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The map label for this gene is truB [H]

Identifier: 192288869

GI number: 192288869

Start: 471506

End: 472606

Strand: Reverse

Name: truB [H]

Synonym: Rpal_0438

Alternate gene names: 192288869

Gene position: 472606-471506 (Counterclockwise)

Preceding gene: 192288870

Following gene: 192288868

Centisome position: 8.23

GC content: 68.3

Gene sequence:

>1101_bases
ATGACCGTGACCACCCCCGACGCCCTGCTCGCCCCGCACGACGTGCAGCACGCCGGCGCCGACGAATCCGCCGCGCAGAT
CCGTAAGCCGCGCGACAACAAGGACCCGCGCAATGCCAATCGCGGCGGCGGCAATGGCAAGCCGCGCCGCGACAAGCGCG
ACGTCCACGGCTGGGTGGTGCTGGACAAGCCGATCGGCATGACCTCGACCCAGGCGGTCGCGGTGCTGAAGCGGCTGTTC
TCGGCCAAGCGCGCCGGCCATGCCGGTACCCTCGATCCGCTGGCCTCCGGCGGCCTGCCGATCGCGATGGGCGAAGCCAC
CAAGACGGTGCCGTTCGTGATGGACGGCCGCAAGCGCTACCGCTTCACCGTGGCTTGGGGCGAGGAACGGGATACCGACG
ACACCGAGGGCCGCGCGGTCGCGACCAGCCCGGATCGGCCGACCGCCGAGGCGATCAGGGCGCTGCTGCCGCGCTTTACC
GGCGTGATCGAGCAGGTTCCGCCGCAATACTCGGCCGTGAAGATCCAGGGCGAGCGCGCCTACGACCTGGCACGCGACGG
CGAGGTCGTGCCGCTGGTCCCGCGTCCGGTCGAGATTCACGAATTAACCCTTGTGGAACATGGAGATGACGGCCGCTCGG
TGTTCGAGGCCGAGTGCGGCAAGGGAACCTATGTGCGGGCGCTCGCCCGTGACATGGGCCGGCTGCTCGGCTGCTACGGT
CATATCTGCGCGCTGCGCCGCACCGTGGTGGGGCCGTTCGACGAGTCGGACATGATTCCGCTGGAAGAACTACAGGCTTT
GTGCGATAGAGCGGCGTCCGGCGAGGGTAGCCTCGCCGACGCGCTACTGCCCGTTGAGACCGCGCTGGACGACATCCCGG
CACTGGCCGTCACTCGGGCTGATGCGGCAAGGCTCCATCGGGGCCAGGCCGTTTTGTTGCGCGGACGGGATGCGCCTCAT
TGTAGCGGCACAGTCTATGTCACGGTGGCAGGCCGGCTTCTGGCCCTCGCCGAAGTCGGCAATGGCGAGCTCATCCCCAA
GCGCGTGTTCAACCTCACCGGGCTGACTGCCAGCTCGGCCGTTCGCAAGGAAAGTATCTGA

Upstream 100 bases:

>100_bases
TCCGCATCGACGAACGTTTCGCCGAGGCCGAGCGGATCGACAAACTGCTGCGCTCGCCGGCAGTCCAGAAAGACCTCGAA
CCGAATTCGGACCAGGATTA

Downstream 100 bases:

>100_bases
CGATGTCGATTACCGCAGAACGCAAAGCGGAAGTCATCAAGACCAGCGCCACCAAGGCCGGTGATACCGGTTCGCCCGAG
GTTCAGGTCGCGATCCTGTC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTVTTPDALLAPHDVQHAGADESAAQIRKPRDNKDPRNANRGGGNGKPRRDKRDVHGWVVLDKPIGMTSTQAVAVLKRLF
SAKRAGHAGTLDPLASGGLPIAMGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGRAVATSPDRPTAEAIRALLPRFT
GVIEQVPPQYSAVKIQGERAYDLARDGEVVPLVPRPVEIHELTLVEHGDDGRSVFEAECGKGTYVRALARDMGRLLGCYG
HICALRRTVVGPFDESDMIPLEELQALCDRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPH
CSGTVYVTVAGRLLALAEVGNGELIPKRVFNLTGLTASSAVRKESI

Sequences:

>Translated_366_residues
MTVTTPDALLAPHDVQHAGADESAAQIRKPRDNKDPRNANRGGGNGKPRRDKRDVHGWVVLDKPIGMTSTQAVAVLKRLF
SAKRAGHAGTLDPLASGGLPIAMGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGRAVATSPDRPTAEAIRALLPRFT
GVIEQVPPQYSAVKIQGERAYDLARDGEVVPLVPRPVEIHELTLVEHGDDGRSVFEAECGKGTYVRALARDMGRLLGCYG
HICALRRTVVGPFDESDMIPLEELQALCDRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPH
CSGTVYVTVAGRLLALAEVGNGELIPKRVFNLTGLTASSAVRKESI
>Mature_365_residues
TVTTPDALLAPHDVQHAGADESAAQIRKPRDNKDPRNANRGGGNGKPRRDKRDVHGWVVLDKPIGMTSTQAVAVLKRLFS
AKRAGHAGTLDPLASGGLPIAMGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGRAVATSPDRPTAEAIRALLPRFTG
VIEQVPPQYSAVKIQGERAYDLARDGEVVPLVPRPVEIHELTLVEHGDDGRSVFEAECGKGTYVRALARDMGRLLGCYGH
ICALRRTVVGPFDESDMIPLEELQALCDRAASGEGSLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPHC
SGTVYVTVAGRLLALAEVGNGELIPKRVFNLTGLTASSAVRKESI

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=215, Percent_Identity=37.2093023255814, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI215599015, Length=264, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI4503337, Length=264, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI2367200, Length=311, Percent_Identity=38.5852090032154, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17553978, Length=274, Percent_Identity=26.2773722627737, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=249, Percent_Identity=31.3253012048193, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=132, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364189, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281364187, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281364185, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281364183, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI62471759, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17975520, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 39263; Mature: 39132

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTTPDALLAPHDVQHAGADESAAQIRKPRDNKDPRNANRGGGNGKPRRDKRDVHGWVV
CCCCCCCHHCCCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
LDKPIGMTSTQAVAVLKRLFSAKRAGHAGTLDPLASGGLPIAMGEATKTVPFVMDGRKRY
EECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCCCCEEECCCEEE
RFTVAWGEERDTDDTEGRAVATSPDRPTAEAIRALLPRFTGVIEQVPPQYSAVKIQGERA
EEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
YDLARDGEVVPLVPRPVEIHELTLVEHGDDGRSVFEAECGKGTYVRALARDMGRLLGCYG
CHHCCCCCEEEECCCCCCEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HICALRRTVVGPFDESDMIPLEELQALCDRAASGEGSLADALLPVETALDDIPALAVTRA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEHH
DAARLHRGQAVLLRGRDAPHCSGTVYVTVAGRLLALAEVGNGELIPKRVFNLTGLTASSA
HHHHHCCCCEEEEECCCCCCCCCEEEEEECCHHHEEEECCCCCCCHHHHHHHCCCCHHHH
VRKESI
HHHCCC
>Mature Secondary Structure 
TVTTPDALLAPHDVQHAGADESAAQIRKPRDNKDPRNANRGGGNGKPRRDKRDVHGWVV
CCCCCCHHCCCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
LDKPIGMTSTQAVAVLKRLFSAKRAGHAGTLDPLASGGLPIAMGEATKTVPFVMDGRKRY
EECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCCCCEEECCCEEE
RFTVAWGEERDTDDTEGRAVATSPDRPTAEAIRALLPRFTGVIEQVPPQYSAVKIQGERA
EEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
YDLARDGEVVPLVPRPVEIHELTLVEHGDDGRSVFEAECGKGTYVRALARDMGRLLGCYG
CHHCCCCCEEEECCCCCCEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HICALRRTVVGPFDESDMIPLEELQALCDRAASGEGSLADALLPVETALDDIPALAVTRA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEHH
DAARLHRGQAVLLRGRDAPHCSGTVYVTVAGRLLALAEVGNGELIPKRVFNLTGLTASSA
HHHHHCCCCEEEEECCCCCCCCCEEEEEECCHHHEEEECCCCCCCHHHHHHHCCCCHHHH
VRKESI
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA