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The map label for this gene is trmE [H]

Identifier: 192288728

GI number: 192288728

Start: 318978

End: 320303

Strand: Reverse

Name: trmE [H]

Synonym: Rpal_0297

Alternate gene names: 192288728

Gene position: 320303-318978 (Counterclockwise)

Preceding gene: 192288729

Following gene: 192288727

Centisome position: 5.58

GC content: 66.52

Gene sequence:

>1326_bases
ATGCATCCGAGCGACCAGACCATCTTCGCCCTTGCGACCGGACCGCTGCCGTCGGCAATCGCCATCCTGCGTGTCTCCGG
ATCGCGAGCGGGCGACGTGCTGAGAGCGCTGACCGGATCATTACCGCCGCCCCGGCGCGCGGTGCGCTGTGATCTTCGGA
GCCGAGATGGCGATCTGATCGATGACGGCGTGGCGTTGTGGTTTCCGACGCCGGCGTCTGCCACCGGCGAAGATGTTGCC
GAATTGCACATCCACGGCAGCCGTGCGGTGGCGGCGGCGCTGATCAAGACCCTCTCGGCATTCGAAGGCGTGCGGCCGGC
GGAGCCGGGCGAGTTCACCCGGCGTGGATTCGAAAACGGCAAGCTCGACCTGACCGAAGCCGAAGGCCTCGACGATCTGA
TCCATGCGGACACCGACGCGCAGCGGCGTCAGGCGCTGCGCCAGCTCGGCGGCGTGCTGGGCGACCGGGCGCGGCAATGG
CGCGACCAGATCATCGAGGCACTGGCACTGGTCGAAGCGGGGATCGACTTTTCCGACGAAGGCGACGTTGCCGACGAGTT
GATGGGGCCGGCGCGGGCGAAGATCGCAGAGCTGTCCGCAGAGATCGCCGAGGTCTTGGCCGAGCAGGGCCGGGGCGAAA
AGCTCCGCGACGGGATGGTGGTGGCGATCGCCGGGCCGCCGAACGTCGGCAAGTCCACTCTGATCAATCGCCTGGCGCGC
CGCGAGGTGGCGATCGTCTCGCCGCATGCCGGCACCACCCGGGATGTGATCGAAATCCAGCTCGATCTCGACGGCTATCC
GGTGACGGTGATCGATACGGCGGGACTGCGAGACAGTGACGATCCGGTCGAGCAGGAGGGCGTCCGCCGCGCTCGCTTGC
GAGCCGCGGCGGCGGATCTGGTGCTGTGGCTGAGCACGGCGACGGACGCTTCTGATCCGGATGTGAAAGGTCCGGAGGTC
TGGCGGGTCCGGAATAAGATCGATTTGGCGAACGGCGAGGTGGCAGAATCCGGCCCGTCACAGCCCGTTTTCCGGATTTC
GGCCGCGACCGGCGAGGGGTTTGCGGATTTGCTGCGCGAACTGACGCGATTTGCTGCGCAGTATTTCGGGTCCGCAGAAG
CCGGATTGATCACGCGCGACCGCCATCGGCGGTTGCTCGCCGATGCTGCGGCATCGCTGACACGCAGTCTCGTGCCGGGA
CTGGCCGAAGAAATCGTCGCCGAAGAACTCCGCGCTTCGGCTCACAGTCTCGGCCGATTGCTCGGGCGGGTCGACGTCGA
GGATGTCCTTGGTGAGATCTTCGGCCGGTTCTGCATTGGCAAATAG

Upstream 100 bases:

>100_bases
GATGAACACCTGAGCGAGTCGAATAGCCGGCCATCAAGGGCGTCCCCACGGGGCGCCCTTTTTGTTTGCGTTCGGAACAG
CGCCTCGGCAAGACAGCGCC

Downstream 100 bases:

>100_bases
GCTTGCTCGGGTCTCGGCCGGTCAGCCGGTGGTAACGAAACCTTAACCACGCTATCGGGATTTGGGCTCCGCTGTTTCAC
GTGAAACAGTTCGGTGAGGT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MHPSDQTIFALATGPLPSAIAILRVSGSRAGDVLRALTGSLPPPRRAVRCDLRSRDGDLIDDGVALWFPTPASATGEDVA
ELHIHGSRAVAAALIKTLSAFEGVRPAEPGEFTRRGFENGKLDLTEAEGLDDLIHADTDAQRRQALRQLGGVLGDRARQW
RDQIIEALALVEAGIDFSDEGDVADELMGPARAKIAELSAEIAEVLAEQGRGEKLRDGMVVAIAGPPNVGKSTLINRLAR
REVAIVSPHAGTTRDVIEIQLDLDGYPVTVIDTAGLRDSDDPVEQEGVRRARLRAAAADLVLWLSTATDASDPDVKGPEV
WRVRNKIDLANGEVAESGPSQPVFRISAATGEGFADLLRELTRFAAQYFGSAEAGLITRDRHRRLLADAAASLTRSLVPG
LAEEIVAEELRASAHSLGRLLGRVDVEDVLGEIFGRFCIGK

Sequences:

>Translated_441_residues
MHPSDQTIFALATGPLPSAIAILRVSGSRAGDVLRALTGSLPPPRRAVRCDLRSRDGDLIDDGVALWFPTPASATGEDVA
ELHIHGSRAVAAALIKTLSAFEGVRPAEPGEFTRRGFENGKLDLTEAEGLDDLIHADTDAQRRQALRQLGGVLGDRARQW
RDQIIEALALVEAGIDFSDEGDVADELMGPARAKIAELSAEIAEVLAEQGRGEKLRDGMVVAIAGPPNVGKSTLINRLAR
REVAIVSPHAGTTRDVIEIQLDLDGYPVTVIDTAGLRDSDDPVEQEGVRRARLRAAAADLVLWLSTATDASDPDVKGPEV
WRVRNKIDLANGEVAESGPSQPVFRISAATGEGFADLLRELTRFAAQYFGSAEAGLITRDRHRRLLADAAASLTRSLVPG
LAEEIVAEELRASAHSLGRLLGRVDVEDVLGEIFGRFCIGK
>Mature_441_residues
MHPSDQTIFALATGPLPSAIAILRVSGSRAGDVLRALTGSLPPPRRAVRCDLRSRDGDLIDDGVALWFPTPASATGEDVA
ELHIHGSRAVAAALIKTLSAFEGVRPAEPGEFTRRGFENGKLDLTEAEGLDDLIHADTDAQRRQALRQLGGVLGDRARQW
RDQIIEALALVEAGIDFSDEGDVADELMGPARAKIAELSAEIAEVLAEQGRGEKLRDGMVVAIAGPPNVGKSTLINRLAR
REVAIVSPHAGTTRDVIEIQLDLDGYPVTVIDTAGLRDSDDPVEQEGVRRARLRAAAADLVLWLSTATDASDPDVKGPEV
WRVRNKIDLANGEVAESGPSQPVFRISAATGEGFADLLRELTRFAAQYFGSAEAGLITRDRHRRLLADAAASLTRSLVPG
LAEEIVAEELRASAHSLGRLLGRVDVEDVLGEIFGRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=43.8709677419355, Blast_Score=308, Evalue=4e-84,
Organism=Homo sapiens, GI193082993, Length=447, Percent_Identity=44.0715883668904, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=41.2474849094567, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI2367268, Length=453, Percent_Identity=40.6181015452539, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI87082120, Length=169, Percent_Identity=34.3195266272189, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=448, Percent_Identity=33.9285714285714, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6323665, Length=496, Percent_Identity=32.8629032258064, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI45550813, Length=475, Percent_Identity=38.7368421052632, Blast_Score=285, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 47143; Mature: 47143

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPSDQTIFALATGPLPSAIAILRVSGSRAGDVLRALTGSLPPPRRAVRCDLRSRDGDLI
CCCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCHHHEECCCCCCCCEE
DDGVALWFPTPASATGEDVAELHIHGSRAVAAALIKTLSAFEGVRPAEPGEFTRRGFENG
CCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
KLDLTEAEGLDDLIHADTDAQRRQALRQLGGVLGDRARQWRDQIIEALALVEAGIDFSDE
CEEECHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GDVADELMGPARAKIAELSAEIAEVLAEQGRGEKLRDGMVVAIAGPPNVGKSTLINRLAR
CCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
REVAIVSPHAGTTRDVIEIQLDLDGYPVTVIDTAGLRDSDDPVEQEGVRRARLRAAAADL
CCEEEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLWLSTATDASDPDVKGPEVWRVRNKIDLANGEVAESGPSQPVFRISAATGEGFADLLRE
HHEEECCCCCCCCCCCCCHHEEECCCEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHH
LTRFAAQYFGSAEAGLITRDRHRRLLADAAASLTRSLVPGLAEEIVAEELRASAHSLGRL
HHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRVDVEDVLGEIFGRFCIGK
HHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHPSDQTIFALATGPLPSAIAILRVSGSRAGDVLRALTGSLPPPRRAVRCDLRSRDGDLI
CCCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCHHHEECCCCCCCCEE
DDGVALWFPTPASATGEDVAELHIHGSRAVAAALIKTLSAFEGVRPAEPGEFTRRGFENG
CCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
KLDLTEAEGLDDLIHADTDAQRRQALRQLGGVLGDRARQWRDQIIEALALVEAGIDFSDE
CEEECHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GDVADELMGPARAKIAELSAEIAEVLAEQGRGEKLRDGMVVAIAGPPNVGKSTLINRLAR
CCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
REVAIVSPHAGTTRDVIEIQLDLDGYPVTVIDTAGLRDSDDPVEQEGVRRARLRAAAADL
CCEEEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLWLSTATDASDPDVKGPEVWRVRNKIDLANGEVAESGPSQPVFRISAATGEGFADLLRE
HHEEECCCCCCCCCCCCCHHEEECCCEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHH
LTRFAAQYFGSAEAGLITRDRHRRLLADAAASLTRSLVPGLAEEIVAEELRASAHSLGRL
HHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRVDVEDVLGEIFGRFCIGK
HHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA