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The map label for this gene is emrB [H]

Identifier: 192288945

GI number: 192288945

Start: 562706

End: 564268

Strand: Reverse

Name: emrB [H]

Synonym: Rpal_0515

Alternate gene names: 192288945

Gene position: 564268-562706 (Counterclockwise)

Preceding gene: 192288946

Following gene: 192288943

Centisome position: 9.82

GC content: 63.66

Gene sequence:

>1563_bases
ATGGCGGGCGCGGCACAATCTCACGCGGTCCCCGGCTTTCGCCGGAACATGGTGACGATCTGCGCCATGACCGCCACGAT
CATGCAGGCGCTCGACACCACGATCGCCAACGTCGCGTTGCCCTATATGCAGGGCTCGCTGTCGGCGTCTCAGGACCAGA
TCAACTGGGTGCTGACCTCCTACATCGTCGCCGCCGCGATCATGACGGCGCCGGTGGGCTGGATCGCCAACCGGTTCGGC
CGCAAGCGCATCTTCATCATCTGCTCGGCCGGATTCACCATCGCGTCGGTGATGTGCGGCCTTGCCCAGGATATCACCCA
GATGGTGGTGTTCCGGCTGCTACAGGGCGTGTTCGGCGCCGCGCTGGTGCCGCTGTCGCAGGCGGTGATGCTCGACAGCT
ACGCGCTGCACGAGCGCGCCAAGGCGATGTCGATCTGGGGCATGGGCGTGATGATGGGGCCGATCATGGGCCCCTCGCTC
GGCGCCTGGCTGACTGAAACGTACTCCTGGCACTGGGTGTTCTTCGTCAACCTGCCGTTCGGCGCCATCACGGTGCTGGG
TCTGGCGATGTTCATGGACGAAACCGATAAGAACCAGGACTTGAAGTTCGACTGGTTCGGCTTCGCGGCGCTCGCGGTCG
GCATCGGCGCGATGCAGCTCGCGCTCGACCGCGGCGAACAGCTCGATTGGTTCAACTCGCCCGAGATCATCATCGAGTCG
ATCGTCTCGGTGGTCGGGTTCTACTACTTCTTCGCCCACTCCTTCACCACCGAGCGCCCCTTCATCCAGTTCGCGATCTT
CAAGGACCGCAACTTCGTCGGCGGCGTGGTGTTCATGACGGTGATGGGGCTGGTTCTGTTCTCCACCATGGCGCTGTCGT
CGCCGTTCCTGCAGAACGTCGAGGGCTATCCGATCATGACCGCCGGCCTGCTGCTGGCTTCGCGCGGTTGCGGTACCTTC
GTGGCGATGATGATCGTCGGGCGGATCATGCGCTATATCGAGGCCCGCACCCTGATCGCCACTGGGCTGTCGCTGACCTG
CGCGTCGCTCTATGTGATGACCGGCTGGACCGATCAGACCAGCGTGTCGAGCGTGGTGGTGACCAGCATCATCCAGGGCT
TCGGCTTCGGTCTGGTATTCGTGCCGCTGTCGACGGTGGCGTTCATGACGCTGCCCGGACACCTGCGCACCGACGGCACC
GCGATGCTGACGCTGGTGCGCAACGTCGCCAGTTCGGTCGGGATCTCAGTGGTGATCGCCAAACTGTCGTCGGGCACCAC
CACGGCGCACGCCATCCTGTCCGAGCACATCAACCCGTTCAACAACGCGCTGCAGATGCCCAACGTCACCGGCATGATCG
ATCTGTCGACCACCACCGGCAAGGCGATGATGGACGCGATCGTCACCATGCAGGCGCAGATCATCGCATTCTCGCACGAC
TACGCGATGGTGATGTTCCTGACCGCCGCGGCGGTGCCGATGGCGATCATCATCGGCTCGACTAAGGCGGCGCTACGCAA
GCAAAGCGAAGCCCCGGCGGATCATGCCGCGGTGATGGAGTAG

Upstream 100 bases:

>100_bases
GAGCGTCAACGTCGCGATCGACACCCACCACCGCCGTTCGTTGGCCGGCCTGCTCGGCTTCGGCCACGCCGCAGCTGCAA
CGCCGGAGCACAACTAACCG

Downstream 100 bases:

>100_bases
CCTGCTTACCTCTCTCCGCAAGAGCGGGGCGAGGGAGTTCACCGGAGGCGCCGCCTCAAATCGCGGCGTCATGCGCGGGC
TTGCCCCGCGCATCCATCGC

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MAGAAQSHAVPGFRRNMVTICAMTATIMQALDTTIANVALPYMQGSLSASQDQINWVLTSYIVAAAIMTAPVGWIANRFG
RKRIFIICSAGFTIASVMCGLAQDITQMVVFRLLQGVFGAALVPLSQAVMLDSYALHERAKAMSIWGMGVMMGPIMGPSL
GAWLTETYSWHWVFFVNLPFGAITVLGLAMFMDETDKNQDLKFDWFGFAALAVGIGAMQLALDRGEQLDWFNSPEIIIES
IVSVVGFYYFFAHSFTTERPFIQFAIFKDRNFVGGVVFMTVMGLVLFSTMALSSPFLQNVEGYPIMTAGLLLASRGCGTF
VAMMIVGRIMRYIEARTLIATGLSLTCASLYVMTGWTDQTSVSSVVVTSIIQGFGFGLVFVPLSTVAFMTLPGHLRTDGT
AMLTLVRNVASSVGISVVIAKLSSGTTTAHAILSEHINPFNNALQMPNVTGMIDLSTTTGKAMMDAIVTMQAQIIAFSHD
YAMVMFLTAAAVPMAIIIGSTKAALRKQSEAPADHAAVME

Sequences:

>Translated_520_residues
MAGAAQSHAVPGFRRNMVTICAMTATIMQALDTTIANVALPYMQGSLSASQDQINWVLTSYIVAAAIMTAPVGWIANRFG
RKRIFIICSAGFTIASVMCGLAQDITQMVVFRLLQGVFGAALVPLSQAVMLDSYALHERAKAMSIWGMGVMMGPIMGPSL
GAWLTETYSWHWVFFVNLPFGAITVLGLAMFMDETDKNQDLKFDWFGFAALAVGIGAMQLALDRGEQLDWFNSPEIIIES
IVSVVGFYYFFAHSFTTERPFIQFAIFKDRNFVGGVVFMTVMGLVLFSTMALSSPFLQNVEGYPIMTAGLLLASRGCGTF
VAMMIVGRIMRYIEARTLIATGLSLTCASLYVMTGWTDQTSVSSVVVTSIIQGFGFGLVFVPLSTVAFMTLPGHLRTDGT
AMLTLVRNVASSVGISVVIAKLSSGTTTAHAILSEHINPFNNALQMPNVTGMIDLSTTTGKAMMDAIVTMQAQIIAFSHD
YAMVMFLTAAAVPMAIIIGSTKAALRKQSEAPADHAAVME
>Mature_519_residues
AGAAQSHAVPGFRRNMVTICAMTATIMQALDTTIANVALPYMQGSLSASQDQINWVLTSYIVAAAIMTAPVGWIANRFGR
KRIFIICSAGFTIASVMCGLAQDITQMVVFRLLQGVFGAALVPLSQAVMLDSYALHERAKAMSIWGMGVMMGPIMGPSLG
AWLTETYSWHWVFFVNLPFGAITVLGLAMFMDETDKNQDLKFDWFGFAALAVGIGAMQLALDRGEQLDWFNSPEIIIESI
VSVVGFYYFFAHSFTTERPFIQFAIFKDRNFVGGVVFMTVMGLVLFSTMALSSPFLQNVEGYPIMTAGLLLASRGCGTFV
AMMIVGRIMRYIEARTLIATGLSLTCASLYVMTGWTDQTSVSSVVVTSIIQGFGFGLVFVPLSTVAFMTLPGHLRTDGTA
MLTLVRNVASSVGISVVIAKLSSGTTTAHAILSEHINPFNNALQMPNVTGMIDLSTTTGKAMMDAIVTMQAQIIAFSHDY
AMVMFLTAAAVPMAIIIGSTKAALRKQSEAPADHAAVME

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=485, Percent_Identity=32.1649484536082, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788710, Length=430, Percent_Identity=33.953488372093, Blast_Score=245, Evalue=6e-66,
Organism=Escherichia coli, GI1790195, Length=429, Percent_Identity=29.1375291375291, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788392, Length=396, Percent_Identity=30.3030303030303, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=25.1196172248804, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87082320, Length=175, Percent_Identity=25.1428571428571, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790146, Length=180, Percent_Identity=29.4444444444444, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=239, Percent_Identity=22.1757322175732, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323000, Length=132, Percent_Identity=25, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=157, Percent_Identity=25.4777070063694, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56032; Mature: 55901

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.9 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
6.7 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAAQSHAVPGFRRNMVTICAMTATIMQALDTTIANVALPYMQGSLSASQDQINWVLTS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
YIVAAAIMTAPVGWIANRFGRKRIFIICSAGFTIASVMCGLAQDITQMVVFRLLQGVFGA
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVPLSQAVMLDSYALHERAKAMSIWGMGVMMGPIMGPSLGAWLTETYSWHWVFFVNLPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEEEEECCH
GAITVLGLAMFMDETDKNQDLKFDWFGFAALAVGIGAMQLALDRGEQLDWFNSPEIIIES
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
IVSVVGFYYFFAHSFTTERPFIQFAIFKDRNFVGGVVFMTVMGLVLFSTMALSSPFLQNV
HHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCC
EGYPIMTAGLLLASRGCGTFVAMMIVGRIMRYIEARTLIATGLSLTCASLYVMTGWTDQT
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHEEEEECCCCCH
SVSSVVVTSIIQGFGFGLVFVPLSTVAFMTLPGHLRTDGTAMLTLVRNVASSVGISVVIA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEE
KLSSGTTTAHAILSEHINPFNNALQMPNVTGMIDLSTTTGKAMMDAIVTMQAQIIAFSHD
ECCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEEECC
YAMVMFLTAAAVPMAIIIGSTKAALRKQSEAPADHAAVME
HHHHHHHHHHHHHHEEEECCCHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure 
AGAAQSHAVPGFRRNMVTICAMTATIMQALDTTIANVALPYMQGSLSASQDQINWVLTS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
YIVAAAIMTAPVGWIANRFGRKRIFIICSAGFTIASVMCGLAQDITQMVVFRLLQGVFGA
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVPLSQAVMLDSYALHERAKAMSIWGMGVMMGPIMGPSLGAWLTETYSWHWVFFVNLPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEEEEECCH
GAITVLGLAMFMDETDKNQDLKFDWFGFAALAVGIGAMQLALDRGEQLDWFNSPEIIIES
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
IVSVVGFYYFFAHSFTTERPFIQFAIFKDRNFVGGVVFMTVMGLVLFSTMALSSPFLQNV
HHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCC
EGYPIMTAGLLLASRGCGTFVAMMIVGRIMRYIEARTLIATGLSLTCASLYVMTGWTDQT
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHEEEEECCCCCH
SVSSVVVTSIIQGFGFGLVFVPLSTVAFMTLPGHLRTDGTAMLTLVRNVASSVGISVVIA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEE
KLSSGTTTAHAILSEHINPFNNALQMPNVTGMIDLSTTTGKAMMDAIVTMQAQIIAFSHD
ECCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEEECC
YAMVMFLTAAAVPMAIIIGSTKAALRKQSEAPADHAAVME
HHHHHHHHHHHHHHEEEECCCHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]