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The map label for this gene is emrA [H]

Identifier: 192288946

GI number: 192288946

Start: 564272

End: 565441

Strand: Reverse

Name: emrA [H]

Synonym: Rpal_0516

Alternate gene names: 192288946

Gene position: 565441-564272 (Counterclockwise)

Preceding gene: 192288947

Following gene: 192288945

Centisome position: 9.84

GC content: 67.09

Gene sequence:

>1170_bases
ATGGCTGAACCGGCGCTAAAATTTCCGACCGAGCAGCAGGAGCCTGCGGCGGATCCGGCCGTCGCCACGAGTGGCGGACC
GCGCCGCGGTATCGGCGGCACGCTGCGTCGCTATCGGCGGCCGCTGCTGCTGATCGTTCTGCCGCTGGTGGCACTGATCG
GCGGCGTCACCTTCTATCTGGAGGGCGGCCGCTACGTTACCACCGACGACGCTTATGTCGGCGCCCAGAAGGTGCTGATC
ACGCCGGACGTCGCCGGCAAGATCGTCGAAGTGACCGTCAAGGAAGGCCAACGCGTCCAACCCGGTGACGAGCTGTTCCA
GATCGATCCGGTGCCGTTCCGCCTCGCGGTGGCGCAGGCCCAGGCCAAGCTCGCCGACGCCAAGACCAGCCACGCCAATC
TGGTCGCCAACGTCAAGCTGTACGGCCAGACCATCGACCTGGTGAACGCCGGCATCGCGCTGAAGCAGCGCGACGTCGAG
CGCAAGACGTCGCTGGTGCAGAGCCGCGCCGGTTCGCAGCTCGACCTCGACAACTCCACCGCCGCGCTGGTGACCGCGCA
AGCGCAGCTGCAGCTCGTCAAGCAGCAGCAGTCGACCGCGCTCAATCAGTTGCTCGGCGATCCCGAACTGCCGCTGGAGA
AATTCCCCGCCTATGCCCAGGCCAAGGCGGCGCTCGACGACGCCGAACGTAACCTGCGGCTGTCCACCGTGCGGGCGCCG
ATGAGCGGCACCGCCACCCAGGTCGACAACATCCAGCTCGGCCGCTTCGTCGCCGCCGGCACGCCGGTGTTCAGCGTGAT
CGACACCACGCAGCCATGGGTCGACGCCAATCCTAAGGAAAGCGACTTCACCTATGTGGCGGTGGGCCAGACCGTGACGC
TCGATGTCGACGCCTTCCCGGATCATCAGTTCAAGGGCCGGGTGTCGTCGCTGTCGCCGGGCACTGGCGCGCAATTCGCC
GTGCTGCCGCCGCAGAACGCTACCGGCAACTTCGTCAAGGTAGTGCAGCGCGTGCCGCTGCGGATCGCGCTCGACGAGAC
CGATCCGATGATCAAGCGGCTGAAGGCCGGCATGAGCGTCAACGTCGCGATCGACACCCACCACCGCCGTTCGTTGGCCG
GCCTGCTCGGCTTCGGCCACGCCGCAGCTGCAACGCCGGAGCACAACTAA

Upstream 100 bases:

>100_bases
GCCCAGCGCCTGGTGCGGCAGATGCAGATCATCAAAGAAAATTTGCGGGGCGCGATCCAACACCACTGCCACCGCGAAGA
AAAACGACGGGAGGAGCACT

Downstream 100 bases:

>100_bases
CCGATGGCGGGCGCGGCACAATCTCACGCGGTCCCCGGCTTTCGCCGGAACATGGTGACGATCTGCGCCATGACCGCCAC
GATCATGCAGGCGCTCGACA

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MAEPALKFPTEQQEPAADPAVATSGGPRRGIGGTLRRYRRPLLLIVLPLVALIGGVTFYLEGGRYVTTDDAYVGAQKVLI
TPDVAGKIVEVTVKEGQRVQPGDELFQIDPVPFRLAVAQAQAKLADAKTSHANLVANVKLYGQTIDLVNAGIALKQRDVE
RKTSLVQSRAGSQLDLDNSTAALVTAQAQLQLVKQQQSTALNQLLGDPELPLEKFPAYAQAKAALDDAERNLRLSTVRAP
MSGTATQVDNIQLGRFVAAGTPVFSVIDTTQPWVDANPKESDFTYVAVGQTVTLDVDAFPDHQFKGRVSSLSPGTGAQFA
VLPPQNATGNFVKVVQRVPLRIALDETDPMIKRLKAGMSVNVAIDTHHRRSLAGLLGFGHAAAATPEHN

Sequences:

>Translated_389_residues
MAEPALKFPTEQQEPAADPAVATSGGPRRGIGGTLRRYRRPLLLIVLPLVALIGGVTFYLEGGRYVTTDDAYVGAQKVLI
TPDVAGKIVEVTVKEGQRVQPGDELFQIDPVPFRLAVAQAQAKLADAKTSHANLVANVKLYGQTIDLVNAGIALKQRDVE
RKTSLVQSRAGSQLDLDNSTAALVTAQAQLQLVKQQQSTALNQLLGDPELPLEKFPAYAQAKAALDDAERNLRLSTVRAP
MSGTATQVDNIQLGRFVAAGTPVFSVIDTTQPWVDANPKESDFTYVAVGQTVTLDVDAFPDHQFKGRVSSLSPGTGAQFA
VLPPQNATGNFVKVVQRVPLRIALDETDPMIKRLKAGMSVNVAIDTHHRRSLAGLLGFGHAAAATPEHN
>Mature_388_residues
AEPALKFPTEQQEPAADPAVATSGGPRRGIGGTLRRYRRPLLLIVLPLVALIGGVTFYLEGGRYVTTDDAYVGAQKVLIT
PDVAGKIVEVTVKEGQRVQPGDELFQIDPVPFRLAVAQAQAKLADAKTSHANLVANVKLYGQTIDLVNAGIALKQRDVER
KTSLVQSRAGSQLDLDNSTAALVTAQAQLQLVKQQQSTALNQLLGDPELPLEKFPAYAQAKAALDDAERNLRLSTVRAPM
SGTATQVDNIQLGRFVAAGTPVFSVIDTTQPWVDANPKESDFTYVAVGQTVTLDVDAFPDHQFKGRVSSLSPGTGAQFAV
LPPQNATGNFVKVVQRVPLRIALDETDPMIKRLKAGMSVNVAIDTHHRRSLAGLLGFGHAAAATPEHN

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=322, Percent_Identity=32.6086956521739, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788711, Length=342, Percent_Identity=30.7017543859649, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1790519, Length=310, Percent_Identity=31.9354838709677, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081951, Length=327, Percent_Identity=25.6880733944954, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789637, Length=364, Percent_Identity=24.7252747252747, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41587; Mature: 41456

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPALKFPTEQQEPAADPAVATSGGPRRGIGGTLRRYRRPLLLIVLPLVALIGGVTFYL
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCEEEEE
EGGRYVTTDDAYVGAQKVLITPDVAGKIVEVTVKEGQRVQPGDELFQIDPVPFRLAVAQA
ECCEEEECCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEHHHHH
QAKLADAKTSHANLVANVKLYGQTIDLVNAGIALKQRDVERKTSLVQSRAGSQLDLDNST
HHHHHHCCCCCCEEEEEEEEECCEEEHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCC
AALVTAQAQLQLVKQQQSTALNQLLGDPELPLEKFPAYAQAKAALDDAERNLRLSTVRAP
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCEEEEEEECC
MSGTATQVDNIQLGRFVAAGTPVFSVIDTTQPWVDANPKESDFTYVAVGQTVTLDVDAFP
CCCCCEECCCEEECEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEECCEEEEEEECCC
DHQFKGRVSSLSPGTGAQFAVLPPQNATGNFVKVVQRVPLRIALDETDPMIKRLKAGMSV
CCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHCCCEE
NVAIDTHHRRSLAGLLGFGHAAAATPEHN
EEEEECCHHHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure 
AEPALKFPTEQQEPAADPAVATSGGPRRGIGGTLRRYRRPLLLIVLPLVALIGGVTFYL
CCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCEEEEE
EGGRYVTTDDAYVGAQKVLITPDVAGKIVEVTVKEGQRVQPGDELFQIDPVPFRLAVAQA
ECCEEEECCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEHHHHH
QAKLADAKTSHANLVANVKLYGQTIDLVNAGIALKQRDVERKTSLVQSRAGSQLDLDNST
HHHHHHCCCCCCEEEEEEEEECCEEEHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCC
AALVTAQAQLQLVKQQQSTALNQLLGDPELPLEKFPAYAQAKAALDDAERNLRLSTVRAP
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCEEEEEEECC
MSGTATQVDNIQLGRFVAAGTPVFSVIDTTQPWVDANPKESDFTYVAVGQTVTLDVDAFP
CCCCCEECCCEEECEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEECCEEEEEEECCC
DHQFKGRVSSLSPGTGAQFAVLPPQNATGNFVKVVQRVPLRIALDETDPMIKRLKAGMSV
CCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHCCCEE
NVAIDTHHRRSLAGLLGFGHAAAATPEHN
EEEEECCHHHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]