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The map label for this gene is lpd3 [H]

Identifier: 192288613

GI number: 192288613

Start: 195842

End: 197245

Strand: Reverse

Name: lpd3 [H]

Synonym: Rpal_0180

Alternate gene names: 192288613

Gene position: 197245-195842 (Counterclockwise)

Preceding gene: 192288614

Following gene: 192288612

Centisome position: 3.43

GC content: 65.74

Gene sequence:

>1404_bases
ATGGCCACCTACGATCTCGTCGTCATCGGCACCGGCCCCGGCGGCTACGTCTGCGCGATTCGCGCGGCGCAACTCGGCCT
CAAGGTGGCGGTGGTCGAGAAAAACGCCACGCTCGGCGGCACCTGCCTGAACGTCGGCTGCATGCCGTCGAAGGCGCTGC
TGCATGCCTCCGAACTGTTCGAGGAAGCCGGGCATTCGTTCGCCAAGATGGGCATCGGCGTGCCGGCCCCGAAGCTCGAT
CTACCGGCGATGATGAACTTCAAGCAGCAGGGTATCGACGGCAACGTCAAAGGCGTCGAATACCTGATGAAGAAGAACAA
GATCGACGTGCTGGTCGGCAAGGGCAAGATCCTCGGCACCGGCAAGGTCGAGGTGACGGGCGCCGACGGCAAGGCCTCCA
GCGTCGACACCAAGTCGATCGTGATCGCCTCCGGCTCGGCTGTGGCTCAGCTCAAGGGCATCGAGATCGATGAGAAGCGC
GTGGTGTCGTCCACCGGCGCGCTGTCGCTCGACAAGGTCCCGGGCAAGCTGATCGTTGTCGGCGCCGGCGTGATCGGGCT
CGAGCTTGGCTCGGTGTGGCGGCGCCTCGGCGCCGAGGTCACCGTGGTCGAATTCCTCGACCGCATCTTGCCGGGCATGG
ACGGCGAGGTGGTCAAGCAATTCCAGCGCATCCTCGAGAAGCAGGGCTTTGCCTTCAAGCTCGGCGCCAAGGTCACCGGT
GTCGACACCTCGGGCGCCAAGCTTGCCGTGAAGGTCGAGGCGGCGGCCGGTGGCAATCCGGAAACGCTGGAAGCCGACGT
GGTGCTGGTCGCGATCGGCCGCGTCCCCTACACCGAAGGCCTCGGCCTAAAGGAGGCCGGCGTTGCGCTCGACGAGCGCG
GCCGGGTGGTGATCGACGACCACTTCGCCACCAGCCTCAAGGGCGTGTACGCGATCGGCGACGTGGTGCGCGGCCCGATG
CTGGCCCATAAGGCCGAGGACGAAGGCGTTGCGGTTGCCGAGTTGATCGCCGGCAAGGCCGGCCACGTCAATTACGACGT
CATTCCGGGCGTGGTCTACACGACGCCGGAAGTGTCGAGCGTCGGCAAGACCGAAGAGGACCTCAAGCAGGCCGGCGTCG
CCTATACGGTCGGGAAATTCCCGTTCACCGCCAACGGCCGCTCCAAGGTCAACCAGACCACCGACGGCTTTGTGAAGATC
TTGGCCGACGCCAAGACCGACCGTGTTCTCGGCGTCCACATCATCGGCCGTGAGGCCGGCGAGATGATCCACGAAGCGGC
CGTGCTGATGGAGTTCGGCGGCTCTGCCGAAGATCTGGCCCGCACCTGCCACGCCCATCCGACCCGCTCGGAGGCCATCA
AGGAGGCCGCCCTTACGGTCGGCAAGCGCGCCATCCATATGTGA

Upstream 100 bases:

>100_bases
GTCTGGCTGCTGTCCGACGAGGCCTCCTATGTCACCAGCGCCATCATCGACGTGTCGGGGGGACGATAGTCGCGCCGGCG
CCAACTCACAGGATTGAACT

Downstream 100 bases:

>100_bases
CGGGAGCCGGCGCGAGGCCGGCCTACGGATGGTGGCGTGATGAAAGGTCGGTTGTGGCAGCCCCTGTGGGTGCTGCTGGC
GGTTCTGTTCCTGATCGAGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MATYDLVVIGTGPGGYVCAIRAAQLGLKVAVVEKNATLGGTCLNVGCMPSKALLHASELFEEAGHSFAKMGIGVPAPKLD
LPAMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGKGKILGTGKVEVTGADGKASSVDTKSIVIASGSAVAQLKGIEIDEKR
VVSSTGALSLDKVPGKLIVVGAGVIGLELGSVWRRLGAEVTVVEFLDRILPGMDGEVVKQFQRILEKQGFAFKLGAKVTG
VDTSGAKLAVKVEAAAGGNPETLEADVVLVAIGRVPYTEGLGLKEAGVALDERGRVVIDDHFATSLKGVYAIGDVVRGPM
LAHKAEDEGVAVAELIAGKAGHVNYDVIPGVVYTTPEVSSVGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGFVKI
LADAKTDRVLGVHIIGREAGEMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAIKEAALTVGKRAIHM

Sequences:

>Translated_467_residues
MATYDLVVIGTGPGGYVCAIRAAQLGLKVAVVEKNATLGGTCLNVGCMPSKALLHASELFEEAGHSFAKMGIGVPAPKLD
LPAMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGKGKILGTGKVEVTGADGKASSVDTKSIVIASGSAVAQLKGIEIDEKR
VVSSTGALSLDKVPGKLIVVGAGVIGLELGSVWRRLGAEVTVVEFLDRILPGMDGEVVKQFQRILEKQGFAFKLGAKVTG
VDTSGAKLAVKVEAAAGGNPETLEADVVLVAIGRVPYTEGLGLKEAGVALDERGRVVIDDHFATSLKGVYAIGDVVRGPM
LAHKAEDEGVAVAELIAGKAGHVNYDVIPGVVYTTPEVSSVGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGFVKI
LADAKTDRVLGVHIIGREAGEMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAIKEAALTVGKRAIHM
>Mature_466_residues
ATYDLVVIGTGPGGYVCAIRAAQLGLKVAVVEKNATLGGTCLNVGCMPSKALLHASELFEEAGHSFAKMGIGVPAPKLDL
PAMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGKGKILGTGKVEVTGADGKASSVDTKSIVIASGSAVAQLKGIEIDEKRV
VSSTGALSLDKVPGKLIVVGAGVIGLELGSVWRRLGAEVTVVEFLDRILPGMDGEVVKQFQRILEKQGFAFKLGAKVTGV
DTSGAKLAVKVEAAAGGNPETLEADVVLVAIGRVPYTEGLGLKEAGVALDERGRVVIDDHFATSLKGVYAIGDVVRGPML
AHKAEDEGVAVAELIAGKAGHVNYDVIPGVVYTTPEVSSVGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGFVKIL
ADAKTDRVLGVHIIGREAGEMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAIKEAALTVGKRAIHM

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=457, Percent_Identity=56.6739606126915, Blast_Score=509, Evalue=1e-144,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=28.1115879828326, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI22035672, Length=473, Percent_Identity=30.0211416490486, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI33519430, Length=471, Percent_Identity=28.4501061571125, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI33519428, Length=471, Percent_Identity=28.4501061571125, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI33519426, Length=471, Percent_Identity=28.4501061571125, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI148277071, Length=471, Percent_Identity=28.4501061571125, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI148277065, Length=471, Percent_Identity=28.4501061571125, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI291045266, Length=464, Percent_Identity=28.8793103448276, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI291045268, Length=458, Percent_Identity=27.7292576419214, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI1786307, Length=453, Percent_Identity=41.5011037527594, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=30.6034482758621, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=29.5652173913043, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1789915, Length=443, Percent_Identity=29.5711060948081, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=455, Percent_Identity=57.8021978021978, Blast_Score=521, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17557007, Length=493, Percent_Identity=30.6288032454361, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71982272, Length=492, Percent_Identity=26.0162601626016, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=26.9230769230769, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=26.9230769230769, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=231, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=51.7970401691332, Blast_Score=461, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=35.031847133758, Blast_Score=260, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=27.5268817204301, Blast_Score=152, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21358499, Length=455, Percent_Identity=56.9230769230769, Blast_Score=507, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24640553, Length=477, Percent_Identity=30.6079664570231, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24640549, Length=477, Percent_Identity=30.6079664570231, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=30.6418219461698, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17737741, Length=482, Percent_Identity=27.3858921161826, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48598; Mature: 48467

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATYDLVVIGTGPGGYVCAIRAAQLGLKVAVVEKNATLGGTCLNVGCMPSKALLHASELF
CCEEEEEEEECCCCCEEEEEEEHHCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHH
EEAGHSFAKMGIGVPAPKLDLPAMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGKGKILGT
HHHCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCEEEEC
GKVEVTGADGKASSVDTKSIVIASGSAVAQLKGIEIDEKRVVSSTGALSLDKVPGKLIVV
CEEEEECCCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHCCCCEEECCCCCEEEEE
GAGVIGLELGSVWRRLGAEVTVVEFLDRILPGMDGEVVKQFQRILEKQGFAFKLGAKVTG
ECCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEEE
VDTSGAKLAVKVEAAAGGNPETLEADVVLVAIGRVPYTEGLGLKEAGVALDERGRVVIDD
CCCCCCEEEEEEEECCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEC
HFATSLKGVYAIGDVVRGPMLAHKAEDEGVAVAELIAGKAGHVNYDVIPGVVYTTPEVSS
CHHHHHCHHHHHHHHHCCCHHEECCCCCCCCHHHHHCCCCCCCCEEECCCEEEECCCCHH
VGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGFVKILADAKTDRVLGVHIIGREAG
CCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHEEEECCCCCCEEEEEEECCHHH
EMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAIKEAALTVGKRAIHM
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATYDLVVIGTGPGGYVCAIRAAQLGLKVAVVEKNATLGGTCLNVGCMPSKALLHASELF
CEEEEEEEECCCCCEEEEEEEHHCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHH
EEAGHSFAKMGIGVPAPKLDLPAMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGKGKILGT
HHHCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCEEEEC
GKVEVTGADGKASSVDTKSIVIASGSAVAQLKGIEIDEKRVVSSTGALSLDKVPGKLIVV
CEEEEECCCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHCCCCEEECCCCCEEEEE
GAGVIGLELGSVWRRLGAEVTVVEFLDRILPGMDGEVVKQFQRILEKQGFAFKLGAKVTG
ECCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEEE
VDTSGAKLAVKVEAAAGGNPETLEADVVLVAIGRVPYTEGLGLKEAGVALDERGRVVIDD
CCCCCCEEEEEEEECCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEC
HFATSLKGVYAIGDVVRGPMLAHKAEDEGVAVAELIAGKAGHVNYDVIPGVVYTTPEVSS
CHHHHHCHHHHHHHHHCCCHHEECCCCCCCCHHHHHCCCCCCCCEEECCCEEEECCCCHH
VGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGFVKILADAKTDRVLGVHIIGREAG
CCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHEEEECCCCCCEEEEEEECCHHH
EMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAIKEAALTVGKRAIHM
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1722146; 2914869 [H]