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The map label for this gene is phnI [H]

Identifier: 192289189

GI number: 192289189

Start: 802032

End: 803129

Strand: Reverse

Name: phnI [H]

Synonym: Rpal_0760

Alternate gene names: 192289189

Gene position: 803129-802032 (Counterclockwise)

Preceding gene: 192289190

Following gene: 192289188

Centisome position: 13.98

GC content: 66.67

Gene sequence:

>1098_bases
ATGTATGTCGCAGTCAAAGGCGGCGAACGCGCCATCGACAATGCCCACCGGTTGCTCGCCGATGCGCGACGTGGGAAACG
CGACGTGCCGGAGCTGACGCTCGACCAGATCTCCGAACAGCTCGCACTCGGCGTCGATCGGGTGATGAGCGAAGGCTCGC
TGTACGACCGCGAGCTTGCAGCCCTGGCGATCAAGCAGGCGCGCGGCGATCTGATCGAAGCGATCTTCCTGGTGCGGGCG
TTCCGCGCCACGCTGCCGCGGTTCGGCTCGTCCGAGCCCGTCGACACCGGCGCAATGGCCGTGCGTCGGCGCATTTCCTC
GACTTTCAAGGACATTCCGGGCGGCCAGATCCTCGGCCCCACCTTCGACTACACCCATCGGCTGCTCAATCCGCAGCTCG
CCGAAGGCGGAGATCCCGCTGAGCCGGCGACAGCGGAAGCCAGTGACGCGCCGACGCCGCGGGTGACCGACATCCTGCGC
CAGGACGGGCTGATCGAAGAATCGCCGGTTGCGGATGACGACAGCGCAGTCGGCGACCTCACCCGCGAGCCGCTGAACTT
TCCGGCCGGGCGCGACCTTCGGCTGCAGAACCTCGCCCGTGCCGACGAGGGCTTTCTGCTGGCGCTCGGCTATTCCACTC
AGCGCGGCTACGGCCGCAATCATCCATTCGCCGGCGAAATCCGGTTCGGCGAAGTCGAGGTGTTCTTCGAGGCCGAAGAC
GCCGGCTTCGCGGTGCCGCTCGGCAGCGTGGCGCTGACCGAGTGCCAGATGGTCAATCAGTTCAAAGGCTCGGCAACCGA
GGCGCCGCGATTCACCCGAGGCTATGGCCTCGCCTTCGGCCAGAGCGAGCGCAAGGCGATGTCGATAGCCCTGGTCGATC
GCGCGCTGCGCGCGCAAGAACTCGGCGAGGACGTCAAGGCGCCGGCGCAGGACGAGGAGTTCGTGCTGTCGCATTCGGAC
AACGTCCAGGCCACCGGCTTCGTCGAGCATCTGAAGCTGCCGCACTACGTCGATTTCCAGTCCGAGCTCGGCCTGCTGCG
CAAGCTGCGCGCCGAATTCAACCAGGACCAACCCGAGCTGCGCGAGGCTGCGGAATGA

Upstream 100 bases:

>100_bases
TGCGGCGCTGTTTCCGCGCGGCGTCGACGTGGTGCTGGTGAACGGCGATCAGGTGGTGGCGATCCCGCGCACCACGCGCC
TCAGCCAAGGGAGCTGACCG

Downstream 100 bases:

>100_bases
CCGAGGCGACCTACAATTTCGCTTATCTCGACGAGCAGACCAAGCGGATGATCCGCCGCGCGATCCTGAAAGGAATCGCG
ATCCCCGGCTATCAGGTGCC

Product: phosphonate metabolism protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MYVAVKGGERAIDNAHRLLADARRGKRDVPELTLDQISEQLALGVDRVMSEGSLYDRELAALAIKQARGDLIEAIFLVRA
FRATLPRFGSSEPVDTGAMAVRRRISSTFKDIPGGQILGPTFDYTHRLLNPQLAEGGDPAEPATAEASDAPTPRVTDILR
QDGLIEESPVADDDSAVGDLTREPLNFPAGRDLRLQNLARADEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVFFEAED
AGFAVPLGSVALTECQMVNQFKGSATEAPRFTRGYGLAFGQSERKAMSIALVDRALRAQELGEDVKAPAQDEEFVLSHSD
NVQATGFVEHLKLPHYVDFQSELGLLRKLRAEFNQDQPELREAAE

Sequences:

>Translated_365_residues
MYVAVKGGERAIDNAHRLLADARRGKRDVPELTLDQISEQLALGVDRVMSEGSLYDRELAALAIKQARGDLIEAIFLVRA
FRATLPRFGSSEPVDTGAMAVRRRISSTFKDIPGGQILGPTFDYTHRLLNPQLAEGGDPAEPATAEASDAPTPRVTDILR
QDGLIEESPVADDDSAVGDLTREPLNFPAGRDLRLQNLARADEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVFFEAED
AGFAVPLGSVALTECQMVNQFKGSATEAPRFTRGYGLAFGQSERKAMSIALVDRALRAQELGEDVKAPAQDEEFVLSHSD
NVQATGFVEHLKLPHYVDFQSELGLLRKLRAEFNQDQPELREAAE
>Mature_365_residues
MYVAVKGGERAIDNAHRLLADARRGKRDVPELTLDQISEQLALGVDRVMSEGSLYDRELAALAIKQARGDLIEAIFLVRA
FRATLPRFGSSEPVDTGAMAVRRRISSTFKDIPGGQILGPTFDYTHRLLNPQLAEGGDPAEPATAEASDAPTPRVTDILR
QDGLIEESPVADDDSAVGDLTREPLNFPAGRDLRLQNLARADEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVFFEAED
AGFAVPLGSVALTECQMVNQFKGSATEAPRFTRGYGLAFGQSERKAMSIALVDRALRAQELGEDVKAPAQDEEFVLSHSD
NVQATGFVEHLKLPHYVDFQSELGLLRKLRAEFNQDQPELREAAE

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=357, Percent_Identity=59.6638655462185, Blast_Score=419, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 39982; Mature: 39982

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGERAIDNAHRLLADARRGKRDVPELTLDQISEQLALGVDRVMSEGSLYDRELA
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ALAIKQARGDLIEAIFLVRAFRATLPRFGSSEPVDTGAMAVRRRISSTFKDIPGGQILGP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECC
TFDYTHRLLNPQLAEGGDPAEPATAEASDAPTPRVTDILRQDGLIEESPVADDDSAVGDL
CHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHH
TREPLNFPAGRDLRLQNLARADEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVFFEAED
HHCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECEEEEEEEECC
AGFAVPLGSVALTECQMVNQFKGSATEAPRFTRGYGLAFGQSERKAMSIALVDRALRAQE
CCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHH
LGEDVKAPAQDEEFVLSHSDNVQATGFVEHLKLPHYVDFQSELGLLRKLRAEFNQDQPEL
HHHHHCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHH
REAAE
HHHCC
>Mature Secondary Structure
MYVAVKGGERAIDNAHRLLADARRGKRDVPELTLDQISEQLALGVDRVMSEGSLYDRELA
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ALAIKQARGDLIEAIFLVRAFRATLPRFGSSEPVDTGAMAVRRRISSTFKDIPGGQILGP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECC
TFDYTHRLLNPQLAEGGDPAEPATAEASDAPTPRVTDILRQDGLIEESPVADDDSAVGDL
CHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHH
TREPLNFPAGRDLRLQNLARADEGFLLALGYSTQRGYGRNHPFAGEIRFGEVEVFFEAED
HHCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECEEEEEEEECC
AGFAVPLGSVALTECQMVNQFKGSATEAPRFTRGYGLAFGQSERKAMSIALVDRALRAQE
CCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHH
LGEDVKAPAQDEEFVLSHSDNVQATGFVEHLKLPHYVDFQSELGLLRKLRAEFNQDQPEL
HHHHHCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHH
REAAE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]