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The map label for this gene is phnJ [H]

Identifier: 192289188

GI number: 192289188

Start: 801157

End: 802035

Strand: Reverse

Name: phnJ [H]

Synonym: Rpal_0759

Alternate gene names: 192289188

Gene position: 802035-801157 (Counterclockwise)

Preceding gene: 192289189

Following gene: 192289187

Centisome position: 13.96

GC content: 64.05

Gene sequence:

>879_bases
ATGACCGAGGCGACCTACAATTTCGCTTATCTCGACGAGCAGACCAAGCGGATGATCCGCCGCGCGATCCTGAAAGGAAT
CGCGATCCCCGGCTATCAGGTGCCGTTCGCCAGCCGCGAGATGCCGATGCCTTATGGCTGGGGCACCGGCGGTGTACAGG
TGACGGCGTCGATCCTCGGTCCCGACGATACGCTGAAGGTGATCGACCAGGGCAGCGACGACACCACCAACGCGATCTCG
ATCCGCAAGTTCTTCGCCAAGACCGCCTGCGTGGCCACCACCACGGCGACGCCGGACGCGAGCGTGATCCAGACCCGGCA
CCGCATTCCCGAAACGCCGCTGCACGAAGGCCAGGTGCTGGTGTATCAGGTGCCGATCCCCGAACCGCTGCGCTATCTCG
AACCGCGCGAGACCGAGACGCGGCGCATGCATGCGCTCGCTGAATACGGCCTGATGCACGTCAAGCTGTACGAGGACATC
GCCAAGTTCGGCCACATCGCCACCGCCTATGCCTATCCGGTGAAGGTCGCGGGCCGCTACGTGATGGACCCGTCACCGAC
GCCGAAGTTCGACAACCCGAAGATGGACAACTGCCCGGCGCTGCAACTGTTCGGCGCCGGCCGCGAGAAGCGGATCTACG
CGATCCCGCCTTACACGCAGGTGGTGTCGCTGGATTTCGAGGACTATCCGTTCACGCGCTATCGCCAGGACGGCTGCTGT
GCGCTGTGCGGTGCCGACGACTCGTATCTCGACGAGATCGTCACCGATAACGCCGGCACCCGGATGTTCGTGTGCTCGGA
CACAGATTATTGCGAAGGCCGCCGCGCCCAGGGTCACACCGGCAGCGAAACGGCCCCGATGGAGAGCACCCATGCTTGA

Upstream 100 bases:

>100_bases
AGCTGCCGCACTACGTCGATTTCCAGTCCGAGCTCGGCCTGCTGCGCAAGCTGCGCGCCGAATTCAACCAGGACCAACCC
GAGCTGCGCGAGGCTGCGGA

Downstream 100 bases:

>100_bases
TCGCGTCTCATCCGCCGCATCAGGCGCCTTCACCGCGGCCGACCAGCCGCTGCTGATCGCCGACGGCCTGTCGAAACGCT
ACGGCCGGATCGACGCCTGT

Product: phosphonate metabolism PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTEATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASILGPDDTLKVIDQGSDDTTNAIS
IRKFFAKTACVATTTATPDASVIQTRHRIPETPLHEGQVLVYQVPIPEPLRYLEPRETETRRMHALAEYGLMHVKLYEDI
AKFGHIATAYAYPVKVAGRYVMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTQVVSLDFEDYPFTRYRQDGCC
ALCGADDSYLDEIVTDNAGTRMFVCSDTDYCEGRRAQGHTGSETAPMESTHA

Sequences:

>Translated_292_residues
MTEATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASILGPDDTLKVIDQGSDDTTNAIS
IRKFFAKTACVATTTATPDASVIQTRHRIPETPLHEGQVLVYQVPIPEPLRYLEPRETETRRMHALAEYGLMHVKLYEDI
AKFGHIATAYAYPVKVAGRYVMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTQVVSLDFEDYPFTRYRQDGCC
ALCGADDSYLDEIVTDNAGTRMFVCSDTDYCEGRRAQGHTGSETAPMESTHA
>Mature_291_residues
TEATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASILGPDDTLKVIDQGSDDTTNAISI
RKFFAKTACVATTTATPDASVIQTRHRIPETPLHEGQVLVYQVPIPEPLRYLEPRETETRRMHALAEYGLMHVKLYEDIA
KFGHIATAYAYPVKVAGRYVMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTQVVSLDFEDYPFTRYRQDGCCA
LCGADDSYLDEIVTDNAGTRMFVCSDTDYCEGRRAQGHTGSETAPMESTHA

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=266, Percent_Identity=76.6917293233083, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 32560; Mature: 32429

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASILG
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
PDDTLKVIDQGSDDTTNAISIRKFFAKTACVATTTATPDASVIQTRHRIPETPLHEGQVL
CCCHHHEEECCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCCEEE
VYQVPIPEPLRYLEPRETETRRMHALAEYGLMHVKLYEDIAKFGHIATAYAYPVKVAGRY
EEECCCCCHHHHCCCCCHHHHHHHHHHHHCEEEEHHHHHHHHHCCHHHHHCCEEEECCEE
VMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTQVVSLDFEDYPFTRYRQDGCC
EECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCEEEEEECCCCCCCHHCCCCCCE
ALCGADDSYLDEIVTDNAGTRMFVCSDTDYCEGRRAQGHTGSETAPMESTHA
EEECCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEATYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASILG
CCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
PDDTLKVIDQGSDDTTNAISIRKFFAKTACVATTTATPDASVIQTRHRIPETPLHEGQVL
CCCHHHEEECCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCCEEE
VYQVPIPEPLRYLEPRETETRRMHALAEYGLMHVKLYEDIAKFGHIATAYAYPVKVAGRY
EEECCCCCHHHHCCCCCHHHHHHHHHHHHCEEEEHHHHHHHHHCCHHHHHCCEEEECCEE
VMDPSPTPKFDNPKMDNCPALQLFGAGREKRIYAIPPYTQVVSLDFEDYPFTRYRQDGCC
EECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCEEEEEECCCCCCCHHCCCCCCE
ALCGADDSYLDEIVTDNAGTRMFVCSDTDYCEGRRAQGHTGSETAPMESTHA
EEECCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]