Click here to switch to the map view.

The map label for this gene is bznB [H]

Identifier: 192288545

GI number: 192288545

Start: 123664

End: 125412

Strand: Reverse

Name: bznB [H]

Synonym: Rpal_0112

Alternate gene names: 192288545

Gene position: 125412-123664 (Counterclockwise)

Preceding gene: 192288546

Following gene: 192288544

Centisome position: 2.18

GC content: 66.78

Gene sequence:

>1749_bases
ATGTCCGCAGCGACTTCGTCGGCCGGCGACTGGCTCGAGGGCGGCGAACTGCTCGCCCGCACACTTCGTGACGCCGGCGT
CGACACCGTGTTCGCCCTGCACGGCGGTCATCTCGAATCCTTCTATCGTGGCTGCATCGCCAACGACATCCGGCTGGTCG
ATTTCCGCCACGAATCCTCGGCCGGCCACGCCGCCGACGGCTATGCGCGCGCCACCGGCAAGCTCGGCGTCTGCGTGATC
ACCGCCGGGCCGGGCTTCACCAATGGCGTCACGGCGATTCTCAACGCGCAGCTCGACAGCATCCCGACGTTGTTCATCGT
CGGCTCGCCGCCGCTGCGCGAGGCCGAGACCAATGCGCTGCAGGGCGGCGTCGATCAGATCGCCGTCACGCTGCCGATGG
TGAAGTATGCGCATCGCATCACCAACACCGAACGCATTCCGGACCTTACCGCGTTGGCGATCCGCAAGGCCACGACGGGT
CGCCGCGGCGCGGTGCTGCTCGATGTGCCGATCGACGTGCTGCACATGCGCGCCCACAAGGACGACGTCCGTCCGGCGCA
CGGCCTCAATATAAATCCGCGTCCGGCGCCGCATCCGGACGAGATTAAGGCTGCCATCGCGATCCTGAAGGCGAGCGAGC
GGCCGGTGATCGTGGTTGGCGGTGAGGCGCGCTATTCGAATTGCCGCGACGACCTCGTCGCGCTAGCCGAGGCCACGCAG
ATTCCGGTGGTGTCGAACACCCGGGGCATGGGCGTGTTTCCGAGCAGCCATCCGCTGAACGGTCAGGTGGTCGGCAATCT
GGCACTGCTGTCGGCGCGGCGGCCGGACGCCGCGCTGCTACTCGGTACGCGATTCGGCTTCCGCCTCGCCGGCCGCAGCA
CCGCGTTCTTTCCCGACGATGCCAAGTTGATTCAGGTGCACAGCGACGCCGCCGAGCTCGGCCGGCTGCGCCATGTCGAA
CTGCCGATCGTCGCCGATGTCGGCGCCGCGCTAACCACGCTGCGTACCGCCGCCGCGGCCGAAACATGGCCGCAGCGTCC
GGCCTGGATCGAGGCGGTGACCAAGGCACCCGCCAAGATGCTCGACATGTTTCCGAACAAGCAAGGACCAAAAGGCATCC
ATCCGTTCTGGGCCGCGGAAGCCGCGGTCCGGGCGGCAGGGCCGGACGCCGCGATCGTGCTCGACGGCGGCGAGGCGGCG
AGCTGGGCCGCGTTCCATCTCCGCTCCAATCAGCCGGGCCACGTCCAGGGCGTCGGCTATCTCGGCTGCCTCGGCACCGG
GCCGGGCCAGTCGATCGGAGCGCAGTTGGTCGATCCGGACAAACGGGTAATGCAGATCACCGGCGACGGCGCGATGGGTT
TCCACATCCAGGAGTTCGATACGATGAGCCGGCATCGTCTGCCGATCTGTACCATCGTGCTCAACAACGAGATCTGGGGC
ATGTCGATCCACGGCCAGCAGATCATGTACGGCGCCAATTATAGCGCGATCAGCGAGCTACGCGGCACCAACTACGCCAG
CGTCGCGGCGGCGTTCGGGTGCCACGCCGAGCGCGTCACCGAATTCGACGAGATCGAGCCTGCGATCGCTCGTGCCTATG
CGTCGGGCCAGCCATCCTGCATCGAGATCATGATCGATCCGGACGTCGTGCATCCGGTCACCGTCGCGGCGCTCGGCACG
ATCAGCGACGAGAAGCGCGAGATCATGATCCCCTACTACGAGAACATCCCGATCCGTCCGCAGGCGTGA

Upstream 100 bases:

>100_bases
GGGCTGACTGCCCGAACCGGGCAGCCGAACTTCCTCGGGCACCTGGCGCCCAAGCCCTGAACCGCCCCTCCGCGCAGACC
AACGAGAAAAGGAAACACCC

Downstream 100 bases:

>100_bases
ACGGCTCACATCAGCGCGGAGGCGATCAGATGAAGGCTGCAGTGTTCGAGAAAACCGGGGAGCGGCTCGTCGTCCGCGAC
GTCGCCGCGCCGGCGCTGGG

Product: thiamine pyrophosphate protein TPP binding domain-containing protein

Products: NA

Alternate protein names: Benzoin aldolase; BL; BZL [H]

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MSAATSSAGDWLEGGELLARTLRDAGVDTVFALHGGHLESFYRGCIANDIRLVDFRHESSAGHAADGYARATGKLGVCVI
TAGPGFTNGVTAILNAQLDSIPTLFIVGSPPLREAETNALQGGVDQIAVTLPMVKYAHRITNTERIPDLTALAIRKATTG
RRGAVLLDVPIDVLHMRAHKDDVRPAHGLNINPRPAPHPDEIKAAIAILKASERPVIVVGGEARYSNCRDDLVALAEATQ
IPVVSNTRGMGVFPSSHPLNGQVVGNLALLSARRPDAALLLGTRFGFRLAGRSTAFFPDDAKLIQVHSDAAELGRLRHVE
LPIVADVGAALTTLRTAAAAETWPQRPAWIEAVTKAPAKMLDMFPNKQGPKGIHPFWAAEAAVRAAGPDAAIVLDGGEAA
SWAAFHLRSNQPGHVQGVGYLGCLGTGPGQSIGAQLVDPDKRVMQITGDGAMGFHIQEFDTMSRHRLPICTIVLNNEIWG
MSIHGQQIMYGANYSAISELRGTNYASVAAAFGCHAERVTEFDEIEPAIARAYASGQPSCIEIMIDPDVVHPVTVAALGT
ISDEKREIMIPYYENIPIRPQA

Sequences:

>Translated_582_residues
MSAATSSAGDWLEGGELLARTLRDAGVDTVFALHGGHLESFYRGCIANDIRLVDFRHESSAGHAADGYARATGKLGVCVI
TAGPGFTNGVTAILNAQLDSIPTLFIVGSPPLREAETNALQGGVDQIAVTLPMVKYAHRITNTERIPDLTALAIRKATTG
RRGAVLLDVPIDVLHMRAHKDDVRPAHGLNINPRPAPHPDEIKAAIAILKASERPVIVVGGEARYSNCRDDLVALAEATQ
IPVVSNTRGMGVFPSSHPLNGQVVGNLALLSARRPDAALLLGTRFGFRLAGRSTAFFPDDAKLIQVHSDAAELGRLRHVE
LPIVADVGAALTTLRTAAAAETWPQRPAWIEAVTKAPAKMLDMFPNKQGPKGIHPFWAAEAAVRAAGPDAAIVLDGGEAA
SWAAFHLRSNQPGHVQGVGYLGCLGTGPGQSIGAQLVDPDKRVMQITGDGAMGFHIQEFDTMSRHRLPICTIVLNNEIWG
MSIHGQQIMYGANYSAISELRGTNYASVAAAFGCHAERVTEFDEIEPAIARAYASGQPSCIEIMIDPDVVHPVTVAALGT
ISDEKREIMIPYYENIPIRPQA
>Mature_581_residues
SAATSSAGDWLEGGELLARTLRDAGVDTVFALHGGHLESFYRGCIANDIRLVDFRHESSAGHAADGYARATGKLGVCVIT
AGPGFTNGVTAILNAQLDSIPTLFIVGSPPLREAETNALQGGVDQIAVTLPMVKYAHRITNTERIPDLTALAIRKATTGR
RGAVLLDVPIDVLHMRAHKDDVRPAHGLNINPRPAPHPDEIKAAIAILKASERPVIVVGGEARYSNCRDDLVALAEATQI
PVVSNTRGMGVFPSSHPLNGQVVGNLALLSARRPDAALLLGTRFGFRLAGRSTAFFPDDAKLIQVHSDAAELGRLRHVEL
PIVADVGAALTTLRTAAAAETWPQRPAWIEAVTKAPAKMLDMFPNKQGPKGIHPFWAAEAAVRAAGPDAAIVLDGGEAAS
WAAFHLRSNQPGHVQGVGYLGCLGTGPGQSIGAQLVDPDKRVMQITGDGAMGFHIQEFDTMSRHRLPICTIVLNNEIWGM
SIHGQQIMYGANYSAISELRGTNYASVAAAFGCHAERVTEFDEIEPAIARAYASGQPSCIEIMIDPDVVHPVTVAALGTI
SDEKREIMIPYYENIPIRPQA

Specific function: Cleavage of benzoin-anisoin acyloin linkage [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=586, Percent_Identity=28.6689419795222, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI93004078, Length=558, Percent_Identity=23.8351254480287, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=30.9608540925267, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI87081685, Length=538, Percent_Identity=29.368029739777, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1788716, Length=546, Percent_Identity=28.5714285714286, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1786717, Length=576, Percent_Identity=28.6458333333333, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=26.6423357664234, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=583, Percent_Identity=28.3018867924528, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=25.1785714285714, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=25.1785714285714, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=567, Percent_Identity=26.8077601410935, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320816, Length=546, Percent_Identity=23.4432234432234, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6323073, Length=525, Percent_Identity=25.3333333333333, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=526, Percent_Identity=23.384030418251, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=524, Percent_Identity=24.618320610687, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=26.063829787234, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.2.38 [H]

Molecular weight: Translated: 61974; Mature: 61843

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAATSSAGDWLEGGELLARTLRDAGVDTVFALHGGHLESFYRGCIANDIRLVDFRHESS
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCC
AGHAADGYARATGKLGVCVITAGPGFTNGVTAILNAQLDSIPTLFIVGSPPLREAETNAL
CCCCCCCHHEECCCEEEEEEECCCCCCCCHHHEEECHHCCCCEEEEECCCCCCCCHHHHH
QGGVDQIAVTLPMVKYAHRITNTERIPDLTALAIRKATTGRRGAVLLDVPIDVLHMRAHK
HCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCC
DDVRPAHGLNINPRPAPHPDEIKAAIAILKASERPVIVVGGEARYSNCRDDLVALAEATQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCEEEECCCCCCCCCHHHHHHHHHHCC
IPVVSNTRGMGVFPSSHPLNGQVVGNLALLSARRPDAALLLGTRFGFRLAGRSTAFFPDD
CCEEECCCCCEECCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCEEECCCCCEECCCC
AKLIQVHSDAAELGRLRHVELPIVADVGAALTTLRTAAAAETWPQRPAWIEAVTKAPAKM
CEEEEEECCHHHHCCEEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHH
LDMFPNKQGPKGIHPFWAAEAAVRAAGPDAAIVLDGGEAASWAAFHLRSNQPGHVQGVGY
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEE
LGCLGTGPGQSIGAQLVDPDKRVMQITGDGAMGFHIQEFDTMSRHRLPICTIVLNNEIWG
EEEECCCCCCCCCCEEECCCCEEEEEECCCCEEEEEHHHHHHHHCCCCEEEEEECCCEEE
MSIHGQQIMYGANYSAISELRGTNYASVAAAFGCHAERVTEFDEIEPAIARAYASGQPSC
EEECCCEEEECCCHHHHHHHCCCCHHHHHHHHCCCHHHHCCHHHCCHHHHHHHCCCCCCE
IEIMIDPDVVHPVTVAALGTISDEKREIMIPYYENIPIRPQA
EEEEECCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SAATSSAGDWLEGGELLARTLRDAGVDTVFALHGGHLESFYRGCIANDIRLVDFRHESS
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCC
AGHAADGYARATGKLGVCVITAGPGFTNGVTAILNAQLDSIPTLFIVGSPPLREAETNAL
CCCCCCCHHEECCCEEEEEEECCCCCCCCHHHEEECHHCCCCEEEEECCCCCCCCHHHHH
QGGVDQIAVTLPMVKYAHRITNTERIPDLTALAIRKATTGRRGAVLLDVPIDVLHMRAHK
HCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCC
DDVRPAHGLNINPRPAPHPDEIKAAIAILKASERPVIVVGGEARYSNCRDDLVALAEATQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCEEEECCCCCCCCCHHHHHHHHHHCC
IPVVSNTRGMGVFPSSHPLNGQVVGNLALLSARRPDAALLLGTRFGFRLAGRSTAFFPDD
CCEEECCCCCEECCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCEEECCCCCEECCCC
AKLIQVHSDAAELGRLRHVELPIVADVGAALTTLRTAAAAETWPQRPAWIEAVTKAPAKM
CEEEEEECCHHHHCCEEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHH
LDMFPNKQGPKGIHPFWAAEAAVRAAGPDAAIVLDGGEAASWAAFHLRSNQPGHVQGVGY
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEE
LGCLGTGPGQSIGAQLVDPDKRVMQITGDGAMGFHIQEFDTMSRHRLPICTIVLNNEIWG
EEEECCCCCCCCCCEEECCCCEEEEEECCCCEEEEEHHHHHHHHCCCCEEEEEECCCEEE
MSIHGQQIMYGANYSAISELRGTNYASVAAAFGCHAERVTEFDEIEPAIARAYASGQPSC
EEECCCEEEECCCHHHHHHHCCCCHHHHHHHHCCCHHHHCCHHHCCHHHHHHHCCCCCCE
IEIMIDPDVVHPVTVAALGTISDEKREIMIPYYENIPIRPQA
EEEEECCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8026749 [H]