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The map label for this gene is smeIM [H]

Identifier: 192289628

GI number: 192289628

Start: 1287967

End: 1289097

Strand: Reverse

Name: smeIM [H]

Synonym: Rpal_1218

Alternate gene names: 192289628

Gene position: 1289097-1287967 (Counterclockwise)

Preceding gene: 192289633

Following gene: 192289620

Centisome position: 22.44

GC content: 62.95

Gene sequence:

>1131_bases
ATGGTTGTGTCGCGTCGCGGGGCGTCTGCAAGGGCGCCCCGCACTCAATTTGAAGATATGCCCGAGAGCCGAATCATCGT
CGGTGATTGCATCGCCGAGATGGAGAAGCTCCCGCCGAAGTCGGTGGATCTGGTGTTCGCCGATCCGCCGTACAATCTGC
AGCTCAAAGGTGCGCTGAAACGTCCCGACGAATCGGAAGTCGACGCCGTCGACGACGATTGGGACAAGTTCGCGTCCTTC
GCCGCTTACGACAACTTCACCCGTGCGTGGCTGCTCGCCGCGCGACGGATCATGAAGCCGTCGGCGACACTGTGGGTGAT
CGGCTCGTACCACAACATTTTCCGCGTCGGCACGATCATGCAGGACCTCGGGTTCTGGGTGCTCAACGACATCGTTTGGC
GCAAGTCCAATCCGATGCCGAACTTCCGCGGCCGCCGTTTCACCAATGCGCACGAAACCATGATCTGGGCCGCGCGCGAC
GAGAACGCCAAGGGCTACACCTTCAACTACGATGCGTTGAAGGCCGCCAACGAGGACGTTCAGGCGCGCTCGGACTGGCT
GATTCCGCTGTGCACCGGCGACGAACGGTTGAAGGGCAAGGACGGCAAGAAGGTGCACCCGACGCAGAAGCCCGAACAGC
TTCTGGCGCGCGTGCTGTTGAGCTCGTCGAAGCCGGGCGATCTGGTGGTCGATCCGTTCAACGGCACCGGCACCACAGGT
GCTGTCGCCAAGCGTTTGCGCCGCAACTACATCGGCATCGAGCGCGACAAGACCTACGCCGAAGCTGCACGTGCGCGGAT
CGATTCGATCGAACCGCTGCCGGACGACACGCTGAAGCCTTTCCTCACCGCGCGCGATGCGCCGCGAGTGGCGTTCTCCG
AGCTGATCGAGCGCGGCATGATTTCGCCGGGCGCACGATTAGTCGATTCGAAGAAGCGGCACGGCGCACTGGTGCGCGCC
GACGGCGCCATCATGCTCGGCGACAAGGTCGGATCGATTCACCGAATCGGTGCAGTGGCCCAGGGGTCGGAGGCCTGCAA
CGGCTGGACGTTCTGGCACGTCGAAACCAGCAAGGGTCTGCGGCTGATCGACGAACTCCGCGCCGAGATTCGCAGCACGA
TGATCGCCTGA

Upstream 100 bases:

>100_bases
CAATTAAGTCGATTTTTACTTTGACGGGTCATTGTAGTGGCTAGTCGGTTTGTTGGTCCCGCGTCGCCGACGGCGTCACA
TGAGTCAGGTTAGTTGAGTC

Downstream 100 bases:

>100_bases
CGCAAATAAGTTTCTCCGGTTCTGATCCAATCAGAACCGGAGCTCTTTTGGCCGTGGTTGCGATATGCGAACCACGCCTG
CTTTCCCTAAGACATCTCCC

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MVVSRRGASARAPRTQFEDMPESRIIVGDCIAEMEKLPPKSVDLVFADPPYNLQLKGALKRPDESEVDAVDDDWDKFASF
AAYDNFTRAWLLAARRIMKPSATLWVIGSYHNIFRVGTIMQDLGFWVLNDIVWRKSNPMPNFRGRRFTNAHETMIWAARD
ENAKGYTFNYDALKAANEDVQARSDWLIPLCTGDERLKGKDGKKVHPTQKPEQLLARVLLSSSKPGDLVVDPFNGTGTTG
AVAKRLRRNYIGIERDKTYAEAARARIDSIEPLPDDTLKPFLTARDAPRVAFSELIERGMISPGARLVDSKKRHGALVRA
DGAIMLGDKVGSIHRIGAVAQGSEACNGWTFWHVETSKGLRLIDELRAEIRSTMIA

Sequences:

>Translated_376_residues
MVVSRRGASARAPRTQFEDMPESRIIVGDCIAEMEKLPPKSVDLVFADPPYNLQLKGALKRPDESEVDAVDDDWDKFASF
AAYDNFTRAWLLAARRIMKPSATLWVIGSYHNIFRVGTIMQDLGFWVLNDIVWRKSNPMPNFRGRRFTNAHETMIWAARD
ENAKGYTFNYDALKAANEDVQARSDWLIPLCTGDERLKGKDGKKVHPTQKPEQLLARVLLSSSKPGDLVVDPFNGTGTTG
AVAKRLRRNYIGIERDKTYAEAARARIDSIEPLPDDTLKPFLTARDAPRVAFSELIERGMISPGARLVDSKKRHGALVRA
DGAIMLGDKVGSIHRIGAVAQGSEACNGWTFWHVETSKGLRLIDELRAEIRSTMIA
>Mature_376_residues
MVVSRRGASARAPRTQFEDMPESRIIVGDCIAEMEKLPPKSVDLVFADPPYNLQLKGALKRPDESEVDAVDDDWDKFASF
AAYDNFTRAWLLAARRIMKPSATLWVIGSYHNIFRVGTIMQDLGFWVLNDIVWRKSNPMPNFRGRRFTNAHETMIWAARD
ENAKGYTFNYDALKAANEDVQARSDWLIPLCTGDERLKGKDGKKVHPTQKPEQLLARVLLSSSKPGDLVVDPFNGTGTTG
AVAKRLRRNYIGIERDKTYAEAARARIDSIEPLPDDTLKPFLTARDAPRVAFSELIERGMISPGARLVDSKKRHGALVRA
DGAIMLGDKVGSIHRIGAVAQGSEACNGWTFWHVETSKGLRLIDELRAEIRSTMIA

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=263, Percent_Identity=31.5589353612167, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 42039; Mature: 42039

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVSRRGASARAPRTQFEDMPESRIIVGDCIAEMEKLPPKSVDLVFADPPYNLQLKGALK
CCCCCCCCCCCCCCCHHHCCCCCEEEHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCC
RPDESEVDAVDDDWDKFASFAAYDNFTRAWLLAARRIMKPSATLWVIGSYHNIFRVGTIM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
QDLGFWVLNDIVWRKSNPMPNFRGRRFTNAHETMIWAARDENAKGYTFNYDALKAANEDV
HHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCEEEEHHHHHHCCCCH
QARSDWLIPLCTGDERLKGKDGKKVHPTQKPEQLLARVLLSSSKPGDLVVDPFNGTGTTG
HCCCCCEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
AVAKRLRRNYIGIERDKTYAEAARARIDSIEPLPDDTLKPFLTARDAPRVAFSELIERGM
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCC
ISPGARLVDSKKRHGALVRADGAIMLGDKVGSIHRIGAVAQGSEACNGWTFWHVETSKGL
CCCCHHHHCCHHHCCCEEEECCEEEECCCCCCHHHHCCHHCCCCCCCCEEEEEEECCCCH
RLIDELRAEIRSTMIA
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVSRRGASARAPRTQFEDMPESRIIVGDCIAEMEKLPPKSVDLVFADPPYNLQLKGALK
CCCCCCCCCCCCCCCHHHCCCCCEEEHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCC
RPDESEVDAVDDDWDKFASFAAYDNFTRAWLLAARRIMKPSATLWVIGSYHNIFRVGTIM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
QDLGFWVLNDIVWRKSNPMPNFRGRRFTNAHETMIWAARDENAKGYTFNYDALKAANEDV
HHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCEEEEHHHHHHCCCCH
QARSDWLIPLCTGDERLKGKDGKKVHPTQKPEQLLARVLLSSSKPGDLVVDPFNGTGTTG
HCCCCCEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
AVAKRLRRNYIGIERDKTYAEAARARIDSIEPLPDDTLKPFLTARDAPRVAFSELIERGM
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCC
ISPGARLVDSKKRHGALVRADGAIMLGDKVGSIHRIGAVAQGSEACNGWTFWHVETSKGL
CCCCHHHHCCHHHCCCEEEECCEEEECCCCCCHHHHCCHHCCCCCCCCEEEEEEECCCCH
RLIDELRAEIRSTMIA
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]