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The map label for this gene is mnmG [H]

Identifier: 192288727

GI number: 192288727

Start: 316944

End: 318812

Strand: Reverse

Name: mnmG [H]

Synonym: Rpal_0296

Alternate gene names: 192288727

Gene position: 318812-316944 (Counterclockwise)

Preceding gene: 192288728

Following gene: 192288726

Centisome position: 5.55

GC content: 64.63

Gene sequence:

>1869_bases
ATGCGCACCTCTTTCGACGTCATCGTCATCGGCGGCGGCCATGCCGGCTGCGAAGCTGCGGCTGCGGCTGCCCGGATCGG
CGCGGCTACCGCCCTCGTCACCCATCGTTTCGCCACCGTAGGTGCGATGTCCTGCAATCCGGCGATCGGAGGGCTGGGCA
AAGGTCATCTGGTCCGCGAAGTGGATGCCTTGGATGGGCTGATGGGCCGGGTCGCCGACGCCGGCGGCATTCAGTTTCGG
ATGCTGAACCGCCGTAAAGGCCCCGCGGTCCGCGGCCCGCGGGCGCAGGCTGATCGCAAGCTGTATGCGGCCGCGATGCA
GATCGCGATCCAGGGCTTTCCGAATTTGTCGGTGATCGAAGGGGAGGCCGACGCGCTGCTATGGCAGGACGGCCGTGTGA
GCGGCATTCGGCTCGGCGACGGCCGGGAGTTTGCTGCCGCGGCGGTCGTCATTACGACCGGGACCTTTCTGCGCGGCCTC
ATTCACCTCGGCGAGCGAAGCTGGCCCGCTGGCCGGATCGACGAGGCCCCAGCGATGGGGCTTTCATCGTCGTTCGAAGC
GCTCGGATTTAAGCTCGGTCGCCTCAAAACCGGAACTCCGCCACGTCTGGACGGCCGGACAATCGACTGGAGCGCCGTCG
AAATGCAGCCCGGCGATGATCCGGCGGAACCGTTTTCGGTCCTGACGCCGGCAATCACCACGCCGCAGATCGAATGCGGC
ATCACCCGGACGACGCCGGCCACCCATGAGGTGATCCGAGCCAATGTGCATCGCTCGCCGATGTATTCAGGACAGATCCA
GTCGACTGGGCCGCGTTATTGCCCCTCGATTGAGGACAAGATCGTCAAATTCGGCGATCGCGACGGCCACCAGATCTTCC
TGGAGCCGGAAGGGCTTGATGATCCGACCGTCTATCCCAACGGGATCTCAACTTCGCTTCCCGAAGAGGTTCAGCGGGCA
ATCCTGAAGACCATTCCGGGGCTGGAGCGAACCGAAATGCTCCGTCCGGGGTACGCGATTGAATACGATCACGTCGACCC
GCGGGAGTTGGAGCCGACGCTGCAGACCAAGCGGCTGCGCGGGCTGTTCCTGGCCGGGCAGATCAATGGCACCACCGGTT
ATGAAGAGGCGGCAGCCCAGGGTCTGGTCGCTGGGCTCAATGCGGCATTGTCCGCGGGAGGTGGCGATCGGGCGGTGTTC
GATCGCGCCGACGGCTACCTCGGCGTGATGATCGACGATCTGGTGACCCGCGGCATCACCGAGCCGTACCGGATGTTTAC
CTCCCGGGCCGAGTATCGCCTGACCCTTCGGGCCGACAACGCCGACCAGCGTCTGACCGACAAGGGCCTAGCACTCGGTT
GCGTCGGGGTGGAGCGGTCGGCCTTCCATCATGACAAAATGGCCGCACTTTCGGACGCGAAAGCGCTTGCCCAATCGCTG
TCGATCACCCCGAACGAAGCAGCCAAGCACGGACTGGCACTGAACCGGGATGGCCAGCGCCGCTCCGCGTTCGATCTGTT
GTCCTATCCGGAAATCGAGTGGGCTCAGGTTCGCGCGATATGGCCGGAGCTTGGAAAGGTCGATCCCGCGATCGCGGTGC
ATGTCGAGATCGACGCCAAATATCATGTCTATCTGGAGCGCCAGACCGCAGACGTCGAGGCGTTTCGTCGGGATGAAAGT
CTCGGGCTTACCGATGTCGATTACGCGGCGGTCCCCGGTCTGTCGAACGAGGCCCGCACGCGGCTGGAGCGGCATCGCCC
ACACACCGTCGGGCAGGCGGGTCGGCTCGACGGCATCACGCCAGCGGCCCTCGGAATTCTCGCCGCCTATCTGCGGCGTG
AACAACGGAAGCGGAAATCGACGGGGTAG

Upstream 100 bases:

>100_bases
GCTCCGCTGTTTCACGTGAAACAGTTCGGTGAGGTTCAGCCGGTATCAAGATCGGCGAACTCGCCTTCAAAGCTCCCCGG
AGCTGAGCTACAACCCCACC

Downstream 100 bases:

>100_bases
GGTAGCAACCAGCGTTTCACGTGAAACACCTCCACCTCTCGGCTCCGTCGTGTTTCACGTGAAACTCTAGCCGTCGATTG
CAGACCGGTGCCGTATTCGG

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MRTSFDVIVIGGGHAGCEAAAAAARIGAATALVTHRFATVGAMSCNPAIGGLGKGHLVREVDALDGLMGRVADAGGIQFR
MLNRRKGPAVRGPRAQADRKLYAAAMQIAIQGFPNLSVIEGEADALLWQDGRVSGIRLGDGREFAAAAVVITTGTFLRGL
IHLGERSWPAGRIDEAPAMGLSSSFEALGFKLGRLKTGTPPRLDGRTIDWSAVEMQPGDDPAEPFSVLTPAITTPQIECG
ITRTTPATHEVIRANVHRSPMYSGQIQSTGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLDDPTVYPNGISTSLPEEVQRA
ILKTIPGLERTEMLRPGYAIEYDHVDPRELEPTLQTKRLRGLFLAGQINGTTGYEEAAAQGLVAGLNAALSAGGGDRAVF
DRADGYLGVMIDDLVTRGITEPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGVERSAFHHDKMAALSDAKALAQSL
SITPNEAAKHGLALNRDGQRRSAFDLLSYPEIEWAQVRAIWPELGKVDPAIAVHVEIDAKYHVYLERQTADVEAFRRDES
LGLTDVDYAAVPGLSNEARTRLERHRPHTVGQAGRLDGITPAALGILAAYLRREQRKRKSTG

Sequences:

>Translated_622_residues
MRTSFDVIVIGGGHAGCEAAAAAARIGAATALVTHRFATVGAMSCNPAIGGLGKGHLVREVDALDGLMGRVADAGGIQFR
MLNRRKGPAVRGPRAQADRKLYAAAMQIAIQGFPNLSVIEGEADALLWQDGRVSGIRLGDGREFAAAAVVITTGTFLRGL
IHLGERSWPAGRIDEAPAMGLSSSFEALGFKLGRLKTGTPPRLDGRTIDWSAVEMQPGDDPAEPFSVLTPAITTPQIECG
ITRTTPATHEVIRANVHRSPMYSGQIQSTGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLDDPTVYPNGISTSLPEEVQRA
ILKTIPGLERTEMLRPGYAIEYDHVDPRELEPTLQTKRLRGLFLAGQINGTTGYEEAAAQGLVAGLNAALSAGGGDRAVF
DRADGYLGVMIDDLVTRGITEPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGVERSAFHHDKMAALSDAKALAQSL
SITPNEAAKHGLALNRDGQRRSAFDLLSYPEIEWAQVRAIWPELGKVDPAIAVHVEIDAKYHVYLERQTADVEAFRRDES
LGLTDVDYAAVPGLSNEARTRLERHRPHTVGQAGRLDGITPAALGILAAYLRREQRKRKSTG
>Mature_622_residues
MRTSFDVIVIGGGHAGCEAAAAAARIGAATALVTHRFATVGAMSCNPAIGGLGKGHLVREVDALDGLMGRVADAGGIQFR
MLNRRKGPAVRGPRAQADRKLYAAAMQIAIQGFPNLSVIEGEADALLWQDGRVSGIRLGDGREFAAAAVVITTGTFLRGL
IHLGERSWPAGRIDEAPAMGLSSSFEALGFKLGRLKTGTPPRLDGRTIDWSAVEMQPGDDPAEPFSVLTPAITTPQIECG
ITRTTPATHEVIRANVHRSPMYSGQIQSTGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLDDPTVYPNGISTSLPEEVQRA
ILKTIPGLERTEMLRPGYAIEYDHVDPRELEPTLQTKRLRGLFLAGQINGTTGYEEAAAQGLVAGLNAALSAGGGDRAVF
DRADGYLGVMIDDLVTRGITEPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGVERSAFHHDKMAALSDAKALAQSL
SITPNEAAKHGLALNRDGQRRSAFDLLSYPEIEWAQVRAIWPELGKVDPAIAVHVEIDAKYHVYLERQTADVEAFRRDES
LGLTDVDYAAVPGLSNEARTRLERHRPHTVGQAGRLDGITPAALGILAAYLRREQRKRKSTG

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=640, Percent_Identity=44.53125, Blast_Score=521, Evalue=1e-148,
Organism=Homo sapiens, GI19882217, Length=665, Percent_Identity=42.8571428571429, Blast_Score=506, Evalue=1e-143,
Organism=Homo sapiens, GI183227703, Length=680, Percent_Identity=41.9117647058824, Blast_Score=502, Evalue=1e-142,
Organism=Escherichia coli, GI2367273, Length=625, Percent_Identity=51.2, Blast_Score=599, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI17534255, Length=624, Percent_Identity=43.5897435897436, Blast_Score=496, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6321202, Length=625, Percent_Identity=47.68, Blast_Score=558, Evalue=1e-160,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=46.9096671949287, Blast_Score=524, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 67085; Mature: 67085

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSFDVIVIGGGHAGCEAAAAAARIGAATALVTHRFATVGAMSCNPAIGGLGKGHLVRE
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
VDALDGLMGRVADAGGIQFRMLNRRKGPAVRGPRAQADRKLYAAAMQIAIQGFPNLSVIE
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
GEADALLWQDGRVSGIRLGDGREFAAAAVVITTGTFLRGLIHLGERSWPAGRIDEAPAMG
CCCCEEEEECCCEEEEEECCCCCEEEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCC
LSSSFEALGFKLGRLKTGTPPRLDGRTIDWSAVEMQPGDDPAEPFSVLTPAITTPQIECG
CCCCHHHHCEECCCCCCCCCCCCCCCEECCEEEEECCCCCCCCCHHHHCCCCCCCCEEEC
ITRTTPATHEVIRANVHRSPMYSGQIQSTGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLD
EECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCC
DPTVYPNGISTSLPEEVQRAILKTIPGLERTEMLRPGYAIEYDHVDPRELEPTLQTKRLR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCEEEECCCCHHHCCCHHHHHHHC
GLFLAGQINGTTGYEEAAAQGLVAGLNAALSAGGGDRAVFDRADGYLGVMIDDLVTRGIT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEHHHHHHHHHCCC
EPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGVERSAFHHDKMAALSDAKALAQSL
HHHHHHHCCCEEEEEEEECCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHC
SITPNEAAKHGLALNRDGQRRSAFDLLSYPEIEWAQVRAIWPELGKVDPAIAVHVEIDAK
CCCCCHHHHCCEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCE
YHVYLERQTADVEAFRRDESLGLTDVDYAAVPGLSNEARTRLERHRPHTVGQAGRLDGIT
EEEEEECCCCCHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PAALGILAAYLRREQRKRKSTG
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRTSFDVIVIGGGHAGCEAAAAAARIGAATALVTHRFATVGAMSCNPAIGGLGKGHLVRE
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
VDALDGLMGRVADAGGIQFRMLNRRKGPAVRGPRAQADRKLYAAAMQIAIQGFPNLSVIE
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
GEADALLWQDGRVSGIRLGDGREFAAAAVVITTGTFLRGLIHLGERSWPAGRIDEAPAMG
CCCCEEEEECCCEEEEEECCCCCEEEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCC
LSSSFEALGFKLGRLKTGTPPRLDGRTIDWSAVEMQPGDDPAEPFSVLTPAITTPQIECG
CCCCHHHHCEECCCCCCCCCCCCCCCEECCEEEEECCCCCCCCCHHHHCCCCCCCCEEEC
ITRTTPATHEVIRANVHRSPMYSGQIQSTGPRYCPSIEDKIVKFGDRDGHQIFLEPEGLD
EECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCC
DPTVYPNGISTSLPEEVQRAILKTIPGLERTEMLRPGYAIEYDHVDPRELEPTLQTKRLR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCEEEECCCCHHHCCCHHHHHHHC
GLFLAGQINGTTGYEEAAAQGLVAGLNAALSAGGGDRAVFDRADGYLGVMIDDLVTRGIT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEHHHHHHHHHCCC
EPYRMFTSRAEYRLTLRADNADQRLTDKGLALGCVGVERSAFHHDKMAALSDAKALAQSL
HHHHHHHCCCEEEEEEEECCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHC
SITPNEAAKHGLALNRDGQRRSAFDLLSYPEIEWAQVRAIWPELGKVDPAIAVHVEIDAK
CCCCCHHHHCCEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCE
YHVYLERQTADVEAFRRDESLGLTDVDYAAVPGLSNEARTRLERHRPHTVGQAGRLDGIT
EEEEEECCCCCHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PAALGILAAYLRREQRKRKSTG
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA