCLARSH

Sequences:

>Translated_336_residues
MAVIVPFEGPSTASIDQLVELVAADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLRPMLTLAMANLTGYTGDGHIK
LAAAVEFMHTATLLHDDVVDESEMRRGKKSARMLWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSSASATIAEGEVM
QLAAAKNTATTEDEYLAVIRGKTAELFAAACEVGPAIANRPKPEQSACRSFGMNIGIAFQLIDDVLDYGGKAAKLGKNVG
DDFREGKITLPVVLAFRRGNDAEREFWIKSLERGEISDADLDQAIKLMTKHRALDDTIQRAHHYGAMAVDALALFPSSPM
KTALEQVVAFCLARSH
>Mature_335_residues
AVIVPFEGPSTASIDQLVELVAADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLRPMLTLAMANLTGYTGDGHIKL
AAAVEFMHTATLLHDDVVDESEMRRGKKSARMLWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSSASATIAEGEVMQ
LAAAKNTATTEDEYLAVIRGKTAELFAAACEVGPAIANRPKPEQSACRSFGMNIGIAFQLIDDVLDYGGKAAKLGKNVGD
DFREGKITLPVVLAFRRGNDAEREFWIKSLERGEISDADLDQAIKLMTKHRALDDTIQRAHHYGAMAVDALALFPSSPMK
TALEQVVAFCLARSH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=303, Percent_Identity=28.7128712871287, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI169808399, Length=263, Percent_Identity=24.3346007604563, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4758430, Length=211, Percent_Identity=23.2227488151659, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI83700220, Length=211, Percent_Identity=23.2227488151659, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789578, Length=311, Percent_Identity=44.0514469453376, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1786623, Length=225, Percent_Identity=31.1111111111111, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=319, Percent_Identity=28.8401253918495, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=307, Percent_Identity=29.6416938110749, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325188, Length=232, Percent_Identity=22.8448275862069, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24651612, Length=309, Percent_Identity=29.126213592233, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI281365769, Length=203, Percent_Identity=24.6305418719212, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24660002, Length=203, Percent_Identity=24.6305418719212, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36218; Mature: 36087

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIVPFEGPSTASIDQLVELVAADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLR
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCHHHH
PMLTLAMANLTGYTGDGHIKLAAAVEFMHTATLLHDDVVDESEMRRGKKSARMLWGNEAS
HHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHEECCCCCH
VLVGDFLLGQAFRMMVEVGSLRALDILSSASATIAEGEVMQLAAAKNTATTEDEYLAVIR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHCCCHHHHHHHHCCCCCCCHHEEEEEC
GKTAELFAAACEVGPAIANRPKPEQSACRSFGMNIGIAFQLIDDVLDYGGKAAKLGKNVG
CCHHHHHHHHHHHCHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCC
DDFREGKITLPVVLAFRRGNDAEREFWIKSLERGEISDADLDQAIKLMTKHRALDDTIQR
CHHHCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AHHYGAMAVDALALFPSSPMKTALEQVVAFCLARSH
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVIVPFEGPSTASIDQLVELVAADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCHHHH
PMLTLAMANLTGYTGDGHIKLAAAVEFMHTATLLHDDVVDESEMRRGKKSARMLWGNEAS
HHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHEECCCCCH
VLVGDFLLGQAFRMMVEVGSLRALDILSSASATIAEGEVMQLAAAKNTATTEDEYLAVIR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHCCCHHHHHHHHCCCCCCCHHEEEEEC
GKTAELFAAACEVGPAIANRPKPEQSACRSFGMNIGIAFQLIDDVLDYGGKAAKLGKNVG
CCHHHHHHHHHHHCHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCC
DDFREGKITLPVVLAFRRGNDAEREFWIKSLERGEISDADLDQAIKLMTKHRALDDTIQR
CHHHCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AHHYGAMAVDALALFPSSPMKTALEQVVAFCLARSH
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]