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The map label for this gene is acyI [H]

Identifier: 192289272

GI number: 192289272

Start: 892559

End: 894151

Strand: Reverse

Name: acyI [H]

Synonym: Rpal_0844

Alternate gene names: 192289272

Gene position: 894151-892559 (Counterclockwise)

Preceding gene: 192289273

Following gene: 192289270

Centisome position: 15.57

GC content: 70.87

Gene sequence:

>1593_bases
ATGACCGACCAGTTCAGCCATCTGCAGCAGATCCGTAAGCCGGCGATCACCTCCAAGGGCGGCATCGTCGCGGCGCAGTC
GCGCCGGGCGGCCGAGATCGGCGCCGACGTGCTGGCGGAGGGCGGCGACTGCATCGACGCGGTGATCGCCACCACCTTTG
CGCTCGGCGTGCTCGAGCCGTGGATGAGCGGCATCGGCGGCGGCGGCGCGATGGTGCTGTACCGCGCCAAGGAAGACCGC
ACCGAGGTGATCGACTACGGCATGCGGGCGCCGCAGAGCCTGCGGGTCGAGGACTATCCGCTGACCGGCGCCGGCGCAGC
CTCCGATCTGTTTCCGTGGCCGCGGGTCAAGGACGACCGCAACATCCACGGTCCGGCCGCGGTCGCGGTGCCGGGCGTCG
TTGCCGGCATGGAGGAGGCGCACCGCCGCTATGCCAGGCTGCCGTGGCGCGATCTGGTAGCGCCCAGCATCGCGCTCGCC
GGCGACGGGCTTGCGGTCGACTGGTGGACCACGCTGATGATCGGCAGCGTCGCGGCCGATCTGCGTCGGTACCAGGCCAG
CGCCGCGTCGTATCTGATCGACGGGCTGCCGCCGCACGCCCCCTGGGGCATCAAGTCGAACGTCACCCTGCCCCAGGACC
GGCTGCAGGCGACGCTGGCGCAGCTCGGCGAGCAAGGGCCGCGCGACTTCTACGAAGGCGATCTGGCGCGCAGCCTCGCG
GCCGACATCCAGGCCGCCGGCGGCACGCTGTCGGTGGAGGACCTCGCCGGCTTCCGCGCCCATCTGCGCGACCCGCTGAC
GATTCCGTATCGCGGTGGCGCGGTGTTCGCGACGCCGGAGCTGACGGCCGGGCCGACGCTGGCGCACACGCTGCGCGTGC
TCGGCGAGCGCCTGACGCCGGCCGGCGGCGAACCCGACGCCGCGGCCTATCAGGCCTACGCGCAGGCGCTGCAATCGGCC
TATGCGGAGCGGCTGCGCGACATGGGCGACGCCGACGGCCGCCGCGCCCTCGGCGCCGAGCATCTGGCGCCGGCCTGTAC
GACGCATTTCTCGGTGGTCGATCGCGACGGCAACATGGCGGCTGTGACGCAGACTCTGCTGTCGACCTTCGGCTCGAAAT
TCGAAACGCCGCACACCGGCATCATGATGAACAACGGCATCATGTGGTTCGATCCCGAACCCGGCCGGCCGAACTCGCTG
GCGCCGGGCAAGCGCTGCCTGACCAACTACACGCCGGTGCTGGCTCAGGCGCCGGGCGGCCGCCGCTTAGCACTCGGCGC
CTCCGGCGGCCGGCGCATCCTGCCGGCGGTGGCGCAGATCCTGTCGTTCGTGATCGACTACGGCATGGACCTCGACGCCG
CGATCCACCAGCCACGGATCGACGCCAGCGAAGGCGAGGTGGTGATCGGCGACGTGCGCCTGCCTAATGCGACGCGCGAG
GCGCTGCGCCAGAAGTTCGATTACGAAGAGGCGCGGGTGCAGACCTTCCCGATGAAATTCGCCTGCCCCAGCGTGGTGCT
GCGGCACGGAACAACGAATAGCGGCGCGACAGAAATCGCCCAGCCGTGGGGCGACGCCGTGGCAGAAAGCTGA

Upstream 100 bases:

>100_bases
CGTCGTAGCAGCGGCTCGCAGCAGGCCCTACACTGCGCGGCGCCGGCGTAGTCCGCCGGCCCCGCCGCGTGTTGTCCCCT
TTGCCAGCGAAAGCCTCCGC

Downstream 100 bases:

>100_bases
AGCGGCACGAACGTCGCCCGTGCGCAGCAGAGTTAGGCCGGCATCGCCTCGGGTGCCGGCCGAGCACGAACAGTCGTCGT
GCGCTCGGCGCGCGACCTCT

Product: Gamma-glutamyltransferase

Products: NA

Alternate protein names: Cephalosporin acylase; GL-7ACA acylase; Gamma-glutamyltranspeptidase; GGT [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTDQFSHLQQIRKPAITSKGGIVAAQSRRAAEIGADVLAEGGDCIDAVIATTFALGVLEPWMSGIGGGGAMVLYRAKEDR
TEVIDYGMRAPQSLRVEDYPLTGAGAASDLFPWPRVKDDRNIHGPAAVAVPGVVAGMEEAHRRYARLPWRDLVAPSIALA
GDGLAVDWWTTLMIGSVAADLRRYQASAASYLIDGLPPHAPWGIKSNVTLPQDRLQATLAQLGEQGPRDFYEGDLARSLA
ADIQAAGGTLSVEDLAGFRAHLRDPLTIPYRGGAVFATPELTAGPTLAHTLRVLGERLTPAGGEPDAAAYQAYAQALQSA
YAERLRDMGDADGRRALGAEHLAPACTTHFSVVDRDGNMAAVTQTLLSTFGSKFETPHTGIMMNNGIMWFDPEPGRPNSL
APGKRCLTNYTPVLAQAPGGRRLALGASGGRRILPAVAQILSFVIDYGMDLDAAIHQPRIDASEGEVVIGDVRLPNATRE
ALRQKFDYEEARVQTFPMKFACPSVVLRHGTTNSGATEIAQPWGDAVAES

Sequences:

>Translated_530_residues
MTDQFSHLQQIRKPAITSKGGIVAAQSRRAAEIGADVLAEGGDCIDAVIATTFALGVLEPWMSGIGGGGAMVLYRAKEDR
TEVIDYGMRAPQSLRVEDYPLTGAGAASDLFPWPRVKDDRNIHGPAAVAVPGVVAGMEEAHRRYARLPWRDLVAPSIALA
GDGLAVDWWTTLMIGSVAADLRRYQASAASYLIDGLPPHAPWGIKSNVTLPQDRLQATLAQLGEQGPRDFYEGDLARSLA
ADIQAAGGTLSVEDLAGFRAHLRDPLTIPYRGGAVFATPELTAGPTLAHTLRVLGERLTPAGGEPDAAAYQAYAQALQSA
YAERLRDMGDADGRRALGAEHLAPACTTHFSVVDRDGNMAAVTQTLLSTFGSKFETPHTGIMMNNGIMWFDPEPGRPNSL
APGKRCLTNYTPVLAQAPGGRRLALGASGGRRILPAVAQILSFVIDYGMDLDAAIHQPRIDASEGEVVIGDVRLPNATRE
ALRQKFDYEEARVQTFPMKFACPSVVLRHGTTNSGATEIAQPWGDAVAES
>Mature_529_residues
TDQFSHLQQIRKPAITSKGGIVAAQSRRAAEIGADVLAEGGDCIDAVIATTFALGVLEPWMSGIGGGGAMVLYRAKEDRT
EVIDYGMRAPQSLRVEDYPLTGAGAASDLFPWPRVKDDRNIHGPAAVAVPGVVAGMEEAHRRYARLPWRDLVAPSIALAG
DGLAVDWWTTLMIGSVAADLRRYQASAASYLIDGLPPHAPWGIKSNVTLPQDRLQATLAQLGEQGPRDFYEGDLARSLAA
DIQAAGGTLSVEDLAGFRAHLRDPLTIPYRGGAVFATPELTAGPTLAHTLRVLGERLTPAGGEPDAAAYQAYAQALQSAY
AERLRDMGDADGRRALGAEHLAPACTTHFSVVDRDGNMAAVTQTLLSTFGSKFETPHTGIMMNNGIMWFDPEPGRPNSLA
PGKRCLTNYTPVLAQAPGGRRLALGASGGRRILPAVAQILSFVIDYGMDLDAAIHQPRIDASEGEVVIGDVRLPNATREA
LRQKFDYEEARVQTFPMKFACPSVVLRHGTTNSGATEIAQPWGDAVAES

Specific function: Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity [H]

COG id: COG0405

COG function: function code E; Gamma-glutamyltransferase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI73915092, Length=490, Percent_Identity=29.3877551020408, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI73915090, Length=490, Percent_Identity=29.3877551020408, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI73915096, Length=490, Percent_Identity=29.3877551020408, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI73915094, Length=490, Percent_Identity=29.3877551020408, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI113429326, Length=516, Percent_Identity=28.6821705426357, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI113429324, Length=517, Percent_Identity=28.6266924564797, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI153266885, Length=532, Percent_Identity=26.3157894736842, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI153266901, Length=533, Percent_Identity=26.266416510319, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI109148539, Length=494, Percent_Identity=28.7449392712551, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI153266907, Length=525, Percent_Identity=25.3333333333333, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI30581157, Length=124, Percent_Identity=32.258064516129, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI30581155, Length=124, Percent_Identity=32.258064516129, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789856, Length=497, Percent_Identity=31.3883299798793, Blast_Score=212, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI212646519, Length=493, Percent_Identity=26.1663286004057, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17506189, Length=492, Percent_Identity=26.219512195122, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI72000294, Length=493, Percent_Identity=26.1663286004057, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17542894, Length=490, Percent_Identity=25.5102040816327, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI193208247, Length=543, Percent_Identity=24.4935543278085, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI193208245, Length=543, Percent_Identity=24.4935543278085, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI72001497, Length=314, Percent_Identity=22.2929936305732, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI72001495, Length=314, Percent_Identity=22.2929936305732, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323330, Length=487, Percent_Identity=23.8193018480493, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI161077880, Length=485, Percent_Identity=27.6288659793814, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24642670, Length=485, Percent_Identity=27.6288659793814, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24642666, Length=485, Percent_Identity=27.6288659793814, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI45551474, Length=485, Percent_Identity=27.6288659793814, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI161077878, Length=485, Percent_Identity=27.6288659793814, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI18859893, Length=495, Percent_Identity=27.2727272727273, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI161077496, Length=474, Percent_Identity=28.2700421940928, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI18859741, Length=488, Percent_Identity=25.4098360655738, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000101 [H]

Pfam domain/function: PF01019 G_glu_transpept [H]

EC number: =2.3.2.2 [H]

Molecular weight: Translated: 56494; Mature: 56362

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQFSHLQQIRKPAITSKGGIVAAQSRRAAEIGADVLAEGGDCIDAVIATTFALGVLEP
CCCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
WMSGIGGGGAMVLYRAKEDRTEVIDYGMRAPQSLRVEDYPLTGAGAASDLFPWPRVKDDR
HHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NIHGPAAVAVPGVVAGMEEAHRRYARLPWRDLVAPSIALAGDGLAVDWWTTLMIGSVAAD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEECCCCEEHHHHHHHHHHHHHHH
LRRYQASAASYLIDGLPPHAPWGIKSNVTLPQDRLQATLAQLGEQGPRDFYEGDLARSLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ADIQAAGGTLSVEDLAGFRAHLRDPLTIPYRGGAVFATPELTAGPTLAHTLRVLGERLTP
HHHHHCCCEEEHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHHHCCC
AGGEPDAAAYQAYAQALQSAYAERLRDMGDADGRRALGAEHLAPACTTHFSVVDRDGNMA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHCHHHHHHEEEEECCCCHH
AVTQTLLSTFGSKFETPHTGIMMNNGIMWFDPEPGRPNSLAPGKRCLTNYTPVLAQAPGG
HHHHHHHHHHCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCC
RRLALGASGGRRILPAVAQILSFVIDYGMDLDAAIHQPRIDASEGEVVIGDVRLPNATRE
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEEECCHHHHH
ALRQKFDYEEARVQTFPMKFACPSVVLRHGTTNSGATEIAQPWGDAVAES
HHHHHCCHHHHHHEECCHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDQFSHLQQIRKPAITSKGGIVAAQSRRAAEIGADVLAEGGDCIDAVIATTFALGVLEP
CCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
WMSGIGGGGAMVLYRAKEDRTEVIDYGMRAPQSLRVEDYPLTGAGAASDLFPWPRVKDDR
HHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NIHGPAAVAVPGVVAGMEEAHRRYARLPWRDLVAPSIALAGDGLAVDWWTTLMIGSVAAD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEECCCCEEHHHHHHHHHHHHHHH
LRRYQASAASYLIDGLPPHAPWGIKSNVTLPQDRLQATLAQLGEQGPRDFYEGDLARSLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ADIQAAGGTLSVEDLAGFRAHLRDPLTIPYRGGAVFATPELTAGPTLAHTLRVLGERLTP
HHHHHCCCEEEHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHHHCCC
AGGEPDAAAYQAYAQALQSAYAERLRDMGDADGRRALGAEHLAPACTTHFSVVDRDGNMA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHCHHHHHHEEEEECCCCHH
AVTQTLLSTFGSKFETPHTGIMMNNGIMWFDPEPGRPNSLAPGKRCLTNYTPVLAQAPGG
HHHHHHHHHHCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCC
RRLALGASGGRRILPAVAQILSFVIDYGMDLDAAIHQPRIDASEGEVVIGDVRLPNATRE
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEEECCHHHHH
ALRQKFDYEEARVQTFPMKFACPSVVLRHGTTNSGATEIAQPWGDAVAES
HHHHHCCHHHHHHEECCHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3680178 [H]