Click here to switch to the map view.

The map label for this gene is folD [H]

Identifier: 192288848

GI number: 192288848

Start: 449315

End: 450202

Strand: Reverse

Name: folD [H]

Synonym: Rpal_0417

Alternate gene names: 192288848

Gene position: 450202-449315 (Counterclockwise)

Preceding gene: 192288849

Following gene: 192288842

Centisome position: 7.84

GC content: 68.24

Gene sequence:

>888_bases
ATGACCGCCCGGATCATCGACGGAAAAACCATCTCGGCCGAGGTGCGCGCCCGCGTCGCCGCAGAGGTCACGCGGCTGAA
GACCGACCACGGCATCACGCCGGGCCTGGCGGTGGTGCTGGTCGGCAACGACCCGGCTTCCGAGGTCTATGTCCGCTCCA
AGCACAAGCAGACCCAGGAGGCCGGCATGGCCTCGTTCGAGCACCGGCTGCCCGCCGACGTGCCGCAGGCCGAGCTGATG
GCGTTGATCGCAAAGCTCAATGCCGATCCGGCCGTGCACGGCATTCTGGTGCAATTGCCGCTGCCGAAGGGTCTCGACAG
CAACGCGGTGATCGACGCGATCGATCCTGCCAAGGATGTCGACGGCCTCAACCCGACCAATGCCGGCCGGCTGGCCTCCG
GCCTGTTCGCGCTGACGCCGTGCACGCCGCTCGGCAGCATCATCATGGCCAAGACTGTGCACGCTTCGCTCGAGGGCATG
AACGCGCTGGTGATCGGCCGCTCCAATCTGGTCGGCAAGCCGCTGGTGCAATTGCTGCTGAACGAGAACGCCACGGTGAC
GATCGCGCATTCGCGCACCCGCGACCTGCCGGCGCTGTGCCGCCAGGCCGATCTGGTGTTCGCGGCGGTCGGTAAGGCCG
AGATGGTGAAGGGCGACTGGATCAAGCCGGGCGCCACCGTGATCGACGTCGGCATCAACCGCACGCCGGGCAAGGACGGC
GGCAAGGACAAGCTGCTCGGCGACGTCGCCTTCGCCGAGGCCAAGGAGGTTGCCGGCGCCATCACCCCGGTGCCCGGCGG
CGTCGGCCTGATGACGGTGGCCTGCCTGCTGGTCAACACCGTCCGTGCCGCCTGCGCGATCCACGGCCTGCCTAAGCCGG
CGGTGTAA

Upstream 100 bases:

>100_bases
CGGGAGGCCCGCGAGGGCGATCCACCGTTCGCCTCTCCGTCCGGGGAGGCGTTGTCATTTCGATGCGGACGCATCGGCTG
GACCAGTCAAAGAGGACGAC

Downstream 100 bases:

>100_bases
GCGGGCGCGACTTGGCGGCTTACGCTGCTTCCTCCACATTCGTCATACGCGGGCTTGACCCGCGTATCCATCGCGCGCGC
GAGCGCGCGAAAGAAGCATT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTARIIDGKTISAEVRARVAAEVTRLKTDHGITPGLAVVLVGNDPASEVYVRSKHKQTQEAGMASFEHRLPADVPQAELM
ALIAKLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDVDGLNPTNAGRLASGLFALTPCTPLGSIIMAKTVHASLEGM
NALVIGRSNLVGKPLVQLLLNENATVTIAHSRTRDLPALCRQADLVFAAVGKAEMVKGDWIKPGATVIDVGINRTPGKDG
GKDKLLGDVAFAEAKEVAGAITPVPGGVGLMTVACLLVNTVRAACAIHGLPKPAV

Sequences:

>Translated_295_residues
MTARIIDGKTISAEVRARVAAEVTRLKTDHGITPGLAVVLVGNDPASEVYVRSKHKQTQEAGMASFEHRLPADVPQAELM
ALIAKLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDVDGLNPTNAGRLASGLFALTPCTPLGSIIMAKTVHASLEGM
NALVIGRSNLVGKPLVQLLLNENATVTIAHSRTRDLPALCRQADLVFAAVGKAEMVKGDWIKPGATVIDVGINRTPGKDG
GKDKLLGDVAFAEAKEVAGAITPVPGGVGLMTVACLLVNTVRAACAIHGLPKPAV
>Mature_294_residues
TARIIDGKTISAEVRARVAAEVTRLKTDHGITPGLAVVLVGNDPASEVYVRSKHKQTQEAGMASFEHRLPADVPQAELMA
LIAKLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDVDGLNPTNAGRLASGLFALTPCTPLGSIIMAKTVHASLEGMN
ALVIGRSNLVGKPLVQLLLNENATVTIAHSRTRDLPALCRQADLVFAAVGKAEMVKGDWIKPGATVIDVGINRTPGKDGG
KDKLLGDVAFAEAKEVAGAITPVPGGVGLMTVACLLVNTVRAACAIHGLPKPAV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=48.0968858131488, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=41.0169491525424, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=40.7534246575342, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI36796743, Length=212, Percent_Identity=30.188679245283, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1786741, Length=288, Percent_Identity=48.9583333333333, Blast_Score=253, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=43.8356164383562, Blast_Score=214, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=44.4055944055944, Blast_Score=241, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=43.4782608695652, Blast_Score=225, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=300, Percent_Identity=25.6666666666667, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=47.6027397260274, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=47.6027397260274, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=47.3867595818815, Blast_Score=241, Evalue=4e-64,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=47.3867595818815, Blast_Score=241, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=47.3867595818815, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=47.3867595818815, Blast_Score=241, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30537; Mature: 30406

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00766 THF_DHG_CYH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARIIDGKTISAEVRARVAAEVTRLKTDHGITPGLAVVLVGNDPASEVYVRSKHKQTQE
CCCEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHEEHHHHHHHHH
AGMASFEHRLPADVPQAELMALIAKLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEECCCCHHHC
DGLNPTNAGRLASGLFALTPCTPLGSIIMAKTVHASLEGMNALVIGRSNLVGKPLVQLLL
CCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
NENATVTIAHSRTRDLPALCRQADLVFAAVGKAEMVKGDWIKPGATVIDVGINRTPGKDG
CCCCEEEEECCCCCCHHHHHHHHHEEEEECCCHHCCCCCCCCCCCEEEEECCCCCCCCCC
GKDKLLGDVAFAEAKEVAGAITPVPGGVGLMTVACLLVNTVRAACAIHGLPKPAV
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TARIIDGKTISAEVRARVAAEVTRLKTDHGITPGLAVVLVGNDPASEVYVRSKHKQTQE
CCEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHEEHHHHHHHHH
AGMASFEHRLPADVPQAELMALIAKLNADPAVHGILVQLPLPKGLDSNAVIDAIDPAKDV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEECCCCHHHC
DGLNPTNAGRLASGLFALTPCTPLGSIIMAKTVHASLEGMNALVIGRSNLVGKPLVQLLL
CCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
NENATVTIAHSRTRDLPALCRQADLVFAAVGKAEMVKGDWIKPGATVIDVGINRTPGKDG
CCCCEEEEECCCCCCHHHHHHHHHEEEEECCCHHCCCCCCCCCCCEEEEECCCCCCCCCC
GKDKLLGDVAFAEAKEVAGAITPVPGGVGLMTVACLLVNTVRAACAIHGLPKPAV
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA