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The map label for this gene is purH [H]

Identifier: 192288462

GI number: 192288462

Start: 31529

End: 33121

Strand: Reverse

Name: purH [H]

Synonym: Rpal_0029

Alternate gene names: 192288462

Gene position: 33121-31529 (Counterclockwise)

Preceding gene: 192288463

Following gene: 192288459

Centisome position: 0.58

GC content: 66.35

Gene sequence:

>1593_bases
ATGACCCAGAATCCCCGTCGCGTCACCCGTGCCCTGTTGTCCGTGTCCGATAAGACCGGTCTGATCGATTTCGCCCGTGC
GCTCGCCGGCCATGGCGTCGAACTGGTTTCGACCGGTGGCACCGCCAAGGCGATCGCTGCGGCCGGCCTTCCGGTCAAGG
ACGTCTCCGAGCTGACCGGTTTCCCTGAGATGATGGATGGTCGGGTCAAGACGCTGCATCCGAAGGTGCATGGCGGCCTG
CTGGCGATTCGCGACAACGACGAACACACCCAGGCGATGGCCGCGCACGGCATCCCGCAGATCGACCTCCTGGTGGTGAA
CCTCTATCCGTTCGAAGCTACCGTCGACAAAGGCGCCTCTTACGAGGACTGCATCGAGAACATCGACATCGGCGGCCCGG
CGATGATCCGCGCCGCCGCCAAGAACCACGACGACGTCGCGGTTGTGGTCGAGGCCAGCGATTATCAGGCGGTGCTGGAT
GAGCTGACTGCCAACAACGGCGCCACCACGCTGCCACTGCGCAAGCGCCTCGCTGCCAAGGCCTATGCGCGCACGGCCGC
TTATGATGCGGCGATCTCCAACTGGTTCGCGCTGCAGCTCAAGACCGATGCGCCGGACTTCCGCGCGATCGGTGGACGGC
TGATCCAGAGCCTCCGCTACGGCGAAAATCCGCACCAGACCGCGGCGTTCTACGCCACGCCGGAGAAGCGTCCGGGCGTT
GCCACCGCGCGCCAGGTGCAGGGCAAGGAGCTGTCCTACAACAACATCAACGACACCGACGCCGCCTATGAGTGCGTCGG
CGAGTTCGACGCGGCGCGTACCGCGGCCTGCGTCATCGTCAAGCACGCCAATCCTTGCGGCGTCGCCGAGGGATCGAGCC
TGCTCGACGCCTACAAGAAGGCGCTGGCTTGCGACTCCGTCTCGGCGTTCGGAGGCATCGTCGCGCTCAACCGCACGCTC
GACGCCGAAGCGGCGCGCGCCATCACCGAGATCTTCACCGAAGTGATCATCGCGCCGGACGCCACCGACGAAGCGATCGC
GATCGTCGCGGCGAAGAAGAACCTGCGGCTGCTGCTGGCGGGCGCGCTGCCCGATCCGCGCGCCAACGGTCTGACCTACA
AGACCGTCGCCGGCGGCCTGCTGGTGCAGAGCCGCGACAATGCGGTGGTCGATGACATGGCGCTGAAGGTCGTCACCAAG
CGGCAGCCGACCGAAGCCGAGCTGCGTGACCTGAAGTTCGCGTTCCGCGTCGGCAAGCACGTCAAGTCCAACACCATCAT
CTATGCCAAGGACCTCGCCACTGTCGGTATCGGTGCCGGTCAGATGAGCCGCGTCGACTCCGCCCGCATCGCCGCCCGCA
AGGCGCAGGATGCGGCCGAGGCGATGAAGCTCGCCGCGCCGATGACCAAGGGCTCGGTGGTGGCCTCCGACGCGTTCTTC
CCGTTCGCCGACGGCATGCTGGCCTGTATCGAAGCCGGCGCCACCGCGGTGATCCAGCCCGGCGGCTCGGTTCGCGACGA
CGAAGTGATCAAGGCTGCGGACGACGCCGGCATCGCCATGGTGTTCACCGGCACCCGGCACTTCCGACACTAA

Upstream 100 bases:

>100_bases
GCGCGACTGGCGACGTTAGGATGGAGCACCATCGGGAAGCGCGGGACGTATCTGCCGCGCGCCTGGGCATAGCTTTCCTT
CCAACAGAAGATCGTGCCCC

Downstream 100 bases:

>100_bases
CAAAGGACGACGAATAGCGGGACAGCGACCGTTCGCGTTGCTGTCCCGCTTCGTTTTTCACGCCGCAGCACCTTTCTGCT
GAGCGCTTGCGGCGTGCCGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTQNPRRVTRALLSVSDKTGLIDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSELTGFPEMMDGRVKTLHPKVHGGL
LAIRDNDEHTQAMAAHGIPQIDLLVVNLYPFEATVDKGASYEDCIENIDIGGPAMIRAAAKNHDDVAVVVEASDYQAVLD
ELTANNGATTLPLRKRLAAKAYARTAAYDAAISNWFALQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGV
ATARQVQGKELSYNNINDTDAAYECVGEFDAARTAACVIVKHANPCGVAEGSSLLDAYKKALACDSVSAFGGIVALNRTL
DAEAARAITEIFTEVIIAPDATDEAIAIVAAKKNLRLLLAGALPDPRANGLTYKTVAGGLLVQSRDNAVVDDMALKVVTK
RQPTEAELRDLKFAFRVGKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAEAMKLAAPMTKGSVVASDAFF
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH

Sequences:

>Translated_530_residues
MTQNPRRVTRALLSVSDKTGLIDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSELTGFPEMMDGRVKTLHPKVHGGL
LAIRDNDEHTQAMAAHGIPQIDLLVVNLYPFEATVDKGASYEDCIENIDIGGPAMIRAAAKNHDDVAVVVEASDYQAVLD
ELTANNGATTLPLRKRLAAKAYARTAAYDAAISNWFALQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGV
ATARQVQGKELSYNNINDTDAAYECVGEFDAARTAACVIVKHANPCGVAEGSSLLDAYKKALACDSVSAFGGIVALNRTL
DAEAARAITEIFTEVIIAPDATDEAIAIVAAKKNLRLLLAGALPDPRANGLTYKTVAGGLLVQSRDNAVVDDMALKVVTK
RQPTEAELRDLKFAFRVGKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAEAMKLAAPMTKGSVVASDAFF
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH
>Mature_529_residues
TQNPRRVTRALLSVSDKTGLIDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSELTGFPEMMDGRVKTLHPKVHGGLL
AIRDNDEHTQAMAAHGIPQIDLLVVNLYPFEATVDKGASYEDCIENIDIGGPAMIRAAAKNHDDVAVVVEASDYQAVLDE
LTANNGATTLPLRKRLAAKAYARTAAYDAAISNWFALQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGVA
TARQVQGKELSYNNINDTDAAYECVGEFDAARTAACVIVKHANPCGVAEGSSLLDAYKKALACDSVSAFGGIVALNRTLD
AEAARAITEIFTEVIIAPDATDEAIAIVAAKKNLRLLLAGALPDPRANGLTYKTVAGGLLVQSRDNAVVDDMALKVVTKR
QPTEAELRDLKFAFRVGKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAEAMKLAAPMTKGSVVASDAFFP
FADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=471, Percent_Identity=40.1273885350318, Blast_Score=279, Evalue=5e-75,
Organism=Escherichia coli, GI1790439, Length=541, Percent_Identity=58.2255083179298, Blast_Score=578, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71985564, Length=603, Percent_Identity=33.3333333333333, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI71985574, Length=305, Percent_Identity=28.5245901639344, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323056, Length=607, Percent_Identity=33.4431630971993, Blast_Score=283, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6323768, Length=472, Percent_Identity=37.0762711864407, Blast_Score=270, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24649832, Length=600, Percent_Identity=36.8333333333333, Blast_Score=308, Evalue=6e-84,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55979; Mature: 55848

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNPRRVTRALLSVSDKTGLIDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSELTG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPKVHGGLLAIRDNDEHTQAMAAHGIPQIDLLVVNLYPFEATVDKGAS
CHHHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEECCCHHHCCCCC
YEDCIENIDIGGPAMIRAAAKNHDDVAVVVEASDYQAVLDELTANNGATTLPLRKRLAAK
HHHHHHHCCCCCHHHHEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEHHHHHHHHHH
AYARTAAYDAAISNWFALQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGV
HHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
ATARQVQGKELSYNNINDTDAAYECVGEFDAARTAACVIVKHANPCGVAEGSSLLDAYKK
CHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHH
ALACDSVSAFGGIVALNRTLDAEAARAITEIFTEVIIAPDATDEAIAIVAAKKNLRLLLA
HHHHCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCEEEEE
GALPDPRANGLTYKTVAGGLLVQSRDNAVVDDMALKVVTKRQPTEAELRDLKFAFRVGKH
ECCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
VKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAEAMKLAAPMTKGSVVASDAFF
CCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH
CHHHHHHHHHHCCCEEEECCCCCCCCCHHHEECCCCCEEEEEECCHHCCC
>Mature Secondary Structure 
TQNPRRVTRALLSVSDKTGLIDFARALAGHGVELVSTGGTAKAIAAAGLPVKDVSELTG
CCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPKVHGGLLAIRDNDEHTQAMAAHGIPQIDLLVVNLYPFEATVDKGAS
CHHHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEEECCCHHHCCCCC
YEDCIENIDIGGPAMIRAAAKNHDDVAVVVEASDYQAVLDELTANNGATTLPLRKRLAAK
HHHHHHHCCCCCHHHHEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEHHHHHHHHHH
AYARTAAYDAAISNWFALQLKTDAPDFRAIGGRLIQSLRYGENPHQTAAFYATPEKRPGV
HHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
ATARQVQGKELSYNNINDTDAAYECVGEFDAARTAACVIVKHANPCGVAEGSSLLDAYKK
CHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHH
ALACDSVSAFGGIVALNRTLDAEAARAITEIFTEVIIAPDATDEAIAIVAAKKNLRLLLA
HHHHCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCEEEEE
GALPDPRANGLTYKTVAGGLLVQSRDNAVVDDMALKVVTKRQPTEAELRDLKFAFRVGKH
ECCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
VKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAAEAMKLAAPMTKGSVVASDAFF
CCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
PFADGMLACIEAGATAVIQPGGSVRDDEVIKAADDAGIAMVFTGTRHFRH
CHHHHHHHHHHCCCEEEECCCCCCCCCHHHEECCCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]