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The map label for this gene is yhcR [H]

Identifier: 192289452

GI number: 192289452

Start: 1088648

End: 1090330

Strand: Reverse

Name: yhcR [H]

Synonym: Rpal_1025

Alternate gene names: 192289452

Gene position: 1090330-1088648 (Counterclockwise)

Preceding gene: 192289454

Following gene: 192289451

Centisome position: 18.98

GC content: 65.83

Gene sequence:

>1683_bases
ATGATCAAGCTCCGCTCCCGCCTGGCCCTGCTCGCCACCGCCGCCGCGCTCACTGTCGCATTCGTGCCCGCACCGCGCGC
GGCCGAATTGCTGCCGCCGCCGGTCGATCTGCGGATTCTCGCGATCAACGACTTCCACGGCAATTTGCAGCCACCGCCCG
GCGGAATCGCGATTGACGATCCGGCCGATCGGACCAAGAAGATTCACGTCCCCGCCGGCGGCGCAGAACATATGGCGACG
CTGGTGAAGGCGCTGCGCAAGGATCATCCCAACGCGATCTTCGTCGCCGCCGGCGATCTGATCGGCGCCAGCCCGTTTCT
GTCGGCGATGTTTCACGACGAGCCGACCATCGAGTCGCTGTCGCTGATGGGGCTGGCGCTATCCTCGGTCGGCAATCACG
AATTCGACGAGGGCAAGACCGAGCTGTTGCGGATGCAGAACGGCGGCTGCCATCCGGTCGACGGCTGCCAGGGGCCGCAT
CCGTTCAAGGGCGCGGCGTTCAACTATCTGGCCGCCTCCACGGTCGAGACCGCGACCGGCAAGACGGTGTTTCCGCCCTA
TGCGATCCGCAAGTTCAGCGGCGTGCCGGTGGCGTTCATCGGGCTGACGCTGAAGAACACGCCCAACATGGTGTCACCGC
CGGGGGTCGCCGGACTGTCGTTCAAGGACGAGGCCGAGACCGTCAACGCCCTGATCCCGGAGCTGAAGGCCAAGGGCGTC
GAGGCGATCGTGGTGCTAATCCATGAAGGTGGTTTTCCGACCGGCGACTACAACGAATGCCCGGGCATCTCCGGCCCGAT
CGTCGAGATCGTCAACAAGCTCGATCGCGCCGTCGACGTGGTGATCAGCGGCCACACCCACCGCGCCTACACCTGCCGGA
TTGACGGCCGCCTCGTCACCAGCGGCGACAAATACGGCACCATCGTCACCGCGATCGACCTCAAGCTCGATCCGACCACC
GGCGACGTGATCAGTGCCGCCGCCAATAACACGATCGTCCGAACCGAAACGCTGGCCAAGGATCCGGCGCAGACCAAACT
GATCGCCGCCTACGACAAACTGGCCGGCCCGATCGCGGCGCGGCCAGCCGGCTCGGTGACCGCGGCGCTGTCGCGGATGC
CGAACGCGGCCGGCGAAAGCGTACTCGGCGACGTCGTCGCCGATGCTCAGCTCGCCGCCACCCTCGACAAGGACAAAGGC
GGCGCTGAGATCGCGCTGACCAATCCGGGCGGCGTCCGCTCCGATATCCTGGGCGGCGAAGGCGGAGCAGTGACATTCGG
CCAAGTGTTTGCCGCGCAGCCGTTCCGCAATCAGCTGGTGACGATGACGCTGACCGGCGCTCAGATCAAGGCGGCGCTCG
AACAGCAATGGGCTGAGCCGGCGCGACCACGCATTCTGCAGGTGTCGAAGGGCTTTCACTTCGCCTGGGATGCTGCGGGC
GCCCCCGGCGGACGAATTCCGCCCGACAGGATGACGCTGAACGGAGCGCCGATCGACCCGAACCGCACCTATCGGGTGAC
GCTCAACGCCTATCTGGCAGCCGGCGGCGACGGCTTCACCGTGTTCAAGGACGGTGCTGCACCGCAAACCGGCGTGTACG
ACGTCGACGCGTTGTTCTCTTATTTCAAGACTCACAGCCCGGTGGTGGCGCCGCCGACCGACCGGGTGACGCGATTGAAT
TAA

Upstream 100 bases:

>100_bases
GGCGGCGTATCGCCGGCACCCTGCGGCAAACCGACGCCACTTGCATTCGCTTGCGCGGCAGGCGATGCGAAGCCTGAAAA
AAGCTCTGCTCGGATCGCCG

Downstream 100 bases:

>100_bases
GCTTGCGGCTGGCTAAGCCTTAAGCGGCCCTTTCGCTCGTCGCTGCGAATAACTACATTCATTCGAATAGATCGCAGACA
CAGATCGGCGCCCGGGCACT

Product: 5'-nucleotidase

Products: ribonucleoside; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MIKLRSRLALLATAAALTVAFVPAPRAAELLPPPVDLRILAINDFHGNLQPPPGGIAIDDPADRTKKIHVPAGGAEHMAT
LVKALRKDHPNAIFVAAGDLIGASPFLSAMFHDEPTIESLSLMGLALSSVGNHEFDEGKTELLRMQNGGCHPVDGCQGPH
PFKGAAFNYLAASTVETATGKTVFPPYAIRKFSGVPVAFIGLTLKNTPNMVSPPGVAGLSFKDEAETVNALIPELKAKGV
EAIVVLIHEGGFPTGDYNECPGISGPIVEIVNKLDRAVDVVISGHTHRAYTCRIDGRLVTSGDKYGTIVTAIDLKLDPTT
GDVISAAANNTIVRTETLAKDPAQTKLIAAYDKLAGPIAARPAGSVTAALSRMPNAAGESVLGDVVADAQLAATLDKDKG
GAEIALTNPGGVRSDILGGEGGAVTFGQVFAAQPFRNQLVTMTLTGAQIKAALEQQWAEPARPRILQVSKGFHFAWDAAG
APGGRIPPDRMTLNGAPIDPNRTYRVTLNAYLAAGGDGFTVFKDGAAPQTGVYDVDALFSYFKTHSPVVAPPTDRVTRLN

Sequences:

>Translated_560_residues
MIKLRSRLALLATAAALTVAFVPAPRAAELLPPPVDLRILAINDFHGNLQPPPGGIAIDDPADRTKKIHVPAGGAEHMAT
LVKALRKDHPNAIFVAAGDLIGASPFLSAMFHDEPTIESLSLMGLALSSVGNHEFDEGKTELLRMQNGGCHPVDGCQGPH
PFKGAAFNYLAASTVETATGKTVFPPYAIRKFSGVPVAFIGLTLKNTPNMVSPPGVAGLSFKDEAETVNALIPELKAKGV
EAIVVLIHEGGFPTGDYNECPGISGPIVEIVNKLDRAVDVVISGHTHRAYTCRIDGRLVTSGDKYGTIVTAIDLKLDPTT
GDVISAAANNTIVRTETLAKDPAQTKLIAAYDKLAGPIAARPAGSVTAALSRMPNAAGESVLGDVVADAQLAATLDKDKG
GAEIALTNPGGVRSDILGGEGGAVTFGQVFAAQPFRNQLVTMTLTGAQIKAALEQQWAEPARPRILQVSKGFHFAWDAAG
APGGRIPPDRMTLNGAPIDPNRTYRVTLNAYLAAGGDGFTVFKDGAAPQTGVYDVDALFSYFKTHSPVVAPPTDRVTRLN
>Mature_560_residues
MIKLRSRLALLATAAALTVAFVPAPRAAELLPPPVDLRILAINDFHGNLQPPPGGIAIDDPADRTKKIHVPAGGAEHMAT
LVKALRKDHPNAIFVAAGDLIGASPFLSAMFHDEPTIESLSLMGLALSSVGNHEFDEGKTELLRMQNGGCHPVDGCQGPH
PFKGAAFNYLAASTVETATGKTVFPPYAIRKFSGVPVAFIGLTLKNTPNMVSPPGVAGLSFKDEAETVNALIPELKAKGV
EAIVVLIHEGGFPTGDYNECPGISGPIVEIVNKLDRAVDVVISGHTHRAYTCRIDGRLVTSGDKYGTIVTAIDLKLDPTT
GDVISAAANNTIVRTETLAKDPAQTKLIAAYDKLAGPIAARPAGSVTAALSRMPNAAGESVLGDVVADAQLAATLDKDKG
GAEIALTNPGGVRSDILGGEGGAVTFGQVFAAQPFRNQLVTMTLTGAQIKAALEQQWAEPARPRILQVSKGFHFAWDAAG
APGGRIPPDRMTLNGAPIDPNRTYRVTLNAYLAAGGDGFTVFKDGAAPQTGVYDVDALFSYFKTHSPVVAPPTDRVTRLN

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=561, Percent_Identity=24.0641711229947, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786687, Length=595, Percent_Identity=22.3529411764706, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790658, Length=567, Percent_Identity=23.6331569664903, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221329836, Length=569, Percent_Identity=25.1318101933216, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19921980, Length=494, Percent_Identity=25.1012145748988, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652512, Length=494, Percent_Identity=25.1012145748988, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161076508, Length=494, Percent_Identity=25.1012145748988, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI28573524, Length=563, Percent_Identity=22.202486678508, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.1.3.5

Molecular weight: Translated: 58548; Mature: 58548

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLRSRLALLATAAALTVAFVPAPRAAELLPPPVDLRILAINDFHGNLQPPPGGIAIDD
CCCHHHHHHHHHHHHHHEEEEECCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCCEEECC
PADRTKKIHVPAGGAEHMATLVKALRKDHPNAIFVAAGDLIGASPFLSAMFHDEPTIESL
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHH
SLMGLALSSVGNHEFDEGKTELLRMQNGGCHPVDGCQGPHPFKGAAFNYLAASTVETATG
HHHHHHHHHCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHEHHCCC
KTVFPPYAIRKFSGVPVAFIGLTLKNTPNMVSPPGVAGLSFKDEAETVNALIPELKAKGV
CEECCCHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
EAIVVLIHEGGFPTGDYNECPGISGPIVEIVNKLDRAVDVVISGHTHRAYTCRIDGRLVT
EEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCEEEE
SGDKYGTIVTAIDLKLDPTTGDVISAAANNTIVRTETLAKDPAQTKLIAAYDKLAGPIAA
CCCCCCEEEEEEEEEECCCCCHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHHCCCCCC
RPAGSVTAALSRMPNAAGESVLGDVVADAQLAATLDKDKGGAEIALTNPGGVRSDILGGE
CCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHEEEECCCCCCCEEEEECCCCCCHHCCCCC
GGAVTFGQVFAAQPFRNQLVTMTLTGAQIKAALEQQWAEPARPRILQVSKGFHFAWDAAG
CCEEEHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCC
APGGRIPPDRMTLNGAPIDPNRTYRVTLNAYLAAGGDGFTVFKDGAAPQTGVYDVDALFS
CCCCCCCCCCEEECCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCCCCCCEEHHHHHH
YFKTHSPVVAPPTDRVTRLN
HHHCCCCEECCCCCCCCCCC
>Mature Secondary Structure
MIKLRSRLALLATAAALTVAFVPAPRAAELLPPPVDLRILAINDFHGNLQPPPGGIAIDD
CCCHHHHHHHHHHHHHHEEEEECCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCCEEECC
PADRTKKIHVPAGGAEHMATLVKALRKDHPNAIFVAAGDLIGASPFLSAMFHDEPTIESL
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHH
SLMGLALSSVGNHEFDEGKTELLRMQNGGCHPVDGCQGPHPFKGAAFNYLAASTVETATG
HHHHHHHHHCCCCCCCCCHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHEHHCCC
KTVFPPYAIRKFSGVPVAFIGLTLKNTPNMVSPPGVAGLSFKDEAETVNALIPELKAKGV
CEECCCHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
EAIVVLIHEGGFPTGDYNECPGISGPIVEIVNKLDRAVDVVISGHTHRAYTCRIDGRLVT
EEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCEEEE
SGDKYGTIVTAIDLKLDPTTGDVISAAANNTIVRTETLAKDPAQTKLIAAYDKLAGPIAA
CCCCCCEEEEEEEEEECCCCCHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHHCCCCCC
RPAGSVTAALSRMPNAAGESVLGDVVADAQLAATLDKDKGGAEIALTNPGGVRSDILGGE
CCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHEEEECCCCCCCEEEEECCCCCCHHCCCCC
GGAVTFGQVFAAQPFRNQLVTMTLTGAQIKAALEQQWAEPARPRILQVSKGFHFAWDAAG
CCEEEHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCC
APGGRIPPDRMTLNGAPIDPNRTYRVTLNAYLAAGGDGFTVFKDGAAPQTGVYDVDALFS
CCCCCCCCCCEEECCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCCCCCCEEHHHHHH
YFKTHSPVVAPPTDRVTRLN
HHHCCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5'-ribonucleotide; H2O

Specific reaction: A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]